Restriction map for
pF1KB8590
(Circular) (Six-base) MAPSORT of: pF1KB8590.seq Check: 4380 from: 1 to: 4148
pF1KB8590 4148 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1186 1186
Size: 4148
Acc65I G'GTAC_C
Cuts at: 1164 1164
Size: 4148
AclI AA'CG_TT
Cuts at: 3988 3988
Size: 4148
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1973 2405 2758 3420 1973
Size: 432 353 662 2701
Fragments arranged by size:
2701 662 432 353
AflIII A'CryG_T
Cuts at: 1500 3300 3581 1500
Size: 1800 281 2067
Fragments arranged by size:
2067 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1453 2891 1453
Size: 1438 2710
ApaLI G'TGCA_C
Cuts at: 842 2986 842
Size: 2144 2004
ApoI r'AATT_y
Cuts at: 195 476 1152 3501 3757 195
Size: 281 676 2349 256 586
Fragments arranged by size:
2349 676 586 281 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4148
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
AvaI C'yCGr_G
Cuts at: 1168 1168
Size: 4148
BamHI G'GATC_C
Cuts at: 263 1173 263
Size: 910 3238
BanI G'GyrC_C
Cuts at: 922 1164 1578 1826 1861 922
Size: 242 414 248 35 3209
Fragments arranged by size:
3209 414 248 242 35
BanII G_rGCy'C
Cuts at: 664 1162 2192 664
Size: 498 1030 2620
Fragments arranged by size:
2620 1030 498
BbeI G_GCGC'C
Cuts at: 1582 1830 1582
Size: 248 3900
BciVI GTATCCnnnnn_n'
Cuts at: 1074 2046 3102 4148 1074
Size: 972 1056 1046 1074
Fragments arranged by size:
1074 1056 1046 972
BclI T'GATC_A
Cuts at: 339 1668 339
Size: 1329 2819
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4148
BglII A'GATC_T
Cuts at: 1663 3700 1663
Size: 2037 2111
BlpI GC'TnA_GC
Cuts at: 1260 1260
Size: 4148
Bme1580I G_kGCm'C
Cuts at: 846 1773 1866 2990 846
Size: 927 93 1124 2004
Fragments arranged by size:
2004 1124 927 93
BmrI ACTGGGnnnn_n'
Cuts at: 812 1360 1441 1776 3920 812
Size: 548 81 335 2144 1040
Fragments arranged by size:
2144 1040 548 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2515 2515
Size: 4148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 1145 386
Size: 759 3389
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1324 2203 2917 3215 1324
Size: 879 714 298 2257
Fragments arranged by size:
2257 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 871 3852 871
Size: 2981 1167
BsaAI yAC'GTr
Cuts at: 1501 1501
Size: 4148
BsaBI GATnn'nnATC
Cuts at: 6 1662 3699 6
Size: 1656 2037 455
Fragments arranged by size:
2037 1656 455
BsaHI Gr'CG_yC
Cuts at: 1579 1827 2529 4025 1579
Size: 248 702 1496 1702
Fragments arranged by size:
1702 1496 702 248
BsaWI w'CCGG_w
Cuts at: 134 1337 1858 2536 2947 3094 3368 134
Size: 1203 521 678 411 147 274 914
Fragments arranged by size:
1203 914 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 498 528 498
Size: 30 4118
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 713 713
Size: 4148
BseYI C'CCAG_C
Cuts at: 162 1573 2331 2996 3404 162
Size: 1411 758 665 408 906
Fragments arranged by size:
1411 906 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 731 1031 731
Size: 300 3848
BsiEI CG_ry'CG
Cuts at: 104 1690 1736 2966 104
Size: 1586 46 1230 1286
Fragments arranged by size:
1586 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 846 1162 1940 2130 2990 846
Size: 316 778 190 860 2004
Fragments arranged by size:
2004 860 778 316 190
BsmI GAATG_Cn'
Cuts at: 635 635
Size: 4148
Bsp1286I G_dGCh'C
Cuts at: 664 846 1162 1773 1866 1940 2130 2192 2990
Size: 182 316 611 93 74 190 62 798
Cuts at: 2990 664
Size: 1822
Fragments arranged by size:
1822 798 611 316 190 182 93 74
62
BspEI T'CCGG_A
Cuts at: 134 134
Size: 4148
BspHI T'CATG_A
Cuts at: 560 569 2580 560
Size: 9 2011 2128
Fragments arranged by size:
2128 2011 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1198 1412 1714 2095 1198
Size: 214 302 381 3251
Fragments arranged by size:
3251 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2440 2494 3978 4143 50
Size: 2390 54 1484 165 55
Fragments arranged by size:
2390 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 950 2060 950
Size: 1110 3038
BsrFI r'CCGG_y
Cuts at: 2146 2327 2146
Size: 181 3967
BssSI C'ACGA_G
Cuts at: 2419 3127 2419
Size: 708 3440
BstAPI GCAn_nnn'nTGC
Cuts at: 1525 1525
Size: 4148
BstBI TT'CG_AA
Cuts at: 2509 2509
Size: 4148
BstYI r'GATC_y
Cuts at: 1 263 889 1173 1640 1663 1998 2244 2550
Size: 262 626 284 467 23 335 246 306
Cuts at: 2550 2562 2648 3700 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 626 467 449 335 306 284 262
246 86 23 12
BtgI C'CryG_G
Cuts at: 614 914 614
Size: 300 3848
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2098 2279 2442 2098
Size: 181 163 3804
Fragments arranged by size:
3804 181 163
BtsI GCAGTG_nn'
Cuts at: 719 1391 719
Size: 672 3476
ClaI AT'CG_AT
Cuts at: 316 316
Size: 4148
DraI TTT'AAA
Cuts at: 333 1147 333
Size: 814 3334
DrdI GACnn_nn'nnGTC
Cuts at: 1854 3198 1854
Size: 1344 2804
EaeI y'GGCC_r
Cuts at: 282 803 1733 1907 2298 2325 3670 282
Size: 521 930 174 391 27 1345 760
Fragments arranged by size:
1345 930 760 521 391 174 27
EagI C'GGCC_G
Cuts at: 1733 1733
Size: 4148
EarI CTCTTCn'nnn_
Cuts at: 752 2171 2381 752
Size: 1419 210 2519
Fragments arranged by size:
2519 1419 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2356 3098 3244 3958 2356
Size: 742 146 714 2546
Fragments arranged by size:
2546 742 714 146
EcoICRI GAG'CTC
Cuts at: 1160 1160
Size: 4148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 1145 1973 2405 2758 3420 386
Size: 759 828 432 353 662 1114
Fragments arranged by size:
1114 828 759 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 742 1287 742
Size: 545 3603
EcoRI G'AATT_C
Cuts at: 195 476 1152 195
Size: 281 676 3191
Fragments arranged by size:
3191 676 281
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2167 2167
Size: 4148
FspI TGC'GCA
Cuts at: 1929 1929
Size: 4148
HaeII r_GCGC'y
Cuts at: 1582 1830 3060 3831 1582
Size: 248 1230 771 1899
Fragments arranged by size:
1899 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1990 2020 2022 2052 1990
Size: 30 2 30 4086
Fragments arranged by size:
4086 30 30 2
HincII GTy'rAC
Cuts at: 224 1187 224
Size: 963 3185
HpaI GTT'AAC
Cuts at: 224 224
Size: 4148
KasI G'GCGC_C
Cuts at: 1578 1826 1578
Size: 248 3900
KpnI G_GTAC'C
Cuts at: 1168 1168
Size: 4148
MfeI C'AATT_G
Cuts at: 593 593
Size: 4148
MluI A'CGCG_T
Cuts at: 3581 3581
Size: 4148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 600 2907 3091 600
Size: 2307 184 1657
Fragments arranged by size:
2307 1657 184
MscI TGG'CCA
Cuts at: 284 805 1909 284
Size: 521 1104 2523
Fragments arranged by size:
2523 1104 521
MslI CAynn'nnrTG
Cuts at: 490 698 2137 2264 490
Size: 208 1439 127 2374
Fragments arranged by size:
2374 1439 208 127
MspA1I CmG'CkG
Cuts at: 775 1259 1573 1933 2717 2962 775
Size: 484 314 360 784 245 1961
Fragments arranged by size:
1961 784 484 360 314 245
NaeI GCC'GGC
Cuts at: 2329 2329
Size: 4148
NarI GG'CG_CC
Cuts at: 1579 1827 1579
Size: 248 3900
NcoI C'CATG_G
Cuts at: 614 614
Size: 4148
NdeI CA'TA_TG
Cuts at: 651 651
Size: 4148
NgoMIV G'CCGG_C
Cuts at: 2327 2327
Size: 4148
NspI r_CATG'y
Cuts at: 975 1201 1701 3304 975
Size: 226 500 1603 1819
Fragments arranged by size:
1819 1603 500 226
PasI CC'CwG_GG
Cuts at: 849 849
Size: 4148
PciI A'CATG_T
Cuts at: 3300 3300
Size: 4148
PflMI CCAn_nnn'nTGG
Cuts at: 1030 1030
Size: 4148
PmeI GTTT'AAAC
Cuts at: 1147 1147
Size: 4148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2594 2626 2594
Size: 32 4116
PpuMI rG'GwC_Cy
Cuts at: 742 742
Size: 4148
PstI C_TGCA'G
Cuts at: 1195 1880 1195
Size: 685 3463
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4148
PvuII CAG'CTG
Cuts at: 775 1573 1933 775
Size: 798 360 2990
Fragments arranged by size:
2990 798 360
RsrII CG'GwC_CG
Cuts at: 2343 2343
Size: 4148
SacI G_AGCT'C
Cuts at: 1162 1162
Size: 4148
SalI G'TCGA_C
Cuts at: 1185 1185
Size: 4148
SapI GCTCTTCn'nnn_
Cuts at: 2171 2381 2171
Size: 210 3938
SbfI CC_TGCA'GG
Cuts at: 1195 1195
Size: 4148
ScaI AGT'ACT
Cuts at: 3665 3665
Size: 4148
SfcI C'TryA_G
Cuts at: 33 1191 1876 2844 3035 33
Size: 1158 685 968 191 1146
Fragments arranged by size:
1158 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1580 1828 1580
Size: 248 3900
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
SmaI CCC'GGG
Cuts at: 1170 1170
Size: 4148
SmlI C'TyrA_G
Cuts at: 1303 2218 2932 3194 1303
Size: 915 714 262 2257
Fragments arranged by size:
2257 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4148
SphI G_CATG'C
Cuts at: 1201 1701 1201
Size: 500 3648
SspI AAT'ATT
Cuts at: 734 734
Size: 4148
StuI AGG'CCT
Cuts at: 178 791 178
Size: 613 3535
StyI C'CwwG_G
Cuts at: 173 614 1282 1636 173
Size: 441 668 354 2685
Fragments arranged by size:
2685 668 441 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 176 576 697 829 1731 1864 2323 2532 176
Size: 400 121 132 902 133 459 209 1792
Fragments arranged by size:
1792 902 459 400 209 133 132 121
TatI w'GTAC_w
Cuts at: 494 3663 494
Size: 3169 979
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 255 287 3665 3697 255
Size: 32 3378 32 706
Fragments arranged by size:
3378 706 32 32
Tth111I GACn'n_nGTC
Cuts at: 1945 1945
Size: 4148
XbaI T'CTAG_A
Cuts at: 1179 1179
Size: 4148
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1643 1643
Size: 4148
XmaI C'CCGG_G
Cuts at: 1168 1168
Size: 4148
XmnI GAAnn'nnTTC
Cuts at: 480 827 480
Size: 347 3801
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI
BfrBI BmgBI BmtI Bpu10I BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII NheI NotI NruI NsiI PacI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
ZraI