Restriction map for
pF1KB8588
(Circular) (Six-base) MAPSORT of: pF1KB8588.seq Check: 8856 from: 1 to: 6701
pF1KB8588 6701 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1823 3739 1823
Size: 1916 4785
Acc65I G'GTAC_C
Cuts at: 3717 3717
Size: 6701
AclI AA'CG_TT
Cuts at: 2547 6541 2547
Size: 3994 2707
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 248 397 719 893 911 1750 2390 3549
Size: 22 149 322 174 18 839 640 1159
Cuts at: 3549 4526 4958 5311 5973 226
Size: 977 432 353 662 954
Fragments arranged by size:
1159 977 954 839 662 640 432 353
322 174 149 22 18
AflII C'TTAA_G
Cuts at: 166 1171 1464 1543 166
Size: 1005 293 79 5324
Fragments arranged by size:
5324 1005 293 79
AflIII A'CryG_T
Cuts at: 4053 5853 6134 4053
Size: 1800 281 4620
Fragments arranged by size:
4620 1800 281
AleI CACnn'nnGTG
Cuts at: 3070 3070
Size: 6701
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 702 702
Size: 6701
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1706 1738 3305 3337 1706
Size: 32 1567 32 5070
Fragments arranged by size:
5070 1567 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1834 4006 5444 1834
Size: 2172 1438 3091
Fragments arranged by size:
3091 2172 1438
ApaLI G'TGCA_C
Cuts at: 2458 5539 2458
Size: 3081 3620
ApoI r'AATT_y
Cuts at: 1208 1392 2027 2218 2507 3001 3705 6054 6310
Size: 184 635 191 289 494 704 2349 256
Cuts at: 6310 1208
Size: 1599
Fragments arranged by size:
2349 1599 704 635 494 289 256 191
184
AseI AT'TA_AT
Cuts at: 20 2438 20
Size: 2418 4283
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6701
AvaI C'yCGr_G
Cuts at: 3721 3721
Size: 6701
BamHI G'GATC_C
Cuts at: 3726 3726
Size: 6701
BanI G'GyrC_C
Cuts at: 504 3717 4131 4379 4414 504
Size: 3213 414 248 35 2791
Fragments arranged by size:
3213 2791 414 248 35
BanII G_rGCy'C
Cuts at: 3715 4745 3715
Size: 1030 5671
BbeI G_GCGC'C
Cuts at: 4135 4383 4135
Size: 248 6453
BbsI GAAGACnn'nnnn_
Cuts at: 470 828 983 1033 3371 470
Size: 358 155 50 2338 3800
Fragments arranged by size:
3800 2338 358 155 50
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6667
BciVI GTATCCnnnnn_n'
Cuts at: 1906 3636 4599 5655 6701 1906
Size: 1730 963 1056 1046 1906
Fragments arranged by size:
1906 1730 1056 1046 963
BclI T'GATC_A
Cuts at: 603 2103 4221 603
Size: 1500 2118 3083
Fragments arranged by size:
3083 2118 1500
BglII A'GATC_T
Cuts at: 4216 6253 4216
Size: 2037 4664
BlpI GC'TnA_GC
Cuts at: 3813 3813
Size: 6701
Bme1580I G_kGCm'C
Cuts at: 509 2462 4326 4419 5543 509
Size: 1953 1864 93 1124 1667
Fragments arranged by size:
1953 1864 1667 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 504 3913 3994 4329 6473 504
Size: 3409 81 335 2144 732
Fragments arranged by size:
3409 2144 732 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1833 5068 1833
Size: 3235 3466
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3503 3503
Size: 6701
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3164 3877 4756 5470 5768 3164
Size: 713 879 714 298 4097
Fragments arranged by size:
4097 879 714 713 298
BsaI GGTCTCn'nnnn_
Cuts at: 6405 6405
Size: 6701
BsaAI yAC'GTr
Cuts at: 1701 4054 1701
Size: 2353 4348
BsaBI GATnn'nnATC
Cuts at: 6 935 2006 4215 6252 6
Size: 929 1071 2209 2037 455
Fragments arranged by size:
2209 2037 1071 929 455
BsaHI Gr'CG_yC
Cuts at: 4132 4380 5082 6578 4132
Size: 248 702 1496 4255
Fragments arranged by size:
4255 1496 702 248
BsaWI w'CCGG_w
Cuts at: 534 2594 3890 4411 5089 5500 5647 5921 534
Size: 2060 1296 521 678 411 147 274 1314
Fragments arranged by size:
2060 1314 1296 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2646 2676 2723 2753 2646
Size: 30 47 30 6594
Fragments arranged by size:
6594 47 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 3515 3515
Size: 6701
BseYI C'CCAG_C
Cuts at: 1322 2876 4126 4884 5549 5957 1322
Size: 1554 1250 758 665 408 2066
Fragments arranged by size:
2066 1554 1250 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 4243 4289 5519 104
Size: 4139 46 1230 1286
Fragments arranged by size:
4139 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 388 2462 3715 4493 4683 5543 388
Size: 2074 1253 778 190 860 1546
Fragments arranged by size:
2074 1546 1253 860 778 190
BsmI GAATG_Cn'
Cuts at: 2815 2815
Size: 6701
Bsp1286I G_dGCh'C
Cuts at: 388 509 2462 3715 4326 4419 4493 4683 4745
Size: 121 1953 1253 611 93 74 190 62
Cuts at: 4745 5543 388
Size: 798 1546
Fragments arranged by size:
1953 1546 1253 798 611 190 121 93
74 62
BspEI T'CCGG_A
Cuts at: 2594 2594
Size: 6701
BspHI T'CATG_A
Cuts at: 1077 2045 5133 1077
Size: 968 3088 2645
Fragments arranged by size:
3088 2645 968
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3751 3965 4267 4648 3751
Size: 214 302 381 5804
Fragments arranged by size:
5804 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1751 3178 4993 5047 6531 6696 50
Size: 1701 1427 1815 54 1484 165 55
Fragments arranged by size:
1815 1701 1484 1427 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4613 4613
Size: 6701
BsrFI r'CCGG_y
Cuts at: 4699 4880 4699
Size: 181 6520
BsrGI T'GTAC_A
Cuts at: 2398 2398
Size: 6701
BssSI C'ACGA_G
Cuts at: 387 4972 5680 387
Size: 4585 708 1408
Fragments arranged by size:
4585 1408 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1990 2821 4078 1990
Size: 831 1257 4613
Fragments arranged by size:
4613 1257 831
BstBI TT'CG_AA
Cuts at: 5062 5062
Size: 6701
BstXI CCAn_nnnn'nTGG
Cuts at: 1513 1513
Size: 6701
BstYI r'GATC_y
Cuts at: 1 2589 3726 4193 4216 4551 4797 5103 5115
Size: 2588 1137 467 23 335 246 306 12
Cuts at: 5115 5201 6253 1
Size: 86 1052 449
Fragments arranged by size:
2588 1137 1052 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3491 3491
Size: 6701
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3310 4651 4832 4995 3310
Size: 1341 181 163 5016
Fragments arranged by size:
5016 1341 181 163
BtsI GCAGTG_nn'
Cuts at: 807 2135 3944 807
Size: 1328 1809 3564
Fragments arranged by size:
3564 1809 1328
ClaI AT'CG_AT
Cuts at: 3303 3303
Size: 6701
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3257 3292 3257
Size: 35 6666
DraI TTT'AAA
Cuts at: 1338 3700 1338
Size: 2362 4339
DrdI GACnn_nn'nnGTC
Cuts at: 4407 5751 4407
Size: 1344 5357
EaeI y'GGCC_r
Cuts at: 2061 4286 4460 4851 4878 6223 2061
Size: 2225 174 391 27 1345 2539
Fragments arranged by size:
2539 2225 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4286 4286
Size: 6701
EarI CTCTTCn'nnn_
Cuts at: 376 630 635 650 1769 1838 1918 4724 4934
Size: 254 5 15 1119 69 80 2806 210
Cuts at: 4934 376
Size: 2143
Fragments arranged by size:
2806 2143 1119 254 210 80 69 15
5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4909 5651 5797 6511 4909
Size: 742 146 714 5099
Fragments arranged by size:
5099 742 714 146
EcoICRI GAG'CTC
Cuts at: 3713 3713
Size: 6701
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 248 397 719 893 911 1750 2390 3503
Size: 22 149 322 174 18 839 640 1113
Cuts at: 3503 3549 4526 4958 5311 5973 226
Size: 46 977 432 353 662 954
Fragments arranged by size:
1113 977 954 839 662 640 432 353
322 174 149 46 22 18
EcoO109I rG'GnC_Cy
Cuts at: 3840 3840
Size: 6701
EcoRI G'AATT_C
Cuts at: 2507 3705 2507
Size: 1198 5503
EcoRV GAT'ATC
Cuts at: 2008 2008
Size: 6701
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3257 4720 3257
Size: 1463 5238
FspI TGC'GCA
Cuts at: 4482 4482
Size: 6701
HaeII r_GCGC'y
Cuts at: 4135 4383 5613 6384 4135
Size: 248 1230 771 4452
Fragments arranged by size:
4452 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3177 3209 3323 3355 4543 4573 4575 4605 3177
Size: 32 114 32 1188 30 2 30 5273
Fragments arranged by size:
5273 1188 114 32 32 30 30 2
HincII GTy'rAC
Cuts at: 515 755 3099 3740 515
Size: 240 2344 641 3476
Fragments arranged by size:
3476 2344 641 240
HindIII A'AGCT_T
Cuts at: 1546 1546
Size: 6701
KasI G'GCGC_C
Cuts at: 4131 4379 4131
Size: 248 6453
KpnI G_GTAC'C
Cuts at: 3721 3721
Size: 6701
MluI A'CGCG_T
Cuts at: 6134 6134
Size: 6701
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2343 5460 5644 2343
Size: 3117 184 3400
Fragments arranged by size:
3400 3117 184
MscI TGG'CCA
Cuts at: 2063 4462 2063
Size: 2399 4302
MslI CAynn'nnrTG
Cuts at: 684 941 1653 2537 3070 4690 4817 684
Size: 257 712 884 533 1620 127 2568
Fragments arranged by size:
2568 1620 884 712 533 257 127
MspA1I CmG'CkG
Cuts at: 118 2230 3812 4126 4486 5270 5515 118
Size: 2112 1582 314 360 784 245 1304
Fragments arranged by size:
2112 1582 1304 784 360 314 245
NaeI GCC'GGC
Cuts at: 4882 4882
Size: 6701
NarI GG'CG_CC
Cuts at: 4132 4380 4132
Size: 248 6453
NdeI CA'TA_TG
Cuts at: 1329 2381 1329
Size: 1052 5649
NgoMIV G'CCGG_C
Cuts at: 4880 4880
Size: 6701
NspI r_CATG'y
Cuts at: 987 3171 3754 4254 5857 987
Size: 2184 583 500 1603 1831
Fragments arranged by size:
2184 1831 1603 583 500
PasI CC'CwG_GG
Cuts at: 1879 1879
Size: 6701
PciI A'CATG_T
Cuts at: 5853 5853
Size: 6701
PflMI CCAn_nnn'nTGG
Cuts at: 2147 2926 2147
Size: 779 5922
PmeI GTTT'AAAC
Cuts at: 3700 3700
Size: 6701
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5147 5179 5147
Size: 32 6669
PshAI GACnn'nnGTC
Cuts at: 1173 1173
Size: 6701
PstI C_TGCA'G
Cuts at: 3748 4433 3748
Size: 685 6016
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6701
PvuII CAG'CTG
Cuts at: 118 2230 4126 4486 118
Size: 2112 1896 360 2333
Fragments arranged by size:
2333 2112 1896 360
RsrII CG'GwC_CG
Cuts at: 4896 4896
Size: 6701
SacI G_AGCT'C
Cuts at: 3715 3715
Size: 6701
SalI G'TCGA_C
Cuts at: 3738 3738
Size: 6701
SapI GCTCTTCn'nnn_
Cuts at: 376 1769 4724 4934 376
Size: 1393 2955 210 2143
Fragments arranged by size:
2955 2143 1393 210
SbfI CC_TGCA'GG
Cuts at: 3748 3748
Size: 6701
ScaI AGT'ACT
Cuts at: 2574 2655 6218 2574
Size: 81 3563 3057
Fragments arranged by size:
3563 3057 81
SfcI C'TryA_G
Cuts at: 33 113 3744 4429 5397 5588 33
Size: 80 3631 685 968 191 1146
Fragments arranged by size:
3631 1146 968 685 191 80
SfoI GGC'GCC
Cuts at: 4133 4381 4133
Size: 248 6453
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6701
SmaI CCC'GGG
Cuts at: 3723 3723
Size: 6701
SmlI C'TyrA_G
Cuts at: 166 1171 1464 1543 3179 3856 4771 5485 5747
Size: 1005 293 79 1636 677 915 714 262
Cuts at: 5747 166
Size: 1120
Fragments arranged by size:
1636 1120 1005 915 714 677 293 262
79
SnaBI TAC'GTA
Cuts at: 1701 1701
Size: 6701
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6701
SphI G_CATG'C
Cuts at: 987 3754 4254 987
Size: 2767 500 3434
Fragments arranged by size:
3434 2767 500
SspI AAT'ATT
Cuts at: 1976 1976
Size: 6701
StyI C'CwwG_G
Cuts at: 3627 3835 4189 3627
Size: 208 354 6139
Fragments arranged by size:
6139 354 208
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1211 2787 4284 4417 4876 5085 1211
Size: 1576 1497 133 459 209 2827
Fragments arranged by size:
2827 1576 1497 459 209 133
TatI w'GTAC_w
Cuts at: 856 2398 2572 2653 6216 856
Size: 1542 174 81 3563 1341
Fragments arranged by size:
3563 1542 1341 174 81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6218 6250 6218
Size: 32 6669
Tth111I GACn'n_nGTC
Cuts at: 1135 4498 1135
Size: 3363 3338
XbaI T'CTAG_A
Cuts at: 3732 3732
Size: 6701
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4196 4196
Size: 6701
XmaI C'CCGG_G
Cuts at: 3721 3721
Size: 6701
XmnI GAAnn'nnTTC
Cuts at: 1394 1394
Size: 6701
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsgI
BsiWI BsmBI BssHII BstEII BstZ17I BtgI DraIII EcoNI
FseI FspAI HpaI MfeI NcoI NheI NotI NruI
NsiI PacI PfoI PmlI PpuMI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI StuI
SwaI XhoI ZraI