Restriction map for
pF1KB8581
(Circular) (Six-base) MAPSORT of: pF1KB8581.seq Check: 6271 from: 1 to: 4679
pF1KB8581 4679 bp
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 198 669 697 198
Size: 471 28 4180
Fragments arranged by size:
4180 471 28
AccI GT'mk_AC
Cuts at: 1717 1717
Size: 4679
Acc65I G'GTAC_C
Cuts at: 1695 1695
Size: 4679
AclI AA'CG_TT
Cuts at: 4519 4519
Size: 4679
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 858 2504 2936 3289 3951 858
Size: 1646 432 353 662 1586
Fragments arranged by size:
1646 1586 662 432 353
AflIII A'CryG_T
Cuts at: 1484 2031 3831 4112 1484
Size: 547 1800 281 2051
Fragments arranged by size:
2051 1800 547 281
AleI CACnn'nnGTG
Cuts at: 550 1563 550
Size: 1013 3666
AlwNI CAG_nnn'CTG
Cuts at: 1030 1984 3422 1030
Size: 954 1438 2287
Fragments arranged by size:
2287 1438 954
ApaI G_GGCC'C
Cuts at: 141 764 1497 141
Size: 623 733 3323
Fragments arranged by size:
3323 733 623
ApaLI G'TGCA_C
Cuts at: 3517 3517
Size: 4679
ApoI r'AATT_y
Cuts at: 639 984 1683 4032 4288 639
Size: 345 699 2349 256 1030
Fragments arranged by size:
2349 1030 699 345 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4679
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4679
AvaI C'yCGr_G
Cuts at: 1699 1699
Size: 4679
BamHI G'GATC_C
Cuts at: 1010 1704 1010
Size: 694 3985
BanI G'GyrC_C
Cuts at: 657 709 847 885 904 999 1695 2109 2357
Size: 52 138 38 19 95 696 414 248
Cuts at: 2357 2392 657
Size: 35 2944
Fragments arranged by size:
2944 696 414 248 138 95 52 38
35 19
BanII G_rGCy'C
Cuts at: 141 246 764 1286 1333 1497 1693 2723 141
Size: 105 518 522 47 164 196 1030 2097
Fragments arranged by size:
2097 1030 522 518 196 164 105 47
BbeI G_GCGC'C
Cuts at: 851 1003 2113 2361 851
Size: 152 1110 248 3169
Fragments arranged by size:
3169 1110 248 152
BbsI GAAGACnn'nnnn_
Cuts at: 1325 1325
Size: 4679
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1185 1219 1185
Size: 34 4645
BciVI GTATCCnnnnn_n'
Cuts at: 2577 3633 4679 2577
Size: 1056 1046 2577
Fragments arranged by size:
2577 1056 1046
BclI T'GATC_A
Cuts at: 1115 2199 1115
Size: 1084 3595
BglII A'GATC_T
Cuts at: 1526 2194 4231 1526
Size: 668 2037 1974
Fragments arranged by size:
2037 1974 668
BlpI GC'TnA_GC
Cuts at: 630 1327 1791 630
Size: 697 464 3518
Fragments arranged by size:
3518 697 464
Bme1580I G_kGCm'C
Cuts at: 141 511 764 1497 2304 2397 3521 141
Size: 370 253 733 807 93 1124 1299
Fragments arranged by size:
1299 1124 807 733 370 253 93
BmgBI CAC'GTC
Cuts at: 1487 1487
Size: 4679
BmrI ACTGGGnnnn_n'
Cuts at: 143 767 1891 1972 2307 4451 143
Size: 624 1124 81 335 2144 371
Fragments arranged by size:
2144 1124 624 371 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3046 3046
Size: 4679
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 483 542 593 911 1126 1147 1153 1357
Size: 238 59 51 318 215 21 6 204
Cuts at: 1357 245
Size: 3567
Fragments arranged by size:
3567 318 238 215 204 59 51 21
6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 423 1423 1444 1855 2734 3448 3746 423
Size: 1000 21 411 879 714 298 1356
Fragments arranged by size:
1356 1000 879 714 411 298 21
BsaI GGTCTCn'nnnn_
Cuts at: 4383 4383
Size: 4679
BsaAI yAC'GTr
Cuts at: 2032 2032
Size: 4679
BsaBI GATnn'nnATC
Cuts at: 6 2193 4230 6
Size: 2187 2037 455
Fragments arranged by size:
2187 2037 455
BsaHI Gr'CG_yC
Cuts at: 848 1000 2110 2358 3060 4556 848
Size: 152 1110 248 702 1496 971
Fragments arranged by size:
1496 1110 971 702 248 152
BsaWI w'CCGG_w
Cuts at: 613 1658 1868 2389 3067 3478 3625 3899 613
Size: 1045 210 521 678 411 147 274 1393
Fragments arranged by size:
1393 1045 678 521 411 274 210 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 288 318 288
Size: 30 4649
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1354 1405 1621 1354
Size: 51 216 4412
Fragments arranged by size:
4412 216 51
BseYI C'CCAG_C
Cuts at: 423 439 453 647 763 859 1015 1301 2104
Size: 16 14 194 116 96 156 286 803
Cuts at: 2104 2862 3527 3935 423
Size: 758 665 408 1167
Fragments arranged by size:
1167 803 758 665 408 286 194 156
116 96 16 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 1216
Size: 4679
BsiEI CG_ry'CG
Cuts at: 104 2221 2267 3497 104
Size: 2117 46 1230 1286
Fragments arranged by size:
2117 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 490 1286 1478 1693 2471 2661 3521 490
Size: 796 192 215 778 190 860 1648
Fragments arranged by size:
1648 860 796 778 215 192 190
Bsp1286I G_dGCh'C
Cuts at: 141 246 490 511 764 1286 1333 1478 1497
Size: 105 244 21 253 522 47 145 19
Cuts at: 1497 1693 2304 2397 2471 2661 2723 3521 141
Size: 196 611 93 74 190 62 798 1299
Fragments arranged by size:
1299 798 611 522 253 244 196 190
145 105 93 74 62 47 21 19
BspEI T'CCGG_A
Cuts at: 1658 1658
Size: 4679
BspHI T'CATG_A
Cuts at: 3111 3111
Size: 4679
BspMI ACCTGCnnnn'nnnn_
Cuts at: 198 669 697 1331 1382 1729 1943 2245 2626
Size: 471 28 634 51 347 214 302 381
Cuts at: 2626 198
Size: 2251
Fragments arranged by size:
2251 634 471 381 347 302 214 51
28
BsrBI CCG'CTC
Cuts at: 50 113 1042 1152 1361 2971 3025 4509 4674
Size: 63 929 110 209 1610 54 1484 165
Cuts at: 4674 50
Size: 55
Fragments arranged by size:
1610 1484 929 209 165 110 63 55
54
BsrDI GCAATG_nn'
Cuts at: 2591 2591
Size: 4679
BsrFI r'CCGG_y
Cuts at: 277 520 970 1631 2677 2858 277
Size: 243 450 661 1046 181 2098
Fragments arranged by size:
2098 1046 661 450 243 181
BssSI C'ACGA_G
Cuts at: 2950 3658 2950
Size: 708 3971
BstAPI GCAn_nnn'nTGC
Cuts at: 2056 2056
Size: 4679
BstBI TT'CG_AA
Cuts at: 3040 3040
Size: 4679
BstEII G'GTnAC_C
Cuts at: 1611 1611
Size: 4679
BstXI CCAn_nnnn'nTGG
Cuts at: 552 552
Size: 4679
BstYI r'GATC_y
Cuts at: 1 1010 1455 1526 1535 1704 2171 2194 2529
Size: 1009 445 71 9 169 467 23 335
Cuts at: 2529 2775 3081 3093 3179 4231 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 1009 467 449 445 335 306 246
169 86 71 23 12 9
BtgI C'CryG_G
Cuts at: 428 653 428
Size: 225 4454
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2629 2810 2973 2629
Size: 181 163 4335
Fragments arranged by size:
4335 181 163
BtsI GCAGTG_nn'
Cuts at: 164 854 1516 1558 1570 1922 164
Size: 690 662 42 12 352 2921
Fragments arranged by size:
2921 690 662 352 42 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 507 542 957 992 507
Size: 35 415 35 4194
Fragments arranged by size:
4194 415 35 35
DraI TTT'AAA
Cuts at: 989 1678 989
Size: 689 3990
DrdI GACnn_nn'nnGTC
Cuts at: 2385 3729 2385
Size: 1344 3335
EaeI y'GGCC_r
Cuts at: 308 683 2264 2438 2829 2856 4201 308
Size: 375 1581 174 391 27 1345 786
Fragments arranged by size:
1581 1345 786 391 375 174 27
EagI C'GGCC_G
Cuts at: 2264 2264
Size: 4679
EarI CTCTTCn'nnn_
Cuts at: 1261 2702 2912 1261
Size: 1441 210 3028
Fragments arranged by size:
3028 1441 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1074 2887 3629 3775 4489 1074
Size: 1813 742 146 714 1264
Fragments arranged by size:
1813 1264 742 714 146
EcoICRI GAG'CTC
Cuts at: 1284 1691 1284
Size: 407 4272
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 483 542 593 858 911 1126 1147 1153
Size: 238 59 51 265 53 215 21 6
Cuts at: 1153 1357 2504 2936 3289 3951 245
Size: 204 1147 432 353 662 973
Fragments arranged by size:
1147 973 662 432 353 265 238 215
204 59 53 51 21 6
EcoNI CCTnn'n_nnAGG
Cuts at: 194 1165 194
Size: 971 3708
EcoO109I rG'GnC_Cy
Cuts at: 137 138 1493 1494 1818 137
Size: 1 1355 1 324 2998
Fragments arranged by size:
2998 1355 324 1 1
EcoRI G'AATT_C
Cuts at: 639 1683 639
Size: 1044 3635
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 287 417 2698 287
Size: 130 2281 2268
Fragments arranged by size:
2281 2268 130
FspI TGC'GCA
Cuts at: 1547 2460 1547
Size: 913 3766
FspAI rTGC'GCAy
Cuts at: 1547 1547
Size: 4679
HaeII r_GCGC'y
Cuts at: 851 1003 2113 2361 3591 4362 851
Size: 152 1110 248 1230 771 1168
Fragments arranged by size:
1230 1168 1110 771 248 152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 806 838 1205 1237 1564 1596 2521 2551 2553
Size: 32 367 32 327 32 925 30 2
Cuts at: 2553 2583 806
Size: 30 2902
Fragments arranged by size:
2902 925 367 327 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1182 1718 1182
Size: 536 4143
KasI G'GCGC_C
Cuts at: 847 999 2109 2357 847
Size: 152 1110 248 3169
Fragments arranged by size:
3169 1110 248 152
KpnI G_GTAC'C
Cuts at: 1699 1699
Size: 4679
MluI A'CGCG_T
Cuts at: 4112 4112
Size: 4679
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 276 817 3438 3622 276
Size: 541 2621 184 1333
Fragments arranged by size:
2621 1333 541 184
MscI TGG'CCA
Cuts at: 2440 2440
Size: 4679
MslI CAynn'nnrTG
Cuts at: 550 1385 1563 2668 2795 550
Size: 835 178 1105 127 2434
Fragments arranged by size:
2434 1105 835 178 127
MspA1I CmG'CkG
Cuts at: 415 443 724 844 928 1030 1063 1104 1790
Size: 28 281 120 84 102 33 41 686
Cuts at: 1790 2104 2464 3248 3493 415
Size: 314 360 784 245 1601
Fragments arranged by size:
1601 784 686 360 314 281 245 120
102 84 41 33 28
NaeI GCC'GGC
Cuts at: 1633 2860 1633
Size: 1227 3452
NarI GG'CG_CC
Cuts at: 848 1000 2110 2358 848
Size: 152 1110 248 3169
Fragments arranged by size:
3169 1110 248 152
NcoI C'CATG_G
Cuts at: 428 428
Size: 4679
NgoMIV G'CCGG_C
Cuts at: 1631 2858 1631
Size: 1227 3452
NspI r_CATG'y
Cuts at: 1732 2232 3835 1732
Size: 500 1603 2576
Fragments arranged by size:
2576 1603 500
PciI A'CATG_T
Cuts at: 3831 3831
Size: 4679
PmeI GTTT'AAAC
Cuts at: 1678 1678
Size: 4679
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 330 362 3125 3157 330
Size: 32 2763 32 1852
Fragments arranged by size:
2763 1852 32 32
PspOMI G'GGCC_C
Cuts at: 137 760 1493 137
Size: 623 733 3323
Fragments arranged by size:
3323 733 623
PstI C_TGCA'G
Cuts at: 1068 1379 1523 1565 1595 1726 2411 1068
Size: 311 144 42 30 131 685 3336
Fragments arranged by size:
3336 685 311 144 131 42 30
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4679
PvuII CAG'CTG
Cuts at: 415 724 1030 1063 1104 2104 2464 415
Size: 309 306 33 41 1000 360 2630
Fragments arranged by size:
2630 1000 360 309 306 41 33
RsrII CG'GwC_CG
Cuts at: 2874 2874
Size: 4679
SacI G_AGCT'C
Cuts at: 1286 1693 1286
Size: 407 4272
SalI G'TCGA_C
Cuts at: 1716 1716
Size: 4679
SapI GCTCTTCn'nnn_
Cuts at: 2702 2912 2702
Size: 210 4469
SbfI CC_TGCA'GG
Cuts at: 1726 1726
Size: 4679
ScaI AGT'ACT
Cuts at: 756 4196 756
Size: 3440 1239
SfcI C'TryA_G
Cuts at: 33 143 194 1064 1375 1519 1561 1591 1722
Size: 110 51 870 311 144 42 30 131
Cuts at: 1722 2407 3375 3566 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 870 685 311 191 144 131
110 51 42 30
SfoI GGC'GCC
Cuts at: 849 1001 2111 2359 849
Size: 152 1110 248 3169
Fragments arranged by size:
3169 1110 248 152
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4679
SmaI CCC'GGG
Cuts at: 1701 1701
Size: 4679
SmlI C'TyrA_G
Cuts at: 402 1438 1459 1834 2749 3463 3725 402
Size: 1036 21 375 915 714 262 1356
Fragments arranged by size:
1356 1036 915 714 375 262 21
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4679
SphI G_CATG'C
Cuts at: 1732 2232 1732
Size: 500 4179
StyI C'CwwG_G
Cuts at: 428 1616 1813 2167 428
Size: 1188 197 354 2940
Fragments arranged by size:
2940 1188 354 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 313 531 750 1189 2262 2395 2854 3063 313
Size: 218 219 439 1073 133 459 209 1929
Fragments arranged by size:
1929 1073 459 439 219 218 209 133
TatI w'GTAC_w
Cuts at: 754 4194 754
Size: 3440 1239
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 288 320 4196 4228 288
Size: 32 3876 32 739
Fragments arranged by size:
3876 739 32 32
Tth111I GACn'n_nGTC
Cuts at: 2476 2476
Size: 4679
XbaI T'CTAG_A
Cuts at: 1710 1710
Size: 4679
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 426 549 2174 426
Size: 123 1625 2931
Fragments arranged by size:
2931 1625 123
XmaI C'CCGG_G
Cuts at: 1699 1699
Size: 4679
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BglI BmtI Bpu10I BsiWI
BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI DraIII
EcoRV FseI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI