Restriction map for
pF1KB8576
(Circular) (Six-base) MAPSORT of: pF1KB8576.seq Check: 92 from: 1 to: 3671
pF1KB8576 3671 bp
With 182 enzymes: SgfI *
February 20, 2009 16:36 ..
AccI GT'mk_AC
Cuts at: 629 709 629
Size: 80 3591
Acc65I G'GTAC_C
Cuts at: 687 687
Size: 3671
AclI AA'CG_TT
Cuts at: 3511 3511
Size: 3671
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 1496 1928 2281 2943 166
Size: 1330 432 353 662 894
Fragments arranged by size:
1330 894 662 432 353
AflIII A'CryG_T
Cuts at: 314 1023 2823 3104 314
Size: 709 1800 281 881
Fragments arranged by size:
1800 881 709 281
AhdI GACnn_n'nnGTC
Cuts at: 130 130
Size: 3671
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 602 602
Size: 3671
AlwNI CAG_nnn'CTG
Cuts at: 546 976 2414 546
Size: 430 1438 1803
Fragments arranged by size:
1803 1438 430
ApaLI G'TGCA_C
Cuts at: 2509 2509
Size: 3671
ApoI r'AATT_y
Cuts at: 277 473 675 3024 3280 277
Size: 196 202 2349 256 668
Fragments arranged by size:
2349 668 256 202 196
AseI AT'TA_AT
Cuts at: 20 20
Size: 3671
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3671
AvaI C'yCGr_G
Cuts at: 691 691
Size: 3671
BamHI G'GATC_C
Cuts at: 696 696
Size: 3671
BanI G'GyrC_C
Cuts at: 687 1101 1349 1384 687
Size: 414 248 35 2974
Fragments arranged by size:
2974 414 248 35
BanII G_rGCy'C
Cuts at: 685 1715 685
Size: 1030 2641
BbeI G_GCGC'C
Cuts at: 1105 1353 1105
Size: 248 3423
BbsI GAAGACnn'nnnn_
Cuts at: 667 667
Size: 3671
BciVI GTATCCnnnnn_n'
Cuts at: 1569 2625 3671 1569
Size: 1056 1046 1569
Fragments arranged by size:
1569 1056 1046
BclI T'GATC_A
Cuts at: 1191 1191
Size: 3671
BglII A'GATC_T
Cuts at: 1186 3223 1186
Size: 2037 1634
BlpI GC'TnA_GC
Cuts at: 783 783
Size: 3671
Bme1580I G_kGCm'C
Cuts at: 1296 1389 2513 1296
Size: 93 1124 2454
Fragments arranged by size:
2454 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 883 964 1299 3443 883
Size: 81 335 2144 1111
Fragments arranged by size:
2144 1111 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2038 2038
Size: 3671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 1726 2440 2738 847
Size: 879 714 298 1780
Fragments arranged by size:
1780 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3375 3375
Size: 3671
BsaAI yAC'GTr
Cuts at: 1024 1024
Size: 3671
BsaBI GATnn'nnATC
Cuts at: 6 1185 3222 6
Size: 1179 2037 455
Fragments arranged by size:
2037 1179 455
BsaHI Gr'CG_yC
Cuts at: 1102 1350 2052 3548 1102
Size: 248 702 1496 1225
Fragments arranged by size:
1496 1225 702 248
BsaWI w'CCGG_w
Cuts at: 860 1381 2059 2470 2617 2891 860
Size: 521 678 411 147 274 1640
Fragments arranged by size:
1640 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1096 1854 2519 2927 1096
Size: 758 665 408 1840
Fragments arranged by size:
1840 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1213 1259 2489 104
Size: 1109 46 1230 1286
Fragments arranged by size:
1286 1230 1109 46
BsiHKAI G_wGCw'C
Cuts at: 685 1463 1653 2513 685
Size: 778 190 860 1843
Fragments arranged by size:
1843 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 685 1296 1389 1463 1653 1715 2513 685
Size: 611 93 74 190 62 798 1843
Fragments arranged by size:
1843 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2103 2103
Size: 3671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 721 935 1237 1618 721
Size: 214 302 381 2774
Fragments arranged by size:
2774 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1963 2017 3501 3666 50
Size: 1913 54 1484 165 55
Fragments arranged by size:
1913 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1583 1583
Size: 3671
BsrFI r'CCGG_y
Cuts at: 1669 1850 1669
Size: 181 3490
BssSI C'ACGA_G
Cuts at: 1942 2650 1942
Size: 708 2963
BstAPI GCAn_nnn'nTGC
Cuts at: 1048 1048
Size: 3671
BstBI TT'CG_AA
Cuts at: 2032 2032
Size: 3671
BstYI r'GATC_y
Cuts at: 1 504 647 696 1163 1186 1521 1767 2073
Size: 503 143 49 467 23 335 246 306
Cuts at: 2073 2085 2171 3223 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 503 467 449 335 306 246 143
86 49 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3671
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1621 1802 1965 1621
Size: 181 163 3327
Fragments arranged by size:
3327 181 163
BtsI GCAGTG_nn'
Cuts at: 524 914 524
Size: 390 3281
DraI TTT'AAA
Cuts at: 670 670
Size: 3671
DrdI GACnn_nn'nnGTC
Cuts at: 1377 2721 1377
Size: 1344 2327
EaeI y'GGCC_r
Cuts at: 419 1256 1430 1821 1848 3193 419
Size: 837 174 391 27 1345 897
Fragments arranged by size:
1345 897 837 391 174 27
EagI C'GGCC_G
Cuts at: 1256 1256
Size: 3671
EarI CTCTTCn'nnn_
Cuts at: 446 1694 1904 446
Size: 1248 210 2213
Fragments arranged by size:
2213 1248 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1879 2621 2767 3481 1879
Size: 742 146 714 2069
Fragments arranged by size:
2069 742 714 146
EcoICRI GAG'CTC
Cuts at: 683 683
Size: 3671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 1496 1928 2281 2943 166
Size: 1330 432 353 662 894
Fragments arranged by size:
1330 894 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 810 810
Size: 3671
EcoRI G'AATT_C
Cuts at: 473 675 473
Size: 202 3469
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1690 1690
Size: 3671
FspI TGC'GCA
Cuts at: 1452 1452
Size: 3671
HaeII r_GCGC'y
Cuts at: 1105 1353 2583 3354 1105
Size: 248 1230 771 1422
Fragments arranged by size:
1422 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 116 148 1513 1543 1545 1575 116
Size: 32 1365 30 2 30 2212
Fragments arranged by size:
2212 1365 32 30 30 2
HincII GTy'rAC
Cuts at: 710 710
Size: 3671
KasI G'GCGC_C
Cuts at: 1101 1349 1101
Size: 248 3423
KpnI G_GTAC'C
Cuts at: 691 691
Size: 3671
MluI A'CGCG_T
Cuts at: 3104 3104
Size: 3671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 277 2430 2614 277
Size: 2153 184 1334
Fragments arranged by size:
2153 1334 184
MscI TGG'CCA
Cuts at: 421 1432 421
Size: 1011 2660
MslI CAynn'nnrTG
Cuts at: 584 1660 1787 584
Size: 1076 127 2468
Fragments arranged by size:
2468 1076 127
MspA1I CmG'CkG
Cuts at: 782 1096 1456 2240 2485 782
Size: 314 360 784 245 1968
Fragments arranged by size:
1968 784 360 314 245
NaeI GCC'GGC
Cuts at: 1852 1852
Size: 3671
NarI GG'CG_CC
Cuts at: 1102 1350 1102
Size: 248 3423
NcoI C'CATG_G
Cuts at: 107 107
Size: 3671
NgoMIV G'CCGG_C
Cuts at: 1850 1850
Size: 3671
NspI r_CATG'y
Cuts at: 318 724 1224 2827 318
Size: 406 500 1603 1162
Fragments arranged by size:
1603 1162 500 406
PciI A'CATG_T
Cuts at: 314 2823 314
Size: 2509 1162
PmeI GTTT'AAAC
Cuts at: 670 670
Size: 3671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2117 2149 2117
Size: 32 3639
PsiI TTA'TAA
Cuts at: 359 359
Size: 3671
PstI C_TGCA'G
Cuts at: 718 1403 718
Size: 685 2986
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3671
PvuII CAG'CTG
Cuts at: 1096 1456 1096
Size: 360 3311
RsrII CG'GwC_CG
Cuts at: 1866 1866
Size: 3671
SacI G_AGCT'C
Cuts at: 685 685
Size: 3671
SalI G'TCGA_C
Cuts at: 708 708
Size: 3671
SapI GCTCTTCn'nnn_
Cuts at: 1694 1904 1694
Size: 210 3461
SbfI CC_TGCA'GG
Cuts at: 718 718
Size: 3671
ScaI AGT'ACT
Cuts at: 3188 3188
Size: 3671
SfcI C'TryA_G
Cuts at: 33 714 1399 2367 2558 33
Size: 681 685 968 191 1146
Fragments arranged by size:
1146 968 685 681 191
SfoI GGC'GCC
Cuts at: 1103 1351 1103
Size: 248 3423
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3671
SmaI CCC'GGG
Cuts at: 693 693
Size: 3671
SmlI C'TyrA_G
Cuts at: 826 1741 2455 2717 826
Size: 915 714 262 1780
Fragments arranged by size:
1780 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3671
SphI G_CATG'C
Cuts at: 724 1224 724
Size: 500 3171
StyI C'CwwG_G
Cuts at: 107 174 805 1159 107
Size: 67 631 354 2619
Fragments arranged by size:
2619 631 354 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1254 1387 1846 2055 1254
Size: 133 459 209 2870
Fragments arranged by size:
2870 459 209 133
TatI w'GTAC_w
Cuts at: 3186 3186
Size: 3671
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 3639
Tth111I GACn'n_nGTC
Cuts at: 1468 1468
Size: 3671
XbaI T'CTAG_A
Cuts at: 702 702
Size: 3671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1166 1166
Size: 3671
XmaI C'CCGG_G
Cuts at: 691 691
Size: 3671
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI