Restriction map for
pF1KB8545
(Circular) (Six-base) MAPSORT of: pF1KB8545.seq Check: 5352 from: 1 to: 6065
pF1KB8545 6065 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AccI GT'mk_AC
Cuts at: 353 1953 2204 3103 353
Size: 1600 251 899 3315
Fragments arranged by size:
3315 1600 899 251
Acc65I G'GTAC_C
Cuts at: 3081 3081
Size: 6065
AclI AA'CG_TT
Cuts at: 5905 5905
Size: 6065
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 1692 3890 4322 5069 5337 676
Size: 1016 2198 432 747 268 1404
Fragments arranged by size:
2198 1404 1016 747 432 268
AfeI AGC'GCT
Cuts at: 2874 2874
Size: 6065
AflIII A'CryG_T
Cuts at: 3417 4521 5498 3417
Size: 1104 977 3984
Fragments arranged by size:
3984 1104 977
AgeI A'CCGG_T
Cuts at: 4453 4453
Size: 6065
AlwNI CAG_nnn'CTG
Cuts at: 642 1921 3370 4937 642
Size: 1279 1449 1567 1770
Fragments arranged by size:
1770 1567 1449 1279
ApaLI G'TGCA_C
Cuts at: 4835 4835
Size: 6065
ApoI r'AATT_y
Cuts at: 1403 1447 1589 2228 2572 2953 3069 5418 5674
Size: 44 142 639 344 381 116 2349 256
Cuts at: 5674 1403
Size: 1794
Fragments arranged by size:
2349 1794 639 381 344 256 142 116
44
AseI AT'TA_AT
Cuts at: 20 1643 20
Size: 1623 4442
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6065
AvaI C'yCGr_G
Cuts at: 2192 2987 3085 2192
Size: 795 98 5172
Fragments arranged by size:
5172 795 98
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 212 245 574 607 212
Size: 33 329 33 5670
Fragments arranged by size:
5670 329 33 33
BamHI G'GATC_C
Cuts at: 3090 3090
Size: 6065
BanI G'GyrC_C
Cuts at: 1702 2801 3081 3495 3743 3778 1702
Size: 1099 280 414 248 35 3989
Fragments arranged by size:
3989 1099 414 280 248 35
BanII G_rGCy'C
Cuts at: 1629 3079 4109 1629
Size: 1450 1030 3585
Fragments arranged by size:
3585 1450 1030
BbeI G_GCGC'C
Cuts at: 2805 3499 3747 2805
Size: 694 248 5123
Fragments arranged by size:
5123 694 248
BbsI GAAGACnn'nnnn_
Cuts at: 163 1569 163
Size: 1406 4659
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2806 2840 2806
Size: 34 6031
BciVI GTATCCnnnnn_n'
Cuts at: 2744 2993 3963 4724 6065 2744
Size: 249 970 761 1341 2744
Fragments arranged by size:
2744 1341 970 761 249
BclI T'GATC_A
Cuts at: 3585 3585
Size: 6065
BglII A'GATC_T
Cuts at: 2041 3580 5617 2041
Size: 1539 2037 2489
Fragments arranged by size:
2489 2037 1539
BlpI GC'TnA_GC
Cuts at: 635 3177 635
Size: 2542 3523
Bme1580I G_kGCm'C
Cuts at: 3690 3783 4839 3690
Size: 93 1056 4916
Fragments arranged by size:
4916 1056 93
BmgBI CAC'GTC
Cuts at: 1030 1030
Size: 6065
BmrI ACTGGGnnnn_n'
Cuts at: 3277 3358 3693 5837 3277
Size: 81 335 2144 3505
Fragments arranged by size:
3505 2144 335 81
BmtI G_CTAG'C
Cuts at: 1784 2816 1784
Size: 1032 5033
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4432 4432
Size: 6065
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 455 631 1760 1817 455
Size: 176 1129 57 4703
Fragments arranged by size:
4703 1129 176 57
Bpu10I CC'TnA_GC
Cuts at: 840 840
Size: 6065
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 863 1700 1837 2050 3241 4120 4612 4910 863
Size: 837 137 213 1191 879 492 298 2018
Fragments arranged by size:
2018 1191 879 837 492 298 213 137
BsaI GGTCTCn'nnnn_
Cuts at: 1436 5769 1436
Size: 4333 1732
BsaAI yAC'GTr
Cuts at: 1393 3418 1393
Size: 2025 4040
BsaBI GATnn'nnATC
Cuts at: 6 3579 5616 6
Size: 3573 2037 455
Fragments arranged by size:
3573 2037 455
BsaHI Gr'CG_yC
Cuts at: 112 2802 3496 3744 4446 5942 112
Size: 2690 694 248 702 1496 235
Fragments arranged by size:
2690 1496 702 694 248 235
BsaWI w'CCGG_w
Cuts at: 3254 3775 4453 4727 4874 5285 3254
Size: 521 678 274 147 411 4034
Fragments arranged by size:
4034 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1176 1206 2171 2201 1176
Size: 30 965 30 5040
Fragments arranged by size:
5040 965 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1619 1820 1982 3028 1619
Size: 201 162 1046 4656
Fragments arranged by size:
4656 1046 201 162
BseYI C'CCAG_C
Cuts at: 3490 4248 4825 5321 3490
Size: 758 577 496 4234
Fragments arranged by size:
4234 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 590 250
Size: 340 5725
BsiEI CG_ry'CG
Cuts at: 104 3607 3653 4861 104
Size: 3503 46 1208 1308
Fragments arranged by size:
3503 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1629 3079 3857 4047 4839 1629
Size: 1450 778 190 792 2855
Fragments arranged by size:
2855 1450 792 778 190
BsmI GAATG_Cn'
Cuts at: 686 686
Size: 6065
Bsp1286I G_dGCh'C
Cuts at: 1629 3079 3690 3783 3857 4047 4109 4839 1629
Size: 1450 611 93 74 190 62 730 2855
Fragments arranged by size:
2855 1450 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 5285 5285
Size: 6065
BspHI T'CATG_A
Cuts at: 1211 5241 1211
Size: 4030 2035
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3115 3329 3631 4012 3115
Size: 214 302 381 5168
Fragments arranged by size:
5168 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4357 4411 5895 6060 50
Size: 4307 54 1484 165 55
Fragments arranged by size:
4307 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3044 3977 3044
Size: 933 5132
BsrFI r'CCGG_y
Cuts at: 2329 4063 4244 4453 2329
Size: 1734 181 209 3941
Fragments arranged by size:
3941 1734 209 181
BssSI C'ACGA_G
Cuts at: 1844 4336 4694 1844
Size: 2492 358 3215
Fragments arranged by size:
3215 2492 358
BstAPI GCAn_nnn'nTGC
Cuts at: 811 3442 811
Size: 2631 3434
BstBI TT'CG_AA
Cuts at: 4426 4426
Size: 6065
BstEII G'GTnAC_C
Cuts at: 1533 1533
Size: 6065
BstXI CCAn_nnnn'nTGG
Cuts at: 1486 1486
Size: 6065
BstYI r'GATC_y
Cuts at: 1 438 601 2041 3090 3557 3580 3915 4161
Size: 437 163 1440 1049 467 23 335 246
Cuts at: 4161 5173 5259 5271 5617 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1440 1049 1012 467 449 437 346 335
246 163 86 23 12
BstZ17I GTA'TAC
Cuts at: 1954 1954
Size: 6065
BtgI C'CryG_G
Cuts at: 107 624 107
Size: 517 5548
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4015 4196 4359 4015
Size: 181 163 5721
Fragments arranged by size:
5721 181 163
BtsI GCAGTG_nn'
Cuts at: 1171 3308 1171
Size: 2137 3928
DraI TTT'AAA
Cuts at: 920 3064 920
Size: 2144 3921
DrdI GACnn_nn'nnGTC
Cuts at: 3771 4629 3771
Size: 858 5207
EaeI y'GGCC_r
Cuts at: 1320 3650 3824 4215 4242 5587 1320
Size: 2330 174 391 27 1345 1798
Fragments arranged by size:
2330 1798 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3650 3650
Size: 6065
EarI CTCTTCn'nnn_
Cuts at: 1668 2094 2687 3005 4088 4298 1668
Size: 426 593 318 1083 210 3435
Fragments arranged by size:
3435 1083 593 426 318 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4273 4583 4729 5875 4273
Size: 310 146 1146 4463
Fragments arranged by size:
4463 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1627 3077 1627
Size: 1450 4615
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 455 631 676 1692 1760 1817 3890 4322 5069
Size: 176 45 1016 68 57 2073 432 747
Cuts at: 5069 5337 455
Size: 268 1183
Fragments arranged by size:
2073 1183 1016 747 432 268 176 68
57 45
EcoNI CCTnn'n_nnAGG
Cuts at: 1997 1997
Size: 6065
EcoO109I rG'GnC_Cy
Cuts at: 525 2337 2631 3204 525
Size: 1812 294 573 3386
Fragments arranged by size:
3386 1812 573 294
EcoRI G'AATT_C
Cuts at: 3069 3069
Size: 6065
EcoRV GAT'ATC
Cuts at: 249 1043 1128 249
Size: 794 85 5186
Fragments arranged by size:
5186 794 85
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 362 1126 4084 362
Size: 764 2958 2343
Fragments arranged by size:
2958 2343 764
FspI TGC'GCA
Cuts at: 3846 3846
Size: 6065
HaeII r_GCGC'y
Cuts at: 2805 2876 3499 3747 4769 5748 2805
Size: 71 623 248 1022 979 3122
Fragments arranged by size:
3122 1022 979 623 248 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 732 764 1258 1290 1414 1446 2026 2058 2970
Size: 32 494 32 124 32 580 32 912
Cuts at: 2970 3002 3907 3937 3939 3969 732
Size: 32 905 30 2 30 2828
Fragments arranged by size:
2828 912 905 580 494 124 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3104 3104
Size: 6065
KasI G'GCGC_C
Cuts at: 2801 3495 3743 2801
Size: 694 248 5123
Fragments arranged by size:
5123 694 248
KpnI G_GTAC'C
Cuts at: 3085 3085
Size: 6065
MfeI C'AATT_G
Cuts at: 538 538
Size: 6065
MluI A'CGCG_T
Cuts at: 5498 5498
Size: 6065
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 140 890 1162 4736 4920 140
Size: 750 272 3574 184 1285
Fragments arranged by size:
3574 1285 750 272 184
MscI TGG'CCA
Cuts at: 1322 3826 1322
Size: 2504 3561
MslI CAynn'nnrTG
Cuts at: 679 2360 4054 4181 679
Size: 1681 1694 127 2563
Fragments arranged by size:
2563 1694 1681 127
MspA1I CmG'CkG
Cuts at: 3176 3490 3850 4863 5108 3176
Size: 314 360 1013 245 4133
Fragments arranged by size:
4133 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4246 4246
Size: 6065
NarI GG'CG_CC
Cuts at: 2802 3496 3744 2802
Size: 694 248 5123
Fragments arranged by size:
5123 694 248
NcoI C'CATG_G
Cuts at: 107 624 107
Size: 517 5548
NgoMIV G'CCGG_C
Cuts at: 4244 4244
Size: 6065
NheI G'CTAG_C
Cuts at: 1780 2812 1780
Size: 1032 5033
NspI r_CATG'y
Cuts at: 1494 2285 2305 3118 3618 4525 1494
Size: 791 20 813 500 907 3034
Fragments arranged by size:
3034 907 813 791 500 20
PciI A'CATG_T
Cuts at: 4521 4521
Size: 6065
PfoI T'CCnGG_A
Cuts at: 647 647
Size: 6065
PmeI GTTT'AAAC
Cuts at: 3064 3064
Size: 6065
PmlI CAC'GTG
Cuts at: 1393 1393
Size: 6065
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5204 5236 5204
Size: 32 6033
PpuMI rG'GwC_Cy
Cuts at: 2337 2337
Size: 6065
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2725 2757 2984 3016 2725
Size: 32 227 32 5774
Fragments arranged by size:
5774 227 32 32
PstI C_TGCA'G
Cuts at: 155 410 3112 3797 155
Size: 255 2702 685 2423
Fragments arranged by size:
2702 2423 685 255
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6065
PvuII CAG'CTG
Cuts at: 3490 3850 3490
Size: 360 5705
RsrII CG'GwC_CG
Cuts at: 4260 4260
Size: 6065
SacI G_AGCT'C
Cuts at: 1629 3079 1629
Size: 1450 4615
SalI G'TCGA_C
Cuts at: 3102 3102
Size: 6065
SapI GCTCTTCn'nnn_
Cuts at: 1668 4088 4298 1668
Size: 2420 210 3435
Fragments arranged by size:
3435 2420 210
SbfI CC_TGCA'GG
Cuts at: 3112 3112
Size: 6065
ScaI AGT'ACT
Cuts at: 5582 5582
Size: 6065
SfcI C'TryA_G
Cuts at: 33 151 406 1685 1733 1790 2618 2750 3108
Size: 118 255 1279 48 57 828 132 358
Cuts at: 3108 3793 4786 4977 33
Size: 685 993 191 1121
Fragments arranged by size:
1279 1121 993 828 685 358 255 191
132 118 57 48
SfoI GGC'GCC
Cuts at: 2803 3497 3745 2803
Size: 694 248 5123
Fragments arranged by size:
5123 694 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6065
SmaI CCC'GGG
Cuts at: 3087 3087
Size: 6065
SmlI C'TyrA_G
Cuts at: 878 1715 1816 2029 2192 2987 3220 4135 4627
Size: 837 101 213 163 795 233 915 492
Cuts at: 4627 4889 878
Size: 262 2054
Fragments arranged by size:
2054 915 837 795 492 262 233 213
163 101
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6065
SphI G_CATG'C
Cuts at: 3118 3618 3118
Size: 500 5565
SspI AAT'ATT
Cuts at: 1641 1647 1641
Size: 6 6059
StuI AGG'CCT
Cuts at: 2003 2003
Size: 6065
StyI C'CwwG_G
Cuts at: 107 463 624 2634 2737 2805 2995 3199 3553
Size: 356 161 2010 103 68 190 204 354
Cuts at: 3553 107
Size: 2619
Fragments arranged by size:
2619 2010 356 354 204 190 161 103
68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2861 3648 3781 4240 4449 2861
Size: 787 133 459 209 4477
Fragments arranged by size:
4477 787 459 209 133
TatI w'GTAC_w
Cuts at: 1063 5580 1063
Size: 4517 1548
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1176 1208 2171 2203 5582 5614 1176
Size: 32 963 32 3379 32 1627
Fragments arranged by size:
3379 1627 963 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3862 3862
Size: 6065
XbaI T'CTAG_A
Cuts at: 3096 3096
Size: 6065
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3560 3560
Size: 6065
XhoI C'TCGA_G
Cuts at: 2192 2987 2192
Size: 795 5270
XmaI C'CCGG_G
Cuts at: 3085 3085
Size: 6065
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PfoI PmeI PmlI
PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BbvCI BfrBI BglI BsiWI BsmBI BsrGI
BssHII Bsu36I ClaI CspCI DraIII FseI FspAI HindIII
HpaI NdeI NotI NruI NsiI PacI PasI PflMI
PshAI PsiI PspOMI PspXI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XmnI ZraI