Restriction map for
pF1KB8539
(Circular) (Six-base) MAPSORT of: pF1KB8539.seq Check: 9374 from: 1 to: 5978
pF1KB8539 5978 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 698 2475 2493 2502 2844 698
Size: 1777 18 9 342 3832
Fragments arranged by size:
3832 1777 342 18 9
AatII G_ACGT'C
Cuts at: 1256 1532 1889 2162 1256
Size: 276 357 273 5072
Fragments arranged by size:
5072 357 276 273
AccI GT'mk_AC
Cuts at: 2062 3016 2062
Size: 954 5024
Acc65I G'GTAC_C
Cuts at: 1340 1493 2994 1340
Size: 153 1501 4324
Fragments arranged by size:
4324 1501 153
AclI AA'CG_TT
Cuts at: 5818 5818
Size: 5978
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1405 2626 3803 4235 4982 5250 1405
Size: 1221 1177 432 747 268 2133
Fragments arranged by size:
2133 1221 1177 747 432 268
AfeI AGC'GCT
Cuts at: 2674 2674
Size: 5978
AflIII A'CryG_T
Cuts at: 3330 4434 5411 3330
Size: 1104 977 3897
Fragments arranged by size:
3897 1104 977
AgeI A'CCGG_T
Cuts at: 4366 4366
Size: 5978
AleI CACnn'nnGTG
Cuts at: 710 2948 710
Size: 2238 3740
AlwNI CAG_nnn'CTG
Cuts at: 1119 2230 2322 2358 3283 4850 1119
Size: 1111 92 36 925 1567 2247
Fragments arranged by size:
2247 1567 1111 925 92 36
ApaI G_GGCC'C
Cuts at: 2198 2198
Size: 5978
ApaLI G'TGCA_C
Cuts at: 1167 4748 1167
Size: 3581 2397
ApoI r'AATT_y
Cuts at: 2409 2982 5331 5587 2409
Size: 573 2349 256 2800
Fragments arranged by size:
2800 2349 573 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5978
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5978
AvaI C'yCGr_G
Cuts at: 1765 1924 2203 2433 2998 1765
Size: 159 279 230 565 4745
Fragments arranged by size:
4745 565 279 230 159
BamHI G'GATC_C
Cuts at: 3003 3003
Size: 5978
BanI G'GyrC_C
Cuts at: 654 1340 1493 2584 2994 3408 3656 3691 654
Size: 686 153 1091 410 414 248 35 2941
Fragments arranged by size:
2941 1091 686 414 410 248 153 35
BanII G_rGCy'C
Cuts at: 146 989 1079 2045 2198 2882 2933 2992 4022
Size: 843 90 966 153 684 51 59 1030
Cuts at: 4022 146
Size: 2102
Fragments arranged by size:
2102 1030 966 843 684 153 90 59
51
BbeI G_GCGC'C
Cuts at: 2588 3412 3660 2588
Size: 824 248 4906
Fragments arranged by size:
4906 824 248
BbsI GAAGACnn'nnnn_
Cuts at: 2154 2154
Size: 5978
BbvCI CC'TCA_GC
Cuts at: 1864 2218 1864
Size: 354 5624
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 529 563 90
Size: 34 405 34 5505
Fragments arranged by size:
5505 405 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3876 4637 5978 3876
Size: 761 1341 3876
Fragments arranged by size:
3876 1341 761
BclI T'GATC_A
Cuts at: 3498 3498
Size: 5978
BglI GCCn_nnn'nGGC
Cuts at: 112 1399 1720 2593 112
Size: 1287 321 873 3497
Fragments arranged by size:
3497 1287 873 321
BglII A'GATC_T
Cuts at: 3493 5530 3493
Size: 2037 3941
BlpI GC'TnA_GC
Cuts at: 2686 3090 2686
Size: 404 5574
Bme1580I G_kGCm'C
Cuts at: 335 659 1171 2198 3603 3696 4752 335
Size: 324 512 1027 1405 93 1056 1561
Fragments arranged by size:
1561 1405 1056 1027 512 324 93
BmgBI CAC'GTC
Cuts at: 779 1827 779
Size: 1048 4930
BmrI ACTGGGnnnn_n'
Cuts at: 1660 2608 3190 3271 3606 5750 1660
Size: 948 582 81 335 2144 1888
Fragments arranged by size:
2144 1888 948 582 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2711 2952 4345 2711
Size: 241 1393 4344
Fragments arranged by size:
4344 1393 241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 823 902 979 1177 1366 1552 1641 1924 2428
Size: 79 77 198 189 186 89 283 504
Cuts at: 2428 2896 823
Size: 468 3905
Fragments arranged by size:
3905 504 468 283 198 189 186 89
79 77
Bpu10I CC'TnA_GC
Cuts at: 805 1864 2218 805
Size: 1059 354 4565
Fragments arranged by size:
4565 1059 354
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1222 3154 4033 4525 4823 1222
Size: 1932 879 492 298 2377
Fragments arranged by size:
2377 1932 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1036 1126 1762 2432 5682 1036
Size: 90 636 670 3250 1332
Fragments arranged by size:
3250 1332 670 636 90
BsaAI yAC'GTr
Cuts at: 1434 1476 3331 1434
Size: 42 1855 4081
Fragments arranged by size:
4081 1855 42
BsaBI GATnn'nnATC
Cuts at: 6 3492 5529 6
Size: 3486 2037 455
Fragments arranged by size:
3486 2037 455
BsaHI Gr'CG_yC
Cuts at: 1253 1529 1790 1886 2065 2159 2548 2585 3409
Size: 276 261 96 179 94 389 37 824
Cuts at: 3409 3657 4359 5855 1253
Size: 248 702 1496 1376
Fragments arranged by size:
1496 1376 824 702 389 276 261 248
179 96 94 37
BsaWI w'CCGG_w
Cuts at: 167 1301 2253 2573 3167 3688 4366 4640 4787
Size: 1134 952 320 594 521 678 274 147
Cuts at: 4787 5198 167
Size: 411 947
Fragments arranged by size:
1134 952 947 678 594 521 411 320
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 214 244 1150 1180 1477 1507 1639 1669 214
Size: 30 906 30 297 30 132 30 4523
Fragments arranged by size:
4523 906 297 132 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 810 1273 1387 1498 1921 2221 810
Size: 463 114 111 423 300 4567
Fragments arranged by size:
4567 463 423 300 114 111
BseYI C'CCAG_C
Cuts at: 864 1107 1642 1808 2011 2050 2180 2185 3403
Size: 243 535 166 203 39 130 5 1218
Cuts at: 3403 4161 4738 5234 864
Size: 758 577 496 1608
Fragments arranged by size:
1608 1218 758 577 535 496 243 203
166 130 39 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 1027 1690 1725 2419 2482 2527 487
Size: 540 663 35 694 63 45 3938
Fragments arranged by size:
3938 694 663 540 63 45 35
BsiEI CG_ry'CG
Cuts at: 104 116 725 2177 3520 3566 4774 104
Size: 12 609 1452 1343 46 1208 1308
Fragments arranged by size:
1452 1343 1308 1208 609 46 12
BsiHKAI G_wGCw'C
Cuts at: 971 1171 2519 2992 3770 3960 4752 971
Size: 200 1348 473 778 190 792 2197
Fragments arranged by size:
2197 1348 792 778 473 200 190
BsmI GAATG_Cn'
Cuts at: 1011 1011
Size: 5978
BsmBI CGTCTCn'nnnn_
Cuts at: 789 822 1342 2940 789
Size: 33 520 1598 3827
Fragments arranged by size:
3827 1598 520 33
Bsp1286I G_dGCh'C
Cuts at: 146 335 659 971 989 1079 1171 2045 2198
Size: 189 324 312 18 90 92 874 153
Cuts at: 2198 2519 2882 2933 2992 3603 3696 3770 3960
Size: 321 363 51 59 611 93 74 190
Cuts at: 3960 4022 4752 146
Size: 62 730 1372
Fragments arranged by size:
1372 874 730 611 363 324 321 312
190 189 153 93 92 90 74 62
59 51 18
BspEI T'CCGG_A
Cuts at: 167 2253 5198 167
Size: 2086 2945 947
Fragments arranged by size:
2945 2086 947
BspHI T'CATG_A
Cuts at: 5154 5154
Size: 5978
BspMI ACCTGCnnnn'nnnn_
Cuts at: 698 1148 2475 2493 2502 2844 3028 3242 3544
Size: 450 1327 18 9 342 184 214 302
Cuts at: 3544 3925 698
Size: 381 2751
Fragments arranged by size:
2751 1327 450 381 342 302 214 184
18 9
BsrBI CCG'CTC
Cuts at: 50 230 1248 1655 4270 4324 5808 5973 50
Size: 180 1018 407 2615 54 1484 165 55
Fragments arranged by size:
2615 1484 1018 407 180 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1314 1460 1824 3890 1314
Size: 146 364 2066 3402
Fragments arranged by size:
3402 2066 364 146
BsrFI r'CCGG_y
Cuts at: 111 1801 1978 2903 3976 4157 4366 111
Size: 1690 177 925 1073 181 209 1723
Fragments arranged by size:
1723 1690 1073 925 209 181 177
BsrGI T'GTAC_A
Cuts at: 632 632
Size: 5978
BssHII G'CGCG_C
Cuts at: 2498 2498
Size: 5978
BssSI C'ACGA_G
Cuts at: 1585 4249 4607 1585
Size: 2664 358 2956
Fragments arranged by size:
2956 2664 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2514 3355 2514
Size: 841 5137
BstBI TT'CG_AA
Cuts at: 4339 4339
Size: 5978
BstEII G'GTnAC_C
Cuts at: 388 388
Size: 5978
BstXI CCAn_nnnn'nTGG
Cuts at: 872 1086 1902 2475 2649 2766 872
Size: 214 816 573 174 117 4084
Fragments arranged by size:
4084 816 573 214 174 117
BstYI r'GATC_y
Cuts at: 1 3003 3470 3493 3828 4074 5086 5172 5184
Size: 3002 467 23 335 246 1012 86 12
Cuts at: 5184 5530 1
Size: 346 449
Fragments arranged by size:
3002 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 2231 2231
Size: 5978
BtgI C'CryG_G
Cuts at: 659 1415 1847 1895 2735 659
Size: 756 432 48 840 3902
Fragments arranged by size:
3902 840 756 432 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 143 495 3928 4109 4272 143
Size: 352 3433 181 163 1849
Fragments arranged by size:
3433 1849 352 181 163
BtsI GCAGTG_nn'
Cuts at: 504 910 3221 504
Size: 406 2311 3261
Fragments arranged by size:
3261 2311 406
DraI TTT'AAA
Cuts at: 2977 2977
Size: 5978
DrdI GACnn_nn'nnGTC
Cuts at: 3684 4542 3684
Size: 858 5120
EaeI y'GGCC_r
Cuts at: 113 218 312 467 575 722 1175 1391 1400
Size: 105 94 155 108 147 453 216 9
Cuts at: 1400 1712 1874 1892 1946 1998 2174 3563 3737
Size: 312 162 18 54 52 176 1389 174
Cuts at: 3737 4128 4155 5500 113
Size: 391 27 1345 591
Fragments arranged by size:
1389 1345 591 453 391 312 216 176
174 162 155 147 108 105 94 54
52 27 18 9
EagI C'GGCC_G
Cuts at: 113 722 2174 3563 113
Size: 609 1452 1389 2528
Fragments arranged by size:
2528 1452 1389 609
EarI CTCTTCn'nnn_
Cuts at: 1042 1765 2380 4001 4211 1042
Size: 723 615 1621 210 2809
Fragments arranged by size:
2809 1621 723 615 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 205 424 2067 4186 4496 4642 5788 205
Size: 219 1643 2119 310 146 1146 395
Fragments arranged by size:
2119 1643 1146 395 310 219 146
EcoICRI GAG'CTC
Cuts at: 2990 2990
Size: 5978
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 823 902 979 1177 1366 1405 1552 1641 1924
Size: 79 77 198 189 39 147 89 283
Cuts at: 1924 2428 2626 2896 3803 4235 4982 5250 823
Size: 504 198 270 907 432 747 268 1551
Fragments arranged by size:
1551 907 747 504 432 283 270 268
198 198 189 147 89 79 77 39
EcoO109I rG'GnC_Cy
Cuts at: 1572 1647 2007 2194 2227 2301 2595 3117 1572
Size: 75 360 187 33 74 294 522 4433
Fragments arranged by size:
4433 522 360 294 187 75 74 33
EcoRI G'AATT_C
Cuts at: 2982 2982
Size: 5978
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1572 3997 1572
Size: 2425 3553
FspI TGC'GCA
Cuts at: 1816 2917 3759 1816
Size: 1101 842 4035
Fragments arranged by size:
4035 1101 842
HaeII r_GCGC'y
Cuts at: 1567 1726 1988 2588 2676 3412 3660 4682 5661
Size: 159 262 600 88 736 248 1022 979
Cuts at: 5661 1567
Size: 1884
Fragments arranged by size:
1884 1022 979 736 600 262 248 159
88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1639 1671 1918 1950 3820 3850 3852 3882 1639
Size: 32 247 32 1870 30 2 30 3735
Fragments arranged by size:
3735 1870 247 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2063 3017 2063
Size: 954 5024
HindIII A'AGCT_T
Cuts at: 2761 2761
Size: 5978
KasI G'GCGC_C
Cuts at: 2584 3408 3656 2584
Size: 824 248 4906
Fragments arranged by size:
4906 824 248
KpnI G_GTAC'C
Cuts at: 1344 1497 2998 1344
Size: 153 1501 4324
Fragments arranged by size:
4324 1501 153
MluI A'CGCG_T
Cuts at: 5411 5411
Size: 5978
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 185 1040 1307 2373 4649 4833 185
Size: 855 267 1066 2276 184 1330
Fragments arranged by size:
2276 1330 1066 855 267 184
MscI TGG'CCA
Cuts at: 220 314 469 1177 1393 1714 1876 1894 1948
Size: 94 155 708 216 321 162 18 54
Cuts at: 1948 3739 220
Size: 1791 2459
Fragments arranged by size:
2459 1791 708 321 216 162 155 94
54 18
MslI CAynn'nnrTG
Cuts at: 403 487 571 710 712 2647 2948 3967 4094
Size: 84 84 139 2 1935 301 1019 127
Cuts at: 4094 403
Size: 2287
Fragments arranged by size:
2287 1935 1019 301 139 127 84 84
2
MspA1I CmG'CkG
Cuts at: 553 1107 1269 1427 1812 2319 2454 2556 2685
Size: 554 162 158 385 507 135 102 129
Cuts at: 2685 3089 3403 3763 4776 5021 553
Size: 404 314 360 1013 245 1510
Fragments arranged by size:
1510 1013 554 507 404 385 360 314
245 162 158 135 129 102
NaeI GCC'GGC
Cuts at: 113 1803 1980 4159 113
Size: 1690 177 2179 1932
Fragments arranged by size:
2179 1932 1690 177
NarI GG'CG_CC
Cuts at: 2585 3409 3657 2585
Size: 824 248 4906
Fragments arranged by size:
4906 824 248
NcoI C'CATG_G
Cuts at: 659 2735 659
Size: 2076 3902
NgoMIV G'CCGG_C
Cuts at: 111 1801 1978 4157 111
Size: 1690 177 2179 1932
Fragments arranged by size:
2179 1932 1690 177
NspI r_CATG'y
Cuts at: 1167 2370 3031 3531 4438 1167
Size: 1203 661 500 907 2707
Fragments arranged by size:
2707 1203 907 661 500
PciI A'CATG_T
Cuts at: 4434 4434
Size: 5978
PflMI CCAn_nnn'nTGG
Cuts at: 708 2736 708
Size: 2028 3950
PfoI T'CCnGG_A
Cuts at: 839 2786 839
Size: 1947 4031
PmeI GTTT'AAAC
Cuts at: 2977 2977
Size: 5978
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 406 438 5117 5149 406
Size: 32 4679 32 1235
Fragments arranged by size:
4679 1235 32 32
PpuMI rG'GwC_Cy
Cuts at: 1647 2007 2227 2301 1647
Size: 360 220 74 5324
Fragments arranged by size:
5324 360 220 74
PshAI GACnn'nnGTC
Cuts at: 781 781
Size: 5978
PspOMI G'GGCC_C
Cuts at: 2194 2194
Size: 5978
PstI C_TGCA'G
Cuts at: 905 1124 1838 2136 2327 2855 3025 3710 905
Size: 219 714 298 191 528 170 685 3173
Fragments arranged by size:
3173 714 685 528 298 219 191 170
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5978
PvuII CAG'CTG
Cuts at: 1107 1427 1812 2319 2454 2685 3403 3763 1107
Size: 320 385 507 135 231 718 360 3322
Fragments arranged by size:
3322 718 507 385 360 320 231 135
RsrII CG'GwC_CG
Cuts at: 4173 4173
Size: 5978
SacI G_AGCT'C
Cuts at: 2992 2992
Size: 5978
SalI G'TCGA_C
Cuts at: 2061 3015 2061
Size: 954 5024
SanDI GG'GwC_CC
Cuts at: 1647 2007 1647
Size: 360 5618
SapI GCTCTTCn'nnn_
Cuts at: 4001 4211 4001
Size: 210 5768
SbfI CC_TGCA'GG
Cuts at: 2855 3025 2855
Size: 170 5808
ScaI AGT'ACT
Cuts at: 5495 5495
Size: 5978
SexAI A'CCwGG_T
Cuts at: 650 650
Size: 5978
SfcI C'TryA_G
Cuts at: 33 264 901 1120 1422 1504 1834 2132 2323
Size: 231 637 219 302 82 330 298 191
Cuts at: 2323 2851 3021 3706 4699 4890 33
Size: 528 170 685 993 191 1121
Fragments arranged by size:
1121 993 685 637 528 330 302 298
231 219 191 191 170 82
SfiI GGCCn_nnn'nGGCC
Cuts at: 1399 1399
Size: 5978
SfoI GGC'GCC
Cuts at: 2586 3410 3658 2586
Size: 824 248 4906
Fragments arranged by size:
4906 824 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5978
SmaI CCC'GGG
Cuts at: 2435 3000 2435
Size: 565 5413
SmlI C'TyrA_G
Cuts at: 1237 3133 4048 4540 4802 1237
Size: 1896 915 492 262 2413
Fragments arranged by size:
2413 1896 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5978
SphI G_CATG'C
Cuts at: 3031 3531 3031
Size: 500 5478
StuI AGG'CCT
Cuts at: 544 640 2212 544
Size: 96 1572 4310
Fragments arranged by size:
4310 1572 96
StyI C'CwwG_G
Cuts at: 122 254 659 1617 1772 2735 3112 3466 122
Size: 132 405 958 155 963 377 354 2634
Fragments arranged by size:
2634 963 958 405 377 354 155 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 700 2433 3561 3694 4153 4362 700
Size: 1733 1128 133 459 209 2316
Fragments arranged by size:
2316 1733 1128 459 209 133
TatI w'GTAC_w
Cuts at: 632 5493 632
Size: 4861 1117
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 663 695 1150 1182 5495 5527 663
Size: 32 455 32 4313 32 1114
Fragments arranged by size:
4313 1114 455 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 595 1222 3775 595
Size: 627 2553 2798
Fragments arranged by size:
2798 2553 627
XbaI T'CTAG_A
Cuts at: 3009 3009
Size: 5978
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2733 3473 2733
Size: 740 5238
XmaI C'CCGG_G
Cuts at: 2433 2998 2433
Size: 565 5413
XmnI GAAnn'nnTTC
Cuts at: 2716 2716
Size: 5978
ZraI GAC'GTC
Cuts at: 1254 1530 1887 2160 1254
Size: 276 357 273 5072
Fragments arranged by size:
5072 357 276 273
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AlfI AloI AscI AvrII BaeI BfrBI
BmtI BsiWI BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PsiI PspXI PsrI SacII
SgrAI SnaBI SrfI SspI SwaI XhoI