Restriction map for
pF1KB8506
(Circular) (Six-base) MAPSORT of: pF1KB8506.seq Check: 300 from: 1 to: 5711
pF1KB8506 5711 bp
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AccI GT'mk_AC
Cuts at: 959 2749 959
Size: 1790 3921
Acc65I G'GTAC_C
Cuts at: 2727 2727
Size: 5711
AclI AA'CG_TT
Cuts at: 2705 5551 2705
Size: 2846 2865
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 778 788 1144 1229 1322 3536 3968 4715
Size: 75 10 356 85 93 2214 432 747
Cuts at: 4715 4983 703
Size: 268 1431
Fragments arranged by size:
2214 1431 747 432 356 268 93 85
75 10
AflIII A'CryG_T
Cuts at: 228 238 3063 4167 5144 228
Size: 10 2825 1104 977 795
Fragments arranged by size:
2825 1104 977 795 10
AgeI A'CCGG_T
Cuts at: 4099 4099
Size: 5711
AhdI GACnn_n'nnGTC
Cuts at: 966 966
Size: 5711
AleI CACnn'nnGTG
Cuts at: 1330 1814 1330
Size: 484 5227
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 146 146
Size: 5711
AlwNI CAG_nnn'CTG
Cuts at: 298 519 1569 2586 3016 4583 298
Size: 221 1050 1017 430 1567 1426
Fragments arranged by size:
1567 1426 1050 1017 430 221
ApaI G_GGCC'C
Cuts at: 2641 2641
Size: 5711
ApaLI G'TGCA_C
Cuts at: 4481 4481
Size: 5711
ApoI r'AATT_y
Cuts at: 1255 1900 2715 5064 5320 1255
Size: 645 815 2349 256 1646
Fragments arranged by size:
2349 1646 815 645 256
AseI AT'TA_AT
Cuts at: 20 1595 20
Size: 1575 4136
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5711
AvaI C'yCGr_G
Cuts at: 1276 1533 2731 1276
Size: 257 1198 4256
Fragments arranged by size:
4256 1198 257
BamHI G'GATC_C
Cuts at: 601 744 2736 601
Size: 143 1992 3576
Fragments arranged by size:
3576 1992 143
BanI G'GyrC_C
Cuts at: 2727 3141 3389 3424 2727
Size: 414 248 35 5014
Fragments arranged by size:
5014 414 248 35
BanII G_rGCy'C
Cuts at: 1784 2185 2641 2725 3755 1784
Size: 401 456 84 1030 3740
Fragments arranged by size:
3740 1030 456 401 84
BbeI G_GCGC'C
Cuts at: 3145 3393 3145
Size: 248 5463
BbsI GAAGACnn'nnnn_
Cuts at: 457 457
Size: 5711
BbvCI CC'TCA_GC
Cuts at: 1456 1456
Size: 5711
BciVI GTATCCnnnnn_n'
Cuts at: 3609 4370 5711 3609
Size: 761 1341 3609
Fragments arranged by size:
3609 1341 761
BclI T'GATC_A
Cuts at: 3231 3231
Size: 5711
BfrBI ATG'CAT
Cuts at: 2621 2621
Size: 5711
BglII A'GATC_T
Cuts at: 585 3226 5263 585
Size: 2641 2037 1033
Fragments arranged by size:
2641 2037 1033
BlpI GC'TnA_GC
Cuts at: 1676 2823 1676
Size: 1147 4564
Bme1580I G_kGCm'C
Cuts at: 2088 2641 3336 3429 4485 2088
Size: 553 695 93 1056 3314
Fragments arranged by size:
3314 1056 695 553 93
BmrI ACTGGGnnnn_n'
Cuts at: 318 504 1188 2923 3004 3339 5483 318
Size: 186 684 1735 81 335 2144 546
Fragments arranged by size:
2144 1735 684 546 335 186 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1590 1747 4078 1590
Size: 157 2331 3223
Fragments arranged by size:
3223 2331 157
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 581 1136 1339 1381 1768 2345 295
Size: 286 555 203 42 387 577 3661
Fragments arranged by size:
3661 577 555 387 286 203 42
Bpu10I CC'TnA_GC
Cuts at: 1456 1456
Size: 5711
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2887 3766 4258 4556 2887
Size: 879 492 298 4042
Fragments arranged by size:
4042 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 295 974 5415 295
Size: 679 4441 591
Fragments arranged by size:
4441 679 591
BsaAI yAC'GTr
Cuts at: 3064 3064
Size: 5711
BsaBI GATnn'nnATC
Cuts at: 6 3225 5262 6
Size: 3219 2037 455
Fragments arranged by size:
3219 2037 455
BsaHI Gr'CG_yC
Cuts at: 3142 3390 4092 5588 3142
Size: 248 702 1496 3265
Fragments arranged by size:
3265 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2900 3421 4099 4373 4520 4931 2900
Size: 521 678 274 147 411 3680
Fragments arranged by size:
3680 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1454 2121 2142 2310 1454
Size: 667 21 168 4855
Fragments arranged by size:
4855 667 168 21
BseYI C'CCAG_C
Cuts at: 170 1762 2175 3136 3894 4471 4967 170
Size: 1592 413 961 758 577 496 914
Fragments arranged by size:
1592 961 914 758 577 496 413
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1132 1132
Size: 5711
BsiEI CG_ry'CG
Cuts at: 104 3253 3299 4507 104
Size: 3149 46 1208 1308
Fragments arranged by size:
3149 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1476 1784 2541 2725 3503 3693 4485 1476
Size: 308 757 184 778 190 792 2702
Fragments arranged by size:
2702 792 778 757 308 190 184
BsmI GAATG_Cn'
Cuts at: 920 1936 2623 920
Size: 1016 687 4008
Fragments arranged by size:
4008 1016 687
Bsp1286I G_dGCh'C
Cuts at: 1476 1784 2088 2185 2541 2641 2725 3336 3429
Size: 308 304 97 356 100 84 611 93
Cuts at: 3429 3503 3693 3755 4485 1476
Size: 74 190 62 730 2702
Fragments arranged by size:
2702 730 611 356 308 304 190 100
97 93 84 74 62
BspEI T'CCGG_A
Cuts at: 4931 4931
Size: 5711
BspHI T'CATG_A
Cuts at: 4887 4887
Size: 5711
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2761 2975 3277 3658 2761
Size: 214 302 381 4814
Fragments arranged by size:
4814 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4003 4057 5541 5706 50
Size: 3953 54 1484 165 55
Fragments arranged by size:
3953 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 590 3623 590
Size: 3033 2678
BsrFI r'CCGG_y
Cuts at: 139 1907 3709 3890 4099 139
Size: 1768 1802 181 209 1751
Fragments arranged by size:
1802 1768 1751 209 181
BssSI C'ACGA_G
Cuts at: 1475 1546 3982 4340 1475
Size: 71 2436 358 2846
Fragments arranged by size:
2846 2436 358 71
BstAPI GCAn_nnn'nTGC
Cuts at: 3088 3088
Size: 5711
BstBI TT'CG_AA
Cuts at: 4072 4072
Size: 5711
BstXI CCAn_nnnn'nTGG
Cuts at: 2197 2197
Size: 5711
BstYI r'GATC_y
Cuts at: 1 294 585 601 744 980 2524 2736 3203
Size: 293 291 16 143 236 1544 212 467
Cuts at: 3203 3226 3561 3807 4819 4905 4917 5263 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1544 1012 467 449 346 335 293 291
246 236 212 143 86 23 16 12
Bsu36I CC'TnA_GG
Cuts at: 1555 1555
Size: 5711
BtgI C'CryG_G
Cuts at: 1409 1954 1409
Size: 545 5166
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2336 3661 3842 4005 2336
Size: 1325 181 163 4042
Fragments arranged by size:
4042 1325 181 163
BtsI GCAGTG_nn'
Cuts at: 1120 1325 1337 1508 2954 1120
Size: 205 12 171 1446 3877
Fragments arranged by size:
3877 1446 205 171 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1182 1217 1182
Size: 35 5676
DraI TTT'AAA
Cuts at: 225 2710 225
Size: 2485 3226
DraIII CAC_nnn'GTG
Cuts at: 2062 2062
Size: 5711
DrdI GACnn_nn'nnGTC
Cuts at: 614 3417 4275 614
Size: 2803 858 2050
Fragments arranged by size:
2803 2050 858
EaeI y'GGCC_r
Cuts at: 851 1446 3296 3470 3861 3888 5233 851
Size: 595 1850 174 391 27 1345 1329
Fragments arranged by size:
1850 1345 1329 595 391 174 27
EagI C'GGCC_G
Cuts at: 3296 3296
Size: 5711
EarI CTCTTCn'nnn_
Cuts at: 149 863 869 1951 2328 3734 3944 149
Size: 714 6 1082 377 1406 210 1916
Fragments arranged by size:
1916 1406 1082 714 377 210 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2133 3919 4229 4375 5521 2133
Size: 1786 310 146 1146 2323
Fragments arranged by size:
2323 1786 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1782 2723 1782
Size: 941 4770
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 581 703 778 788 1136 1144 1229 1322
Size: 286 122 75 10 348 8 85 93
Cuts at: 1322 1339 1381 1768 2345 3536 3968 4715 4983
Size: 17 42 387 577 1191 432 747 268
Cuts at: 4983 295
Size: 1023
Fragments arranged by size:
1191 1023 747 577 432 387 348 286
268 122 93 85 75 42 17 10
8
EcoNI CCTnn'n_nnAGG
Cuts at: 1723 2563 1723
Size: 840 4871
EcoO109I rG'GnC_Cy
Cuts at: 558 1551 1696 1728 2583 2638 2850 558
Size: 993 145 32 855 55 212 3419
Fragments arranged by size:
3419 993 855 212 145 55 32
EcoRI G'AATT_C
Cuts at: 1900 2715 1900
Size: 815 4896
EcoRV GAT'ATC
Cuts at: 2345 2345
Size: 5711
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1641 3730 1641
Size: 2089 3622
FspI TGC'GCA
Cuts at: 3492 3492
Size: 5711
HaeII r_GCGC'y
Cuts at: 3145 3393 4415 5394 3145
Size: 248 1022 979 3462
Fragments arranged by size:
3462 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 127 159 279 311 472 504 952 984 1783
Size: 32 120 32 161 32 448 32 799
Cuts at: 1783 1815 3553 3583 3585 3615 127
Size: 32 1738 30 2 30 2223
Fragments arranged by size:
2223 1738 799 448 161 120 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2750 2750
Size: 5711
HindIII A'AGCT_T
Cuts at: 620 620
Size: 5711
KasI G'GCGC_C
Cuts at: 3141 3389 3141
Size: 248 5463
KpnI G_GTAC'C
Cuts at: 2731 2731
Size: 5711
MluI A'CGCG_T
Cuts at: 5144 5144
Size: 5711
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4382 4566 4382
Size: 184 5527
MscI TGG'CCA
Cuts at: 853 3472 853
Size: 2619 3092
MslI CAynn'nnrTG
Cuts at: 1330 1814 3700 3827 1330
Size: 484 1886 127 3214
Fragments arranged by size:
3214 1886 484 127
MspA1I CmG'CkG
Cuts at: 549 1359 1788 2179 2822 3136 3496 4509 4754
Size: 810 429 391 643 314 360 1013 245
Cuts at: 4754 549
Size: 1506
Fragments arranged by size:
1506 1013 810 643 429 391 360 314
245
NaeI GCC'GGC
Cuts at: 141 1909 3892 141
Size: 1768 1983 1960
Fragments arranged by size:
1983 1960 1768
NarI GG'CG_CC
Cuts at: 3142 3390 3142
Size: 248 5463
NcoI C'CATG_G
Cuts at: 1409 1409
Size: 5711
NgoMIV G'CCGG_C
Cuts at: 139 1907 3890 139
Size: 1768 1983 1960
Fragments arranged by size:
1983 1960 1768
NsiI A_TGCA'T
Cuts at: 2623 2623
Size: 5711
NspI r_CATG'y
Cuts at: 232 533 2764 3264 4171 232
Size: 301 2231 500 907 1772
Fragments arranged by size:
2231 1772 907 500 301
PasI CC'CwG_GG
Cuts at: 2190 2190
Size: 5711
PciI A'CATG_T
Cuts at: 228 4167 228
Size: 3939 1772
PflMI CCAn_nnn'nTGG
Cuts at: 431 2062 431
Size: 1631 4080
PfoI T'CCnGG_A
Cuts at: 597 626 597
Size: 29 5682
PmeI GTTT'AAAC
Cuts at: 2710 2710
Size: 5711
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4850 4882 4850
Size: 32 5679
PpuMI rG'GwC_Cy
Cuts at: 1696 1728 1696
Size: 32 5679
PshAI GACnn'nnGTC
Cuts at: 1046 1046
Size: 5711
PspOMI G'GGCC_C
Cuts at: 2637 2637
Size: 5711
PspXI vC'TCGA_Gb
Cuts at: 1276 1533 1276
Size: 257 5454
PstI C_TGCA'G
Cuts at: 1332 2758 3443 1332
Size: 1426 685 3600
Fragments arranged by size:
3600 1426 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5711
PvuII CAG'CTG
Cuts at: 1359 1788 3136 3496 1359
Size: 429 1348 360 3574
Fragments arranged by size:
3574 1348 429 360
RsrII CG'GwC_CG
Cuts at: 3906 3906
Size: 5711
SacI G_AGCT'C
Cuts at: 1784 2725 1784
Size: 941 4770
SalI G'TCGA_C
Cuts at: 2748 2748
Size: 5711
SapI GCTCTTCn'nnn_
Cuts at: 869 3734 3944 869
Size: 2865 210 2636
Fragments arranged by size:
2865 2636 210
SbfI CC_TGCA'GG
Cuts at: 2758 2758
Size: 5711
ScaI AGT'ACT
Cuts at: 1021 5228 1021
Size: 4207 1504
SexAI A'CCwGG_T
Cuts at: 2058 2058
Size: 5711
SfcI C'TryA_G
Cuts at: 33 656 956 992 1328 2281 2754 3439 4432
Size: 623 300 36 336 953 473 685 993
Cuts at: 4432 4623 33
Size: 191 1121
Fragments arranged by size:
1121 993 953 685 623 473 336 300
191 36
SfoI GGC'GCC
Cuts at: 3143 3391 3143
Size: 248 5463
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5711
SmaI CCC'GGG
Cuts at: 2733 2733
Size: 5711
SmlI C'TyrA_G
Cuts at: 1276 1533 2866 3781 4273 4535 1276
Size: 257 1333 915 492 262 2452
Fragments arranged by size:
2452 1333 915 492 262 257
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5711
SphI G_CATG'C
Cuts at: 2764 3264 2764
Size: 500 5211
SspI AAT'ATT
Cuts at: 1606 2555 1606
Size: 949 4762
StyI C'CwwG_G
Cuts at: 1409 2845 3199 1409
Size: 1436 354 3921
Fragments arranged by size:
3921 1436 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 419 1939 2347 3294 3427 3886 4095 419
Size: 1520 408 947 133 459 209 2035
Fragments arranged by size:
2035 1520 947 459 408 209 133
TatI w'GTAC_w
Cuts at: 1019 1992 2257 5226 1019
Size: 973 265 2969 1504
Fragments arranged by size:
2969 1504 973 265
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 192 224 5228 5260 192
Size: 32 5004 32 643
Fragments arranged by size:
5004 643 32 32
Tth111I GACn'n_nGTC
Cuts at: 3508 3508
Size: 5711
XbaI T'CTAG_A
Cuts at: 183 2742 183
Size: 2559 3152
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2572 3206 2572
Size: 634 5077
XhoI C'TCGA_G
Cuts at: 1276 1533 1276
Size: 257 5454
XmaI C'CCGG_G
Cuts at: 2731 2731
Size: 5711
XmnI GAAnn'nnTTC
Cuts at: 2465 2465
Size: 5711
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI AscI AvrII BaeI
BcgI BglI BmgBI BmtI BsaXI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI FseI FspAI HpaI MfeI
NdeI NheI NotI NruI PacI PmlI PsiI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI
ZraI