Restriction map for
pF1KB8505
(Circular) (Six-base) MAPSORT of: pF1KB8505.seq Check: 8436 from: 1 to: 5864
pF1KB8505 5864 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 2902 2902
Size: 5864
Acc65I G'GTAC_C
Cuts at: 2880 2880
Size: 5864
AclI AA'CG_TT
Cuts at: 1128 1508 5704 1128
Size: 380 4196 1288
Fragments arranged by size:
4196 1288 380
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 564 1847 3689 4121 4868 5136 171
Size: 393 1283 1842 432 747 268 899
Fragments arranged by size:
1842 1283 899 747 432 393 268
AflII C'TTAA_G
Cuts at: 1914 1914
Size: 5864
AflIII A'CryG_T
Cuts at: 2083 3216 4320 5297 2083
Size: 1133 1104 977 2650
Fragments arranged by size:
2650 1133 1104 977
AgeI A'CCGG_T
Cuts at: 4252 4252
Size: 5864
AhdI GACnn_n'nnGTC
Cuts at: 695 695
Size: 5864
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2506 2506
Size: 5864
AlwNI CAG_nnn'CTG
Cuts at: 1486 3169 4736 1486
Size: 1683 1567 2614
Fragments arranged by size:
2614 1683 1567
ApaI G_GGCC'C
Cuts at: 2821 2821
Size: 5864
ApaLI G'TGCA_C
Cuts at: 2168 4634 2168
Size: 2466 3398
ApoI r'AATT_y
Cuts at: 447 1443 2868 5217 5473 447
Size: 996 1425 2349 256 838
Fragments arranged by size:
2349 1425 996 838 256
AseI AT'TA_AT
Cuts at: 20 514 20
Size: 494 5370
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5864
AvaI C'yCGr_G
Cuts at: 2884 2884
Size: 5864
AvrII C'CTAG_G
Cuts at: 1501 1501
Size: 5864
BamHI G'GATC_C
Cuts at: 2889 2889
Size: 5864
BanI G'GyrC_C
Cuts at: 2880 3294 3542 3577 2880
Size: 414 248 35 5167
Fragments arranged by size:
5167 414 248 35
BanII G_rGCy'C
Cuts at: 529 2543 2821 2878 3908 529
Size: 2014 278 57 1030 2485
Fragments arranged by size:
2485 2014 1030 278 57
BbeI G_GCGC'C
Cuts at: 3298 3546 3298
Size: 248 5616
BbsI GAAGACnn'nnnn_
Cuts at: 691 1260 1754 2582 691
Size: 569 494 828 3973
Fragments arranged by size:
3973 828 569 494
BciVI GTATCCnnnnn_n'
Cuts at: 3762 4523 5864 3762
Size: 761 1341 3762
Fragments arranged by size:
3762 1341 761
BclI T'GATC_A
Cuts at: 2055 3384 2055
Size: 1329 4535
BfrBI ATG'CAT
Cuts at: 2689 2689
Size: 5864
BglI GCCn_nnn'nGGC
Cuts at: 1471 1471
Size: 5864
BglII A'GATC_T
Cuts at: 3379 5416 3379
Size: 2037 3827
BlpI GC'TnA_GC
Cuts at: 772 2976 772
Size: 2204 3660
Bme1580I G_kGCm'C
Cuts at: 279 2172 2821 3489 3582 4638 279
Size: 1893 649 668 93 1056 1505
Fragments arranged by size:
1893 1505 1056 668 649 93
BmrI ACTGGGnnnn_n'
Cuts at: 282 824 1671 2034 2815 3076 3157 3492 5636
Size: 542 847 363 781 261 81 335 2144
Cuts at: 5636 282
Size: 510
Fragments arranged by size:
2144 847 781 542 510 363 335 261
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1572 4231 1572
Size: 2659 3205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 2865 514
Size: 2351 3513
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 602 2194 2401 3040 3919 4411 4709 602
Size: 1592 207 639 879 492 298 1757
Fragments arranged by size:
1757 1592 879 639 492 298 207
BsaI GGTCTCn'nnnn_
Cuts at: 5568 5568
Size: 5864
BsaAI yAC'GTr
Cuts at: 3217 3217
Size: 5864
BsaBI GATnn'nnATC
Cuts at: 6 1625 3378 5415 6
Size: 1619 1753 2037 455
Fragments arranged by size:
2037 1753 1619 455
BsaHI Gr'CG_yC
Cuts at: 2527 3295 3543 4245 5741 2527
Size: 768 248 702 1496 2650
Fragments arranged by size:
2650 1496 768 702 248
BsaWI w'CCGG_w
Cuts at: 2656 3053 3574 4252 4526 4673 5084 2656
Size: 397 521 678 274 147 411 3436
Fragments arranged by size:
3436 678 521 411 397 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2725 2755 2725
Size: 30 5834
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 305 2191 305
Size: 1886 3978
BseYI C'CCAG_C
Cuts at: 3289 4047 4624 5120 3289
Size: 758 577 496 4033
Fragments arranged by size:
4033 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2378 2378
Size: 5864
BsiEI CG_ry'CG
Cuts at: 104 3406 3452 4660 104
Size: 3302 46 1208 1308
Fragments arranged by size:
3302 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 529 2172 2543 2878 3656 3846 4638 529
Size: 1643 371 335 778 190 792 1755
Fragments arranged by size:
1755 1643 792 778 371 335 190
Bsp1286I G_dGCh'C
Cuts at: 279 529 2172 2543 2821 2878 3489 3582 3656
Size: 250 1643 371 278 57 611 93 74
Cuts at: 3656 3846 3908 4638 279
Size: 190 62 730 1505
Fragments arranged by size:
1643 1505 730 611 371 278 250 190
93 74 62 57
BspEI T'CCGG_A
Cuts at: 5084 5084
Size: 5864
BspHI T'CATG_A
Cuts at: 1080 1122 2829 5040 1080
Size: 42 1707 2211 1904
Fragments arranged by size:
2211 1904 1707 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2914 3128 3430 3811 2914
Size: 214 302 381 4967
Fragments arranged by size:
4967 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4156 4210 5694 5859 50
Size: 4106 54 1484 165 55
Fragments arranged by size:
4106 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 661 1058 1987 2199 2387 3776 661
Size: 397 929 212 188 1389 2749
Fragments arranged by size:
2749 1389 929 397 212 188
BsrFI r'CCGG_y
Cuts at: 3862 4043 4252 3862
Size: 181 209 5474
Fragments arranged by size:
5474 209 181
BssSI C'ACGA_G
Cuts at: 4135 4493 4135
Size: 358 5506
BstAPI GCAn_nnn'nTGC
Cuts at: 3241 3241
Size: 5864
BstBI TT'CG_AA
Cuts at: 1953 4225 1953
Size: 2272 3592
BstYI r'GATC_y
Cuts at: 1 1395 1992 2180 2889 3356 3379 3714 3960
Size: 1394 597 188 709 467 23 335 246
Cuts at: 3960 4972 5058 5070 5416 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1394 1012 709 597 467 449 346 335
246 188 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 533 533
Size: 5864
BtgI C'CryG_G
Cuts at: 107 548 107
Size: 441 5423
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3814 3995 4158 3814
Size: 181 163 5520
Fragments arranged by size:
5520 181 163
BtsI GCAGTG_nn'
Cuts at: 1481 3107 1481
Size: 1626 4238
DraI TTT'AAA
Cuts at: 117 2863 117
Size: 2746 3118
DrdI GACnn_nn'nnGTC
Cuts at: 3570 4428 3570
Size: 858 5006
EaeI y'GGCC_r
Cuts at: 485 3449 3623 4014 4041 5386 485
Size: 2964 174 391 27 1345 963
Fragments arranged by size:
2964 1345 963 391 174 27
EagI C'GGCC_G
Cuts at: 3449 3449
Size: 5864
EarI CTCTTCn'nnn_
Cuts at: 190 305 1604 3887 4097 190
Size: 115 1299 2283 210 1957
Fragments arranged by size:
2283 1957 1299 210 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4072 4382 4528 5674 4072
Size: 310 146 1146 4262
Fragments arranged by size:
4262 1146 310 146
EcoICRI GAG'CTC
Cuts at: 527 2541 2876 527
Size: 2014 335 3515
Fragments arranged by size:
3515 2014 335
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 514 564 1847 2865 3689 4121 4868 5136
Size: 343 50 1283 1018 824 432 747 268
Cuts at: 5136 171
Size: 899
Fragments arranged by size:
1283 1018 899 824 747 432 343 268
50
EcoO109I rG'GnC_Cy
Cuts at: 2225 2817 3003 2225
Size: 592 186 5086
Fragments arranged by size:
5086 592 186
EcoRI G'AATT_C
Cuts at: 2868 2868
Size: 5864
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1018 1222 3883 1018
Size: 204 2661 2999
Fragments arranged by size:
2999 2661 204
FspI TGC'GCA
Cuts at: 3645 3645
Size: 5864
HaeII r_GCGC'y
Cuts at: 3298 3546 4568 5547 3298
Size: 248 1022 979 3615
Fragments arranged by size:
3615 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 681 713 793 825 1597 1629 2512 2544 3706
Size: 32 80 32 772 32 883 32 1162
Cuts at: 3706 3736 3738 3768 681
Size: 30 2 30 2777
Fragments arranged by size:
2777 1162 883 772 80 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2505 2903 2505
Size: 398 5466
HindIII A'AGCT_T
Cuts at: 1718 2129 2310 1718
Size: 411 181 5272
Fragments arranged by size:
5272 411 181
HpaI GTT'AAC
Cuts at: 2505 2505
Size: 5864
KasI G'GCGC_C
Cuts at: 3294 3542 3294
Size: 248 5616
KpnI G_GTAC'C
Cuts at: 2884 2884
Size: 5864
MfeI C'AATT_G
Cuts at: 173 2366 2462 173
Size: 2193 96 3575
Fragments arranged by size:
3575 2193 96
MluI A'CGCG_T
Cuts at: 5297 5297
Size: 5864
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 416 4535 4719 416
Size: 4119 184 1561
Fragments arranged by size:
4119 1561 184
MscI TGG'CCA
Cuts at: 487 3625 487
Size: 3138 2726
MslI CAynn'nnrTG
Cuts at: 642 2834 3853 3980 642
Size: 2192 1019 127 2526
Fragments arranged by size:
2526 2192 1019 127
MspA1I CmG'CkG
Cuts at: 1771 2975 3289 3649 4662 4907 1771
Size: 1204 314 360 1013 245 2728
Fragments arranged by size:
2728 1204 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4045 4045
Size: 5864
NarI GG'CG_CC
Cuts at: 3295 3543 3295
Size: 248 5616
NcoI C'CATG_G
Cuts at: 107 548 107
Size: 441 5423
NgoMIV G'CCGG_C
Cuts at: 4043 4043
Size: 5864
NsiI A_TGCA'T
Cuts at: 2691 2691
Size: 5864
NspI r_CATG'y
Cuts at: 1038 1182 2087 2192 2917 3417 4324 1038
Size: 144 905 105 725 500 907 2578
Fragments arranged by size:
2578 907 905 725 500 144 105
PciI A'CATG_T
Cuts at: 2083 4320 2083
Size: 2237 3627
PfoI T'CCnGG_A
Cuts at: 2315 2647 2315
Size: 332 5532
PmeI GTTT'AAAC
Cuts at: 2863 2863
Size: 5864
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5003 5035 5003
Size: 32 5832
PpuMI rG'GwC_Cy
Cuts at: 2225 2225
Size: 5864
PshAI GACnn'nnGTC
Cuts at: 1049 1049
Size: 5864
PsiI TTA'TAA
Cuts at: 2030 2030
Size: 5864
PspOMI G'GGCC_C
Cuts at: 2817 2817
Size: 5864
PstI C_TGCA'G
Cuts at: 1418 2911 3596 1418
Size: 1493 685 3686
Fragments arranged by size:
3686 1493 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5864
PvuII CAG'CTG
Cuts at: 1771 3289 3649 1771
Size: 1518 360 3986
Fragments arranged by size:
3986 1518 360
RsrII CG'GwC_CG
Cuts at: 4059 4059
Size: 5864
SacI G_AGCT'C
Cuts at: 529 2543 2878 529
Size: 2014 335 3515
Fragments arranged by size:
3515 2014 335
SalI G'TCGA_C
Cuts at: 2901 2901
Size: 5864
SapI GCTCTTCn'nnn_
Cuts at: 305 3887 4097 305
Size: 3582 210 2072
Fragments arranged by size:
3582 2072 210
SbfI CC_TGCA'GG
Cuts at: 2911 2911
Size: 5864
ScaI AGT'ACT
Cuts at: 2663 5381 2663
Size: 2718 3146
SfcI C'TryA_G
Cuts at: 33 1414 2907 3592 4585 4776 33
Size: 1381 1493 685 993 191 1121
Fragments arranged by size:
1493 1381 1121 993 685 191
SfoI GGC'GCC
Cuts at: 3296 3544 3296
Size: 248 5616
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5864
SmaI CCC'GGG
Cuts at: 2886 2886
Size: 5864
SmlI C'TyrA_G
Cuts at: 617 1914 2173 2416 3019 3934 4426 4688 617
Size: 1297 259 243 603 915 492 262 1793
Fragments arranged by size:
1793 1297 915 603 492 262 259 243
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5864
SphI G_CATG'C
Cuts at: 2917 3417 2917
Size: 500 5364
SspI AAT'ATT
Cuts at: 886 1149 1729 886
Size: 263 580 5021
Fragments arranged by size:
5021 580 263
StuI AGG'CCT
Cuts at: 2422 2422
Size: 5864
StyI C'CwwG_G
Cuts at: 107 548 1211 1501 2998 3352 107
Size: 441 663 290 1497 354 2619
Fragments arranged by size:
2619 1497 663 441 354 290
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3447 3580 4039 4248 3447
Size: 133 459 209 5063
Fragments arranged by size:
5063 459 209 133
TatI w'GTAC_w
Cuts at: 648 2661 5379 648
Size: 2013 2718 1133
Fragments arranged by size:
2718 2013 1133
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2311 2343 2725 2757 5381 5413 2311
Size: 32 382 32 2624 32 2762
Fragments arranged by size:
2762 2624 382 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3661 3661
Size: 5864
XbaI T'CTAG_A
Cuts at: 1686 2112 2895 1686
Size: 426 783 4655
Fragments arranged by size:
4655 783 426
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 861 1473 3359 861
Size: 612 1886 3366
Fragments arranged by size:
3366 1886 612
XmaI C'CCGG_G
Cuts at: 2884 2884
Size: 5864
XmnI GAAnn'nnTTC
Cuts at: 470 1202 1510 1760 2130 470
Size: 732 308 250 370 4204
Fragments arranged by size:
4204 732 370 308 250
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AleI AloI AscI BaeI BbvCI
BcgI BmgBI BmtI Bpu10I BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI NdeI NheI NotI NruI PacI
PasI PflMI PmlI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI