Restriction map for
pF1KB8503
(Circular) (Six-base) MAPSORT of: pF1KB8503.seq Check: 829 from: 1 to: 5924
pF1KB8503 5924 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 1121 2740 2962 1121
Size: 1619 222 4083
Fragments arranged by size:
4083 1619 222
Acc65I G'GTAC_C
Cuts at: 2940 2940
Size: 5924
AclI AA'CG_TT
Cuts at: 5764 5764
Size: 5924
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1190 1484 1773 1964 2018 2132 2152 3749 4181
Size: 294 289 191 54 114 20 1597 432
Cuts at: 4181 4928 5196 1190
Size: 747 268 1918
Fragments arranged by size:
1918 1597 747 432 294 289 268 191
114 54 20
AflIII A'CryG_T
Cuts at: 2479 3276 4380 5357 2479
Size: 797 1104 977 3046
Fragments arranged by size:
3046 1104 977 797
AgeI A'CCGG_T
Cuts at: 4312 4312
Size: 5924
AleI CACnn'nnGTG
Cuts at: 2798 2798
Size: 5924
AlwNI CAG_nnn'CTG
Cuts at: 1672 3229 4796 1672
Size: 1557 1567 2800
Fragments arranged by size:
2800 1567 1557
ApaI G_GGCC'C
Cuts at: 2606 2606
Size: 5924
ApaLI G'TGCA_C
Cuts at: 4694 4694
Size: 5924
ApoI r'AATT_y
Cuts at: 187 196 316 504 796 927 1078 1546 2069
Size: 9 120 188 292 131 151 468 523
Cuts at: 2069 2928 5277 5533 187
Size: 859 2349 256 578
Fragments arranged by size:
2349 859 578 523 468 292 256 188
151 131 120 9
AseI AT'TA_AT
Cuts at: 20 908 20
Size: 888 5036
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5924
AvaI C'yCGr_G
Cuts at: 2944 2944
Size: 5924
BamHI G'GATC_C
Cuts at: 2652 2949 2652
Size: 297 5627
BanI G'GyrC_C
Cuts at: 2940 3354 3602 3637 2940
Size: 414 248 35 5227
Fragments arranged by size:
5227 414 248 35
BanII G_rGCy'C
Cuts at: 2393 2606 2938 3968 2393
Size: 213 332 1030 4349
Fragments arranged by size:
4349 1030 332 213
BbeI G_GCGC'C
Cuts at: 3358 3606 3358
Size: 248 5676
BbsI GAAGACnn'nnnn_
Cuts at: 1490 2758 1490
Size: 1268 4656
BbvCI CC'TCA_GC
Cuts at: 791 791
Size: 5924
BciVI GTATCCnnnnn_n'
Cuts at: 827 3822 4583 5924 827
Size: 2995 761 1341 827
Fragments arranged by size:
2995 1341 827 761
BclI T'GATC_A
Cuts at: 2490 3444 2490
Size: 954 4970
BfrBI ATG'CAT
Cuts at: 1214 2722 2850 1214
Size: 1508 128 4288
Fragments arranged by size:
4288 1508 128
BglII A'GATC_T
Cuts at: 3439 5476 3439
Size: 2037 3887
BlpI GC'TnA_GC
Cuts at: 3036 3036
Size: 5924
Bme1580I G_kGCm'C
Cuts at: 2360 2606 3549 3642 4698 2360
Size: 246 943 93 1056 3586
Fragments arranged by size:
3586 1056 943 246 93
BmrI ACTGGGnnnn_n'
Cuts at: 3136 3217 3552 5696 3136
Size: 81 335 2144 3364
Fragments arranged by size:
3364 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4291 4291
Size: 5924
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 545 664 1934 2083 2550 545
Size: 119 1270 149 467 3919
Fragments arranged by size:
3919 1270 467 149 119
Bpu10I CC'TnA_GC
Cuts at: 791 791
Size: 5924
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1448 2268 2685 2885 3100 3979 4471 4769 1448
Size: 820 417 200 215 879 492 298 2603
Fragments arranged by size:
2603 879 820 492 417 298 215 200
BsaI GGTCTCn'nnnn_
Cuts at: 1868 5628 1868
Size: 3760 2164
BsaAI yAC'GTr
Cuts at: 3277 3277
Size: 5924
BsaBI GATnn'nnATC
Cuts at: 6 3438 5475 6
Size: 3432 2037 455
Fragments arranged by size:
3432 2037 455
BsaHI Gr'CG_yC
Cuts at: 3355 3603 4305 5801 3355
Size: 248 702 1496 3478
Fragments arranged by size:
3478 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2655 3113 3634 4312 4586 4733 5144 2655
Size: 458 521 678 274 147 411 3435
Fragments arranged by size:
3435 678 521 458 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2523 2553 2523
Size: 30 5894
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1594 1594
Size: 5924
BseYI C'CCAG_C
Cuts at: 2871 3349 4107 4684 5180 2871
Size: 478 758 577 496 3615
Fragments arranged by size:
3615 758 577 496 478
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 878 878
Size: 5924
BsiEI CG_ry'CG
Cuts at: 104 3466 3512 4720 104
Size: 3362 46 1208 1308
Fragments arranged by size:
3362 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1206 1508 2795 2938 3716 3906 4698 1206
Size: 302 1287 143 778 190 792 2432
Fragments arranged by size:
2432 1287 792 778 302 190 143
BsmI GAATG_Cn'
Cuts at: 1830 1830
Size: 5924
Bsp1286I G_dGCh'C
Cuts at: 1206 1508 2360 2393 2606 2795 2938 3549 3642
Size: 302 852 33 213 189 143 611 93
Cuts at: 3642 3716 3906 3968 4698 1206
Size: 74 190 62 730 2432
Fragments arranged by size:
2432 852 730 611 302 213 190 189
143 93 74 62 33
BspEI T'CCGG_A
Cuts at: 2655 5144 2655
Size: 2489 3435
BspHI T'CATG_A
Cuts at: 312 5100 312
Size: 4788 1136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2974 3188 3490 3871 2974
Size: 214 302 381 5027
Fragments arranged by size:
5027 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4216 4270 5754 5919 50
Size: 4166 54 1484 165 55
Fragments arranged by size:
4166 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 627 1386 1761 3836 627
Size: 759 375 2075 2715
Fragments arranged by size:
2715 2075 759 375
BsrFI r'CCGG_y
Cuts at: 3922 4103 4312 3922
Size: 181 209 5534
Fragments arranged by size:
5534 209 181
BsrGI T'GTAC_A
Cuts at: 2482 2482
Size: 5924
BssSI C'ACGA_G
Cuts at: 4195 4553 4195
Size: 358 5566
BstAPI GCAn_nnn'nTGC
Cuts at: 808 3301 808
Size: 2493 3431
BstBI TT'CG_AA
Cuts at: 126 2075 4285 126
Size: 1949 2210 1765
Fragments arranged by size:
2210 1949 1765
BstYI r'GATC_y
Cuts at: 1 2652 2949 3416 3439 3774 4020 5032 5118
Size: 2651 297 467 23 335 246 1012 86
Cuts at: 5118 5130 5476 1
Size: 12 346 449
Fragments arranged by size:
2651 1012 467 449 346 335 297 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 689 689
Size: 5924
BtgI C'CryG_G
Cuts at: 107 2606 107
Size: 2499 3425
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3874 4055 4218 3874
Size: 181 163 5580
Fragments arranged by size:
5580 181 163
BtsI GCAGTG_nn'
Cuts at: 383 674 1063 2544 3167 383
Size: 291 389 1481 623 3140
Fragments arranged by size:
3140 1481 623 389 291
DraI TTT'AAA
Cuts at: 509 866 2923 509
Size: 357 2057 3510
Fragments arranged by size:
3510 2057 357
DrdI GACnn_nn'nnGTC
Cuts at: 3630 4488 3630
Size: 858 5066
EaeI y'GGCC_r
Cuts at: 2010 3509 3683 4074 4101 5446 2010
Size: 1499 174 391 27 1345 2488
Fragments arranged by size:
2488 1499 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3509 3509
Size: 5924
EarI CTCTTCn'nnn_
Cuts at: 141 272 1487 2108 2329 2585 2783 3947 4157
Size: 131 1215 621 221 256 198 1164 210
Cuts at: 4157 141
Size: 1908
Fragments arranged by size:
1908 1215 1164 621 256 221 210 198
131
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4132 4442 4588 5734 4132
Size: 310 146 1146 4322
Fragments arranged by size:
4322 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2936 2936
Size: 5924
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 545 664 1190 1484 1773 1934 1964 2018 2083
Size: 119 526 294 289 161 30 54 65
Cuts at: 2083 2132 2152 2550 3749 4181 4928 5196 545
Size: 49 20 398 1199 432 747 268 1273
Fragments arranged by size:
1273 1199 747 526 432 398 294 289
268 161 119 65 54 49 30 20
EcoNI CCTnn'n_nnAGG
Cuts at: 407 407
Size: 5924
EcoO109I rG'GnC_Cy
Cuts at: 3063 3063
Size: 5924
EcoRI G'AATT_C
Cuts at: 316 927 2928 316
Size: 611 2001 3312
Fragments arranged by size:
3312 2001 611
EcoRV GAT'ATC
Cuts at: 1386 2229 1386
Size: 843 5081
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1195 3943 1195
Size: 2748 3176
FspI TGC'GCA
Cuts at: 3705 3705
Size: 5924
HaeII r_GCGC'y
Cuts at: 3358 3606 4628 5607 3358
Size: 248 1022 979 3675
Fragments arranged by size:
3675 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1408 1440 1612 1644 2605 2637 3766 3796 3798
Size: 32 172 32 961 32 1129 30 2
Cuts at: 3798 3828 1408
Size: 30 3504
Fragments arranged by size:
3504 1129 961 172 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2672 2882 2963 2672
Size: 210 81 5633
Fragments arranged by size:
5633 210 81
HindIII A'AGCT_T
Cuts at: 345 957 2284 345
Size: 612 1327 3985
Fragments arranged by size:
3985 1327 612
HpaI GTT'AAC
Cuts at: 2672 2882 2672
Size: 210 5714
KasI G'GCGC_C
Cuts at: 3354 3602 3354
Size: 248 5676
KpnI G_GTAC'C
Cuts at: 2944 2944
Size: 5924
MfeI C'AATT_G
Cuts at: 1642 1642
Size: 5924
MluI A'CGCG_T
Cuts at: 5357 5357
Size: 5924
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 519 653 1084 4595 4779 519
Size: 134 431 3511 184 1664
Fragments arranged by size:
3511 1664 431 184 134
MscI TGG'CCA
Cuts at: 2012 3685 2012
Size: 1673 4251
MslI CAynn'nnrTG
Cuts at: 127 1520 2798 3913 4040 127
Size: 1393 1278 1115 127 2011
Fragments arranged by size:
2011 1393 1278 1115 127
MspA1I CmG'CkG
Cuts at: 1129 1798 3035 3349 3709 4722 4967 1129
Size: 669 1237 314 360 1013 245 2086
Fragments arranged by size:
2086 1237 1013 669 360 314 245
NaeI GCC'GGC
Cuts at: 4105 4105
Size: 5924
NarI GG'CG_CC
Cuts at: 3355 3603 3355
Size: 248 5676
NcoI C'CATG_G
Cuts at: 107 107
Size: 5924
NgoMIV G'CCGG_C
Cuts at: 4103 4103
Size: 5924
NsiI A_TGCA'T
Cuts at: 1216 2724 2852 1216
Size: 1508 128 4288
Fragments arranged by size:
4288 1508 128
NspI r_CATG'y
Cuts at: 2084 2483 2854 2977 3477 4384 2084
Size: 399 371 123 500 907 3624
Fragments arranged by size:
3624 907 500 399 371 123
PciI A'CATG_T
Cuts at: 2479 4380 2479
Size: 1901 4023
PfoI T'CCnGG_A
Cuts at: 561 1950 2498 561
Size: 1389 548 3987
Fragments arranged by size:
3987 1389 548
PmeI GTTT'AAAC
Cuts at: 2923 2923
Size: 5924
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5063 5095 5063
Size: 32 5892
PshAI GACnn'nnGTC
Cuts at: 2736 2736
Size: 5924
PspOMI G'GGCC_C
Cuts at: 2602 2602
Size: 5924
PstI C_TGCA'G
Cuts at: 2427 2971 3656 2427
Size: 544 685 4695
Fragments arranged by size:
4695 685 544
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5924
PvuII CAG'CTG
Cuts at: 1129 1798 3349 3709 1129
Size: 669 1551 360 3344
Fragments arranged by size:
3344 1551 669 360
RsrII CG'GwC_CG
Cuts at: 4119 4119
Size: 5924
SacI G_AGCT'C
Cuts at: 2938 2938
Size: 5924
SalI G'TCGA_C
Cuts at: 2961 2961
Size: 5924
SapI GCTCTTCn'nnn_
Cuts at: 2783 3947 4157 2783
Size: 1164 210 4550
Fragments arranged by size:
4550 1164 210
SbfI CC_TGCA'GG
Cuts at: 2971 2971
Size: 5924
ScaI AGT'ACT
Cuts at: 5441 5441
Size: 5924
SexAI A'CCwGG_T
Cuts at: 1527 1527
Size: 5924
SfcI C'TryA_G
Cuts at: 33 2221 2423 2741 2967 3652 4645 4836 33
Size: 2188 202 318 226 685 993 191 1121
Fragments arranged by size:
2188 1121 993 685 318 226 202 191
SfoI GGC'GCC
Cuts at: 3356 3604 3356
Size: 248 5676
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5924
SmaI CCC'GGG
Cuts at: 2946 2946
Size: 5924
SmlI C'TyrA_G
Cuts at: 1463 2247 2700 2900 3079 3994 4486 4748 1463
Size: 784 453 200 179 915 492 262 2639
Fragments arranged by size:
2639 915 784 492 453 262 200 179
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5924
SphI G_CATG'C
Cuts at: 2854 2977 3477 2854
Size: 123 500 5301
Fragments arranged by size:
5301 500 123
SspI AAT'ATT
Cuts at: 1589 1740 1941 1589
Size: 151 201 5572
Fragments arranged by size:
5572 201 151
StuI AGG'CCT
Cuts at: 1055 2179 1055
Size: 1124 4800
StyI C'CwwG_G
Cuts at: 107 272 2297 2396 2855 3058 3412 107
Size: 165 2025 99 459 203 354 2619
Fragments arranged by size:
2619 2025 459 354 203 165 99
SwaI ATTT'AAAT
Cuts at: 509 509
Size: 5924
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2434 3507 3640 4099 4308 2434
Size: 1073 133 459 209 4050
Fragments arranged by size:
4050 1073 459 209 133
TatI w'GTAC_w
Cuts at: 131 2482 5439 131
Size: 2351 2957 616
Fragments arranged by size:
2957 2351 616
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2523 2555 5441 5473 2523
Size: 32 2886 32 2974
Fragments arranged by size:
2974 2886 32 32
Tth111I GACn'n_nGTC
Cuts at: 3721 3721
Size: 5924
XbaI T'CTAG_A
Cuts at: 2955 2955
Size: 5924
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 486 2239 3419 486
Size: 1753 1180 2991
Fragments arranged by size:
2991 1753 1180
XmaI C'CCGG_G
Cuts at: 2944 2944
Size: 5924
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PfoI PmeI PpiI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BcgI BglI BmgBI BmtI BsiWI BsmBI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII FseI
FspAI NdeI NheI NotI NruI PacI PasI PflMI
PmlI PpuMI PsiI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI XhoI XmnI ZraI