Restriction map for
pF1KB8492
(Circular) (Six-base) MAPSORT of: pF1KB8492.seq Check: 8045 from: 1 to: 5687
pF1KB8492 5687 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1187 2358 1187
Size: 1171 4516
AatII G_ACGT'C
Cuts at: 2219 2228 2219
Size: 9 5678
AccI GT'mk_AC
Cuts at: 125 434 2725 125
Size: 309 2291 3087
Fragments arranged by size:
3087 2291 309
Acc65I G'GTAC_C
Cuts at: 736 2703 736
Size: 1967 3720
AclI AA'CG_TT
Cuts at: 5527 5527
Size: 5687
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 802 1135 1402 1459 1699 1801 3512 3944 4691
Size: 333 267 57 240 102 1711 432 747
Cuts at: 4691 4959 802
Size: 268 1530
Fragments arranged by size:
1711 1530 747 432 333 268 267 240
102 57
AflIII A'CryG_T
Cuts at: 170 2414 3039 4143 5120 170
Size: 2244 625 1104 977 737
Fragments arranged by size:
2244 1104 977 737 625
AgeI A'CCGG_T
Cuts at: 4075 4075
Size: 5687
AhdI GACnn_n'nnGTC
Cuts at: 501 2508 501
Size: 2007 3680
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 563 1126 2304 563
Size: 563 1178 3946
Fragments arranged by size:
3946 1178 563
AlwNI CAG_nnn'CTG
Cuts at: 1542 2565 2992 4559 1542
Size: 1023 427 1567 2670
Fragments arranged by size:
2670 1567 1023 427
ApaI G_GGCC'C
Cuts at: 1235 1403 2069 1235
Size: 168 666 4853
Fragments arranged by size:
4853 666 168
ApaLI G'TGCA_C
Cuts at: 4457 4457
Size: 5687
ApoI r'AATT_y
Cuts at: 1023 2691 5040 5296 1023
Size: 1668 2349 256 1414
Fragments arranged by size:
2349 1668 1414 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5687
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5687
AvaI C'yCGr_G
Cuts at: 254 424 619 1359 2707 254
Size: 170 195 740 1348 3234
Fragments arranged by size:
3234 1348 740 195 170
BamHI G'GATC_C
Cuts at: 2712 2712
Size: 5687
BanI G'GyrC_C
Cuts at: 391 736 1311 1555 1750 2074 2440 2703 3117
Size: 345 575 244 195 324 366 263 414
Cuts at: 3117 3365 3400 391
Size: 248 35 2678
Fragments arranged by size:
2678 575 414 366 345 324 263 248
244 195 35
BanII G_rGCy'C
Cuts at: 350 620 629 1220 1235 1403 1487 1991 2069
Size: 270 9 591 15 168 84 504 78
Cuts at: 2069 2336 2701 3731 350
Size: 267 365 1030 2306
Fragments arranged by size:
2306 1030 591 504 365 270 267 168
84 78 15 9
BbeI G_GCGC'C
Cuts at: 1754 3121 3369 1754
Size: 1367 248 4072
Fragments arranged by size:
4072 1367 248
BbsI GAAGACnn'nnnn_
Cuts at: 1347 1555 1347
Size: 208 5479
BbvCI CC'TCA_GC
Cuts at: 2039 2039
Size: 5687
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1231 1265 1231
Size: 34 5653
BciVI GTATCCnnnnn_n'
Cuts at: 3585 4346 5687 3585
Size: 761 1341 3585
Fragments arranged by size:
3585 1341 761
BclI T'GATC_A
Cuts at: 1166 3207 1166
Size: 2041 3646
BglI GCCn_nnn'nGGC
Cuts at: 707 853 1526 1636 707
Size: 146 673 110 4758
Fragments arranged by size:
4758 673 146 110
BglII A'GATC_T
Cuts at: 1772 2051 3202 5239 1772
Size: 279 1151 2037 2220
Fragments arranged by size:
2220 2037 1151 279
BlpI GC'TnA_GC
Cuts at: 2799 2799
Size: 5687
Bme1580I G_kGCm'C
Cuts at: 1235 1403 1558 2069 3312 3405 4461 1235
Size: 168 155 511 1243 93 1056 2461
Fragments arranged by size:
2461 1243 1056 511 168 155 93
BmgBI CAC'GTC
Cuts at: 2034 2034
Size: 5687
BmrI ACTGGGnnnn_n'
Cuts at: 2532 2899 2980 3315 5459 2532
Size: 367 81 335 2144 2760
Fragments arranged by size:
2760 2144 367 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4054 4054
Size: 5687
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 634 1789 1810 622
Size: 12 1155 21 4499
Fragments arranged by size:
4499 1155 21 12
Bpu10I CC'TnA_GC
Cuts at: 2039 2039
Size: 5687
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1975 2863 3742 4234 4532 1975
Size: 888 879 492 298 3130
Fragments arranged by size:
3130 888 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1374 2567 2611 5391 1374
Size: 1193 44 2780 1670
Fragments arranged by size:
2780 1670 1193 44
BsaAI yAC'GTr
Cuts at: 189 1479 2415 3040 189
Size: 1290 936 625 2836
Fragments arranged by size:
2836 1290 936 625
BsaBI GATnn'nnATC
Cuts at: 6 1171 3201 5238 6
Size: 1165 2030 2037 455
Fragments arranged by size:
2037 2030 1165 455
BsaHI Gr'CG_yC
Cuts at: 1151 1751 2216 2225 2243 3118 3366 4068 5564
Size: 600 465 9 18 875 248 702 1496
Cuts at: 5564 1151
Size: 1274
Fragments arranged by size:
1496 1274 875 702 600 465 248 18
9
BsaWI w'CCGG_w
Cuts at: 2876 3397 4075 4349 4496 4907 2876
Size: 521 678 274 147 411 3656
Fragments arranged by size:
3656 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 820 850 1168 1198 2341 2371 820
Size: 30 318 30 1143 30 4136
Fragments arranged by size:
4136 1143 318 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 432 637 1051 1738 1807 2203 2437 432
Size: 205 414 687 69 396 234 3682
Fragments arranged by size:
3682 687 414 396 234 205 69
BseYI C'CCAG_C
Cuts at: 909 2315 2599 3112 3870 4447 4943 909
Size: 1406 284 513 758 577 496 1653
Fragments arranged by size:
1653 1406 758 577 513 496 284
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 1729 1747 2134 2218 538
Size: 1191 18 387 84 4007
Fragments arranged by size:
4007 1191 387 84 18
BsiEI CG_ry'CG
Cuts at: 104 1835 3229 3275 4483 104
Size: 1731 1394 46 1208 1308
Fragments arranged by size:
1731 1394 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 350 629 1220 1577 1877 1991 2701 3479 3669
Size: 279 591 357 300 114 710 778 190
Cuts at: 3669 4461 350
Size: 792 1576
Fragments arranged by size:
1576 792 778 710 591 357 300 279
190 114
BsmBI CGTCTCn'nnnn_
Cuts at: 1142 1842 1843 1142
Size: 700 1 4986
Fragments arranged by size:
4986 700 1
Bsp1286I G_dGCh'C
Cuts at: 350 620 629 1220 1235 1403 1487 1558 1577
Size: 270 9 591 15 168 84 71 19
Cuts at: 1577 1877 1991 2069 2336 2701 3312 3405 3479
Size: 300 114 78 267 365 611 93 74
Cuts at: 3479 3669 3731 4461 350
Size: 190 62 730 1576
Fragments arranged by size:
1576 730 611 591 365 300 270 267
190 168 114 93 84 78 74 71
62 19 15 9
BspEI T'CCGG_A
Cuts at: 4907 4907
Size: 5687
BspHI T'CATG_A
Cuts at: 716 1019 4863 716
Size: 303 3844 1540
Fragments arranged by size:
3844 1540 303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1187 1664 2358 2737 2951 3253 3634 1187
Size: 477 694 379 214 302 381 3240
Fragments arranged by size:
3240 694 477 381 379 302 214
BsrBI CCG'CTC
Cuts at: 50 698 852 3979 4033 5517 5682 50
Size: 648 154 3127 54 1484 165 55
Fragments arranged by size:
3127 1484 648 165 154 55 54
BsrDI GCAATG_nn'
Cuts at: 545 3599 545
Size: 3054 2633
BsrFI r'CCGG_y
Cuts at: 394 1753 1907 2077 2149 2437 3685 3866 4075
Size: 1359 154 170 72 288 1248 181 209
Cuts at: 4075 394
Size: 2006
Fragments arranged by size:
2006 1359 1248 288 209 181 170 154
72
BsrGI T'GTAC_A
Cuts at: 1913 1913
Size: 5687
BssHII G'CGCG_C
Cuts at: 2002 2002
Size: 5687
BssSI C'ACGA_G
Cuts at: 1522 3958 4316 1522
Size: 2436 358 2893
Fragments arranged by size:
2893 2436 358
BstAPI GCAn_nnn'nTGC
Cuts at: 974 1587 2565 3064 974
Size: 613 978 499 3597
Fragments arranged by size:
3597 978 613 499
BstBI TT'CG_AA
Cuts at: 4048 4048
Size: 5687
BstXI CCAn_nnnn'nTGG
Cuts at: 1668 2454 2607 1668
Size: 786 153 4748
Fragments arranged by size:
4748 786 153
BstYI r'GATC_y
Cuts at: 1 131 1772 2051 2351 2561 2712 3179 3202
Size: 130 1641 279 300 210 151 467 23
Cuts at: 3202 3537 3783 4795 4881 4893 5239 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1641 1012 467 449 346 335 300 279
246 210 151 130 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2506 2506
Size: 5687
BtgI C'CryG_G
Cuts at: 107 195 704 740 762 1253 1631 1703 2253
Size: 88 509 36 22 491 378 72 550
Cuts at: 2253 107
Size: 3541
Fragments arranged by size:
3541 550 509 491 378 88 72 36
22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 165 228 1728 3637 3818 3981 165
Size: 63 1500 1909 181 163 1871
Fragments arranged by size:
1909 1871 1500 181 163 63
BtsI GCAGTG_nn'
Cuts at: 1717 2930 1717
Size: 1213 4474
ClaI AT'CG_AT
Cuts at: 722 1958 722
Size: 1236 4451
DraI TTT'AAA
Cuts at: 2686 2686
Size: 5687
DraIII CAC_nnn'GTG
Cuts at: 2185 2185
Size: 5687
DrdI GACnn_nn'nnGTC
Cuts at: 1834 3393 4251 1834
Size: 1559 858 3270
Fragments arranged by size:
3270 1559 858
EaeI y'GGCC_r
Cuts at: 806 1628 1688 1946 2012 2147 2435 2523 3272
Size: 822 60 258 66 135 288 88 749
Cuts at: 3272 3446 3837 3864 5209 806
Size: 174 391 27 1345 1284
Fragments arranged by size:
1345 1284 822 749 391 288 258 174
135 88 66 60 27
EagI C'GGCC_G
Cuts at: 3272 3272
Size: 5687
EarI CTCTTCn'nnn_
Cuts at: 343 1856 3710 3920 343
Size: 1513 1854 210 2110
Fragments arranged by size:
2110 1854 1513 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1965 2629 3895 4205 4351 5497 1965
Size: 664 1266 310 146 1146 2155
Fragments arranged by size:
2155 1266 1146 664 310 146
EcoICRI GAG'CTC
Cuts at: 348 627 1218 1989 2699 348
Size: 279 591 771 710 3336
Fragments arranged by size:
3336 771 710 591 279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 634 802 1135 1402 1459 1699 1789 1801
Size: 12 168 333 267 57 240 90 12
Cuts at: 1801 1810 3512 3944 4691 4959 622
Size: 9 1702 432 747 268 1350
Fragments arranged by size:
1702 1350 747 432 333 268 267 240
168 90 57 12 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1114 1225 1792 1948 1114
Size: 111 567 156 4853
Fragments arranged by size:
4853 567 156 111
EcoO109I rG'GnC_Cy
Cuts at: 841 1231 1232 1282 1399 1428 1785 2634 2826
Size: 390 1 50 117 29 357 849 192
Cuts at: 2826 841
Size: 3702
Fragments arranged by size:
3702 849 390 357 192 117 50 29
1
EcoRI G'AATT_C
Cuts at: 2691 2691
Size: 5687
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1303 3706 1303
Size: 2403 3284
FspI TGC'GCA
Cuts at: 3468 3468
Size: 5687
HaeII r_GCGC'y
Cuts at: 1754 2289 3121 3369 4391 5370 1754
Size: 535 832 248 1022 979 2071
Fragments arranged by size:
2071 1022 979 832 535 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 427 459 487 519 1724 1756 2343 2375 2494
Size: 32 28 32 1205 32 587 32 119
Cuts at: 2494 2526 3529 3559 3561 3591 427
Size: 32 1003 30 2 30 2523
Fragments arranged by size:
2523 1205 1003 587 119 32 32 32
32 32 30 30 28 2
HincII GTy'rAC
Cuts at: 323 435 2478 2726 323
Size: 112 2043 248 3284
Fragments arranged by size:
3284 2043 248 112
KasI G'GCGC_C
Cuts at: 1750 3117 3365 1750
Size: 1367 248 4072
Fragments arranged by size:
4072 1367 248
KpnI G_GTAC'C
Cuts at: 740 2707 740
Size: 1967 3720
MluI A'CGCG_T
Cuts at: 5120 5120
Size: 5687
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1346 1721 2537 4358 4542 1346
Size: 375 816 1821 184 2491
Fragments arranged by size:
2491 1821 816 375 184
MscI TGG'CCA
Cuts at: 808 1690 3448 808
Size: 882 1758 3047
Fragments arranged by size:
3047 1758 882
MslI CAynn'nnrTG
Cuts at: 1591 2446 2452 3676 3803 1591
Size: 855 6 1224 127 3475
Fragments arranged by size:
3475 1224 855 127 6
MspA1I CmG'CkG
Cuts at: 612 981 1133 1211 1998 2255 2500 2798 3112
Size: 369 152 78 787 257 245 298 314
Cuts at: 3112 3472 4485 4730 612
Size: 360 1013 245 1569
Fragments arranged by size:
1569 1013 787 369 360 314 298 257
245 245 152 78
NaeI GCC'GGC
Cuts at: 396 1755 2151 2439 3868 396
Size: 1359 396 288 1429 2215
Fragments arranged by size:
2215 1429 1359 396 288
NarI GG'CG_CC
Cuts at: 1751 3118 3366 1751
Size: 1367 248 4072
Fragments arranged by size:
4072 1367 248
NcoI C'CATG_G
Cuts at: 107 195 704 1253 1703 107
Size: 88 509 549 450 4091
Fragments arranged by size:
4091 549 509 450 88
NgoMIV G'CCGG_C
Cuts at: 394 1753 2149 2437 3866 394
Size: 1359 396 288 1429 2215
Fragments arranged by size:
2215 1429 1359 396 288
NspI r_CATG'y
Cuts at: 174 433 1590 2199 2740 3240 4147 174
Size: 259 1157 609 541 500 907 1714
Fragments arranged by size:
1714 1157 907 609 541 500 259
PasI CC'CwG_GG
Cuts at: 1414 1414
Size: 5687
PciI A'CATG_T
Cuts at: 170 4143 170
Size: 3973 1714
PflMI CCAn_nnn'nTGG
Cuts at: 1542 2610 1542
Size: 1068 4619
PfoI T'CCnGG_A
Cuts at: 986 2669 986
Size: 1683 4004
PmeI GTTT'AAAC
Cuts at: 2686 2686
Size: 5687
PmlI CAC'GTG
Cuts at: 189 2415 189
Size: 2226 3461
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4826 4858 4826
Size: 32 5655
PpuMI rG'GwC_Cy
Cuts at: 1428 1785 1428
Size: 357 5330
PshAI GACnn'nnGTC
Cuts at: 1328 1328
Size: 5687
PspOMI G'GGCC_C
Cuts at: 1231 1399 2065 1231
Size: 168 666 4853
Fragments arranged by size:
4853 666 168
PstI C_TGCA'G
Cuts at: 611 2734 3419 611
Size: 2123 685 2879
Fragments arranged by size:
2879 2123 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5687
PvuII CAG'CTG
Cuts at: 612 981 3112 3472 612
Size: 369 2131 360 2827
Fragments arranged by size:
2827 2131 369 360
RsrII CG'GwC_CG
Cuts at: 3882 3882
Size: 5687
SacI G_AGCT'C
Cuts at: 350 629 1220 1991 2701 350
Size: 279 591 771 710 3336
Fragments arranged by size:
3336 771 710 591 279
SacII CC_GC'GG
Cuts at: 2256 2256
Size: 5687
SalI G'TCGA_C
Cuts at: 433 2724 433
Size: 2291 3396
SapI GCTCTTCn'nnn_
Cuts at: 3710 3920 3710
Size: 210 5477
SbfI CC_TGCA'GG
Cuts at: 2734 2734
Size: 5687
ScaI AGT'ACT
Cuts at: 5204 5204
Size: 5687
SfcI C'TryA_G
Cuts at: 33 607 2730 3415 4408 4599 33
Size: 574 2123 685 993 191 1121
Fragments arranged by size:
2123 1121 993 685 574 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1636 1636
Size: 5687
SfoI GGC'GCC
Cuts at: 1752 3119 3367 1752
Size: 1367 248 4072
Fragments arranged by size:
4072 1367 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5687
SgrAI Cr'CCGG_yG
Cuts at: 1753 1753
Size: 5687
SmaI CCC'GGG
Cuts at: 256 426 2709 256
Size: 170 2283 3234
Fragments arranged by size:
3234 2283 170
SmlI C'TyrA_G
Cuts at: 1990 2842 3757 4249 4511 1990
Size: 852 915 492 262 3166
Fragments arranged by size:
3166 915 852 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5687
SphI G_CATG'C
Cuts at: 2740 3240 2740
Size: 500 5187
SrfI GCCC'GGGC
Cuts at: 256 256
Size: 5687
StuI AGG'CCT
Cuts at: 2464 2464
Size: 5687
StyI C'CwwG_G
Cuts at: 107 195 704 821 1253 1703 2105 2168 2821
Size: 88 509 117 432 450 402 63 653
Cuts at: 2821 3175 107
Size: 354 2619
Fragments arranged by size:
2619 653 509 450 432 402 354 117
88 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3270 3403 3862 4071 3270
Size: 133 459 209 4886
Fragments arranged by size:
4886 459 209 133
TatI w'GTAC_w
Cuts at: 1913 5202 1913
Size: 3289 2398
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1168 1200 2341 2373 5204 5236 1168
Size: 32 1141 32 2831 32 1619
Fragments arranged by size:
2831 1619 1141 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1831 3484 1831
Size: 1653 4034
XbaI T'CTAG_A
Cuts at: 2718 2718
Size: 5687
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 193 2343 2607 3182 193
Size: 2150 264 575 2698
Fragments arranged by size:
2698 2150 575 264
XmaI C'CCGG_G
Cuts at: 254 424 2707 254
Size: 170 2283 3234
Fragments arranged by size:
3234 2283 170
ZraI GAC'GTC
Cuts at: 2217 2226 2217
Size: 9 5678
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AleI AloI AscI AvrII BaeI BfrBI
BmtI BsiWI BsmI BstEII BstZ17I CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PsiI PspXI PsrI SanDI SexAI SnaBI
SspI SwaI XhoI XmnI