Restriction map for
pF1KB8491
(Circular) (Six-base) MAPSORT of: pF1KB8491.seq Check: 2255 from: 1 to: 5822
pF1KB8491 5822 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1644 1644
Size: 5822
AccI GT'mk_AC
Cuts at: 2294 2860 2294
Size: 566 5256
Acc65I G'GTAC_C
Cuts at: 2838 2838
Size: 5822
AclI AA'CG_TT
Cuts at: 5662 5662
Size: 5822
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 1954 2620 3647 4079 4826 5094 224
Size: 1730 666 1027 432 747 268 952
Fragments arranged by size:
1730 1027 952 747 666 432 268
AflII C'TTAA_G
Cuts at: 1213 1213
Size: 5822
AflIII A'CryG_T
Cuts at: 3174 4278 5255 3174
Size: 1104 977 3741
Fragments arranged by size:
3741 1104 977
AgeI A'CCGG_T
Cuts at: 4210 4210
Size: 5822
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1016 1016
Size: 5822
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2647 2679 2647
Size: 32 5790
AlwNI CAG_nnn'CTG
Cuts at: 663 726 1233 1569 1593 1731 2067 2256 3127
Size: 63 507 336 24 138 336 189 871
Cuts at: 3127 4694 663
Size: 1567 1791
Fragments arranged by size:
1791 1567 871 507 336 336 189 138
63 24
ApaI G_GGCC'C
Cuts at: 1197 2035 1197
Size: 838 4984
ApaLI G'TGCA_C
Cuts at: 4592 4592
Size: 5822
ApoI r'AATT_y
Cuts at: 2238 2826 5175 5431 2238
Size: 588 2349 256 2629
Fragments arranged by size:
2629 2349 588 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5822
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5822
AvaI C'yCGr_G
Cuts at: 142 263 734 1118 1685 1867 2555 2781 2842
Size: 121 471 384 567 182 688 226 61
Cuts at: 2842 142
Size: 3122
Fragments arranged by size:
3122 688 567 471 384 226 182 121
61
BamHI G'GATC_C
Cuts at: 465 1439 2847 465
Size: 974 1408 3440
Fragments arranged by size:
3440 1408 974
BanI G'GyrC_C
Cuts at: 2771 2838 3252 3500 3535 2771
Size: 67 414 248 35 5058
Fragments arranged by size:
5058 414 248 67 35
BanII G_rGCy'C
Cuts at: 188 270 914 1197 1676 2035 2075 2571 2836
Size: 82 644 283 479 359 40 496 265
Cuts at: 2836 3866 188
Size: 1030 2144
Fragments arranged by size:
2144 1030 644 496 479 359 283 265
82 40
BbeI G_GCGC'C
Cuts at: 3256 3504 3256
Size: 248 5574
BbvCI CC'TCA_GC
Cuts at: 238 1661 2579 238
Size: 1423 918 3481
Fragments arranged by size:
3481 1423 918
BciVI GTATCCnnnnn_n'
Cuts at: 1359 3720 4481 5822 1359
Size: 2361 761 1341 1359
Fragments arranged by size:
2361 1359 1341 761
BclI T'GATC_A
Cuts at: 3342 3342
Size: 5822
BfrBI ATG'CAT
Cuts at: 673 673
Size: 5822
BglI GCCn_nnn'nGGC
Cuts at: 976 976
Size: 5822
BglII A'GATC_T
Cuts at: 2013 3337 5374 2013
Size: 1324 2037 2461
Fragments arranged by size:
2461 2037 1324
BlpI GC'TnA_GC
Cuts at: 579 860 1810 2219 2934 579
Size: 281 950 409 715 3467
Fragments arranged by size:
3467 950 715 409 281
Bme1580I G_kGCm'C
Cuts at: 1197 2035 2270 2774 3447 3540 4596 1197
Size: 838 235 504 673 93 1056 2423
Fragments arranged by size:
2423 1056 838 673 504 235 93
BmrI ACTGGGnnnn_n'
Cuts at: 125 1797 3034 3115 3450 5594 125
Size: 1672 1237 81 335 2144 353
Fragments arranged by size:
2144 1672 1237 353 335 81
BmtI G_CTAG'C
Cuts at: 1609 1609
Size: 5822
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1268 2454 2715 4189 1268
Size: 1186 261 1474 2901
Fragments arranged by size:
2901 1474 1186 261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 613 1054 1468 1492 1753 1845 2000 2089
Size: 204 441 414 24 261 92 155 89
Cuts at: 2089 2245 2278 2832 409
Size: 156 33 554 3399
Fragments arranged by size:
3399 554 441 414 261 204 156 155
92 89 33 24
Bpu10I CC'TnA_GC
Cuts at: 238 1505 1661 2397 2429 2579 238
Size: 1267 156 736 32 150 3481
Fragments arranged by size:
3481 1267 736 156 150 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1823 2218 2336 2998 3877 4369 4667 1823
Size: 395 118 662 879 492 298 2978
Fragments arranged by size:
2978 879 662 492 395 298 118
BsaI GGTCTCn'nnnn_
Cuts at: 1300 5526 1300
Size: 4226 1596
BsaAI yAC'GTr
Cuts at: 448 2362 3175 448
Size: 1914 813 3095
Fragments arranged by size:
3095 1914 813
BsaBI GATnn'nnATC
Cuts at: 6 3336 5373 6
Size: 3330 2037 455
Fragments arranged by size:
3330 2037 455
BsaHI Gr'CG_yC
Cuts at: 3253 3501 4203 5699 3253
Size: 248 702 1496 3376
Fragments arranged by size:
3376 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3011 3532 4210 4484 4631 5042 3011
Size: 521 678 274 147 411 3791
Fragments arranged by size:
3791 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 586 616 1899 1929 2647 2677 586
Size: 30 1283 30 718 30 3731
Fragments arranged by size:
3731 1283 718 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 610 1051 1255 1750 1835 1906 1911 1998 2430
Size: 441 204 495 85 71 5 87 432
Cuts at: 2430 2808 610
Size: 378 3624
Fragments arranged by size:
3624 495 441 432 378 204 87 85
71 5
BseYI C'CCAG_C
Cuts at: 215 269 1123 1196 1310 2101 2464 2561 3247
Size: 54 854 73 114 791 363 97 686
Cuts at: 3247 4005 4582 5078 215
Size: 758 577 496 959
Fragments arranged by size:
959 854 791 758 686 577 496 363
114 97 73 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1351 1435 1486 2099 1351
Size: 84 51 613 5074
Fragments arranged by size:
5074 613 84 51
BsiEI CG_ry'CG
Cuts at: 104 783 3364 3410 4618 104
Size: 679 2581 46 1208 1308
Fragments arranged by size:
2581 1308 1208 679 46
BsiHKAI G_wGCw'C
Cuts at: 914 1208 1676 2836 3614 3804 4596 914
Size: 294 468 1160 778 190 792 2140
Fragments arranged by size:
2140 1160 792 778 468 294 190
BsmI GAATG_Cn'
Cuts at: 2052 2052
Size: 5822
BsmBI CGTCTCn'nnnn_
Cuts at: 489 489
Size: 5822
Bsp1286I G_dGCh'C
Cuts at: 188 270 914 1197 1208 1676 2035 2075 2270
Size: 82 644 283 11 468 359 40 195
Cuts at: 2270 2571 2774 2836 3447 3540 3614 3804 3866
Size: 301 203 62 611 93 74 190 62
Cuts at: 3866 4596 188
Size: 730 1414
Fragments arranged by size:
1414 730 644 611 468 359 301 283
203 195 190 93 82 74 62 62
40 11
BspEI T'CCGG_A
Cuts at: 5042 5042
Size: 5822
BspHI T'CATG_A
Cuts at: 300 4998 300
Size: 4698 1124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1644 2872 3086 3388 3769 1644
Size: 1228 214 302 381 3697
Fragments arranged by size:
3697 1228 381 302 214
BsrBI CCG'CTC
Cuts at: 50 864 1107 1491 4114 4168 5652 5817 50
Size: 814 243 384 2623 54 1484 165 55
Fragments arranged by size:
2623 1484 814 384 243 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3734 3734
Size: 5822
BsrFI r'CCGG_y
Cuts at: 242 848 3820 4001 4210 242
Size: 606 2972 181 209 1854
Fragments arranged by size:
2972 1854 606 209 181
BssSI C'ACGA_G
Cuts at: 4093 4451 4093
Size: 358 5464
BstAPI GCAn_nnn'nTGC
Cuts at: 1773 2208 2253 3199 1773
Size: 435 45 946 4396
Fragments arranged by size:
4396 946 435 45
BstBI TT'CG_AA
Cuts at: 4183 4183
Size: 5822
BstXI CCAn_nnnn'nTGG
Cuts at: 1926 1926
Size: 5822
BstYI r'GATC_y
Cuts at: 1 153 465 1439 2013 2169 2583 2678 2847
Size: 152 312 974 574 156 414 95 169
Cuts at: 2847 3314 3337 3672 3918 4930 5016 5028 5374
Size: 467 23 335 246 1012 86 12 346
Cuts at: 5374 1
Size: 449
Fragments arranged by size:
1012 974 574 467 449 414 346 335
312 246 169 156 152 95 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 1537 1874 2162 1537
Size: 337 288 5197
Fragments arranged by size:
5197 337 288
BtgI C'CryG_G
Cuts at: 107 257 764 971 1919 1925 2402 107
Size: 150 507 207 948 6 477 3527
Fragments arranged by size:
3527 948 507 477 207 150 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3772 3953 4116 3772
Size: 181 163 5478
Fragments arranged by size:
5478 181 163
BtsI GCAGTG_nn'
Cuts at: 2463 2475 3065 2463
Size: 12 590 5220
Fragments arranged by size:
5220 590 12
DraI TTT'AAA
Cuts at: 2821 2821
Size: 5822
DrdI GACnn_nn'nnGTC
Cuts at: 3528 4386 3528
Size: 858 4964
EaeI y'GGCC_r
Cuts at: 625 1595 1922 2628 3407 3581 3972 3999 5344
Size: 970 327 706 779 174 391 27 1345
Cuts at: 5344 625
Size: 1103
Fragments arranged by size:
1345 1103 970 779 706 391 327 174
27
EagI C'GGCC_G
Cuts at: 3407 3407
Size: 5822
EarI CTCTTCn'nnn_
Cuts at: 497 1097 1163 1270 2596 3845 4055 497
Size: 600 66 107 1326 1249 210 2264
Fragments arranged by size:
2264 1326 1249 600 210 107 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 163 265 4030 4340 4486 5632 163
Size: 102 3765 310 146 1146 353
Fragments arranged by size:
3765 1146 353 310 146 102
EcoICRI GAG'CTC
Cuts at: 912 1674 2834 912
Size: 762 1160 3900
Fragments arranged by size:
3900 1160 762
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 409 613 1054 1468 1492 1753 1845 1954
Size: 185 204 441 414 24 261 92 109
Cuts at: 1954 2000 2089 2245 2278 2620 2832 3647 4079
Size: 46 89 156 33 342 212 815 432
Cuts at: 4079 4826 5094 224
Size: 747 268 952
Fragments arranged by size:
952 815 747 441 432 414 342 268
261 212 204 185 156 109 92 89
46 33 24
EcoNI CCTnn'n_nnAGG
Cuts at: 302 964 1536 302
Size: 662 572 4588
Fragments arranged by size:
4588 662 572
EcoO109I rG'GnC_Cy
Cuts at: 631 978 1566 2032 2105 2123 2190 2229 2424
Size: 347 588 466 73 18 67 39 195
Cuts at: 2424 2961 631
Size: 537 3492
Fragments arranged by size:
3492 588 537 466 347 195 73 67
39 18
EcoRI G'AATT_C
Cuts at: 2826 2826
Size: 5822
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 411 3841 411
Size: 3430 2392
FspI TGC'GCA
Cuts at: 3603 3603
Size: 5822
HaeII r_GCGC'y
Cuts at: 485 714 1234 1987 3256 3504 4526 5505 485
Size: 229 520 753 1269 248 1022 979 802
Fragments arranged by size:
1269 1022 979 802 753 520 248 229
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1125 1157 1899 1931 3664 3694 3696 3726 1125
Size: 32 742 32 1733 30 2 30 3221
Fragments arranged by size:
3221 1733 742 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2295 2861 2295
Size: 566 5256
HindIII A'AGCT_T
Cuts at: 339 339
Size: 5822
KasI G'GCGC_C
Cuts at: 3252 3500 3252
Size: 248 5574
KpnI G_GTAC'C
Cuts at: 2842 2842
Size: 5822
MluI A'CGCG_T
Cuts at: 5255 5255
Size: 5822
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 330 1046 1130 1238 1433 4493 4677 330
Size: 716 84 108 195 3060 184 1475
Fragments arranged by size:
3060 1475 716 195 184 108 84
MscI TGG'CCA
Cuts at: 1597 3583 1597
Size: 1986 3836
MslI CAynn'nnrTG
Cuts at: 1790 1924 3811 3938 1790
Size: 134 1887 127 3674
Fragments arranged by size:
3674 1887 134 127
MspA1I CmG'CkG
Cuts at: 121 273 1083 1398 1437 1446 1470 1479 1560
Size: 152 810 315 39 9 24 9 81
Cuts at: 1560 1731 1743 1806 2101 2223 2517 2618 2933
Size: 171 12 63 295 122 294 101 315
Cuts at: 2933 3247 3607 4620 4865 121
Size: 314 360 1013 245 1078
Fragments arranged by size:
1078 1013 810 360 315 315 314 295
294 245 171 152 122 101 81 63
39 24 12 9 9
NaeI GCC'GGC
Cuts at: 244 4003 244
Size: 3759 2063
NarI GG'CG_CC
Cuts at: 3253 3501 3253
Size: 248 5574
NcoI C'CATG_G
Cuts at: 107 971 1919 2402 107
Size: 864 948 483 3527
Fragments arranged by size:
3527 948 864 483
NgoMIV G'CCGG_C
Cuts at: 242 4001 242
Size: 3759 2063
NheI G'CTAG_C
Cuts at: 1605 1605
Size: 5822
NsiI A_TGCA'T
Cuts at: 675 675
Size: 5822
NspI r_CATG'y
Cuts at: 2875 3375 4282 2875
Size: 500 907 4415
Fragments arranged by size:
4415 907 500
PasI CC'CwG_GG
Cuts at: 181 181
Size: 5822
PciI A'CATG_T
Cuts at: 4278 4278
Size: 5822
PflMI CCAn_nnn'nTGG
Cuts at: 591 1731 2067 2256 591
Size: 1140 336 189 4157
Fragments arranged by size:
4157 1140 336 189
PfoI T'CCnGG_A
Cuts at: 2172 2172
Size: 5822
PmeI GTTT'AAAC
Cuts at: 2821 2821
Size: 5822
PmlI CAC'GTG
Cuts at: 448 448
Size: 5822
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2647 2679 4961 4993 2647
Size: 32 2282 32 3476
Fragments arranged by size:
3476 2282 32 32
PpuMI rG'GwC_Cy
Cuts at: 1566 2424 1566
Size: 858 4964
PspOMI G'GGCC_C
Cuts at: 1193 2031 1193
Size: 838 4984
PstI C_TGCA'G
Cuts at: 731 1013 1100 1238 1397 1565 1715 1769 1781
Size: 282 87 138 159 168 150 54 12
Cuts at: 1781 2869 3554 731
Size: 1088 685 2999
Fragments arranged by size:
2999 1088 685 282 168 159 150 138
87 54 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5822
PvuII CAG'CTG
Cuts at: 121 273 1398 1446 1470 1560 1731 1806 2101
Size: 152 1125 48 24 90 171 75 295
Cuts at: 2101 2223 2517 3247 3607 121
Size: 122 294 730 360 2336
Fragments arranged by size:
2336 1125 730 360 295 294 171 152
122 90 75 48 24
RsrII CG'GwC_CG
Cuts at: 4017 4017
Size: 5822
SacI G_AGCT'C
Cuts at: 914 1676 2836 914
Size: 762 1160 3900
Fragments arranged by size:
3900 1160 762
SalI G'TCGA_C
Cuts at: 2293 2859 2293
Size: 566 5256
SanDI GG'GwC_CC
Cuts at: 2424 2424
Size: 5822
SapI GCTCTTCn'nnn_
Cuts at: 1097 1270 3845 4055 1097
Size: 173 2575 210 2864
Fragments arranged by size:
2864 2575 210 173
SbfI CC_TGCA'GG
Cuts at: 1100 2869 1100
Size: 1769 4053
ScaI AGT'ACT
Cuts at: 5339 5339
Size: 5822
SfcI C'TryA_G
Cuts at: 33 727 748 1009 1096 1234 1393 1561 1711
Size: 694 21 261 87 138 159 168 150
Cuts at: 1711 1765 1777 2726 2865 3550 4543 4734 33
Size: 54 12 949 139 685 993 191 1121
Fragments arranged by size:
1121 993 949 694 685 261 191 168
159 150 139 138 87 54 21 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 976 976
Size: 5822
SfoI GGC'GCC
Cuts at: 3254 3502 3254
Size: 248 5574
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5822
SmaI CCC'GGG
Cuts at: 1687 1869 2844 1687
Size: 182 975 4665
Fragments arranged by size:
4665 975 182
SmlI C'TyrA_G
Cuts at: 1213 1838 2233 2351 2977 3892 4384 4646 1213
Size: 625 395 118 626 915 492 262 2389
Fragments arranged by size:
2389 915 626 625 492 395 262 118
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5822
SphI G_CATG'C
Cuts at: 2875 3375 2875
Size: 500 5322
StyI C'CwwG_G
Cuts at: 107 971 1919 2402 2509 2956 3310 107
Size: 864 948 483 107 447 354 2619
Fragments arranged by size:
2619 948 864 483 447 354 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2662 3405 3538 3997 4206 2662
Size: 743 133 459 209 4278
Fragments arranged by size:
4278 743 459 209 133
TatI w'GTAC_w
Cuts at: 5337 5337
Size: 5822
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2360 2392 2751 2783 5339 5371 2360
Size: 32 359 32 2556 32 2811
Fragments arranged by size:
2811 2556 359 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3619 3619
Size: 5822
XbaI T'CTAG_A
Cuts at: 2853 2853
Size: 5822
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3317 3317
Size: 5822
XmaI C'CCGG_G
Cuts at: 1685 1867 2842 1685
Size: 182 975 4665
Fragments arranged by size:
4665 975 182
XmnI GAAnn'nnTTC
Cuts at: 410 410
Size: 5822
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AscI AvrII BaeI BbsI
BcgI BmgBI BsiWI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HpaI MfeI NdeI
NotI NruI PacI PshAI PsiI PspXI PsrI SacII
SexAI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI