Restriction map for
pF1KB8485
(Circular) (Six-base) MAPSORT of: pF1KB8485.seq Check: 7592 from: 1 to: 5882
pF1KB8485 5882 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 836 2920 836
Size: 2084 3798
Acc65I G'GTAC_C
Cuts at: 2898 2898
Size: 5882
AclI AA'CG_TT
Cuts at: 5722 5722
Size: 5882
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 585 921 2437 3707 4139 4886 5154 110
Size: 475 336 1516 1270 432 747 268 838
Fragments arranged by size:
1516 1270 838 747 475 432 336 268
AflIII A'CryG_T
Cuts at: 851 1158 1767 3234 4338 5315 851
Size: 307 609 1467 1104 977 1418
Fragments arranged by size:
1467 1418 1104 977 609 307
AgeI A'CCGG_T
Cuts at: 4270 4270
Size: 5882
AhdI GACnn_n'nnGTC
Cuts at: 996 2319 996
Size: 1323 4559
AleI CACnn'nnGTG
Cuts at: 1268 1780 1268
Size: 512 5370
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1286 1441 2022 1286
Size: 155 581 5146
Fragments arranged by size:
5146 581 155
AlwNI CAG_nnn'CTG
Cuts at: 291 588 711 1415 1896 2695 3187 4754 291
Size: 297 123 704 481 799 492 1567 1419
Fragments arranged by size:
1567 1419 799 704 492 481 297 123
ApaI G_GGCC'C
Cuts at: 185 185
Size: 5882
ApaLI G'TGCA_C
Cuts at: 1281 4652 1281
Size: 3371 2511
ApoI r'AATT_y
Cuts at: 2886 5235 5491 2886
Size: 2349 256 3277
Fragments arranged by size:
3277 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5882
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5882
AvaI C'yCGr_G
Cuts at: 178 1735 1813 2902 178
Size: 1557 78 1089 3158
Fragments arranged by size:
3158 1557 1089 78
BamHI G'GATC_C
Cuts at: 2907 2907
Size: 5882
BanI G'GyrC_C
Cuts at: 781 844 1403 1547 2167 2254 2898 3312 3560
Size: 63 559 144 620 87 644 414 248
Cuts at: 3560 3595 781
Size: 35 3068
Fragments arranged by size:
3068 644 620 559 414 248 144 87
63 35
BanII G_rGCy'C
Cuts at: 185 467 934 1234 1643 2195 2896 3926 185
Size: 282 467 300 409 552 701 1030 2141
Fragments arranged by size:
2141 1030 701 552 467 409 300 282
BbeI G_GCGC'C
Cuts at: 1551 2258 3316 3564 1551
Size: 707 1058 248 3869
Fragments arranged by size:
3869 1058 707 248
BbsI GAAGACnn'nnnn_
Cuts at: 116 902 116
Size: 786 5096
BbvCI CC'TCA_GC
Cuts at: 292 292
Size: 5882
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 739 773 2851 2885 739
Size: 34 2078 34 3736
Fragments arranged by size:
3736 2078 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3780 4541 5882 3780
Size: 761 1341 3780
Fragments arranged by size:
3780 1341 761
BclI T'GATC_A
Cuts at: 1590 2357 3402 1590
Size: 767 1045 4070
Fragments arranged by size:
4070 1045 767
BglI GCCn_nnn'nGGC
Cuts at: 406 1315 1534 406
Size: 909 219 4754
Fragments arranged by size:
4754 909 219
BglII A'GATC_T
Cuts at: 1799 3397 5434 1799
Size: 1598 2037 2247
Fragments arranged by size:
2247 2037 1598
BlpI GC'TnA_GC
Cuts at: 1132 2287 2994 1132
Size: 1155 707 4020
Fragments arranged by size:
4020 1155 707
Bme1580I G_kGCm'C
Cuts at: 185 243 784 847 1285 1744 3507 3600 4656
Size: 58 541 63 438 459 1763 93 1056
Cuts at: 4656 185
Size: 1411
Fragments arranged by size:
1763 1411 1056 541 459 438 93 63
58
BmgBI CAC'GTC
Cuts at: 753 1266 753
Size: 513 5369
BmrI ACTGGGnnnn_n'
Cuts at: 1196 1927 3094 3175 3510 5654 1196
Size: 731 1167 81 335 2144 1424
Fragments arranged by size:
2144 1424 1167 731 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 948 1197 1674 1821 4249 948
Size: 249 477 147 2428 2581
Fragments arranged by size:
2581 2428 477 249 147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 1321 1348 1576 2724 409
Size: 912 27 228 1148 3567
Fragments arranged by size:
3567 1148 912 228 27
Bpu10I CC'TnA_GC
Cuts at: 292 1228 1897 292
Size: 936 669 4277
Fragments arranged by size:
4277 936 669
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3058 3937 4429 4727 3058
Size: 879 492 298 4213
Fragments arranged by size:
4213 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1555 5586 1555
Size: 4031 1851
BsaAI yAC'GTr
Cuts at: 342 1161 1768 3235 342
Size: 819 607 1467 2989
Fragments arranged by size:
2989 1467 819 607
BsaBI GATnn'nnATC
Cuts at: 6 3396 5433 6
Size: 3390 2037 455
Fragments arranged by size:
3390 2037 455
BsaHI Gr'CG_yC
Cuts at: 1548 2255 2320 2570 3313 3561 4263 5759 1548
Size: 707 65 250 743 248 702 1496 1671
Fragments arranged by size:
1671 1496 743 707 702 250 248 65
BsaWI w'CCGG_w
Cuts at: 323 1381 1577 2195 3071 3592 4270 4544 4691
Size: 1058 196 618 876 521 678 274 147
Cuts at: 4691 5102 323
Size: 411 1103
Fragments arranged by size:
1103 1058 876 678 618 521 411 274
196 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 365 395 1346 1376 1406 365
Size: 30 951 30 30 4841
Fragments arranged by size:
4841 951 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 880 1573 1647 2203 181
Size: 699 693 74 556 3860
Fragments arranged by size:
3860 699 693 556 74
BseYI C'CCAG_C
Cuts at: 393 504 954 1295 2618 3307 4065 4642 5138
Size: 111 450 341 1323 689 758 577 496
Cuts at: 5138 393
Size: 1137
Fragments arranged by size:
1323 1137 758 689 577 496 450 341
111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 696 1098 1279 1804 2710 226
Size: 470 402 181 525 906 3398
Fragments arranged by size:
3398 906 525 470 402 181
BsiEI CG_ry'CG
Cuts at: 104 1277 3424 3470 4678 104
Size: 1173 2147 46 1208 1308
Fragments arranged by size:
2147 1308 1208 1173 46
BsiHKAI G_wGCw'C
Cuts at: 646 1017 1285 2195 2896 3674 3864 4656 646
Size: 371 268 910 701 778 190 792 1872
Fragments arranged by size:
1872 910 792 778 701 371 268 190
BsmI GAATG_Cn'
Cuts at: 1462 1462
Size: 5882
Bsp1286I G_dGCh'C
Cuts at: 185 243 467 646 784 847 934 1017 1234
Size: 58 224 179 138 63 87 83 217
Cuts at: 1234 1285 1643 1744 2195 2896 3507 3600 3674
Size: 51 358 101 451 701 611 93 74
Cuts at: 3674 3864 3926 4656 185
Size: 190 62 730 1411
Fragments arranged by size:
1411 730 701 611 451 358 224 217
190 179 138 101 93 87 83 74
63 62 58 51
BspEI T'CCGG_A
Cuts at: 323 2195 5102 323
Size: 1872 2907 1103
Fragments arranged by size:
2907 1872 1103
BspHI T'CATG_A
Cuts at: 5058 5058
Size: 5882
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2511 2932 3146 3448 3829 2511
Size: 421 214 302 381 4564
Fragments arranged by size:
4564 421 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1401 1901 4174 4228 5712 5877 50
Size: 1351 500 2273 54 1484 165 55
Fragments arranged by size:
2273 1484 1351 500 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3794 3794
Size: 5882
BsrFI r'CCGG_y
Cuts at: 1544 2164 3880 4061 4270 1544
Size: 620 1716 181 209 3156
Fragments arranged by size:
3156 1716 620 209 181
BssSI C'ACGA_G
Cuts at: 2870 4153 4511 2870
Size: 1283 358 4241
Fragments arranged by size:
4241 1283 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3259 3259
Size: 5882
BstBI TT'CG_AA
Cuts at: 4243 4243
Size: 5882
BstEII G'GTnAC_C
Cuts at: 2835 2835
Size: 5882
BstXI CCAn_nnnn'nTGG
Cuts at: 270 1834 2626 2748 270
Size: 1564 792 122 3404
Fragments arranged by size:
3404 1564 792 122
BstYI r'GATC_y
Cuts at: 1 254 1799 2441 2541 2657 2907 3374 3397
Size: 253 1545 642 100 116 250 467 23
Cuts at: 3397 3732 3978 4990 5076 5088 5434 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1545 1012 642 467 449 346 335 253
250 246 116 100 86 23 12
BtgI C'CryG_G
Cuts at: 107 278 1520 1628 2645 107
Size: 171 1242 108 1017 3344
Fragments arranged by size:
3344 1242 1017 171 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 956 1475 1522 3832 4013 4176 956
Size: 519 47 2310 181 163 2662
Fragments arranged by size:
2662 2310 519 181 163 47
BtsI GCAGTG_nn'
Cuts at: 412 540 684 890 2069 2696 3125 412
Size: 128 144 206 1179 627 429 3169
Fragments arranged by size:
3169 1179 627 429 206 144 128
DraI TTT'AAA
Cuts at: 2881 2881
Size: 5882
DraIII CAC_nnn'GTG
Cuts at: 418 1860 418
Size: 1442 4440
DrdI GACnn_nn'nnGTC
Cuts at: 2112 3588 4446 2112
Size: 1476 858 3548
Fragments arranged by size:
3548 1476 858
EaeI y'GGCC_r
Cuts at: 513 1274 1316 1376 1494 1631 2279 2753 3467
Size: 761 42 60 118 137 648 474 714
Cuts at: 3467 3641 4032 4059 5404 513
Size: 174 391 27 1345 991
Fragments arranged by size:
1345 991 761 714 648 474 391 174
137 118 60 42 27
EagI C'GGCC_G
Cuts at: 1274 3467 1274
Size: 2193 3689
EarI CTCTTCn'nnn_
Cuts at: 2431 2675 3905 4115 2431
Size: 244 1230 210 4198
Fragments arranged by size:
4198 1230 244 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2377 4090 4400 4546 5692 2377
Size: 1713 310 146 1146 2567
Fragments arranged by size:
2567 1713 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2193 2894 2193
Size: 701 5181
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 409 585 921 1321 1348 1576 2437 2724
Size: 299 176 336 400 27 228 861 287
Cuts at: 2724 3707 4139 4886 5154 110
Size: 983 432 747 268 838
Fragments arranged by size:
983 861 838 747 432 400 336 299
287 268 228 176 27
EcoNI CCTnn'n_nnAGG
Cuts at: 1555 1960 2518 1555
Size: 405 558 4919
Fragments arranged by size:
4919 558 405
EcoO109I rG'GnC_Cy
Cuts at: 182 407 737 1098 1176 1893 2130 2673 3021
Size: 225 330 361 78 717 237 543 348
Cuts at: 3021 182
Size: 3043
Fragments arranged by size:
3043 717 543 361 348 330 237 225
78
EcoRI G'AATT_C
Cuts at: 2886 2886
Size: 5882
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3901 3901
Size: 5882
FspI TGC'GCA
Cuts at: 566 2861 3663 566
Size: 2295 802 2785
Fragments arranged by size:
2785 2295 802
HaeII r_GCGC'y
Cuts at: 292 955 1551 2258 2451 3316 3564 4586 5565
Size: 663 596 707 193 865 248 1022 979
Cuts at: 5565 292
Size: 609
Fragments arranged by size:
1022 979 865 707 663 609 596 248
193
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 982 1014 2257 2289 2305 2337 2797 2829 3724
Size: 32 1243 32 16 32 460 32 895
Cuts at: 3724 3754 3756 3786 982
Size: 30 2 30 3078
Fragments arranged by size:
3078 1243 895 460 32 32 32 32
30 30 16 2
HincII GTy'rAC
Cuts at: 2867 2921 2867
Size: 54 5828
KasI G'GCGC_C
Cuts at: 1547 2254 3312 3560 1547
Size: 707 1058 248 3869
Fragments arranged by size:
3869 1058 707 248
KpnI G_GTAC'C
Cuts at: 2902 2902
Size: 5882
MluI A'CGCG_T
Cuts at: 851 5315 851
Size: 4464 1418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2139 4553 4737 2139
Size: 2414 184 3284
Fragments arranged by size:
3284 2414 184
MscI TGG'CCA
Cuts at: 515 1378 1633 2281 3643 515
Size: 863 255 648 1362 2754
Fragments arranged by size:
2754 1362 863 648 255
MslI CAynn'nnrTG
Cuts at: 751 787 1268 1780 2242 3871 3998 751
Size: 36 481 512 462 1629 127 2635
Fragments arranged by size:
2635 1629 512 481 462 127 36
MspA1I CmG'CkG
Cuts at: 708 1321 2363 2842 2993 3307 3667 4680 4925
Size: 613 1042 479 151 314 360 1013 245
Cuts at: 4925 708
Size: 1665
Fragments arranged by size:
1665 1042 1013 613 479 360 314 245
151
NaeI GCC'GGC
Cuts at: 4063 4063
Size: 5882
NarI GG'CG_CC
Cuts at: 1548 2255 3313 3561 1548
Size: 707 1058 248 3869
Fragments arranged by size:
3869 1058 707 248
NcoI C'CATG_G
Cuts at: 107 278 1628 107
Size: 171 1350 4361
Fragments arranged by size:
4361 1350 171
NgoMIV G'CCGG_C
Cuts at: 4061 4061
Size: 5882
NspI r_CATG'y
Cuts at: 880 1671 2235 2370 2935 3435 4342 880
Size: 791 564 135 565 500 907 2420
Fragments arranged by size:
2420 907 791 565 564 500 135
PasI CC'CwG_GG
Cuts at: 1234 1827 1234
Size: 593 5289
PciI A'CATG_T
Cuts at: 4338 4338
Size: 5882
PflMI CCAn_nnn'nTGG
Cuts at: 699 987 1860 699
Size: 288 873 4721
Fragments arranged by size:
4721 873 288
PfoI T'CCnGG_A
Cuts at: 557 655 1151 2660 557
Size: 98 496 1509 3779
Fragments arranged by size:
3779 1509 496 98
PmeI GTTT'AAAC
Cuts at: 2881 2881
Size: 5882
PmlI CAC'GTG
Cuts at: 1161 1161
Size: 5882
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5021 5053 5021
Size: 32 5850
PpuMI rG'GwC_Cy
Cuts at: 1098 1176 2130 1098
Size: 78 954 4850
Fragments arranged by size:
4850 954 78
PshAI GACnn'nnGTC
Cuts at: 2872 2872
Size: 5882
PspOMI G'GGCC_C
Cuts at: 181 181
Size: 5882
PstI C_TGCA'G
Cuts at: 221 593 1217 1420 1430 2801 2929 3614 221
Size: 372 624 203 10 1371 128 685 2489
Fragments arranged by size:
2489 1371 685 624 372 203 128 10
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5882
PvuII CAG'CTG
Cuts at: 708 3307 3667 708
Size: 2599 360 2923
Fragments arranged by size:
2923 2599 360
RsrII CG'GwC_CG
Cuts at: 321 4077 321
Size: 3756 2126
SacI G_AGCT'C
Cuts at: 2195 2896 2195
Size: 701 5181
SalI G'TCGA_C
Cuts at: 2919 2919
Size: 5882
SapI GCTCTTCn'nnn_
Cuts at: 2675 3905 4115 2675
Size: 1230 210 4442
Fragments arranged by size:
4442 1230 210
SbfI CC_TGCA'GG
Cuts at: 1217 1430 2929 1217
Size: 213 1499 4170
Fragments arranged by size:
4170 1499 213
ScaI AGT'ACT
Cuts at: 5399 5399
Size: 5882
SfcI C'TryA_G
Cuts at: 33 217 247 589 1213 1416 1426 2797 2925
Size: 184 30 342 624 203 10 1371 128
Cuts at: 2925 3610 4603 4794 33
Size: 685 993 191 1121
Fragments arranged by size:
1371 1121 993 685 624 342 203 191
184 128 30 10
SfiI GGCCn_nnn'nGGCC
Cuts at: 406 406
Size: 5882
SfoI GGC'GCC
Cuts at: 1549 2256 3314 3562 1549
Size: 707 1058 248 3869
Fragments arranged by size:
3869 1058 707 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5882
SgrAI Cr'CCGG_yG
Cuts at: 1544 1544
Size: 5882
SmaI CCC'GGG
Cuts at: 180 2904 180
Size: 2724 3158
SmlI C'TyrA_G
Cuts at: 3037 3952 4444 4706 3037
Size: 915 492 262 4213
Fragments arranged by size:
4213 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5882
SphI G_CATG'C
Cuts at: 1671 2935 3435 1671
Size: 1264 500 4118
Fragments arranged by size:
4118 1264 500
SrfI GCCC'GGGC
Cuts at: 180 180
Size: 5882
StuI AGG'CCT
Cuts at: 1043 1654 2150 1043
Size: 611 496 4775
Fragments arranged by size:
4775 611 496
StyI C'CwwG_G
Cuts at: 107 278 1137 1628 1634 1985 2415 3016 3370
Size: 171 859 491 6 351 430 601 354
Cuts at: 3370 107
Size: 2619
Fragments arranged by size:
2619 859 601 491 430 354 351 171
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 417 496 1042 3465 3598 4057 4266 417
Size: 79 546 2423 133 459 209 2033
Fragments arranged by size:
2423 2033 546 459 209 133 79
TatI w'GTAC_w
Cuts at: 5397 5397
Size: 5882
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 365 397 1376 1408 5399 5431 365
Size: 32 979 32 3991 32 816
Fragments arranged by size:
3991 979 816 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3679 3679
Size: 5882
XbaI T'CTAG_A
Cuts at: 2913 2913
Size: 5882
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3377 3377
Size: 5882
XmaI C'CCGG_G
Cuts at: 178 2902 178
Size: 2724 3158
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI AscI AvrII BaeI
BfrBI BmtI BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PsiI PspXI
PsrI SacII SanDI SexAI SnaBI SspI SwaI XhoI
XmnI ZraI