Restriction map for
pF1KB8481
(Circular) (Six-base) MAPSORT of: pF1KB8481.seq Check: 139 from: 1 to: 5861
pF1KB8481 5861 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 1378 2794 2899 1378
Size: 1416 105 4340
Fragments arranged by size:
4340 1416 105
Acc65I G'GTAC_C
Cuts at: 2877 2877
Size: 5861
AclI AA'CG_TT
Cuts at: 5701 5701
Size: 5861
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 321 913 937 1631 1682 1847 1853 3686
Size: 159 592 24 694 51 165 6 1833
Cuts at: 3686 4118 4865 5133 162
Size: 432 747 268 890
Fragments arranged by size:
1833 890 747 694 592 432 268 165
159 51 24 6
AfeI AGC'GCT
Cuts at: 670 670
Size: 5861
AflII C'TTAA_G
Cuts at: 1444 1444
Size: 5861
AflIII A'CryG_T
Cuts at: 116 551 3213 4317 5294 116
Size: 435 2662 1104 977 683
Fragments arranged by size:
2662 1104 977 683 435
AgeI A'CCGG_T
Cuts at: 4249 4249
Size: 5861
AlwNI CAG_nnn'CTG
Cuts at: 429 513 786 1203 1825 3166 4733 429
Size: 84 273 417 622 1341 1567 1557
Fragments arranged by size:
1567 1557 1341 622 417 273 84
ApaLI G'TGCA_C
Cuts at: 463 2371 4631 463
Size: 1908 2260 1693
Fragments arranged by size:
2260 1908 1693
ApoI r'AATT_y
Cuts at: 1459 2865 5214 5470 1459
Size: 1406 2349 256 1850
Fragments arranged by size:
2349 1850 1406 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5861
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5861
AvaI C'yCGr_G
Cuts at: 171 2881 171
Size: 2710 3151
BamHI G'GATC_C
Cuts at: 1762 2488 2886 1762
Size: 726 398 4737
Fragments arranged by size:
4737 726 398
BanI G'GyrC_C
Cuts at: 1070 1114 2479 2877 3291 3539 3574 1070
Size: 44 1365 398 414 248 35 3357
Fragments arranged by size:
3357 1365 414 398 248 44 35
BanII G_rGCy'C
Cuts at: 2620 2875 3905 2620
Size: 255 1030 4576
Fragments arranged by size:
4576 1030 255
BbeI G_GCGC'C
Cuts at: 1074 3295 3543 1074
Size: 2221 248 3392
Fragments arranged by size:
3392 2221 248
BbsI GAAGACnn'nnnn_
Cuts at: 653 901 2507 653
Size: 248 1606 4007
Fragments arranged by size:
4007 1606 248
BbvCI CC'TCA_GC
Cuts at: 1174 1174
Size: 5861
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 258 292 424 458 717 751 258
Size: 34 132 34 259 34 5368
Fragments arranged by size:
5368 259 132 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3759 4520 5861 3759
Size: 761 1341 3759
Fragments arranged by size:
3759 1341 761
BclI T'GATC_A
Cuts at: 3381 3381
Size: 5861
BglII A'GATC_T
Cuts at: 3376 5413 3376
Size: 2037 3824
BlpI GC'TnA_GC
Cuts at: 310 1141 2973 310
Size: 831 1832 3198
Fragments arranged by size:
3198 1832 831
Bme1580I G_kGCm'C
Cuts at: 467 1225 2000 2039 2375 3486 3579 4635 467
Size: 758 775 39 336 1111 93 1056 1693
Fragments arranged by size:
1693 1111 1056 775 758 336 93 39
BmrI ACTGGGnnnn_n'
Cuts at: 1376 3073 3154 3489 5633 1376
Size: 1697 81 335 2144 1604
Fragments arranged by size:
2144 1697 1604 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 186 4228 186
Size: 4042 1819
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 610 661 1150 1342 1625 493
Size: 117 51 489 192 283 4729
Fragments arranged by size:
4729 489 283 192 117 51
Bpu10I CC'TnA_GC
Cuts at: 614 1174 614
Size: 560 5301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 2634 3037 3916 4408 4706 646
Size: 1988 403 879 492 298 1801
Fragments arranged by size:
1988 1801 879 492 403 298
BsaI GGTCTCn'nnnn_
Cuts at: 157 2380 2545 2764 5565 157
Size: 2223 165 219 2801 453
Fragments arranged by size:
2801 2223 453 219 165
BsaAI yAC'GTr
Cuts at: 3214 3214
Size: 5861
BsaBI GATnn'nnATC
Cuts at: 6 3375 5412 6
Size: 3369 2037 455
Fragments arranged by size:
3369 2037 455
BsaHI Gr'CG_yC
Cuts at: 745 1071 3292 3540 4242 5738 745
Size: 326 2221 248 702 1496 868
Fragments arranged by size:
2221 1496 868 702 326 248
BsaWI w'CCGG_w
Cuts at: 370 3050 3571 4249 4523 4670 5081 370
Size: 2680 521 678 274 147 411 1150
Fragments arranged by size:
2680 1150 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 352 382 673 703 352
Size: 30 291 30 5510
Fragments arranged by size:
5510 291 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 246 691 694 754 838 841 1168 1258 1483
Size: 445 3 60 84 3 327 90 225
Cuts at: 1483 1729 1918 2456 2473 2551 2596 2668 2794
Size: 246 189 538 17 78 45 72 126
Cuts at: 2794 246
Size: 3313
Fragments arranged by size:
3313 538 445 327 246 225 189 126
90 84 78 72 60 45 17 3
3
BseYI C'CCAG_C
Cuts at: 129 241 539 543 932 2619 3286 4044 4621
Size: 112 298 4 389 1687 667 758 577
Cuts at: 4621 5117 129
Size: 496 873
Fragments arranged by size:
1687 873 758 667 577 496 389 298
112 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 799 1640 640
Size: 159 841 4861
Fragments arranged by size:
4861 841 159
BsiEI CG_ry'CG
Cuts at: 104 438 1016 3403 3449 4657 104
Size: 334 578 2387 46 1208 1308
Fragments arranged by size:
2387 1308 1208 578 334 46
BsiHKAI G_wGCw'C
Cuts at: 467 2375 2875 3653 3843 4635 467
Size: 1908 500 778 190 792 1693
Fragments arranged by size:
1908 1693 792 778 500 190
BsiWI C'GTAC_G
Cuts at: 554 554
Size: 5861
Bsp1286I G_dGCh'C
Cuts at: 467 1225 2000 2039 2375 2620 2875 3486 3579
Size: 758 775 39 336 245 255 611 93
Cuts at: 3579 3653 3843 3905 4635 467
Size: 74 190 62 730 1693
Fragments arranged by size:
1693 775 758 730 611 336 255 245
190 93 74 62 39
BspEI T'CCGG_A
Cuts at: 370 5081 370
Size: 4711 1150
BspHI T'CATG_A
Cuts at: 5037 5037
Size: 5861
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2911 3125 3427 3808 2911
Size: 214 302 381 4964
Fragments arranged by size:
4964 381 302 214
BsrBI CCG'CTC
Cuts at: 50 170 215 401 929 1040 1134 4153 4207
Size: 120 45 186 528 111 94 3019 54
Cuts at: 4207 5691 5856 50
Size: 1484 165 55
Fragments arranged by size:
3019 1484 528 186 165 120 111 94
55 54 45
BsrDI GCAATG_nn'
Cuts at: 3773 3773
Size: 5861
BsrFI r'CCGG_y
Cuts at: 149 445 656 1067 3859 4040 4249 149
Size: 296 211 411 2792 181 209 1761
Fragments arranged by size:
2792 1761 411 296 211 209 181
BssHII G'CGCG_C
Cuts at: 579 719 579
Size: 140 5721
BssSI C'ACGA_G
Cuts at: 604 820 4132 4490 604
Size: 216 3312 358 1975
Fragments arranged by size:
3312 1975 358 216
BstAPI GCAn_nnn'nTGC
Cuts at: 786 3238 786
Size: 2452 3409
BstBI TT'CG_AA
Cuts at: 4222 4222
Size: 5861
BstEII G'GTnAC_C
Cuts at: 156 2346 2667 156
Size: 2190 321 3350
Fragments arranged by size:
3350 2190 321
BstYI r'GATC_y
Cuts at: 1 503 566 851 1762 2488 2886 3353 3376
Size: 502 63 285 911 726 398 467 23
Cuts at: 3376 3711 3957 4969 5055 5067 5413 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 911 726 502 467 449 398 346
335 285 246 86 63 23 12
BstZ17I GTA'TAC
Cuts at: 1379 1379
Size: 5861
BtgI C'CryG_G
Cuts at: 231 231
Size: 5861
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 845 3811 3992 4155 845
Size: 2966 181 163 2551
Fragments arranged by size:
2966 2551 181 163
BtsI GCAGTG_nn'
Cuts at: 2407 2574 3104 2407
Size: 167 530 5164
Fragments arranged by size:
5164 530 167
DraI TTT'AAA
Cuts at: 2860 2860
Size: 5861
DraIII CAC_nnn'GTG
Cuts at: 1229 1229
Size: 5861
DrdI GACnn_nn'nnGTC
Cuts at: 2509 3567 4425 2509
Size: 1058 858 3945
Fragments arranged by size:
3945 1058 858
EaeI y'GGCC_r
Cuts at: 302 710 833 1013 1547 3446 3620 4011 4038
Size: 408 123 180 534 1899 174 391 27
Cuts at: 4038 5383 302
Size: 1345 780
Fragments arranged by size:
1899 1345 780 534 408 391 180 174
123 27
EagI C'GGCC_G
Cuts at: 1013 3446 1013
Size: 2433 3428
EarI CTCTTCn'nnn_
Cuts at: 968 1046 1325 1511 1559 1589 1667 1706 1748
Size: 78 279 186 48 30 78 39 42
Cuts at: 1748 1970 2009 2048 2087 2126 2165 2246 2267
Size: 222 39 39 39 39 39 81 21
Cuts at: 2267 2390 2573 2753 2762 3884 4094 968
Size: 123 183 180 9 1122 210 2735
Fragments arranged by size:
2735 1122 279 222 210 186 183 180
123 81 78 78 48 42 39 39
39 39 39 39 30 21 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 132 306 514 1032 4069 4379 4525 5671 132
Size: 174 208 518 3037 310 146 1146 322
Fragments arranged by size:
3037 1146 518 322 310 208 174 146
EcoICRI GAG'CTC
Cuts at: 2873 2873
Size: 5861
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 321 493 610 661 913 937 1150 1342
Size: 159 172 117 51 252 24 213 192
Cuts at: 1342 1625 1631 1682 1847 1853 3686 4118 4865
Size: 283 6 51 165 6 1833 432 747
Cuts at: 4865 5133 162
Size: 268 890
Fragments arranged by size:
1833 890 747 432 283 268 252 213
192 172 165 159 117 51 51 24
6 6
EcoO109I rG'GnC_Cy
Cuts at: 1263 1521 3000 1263
Size: 258 1479 4124
Fragments arranged by size:
4124 1479 258
EcoRI G'AATT_C
Cuts at: 2865 2865
Size: 5861
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 687 2391 3880 687
Size: 1704 1489 2668
Fragments arranged by size:
2668 1704 1489
FspI TGC'GCA
Cuts at: 727 3642 727
Size: 2915 2946
HaeII r_GCGC'y
Cuts at: 514 672 916 1074 1161 3295 3543 4565 5544
Size: 158 244 158 87 2134 248 1022 979
Cuts at: 5544 514
Size: 831
Fragments arranged by size:
2134 1022 979 831 248 244 158 158
87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 730 762 1087 1119 1379 1411 1489 1521 1747
Size: 32 325 32 260 32 78 32 226
Cuts at: 1747 1779 2164 2196 3703 3733 3735 3765 730
Size: 32 385 32 1507 30 2 30 2826
Fragments arranged by size:
2826 1507 385 325 260 226 78 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2900 2900
Size: 5861
HindIII A'AGCT_T
Cuts at: 1441 1441
Size: 5861
KasI G'GCGC_C
Cuts at: 1070 3291 3539 1070
Size: 2221 248 3392
Fragments arranged by size:
3392 2221 248
KpnI G_GTAC'C
Cuts at: 2881 2881
Size: 5861
MluI A'CGCG_T
Cuts at: 551 5294 551
Size: 4743 1118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 101 428 1476 4532 4716 101
Size: 327 1048 3056 184 1246
Fragments arranged by size:
3056 1246 1048 327 184
MscI TGG'CCA
Cuts at: 3622 3622
Size: 5861
MslI CAynn'nnrTG
Cuts at: 871 3850 3977 871
Size: 2979 127 2755
Fragments arranged by size:
2979 2755 127
MspA1I CmG'CkG
Cuts at: 191 233 420 543 624 1077 1203 2972 3286
Size: 42 187 123 81 453 126 1769 314
Cuts at: 3286 3646 4659 4904 191
Size: 360 1013 245 1148
Fragments arranged by size:
1769 1148 1013 453 360 314 245 187
126 123 81 42
NaeI GCC'GGC
Cuts at: 447 4042 447
Size: 3595 2266
NarI GG'CG_CC
Cuts at: 1071 3292 3540 1071
Size: 2221 248 3392
Fragments arranged by size:
3392 2221 248
NgoMIV G'CCGG_C
Cuts at: 445 4040 445
Size: 3595 2266
NspI r_CATG'y
Cuts at: 276 2914 3414 4321 276
Size: 2638 500 907 1816
Fragments arranged by size:
2638 1816 907 500
PciI A'CATG_T
Cuts at: 4317 4317
Size: 5861
PflMI CCAn_nnn'nTGG
Cuts at: 1203 1203
Size: 5861
PfoI T'CCnGG_A
Cuts at: 1319 2491 1319
Size: 1172 4689
PmeI GTTT'AAAC
Cuts at: 2860 2860
Size: 5861
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5000 5032 5000
Size: 32 5829
PpuMI rG'GwC_Cy
Cuts at: 1263 1263
Size: 5861
PspXI vC'TCGA_Gb
Cuts at: 171 171
Size: 5861
PstI C_TGCA'G
Cuts at: 425 791 1082 2414 2908 3593 425
Size: 366 291 1332 494 685 2693
Fragments arranged by size:
2693 1332 685 494 366 291
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5861
PvuII CAG'CTG
Cuts at: 420 543 624 1077 1203 3286 3646 420
Size: 123 81 453 126 2083 360 2635
Fragments arranged by size:
2635 2083 453 360 126 123 81
RsrII CG'GwC_CG
Cuts at: 4056 4056
Size: 5861
SacI G_AGCT'C
Cuts at: 2875 2875
Size: 5861
SacII CC_GC'GG
Cuts at: 234 234
Size: 5861
SalI G'TCGA_C
Cuts at: 2898 2898
Size: 5861
SapI GCTCTTCn'nnn_
Cuts at: 2753 3884 4094 2753
Size: 1131 210 4520
Fragments arranged by size:
4520 1131 210
SbfI CC_TGCA'GG
Cuts at: 2908 2908
Size: 5861
ScaI AGT'ACT
Cuts at: 5378 5378
Size: 5861
SfcI C'TryA_G
Cuts at: 33 421 787 1078 2410 2904 3589 4582 4773
Size: 388 366 291 1332 494 685 993 191
Cuts at: 4773 33
Size: 1121
Fragments arranged by size:
1332 1121 993 685 494 388 366 291
191
SfoI GGC'GCC
Cuts at: 1072 3293 3541 1072
Size: 2221 248 3392
Fragments arranged by size:
3392 2221 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5861
SmaI CCC'GGG
Cuts at: 2883 2883
Size: 5861
SmlI C'TyrA_G
Cuts at: 171 661 1444 2613 3016 3931 4423 4685 171
Size: 490 783 1169 403 915 492 262 1347
Fragments arranged by size:
1347 1169 915 783 492 490 403 262
SpeI A'CTAG_T
Cuts at: 67 2787 67
Size: 2720 3141
SphI G_CATG'C
Cuts at: 276 2914 3414 276
Size: 2638 500 2723
Fragments arranged by size:
2723 2638 500
StuI AGG'CCT
Cuts at: 529 529
Size: 5861
StyI C'CwwG_G
Cuts at: 1046 1118 1424 1898 1922 2246 2483 2555 2995
Size: 72 306 474 24 324 237 72 440
Cuts at: 2995 3349 1046
Size: 354 3558
Fragments arranged by size:
3558 474 440 354 324 306 237 72
72 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2860 3444 3577 4036 4245 2860
Size: 584 133 459 209 4476
Fragments arranged by size:
4476 584 459 209 133
TatI w'GTAC_w
Cuts at: 5376 5376
Size: 5861
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5378 5410 5378
Size: 32 5829
Tth111I GACn'n_nGTC
Cuts at: 3658 3658
Size: 5861
XbaI T'CTAG_A
Cuts at: 2892 2892
Size: 5861
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 591 1125 1200 1905 2007 2046 3356 591
Size: 534 75 705 102 39 1310 3096
Fragments arranged by size:
3096 1310 705 534 102 75 39
XhoI C'TCGA_G
Cuts at: 171 171
Size: 5861
XmaI C'CCGG_G
Cuts at: 2881 2881
Size: 5861
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BsmI BsmBI
BsrGI BstXI Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PshAI PsiI PspOMI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XmnI ZraI