Restriction map for
pF1KB8478
(Circular) (Six-base) MAPSORT of: pF1KB8478.seq Check: 1201 from: 1 to: 5654
pF1KB8478 5654 bp
With 182 enzymes: SgfI *
March 2, 2011 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1153 1445 1485 2565 1153
Size: 292 40 1080 4242
Fragments arranged by size:
4242 1080 292 40
AatII G_ACGT'C
Cuts at: 1022 1761 1022
Size: 739 4915
AccI GT'mk_AC
Cuts at: 2692 2692
Size: 5654
Acc65I G'GTAC_C
Cuts at: 1877 2670 1877
Size: 793 4861
AclI AA'CG_TT
Cuts at: 5494 5494
Size: 5654
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 916 3479 3911 4658 4926 916
Size: 2563 432 747 268 1644
Fragments arranged by size:
2563 1644 747 432 268
AflIII A'CryG_T
Cuts at: 3006 4110 5087 3006
Size: 1104 977 3573
Fragments arranged by size:
3573 1104 977
AgeI A'CCGG_T
Cuts at: 4042 4042
Size: 5654
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1633 2074 1633
Size: 441 5213
AlwNI CAG_nnn'CTG
Cuts at: 456 2959 4526 456
Size: 2503 1567 1584
Fragments arranged by size:
2503 1584 1567
ApaI G_GGCC'C
Cuts at: 707 2581 707
Size: 1874 3780
ApaLI G'TGCA_C
Cuts at: 4424 4424
Size: 5654
ApoI r'AATT_y
Cuts at: 2658 5007 5263 2658
Size: 2349 256 3049
Fragments arranged by size:
3049 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5654
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5654
AvaI C'yCGr_G
Cuts at: 323 410 920 1150 1966 2674 323
Size: 87 510 230 816 708 3303
Fragments arranged by size:
3303 816 708 510 230 87
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2241 2274 2241
Size: 33 5621
BamHI G'GATC_C
Cuts at: 2679 2679
Size: 5654
BanI G'GyrC_C
Cuts at: 810 958 1065 1877 2050 2359 2368 2614 2631
Size: 148 107 812 173 309 9 246 17
Cuts at: 2631 2670 3084 3332 3367 810
Size: 39 414 248 35 3097
Fragments arranged by size:
3097 812 414 309 248 246 173 148
107 39 35 17 9
BanII G_rGCy'C
Cuts at: 116 142 269 668 707 720 1157 1967 1976
Size: 26 127 399 39 13 437 810 9
Cuts at: 1976 2290 2309 2581 2668 3698 116
Size: 314 19 272 87 1030 2072
Fragments arranged by size:
2072 1030 810 437 399 314 272 127
87 39 26 19 13 9
BbeI G_GCGC'C
Cuts at: 3088 3336 3088
Size: 248 5406
BbsI GAAGACnn'nnnn_
Cuts at: 1647 1647
Size: 5654
BbvCI CC'TCA_GC
Cuts at: 709 709
Size: 5654
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1575 1609 1575
Size: 34 5620
BciVI GTATCCnnnnn_n'
Cuts at: 2269 3552 4313 5654 2269
Size: 1283 761 1341 2269
Fragments arranged by size:
2269 1341 1283 761
BclI T'GATC_A
Cuts at: 3174 3174
Size: 5654
BglI GCCn_nnn'nGGC
Cuts at: 273 383 619 646 973 1980 273
Size: 110 236 27 327 1007 3947
Fragments arranged by size:
3947 1007 327 236 110 27
BglII A'GATC_T
Cuts at: 3169 5206 3169
Size: 2037 3617
BlpI GC'TnA_GC
Cuts at: 2766 2766
Size: 5654
Bme1580I G_kGCm'C
Cuts at: 707 815 961 2581 2598 2619 3279 3372 4428
Size: 108 146 1620 17 21 660 93 1056
Cuts at: 4428 707
Size: 1933
Fragments arranged by size:
1933 1620 1056 660 146 108 93 21
17
BmgBI CAC'GTC
Cuts at: 2296 2296
Size: 5654
BmrI ACTGGGnnnn_n'
Cuts at: 263 768 1440 1805 2866 2947 3282 5426 263
Size: 505 672 365 1061 81 335 2144 491
Fragments arranged by size:
2144 1061 672 505 491 365 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4021 4021
Size: 5654
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 679 1044 1264 1783 1981 2463 484
Size: 195 365 220 519 198 482 3675
Fragments arranged by size:
3675 519 482 365 220 198 195
Bpu10I CC'TnA_GC
Cuts at: 709 709
Size: 5654
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 628 817 1755 2191 2830 3709 4201 4499
Size: 510 189 938 436 639 879 492 298
Cuts at: 4499 118
Size: 1273
Fragments arranged by size:
1273 938 879 639 510 492 436 298
189
BsaI GGTCTCn'nnnn_
Cuts at: 215 1327 5358 215
Size: 1112 4031 511
Fragments arranged by size:
4031 1112 511
BsaAI yAC'GTr
Cuts at: 1518 3007 1518
Size: 1489 4165
BsaBI GATnn'nnATC
Cuts at: 6 3168 5205 6
Size: 3162 2037 455
Fragments arranged by size:
3162 2037 455
BsaHI Gr'CG_yC
Cuts at: 1019 1758 2017 2212 3085 3333 4035 5531 1019
Size: 739 259 195 873 248 702 1496 1142
Fragments arranged by size:
1496 1142 873 739 702 259 248 195
BsaWI w'CCGG_w
Cuts at: 1640 2843 3364 4042 4316 4463 4874 1640
Size: 1203 521 678 274 147 411 2420
Fragments arranged by size:
2420 1203 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 304 334 1201 1231 2306 2336 304
Size: 30 867 30 1075 30 3622
Fragments arranged by size:
3622 1075 867 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 206 481 676 1384 1387 1659 1860 1984 2314
Size: 275 195 708 3 272 201 124 330
Cuts at: 2314 2317 206
Size: 3 3543
Fragments arranged by size:
3543 708 330 275 272 201 195 124
3 3
BseYI C'CCAG_C
Cuts at: 1181 1796 1926 2070 2458 3079 3837 4414 4910
Size: 615 130 144 388 621 758 577 496
Cuts at: 4910 1181
Size: 1925
Fragments arranged by size:
1925 758 621 615 577 496 388 144
130
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 787 1639 2219 2480 778
Size: 9 852 580 261 3952
Fragments arranged by size:
3952 852 580 261 9
BsiEI CG_ry'CG
Cuts at: 104 1513 2171 3196 3242 4450 104
Size: 1409 658 1025 46 1208 1308
Fragments arranged by size:
1409 1308 1208 1025 658 46
BsiHKAI G_wGCw'C
Cuts at: 720 887 1436 2511 2668 3446 3636 4428 720
Size: 167 549 1075 157 778 190 792 1946
Fragments arranged by size:
1946 1075 792 778 549 190 167 157
BsmBI CGTCTCn'nnnn_
Cuts at: 338 338
Size: 5654
Bsp1286I G_dGCh'C
Cuts at: 116 142 269 668 707 720 815 887 961
Size: 26 127 399 39 13 95 72 74
Cuts at: 961 1157 1436 1967 1976 2290 2309 2511 2581
Size: 196 279 531 9 314 19 202 70
Cuts at: 2581 2598 2619 2668 3279 3372 3446 3636 3698
Size: 17 21 49 611 93 74 190 62
Cuts at: 3698 4428 116
Size: 730 1342
Fragments arranged by size:
1342 730 611 531 399 314 279 202
196 190 127 95 93 74 74 72
70 62 49 39 26 21 19 17
13 9
BspEI T'CCGG_A
Cuts at: 4874 4874
Size: 5654
BspHI T'CATG_A
Cuts at: 4830 4830
Size: 5654
BspMI ACCTGCnnnn'nnnn_
Cuts at: 800 1153 1445 1485 2096 2565 2704 2918 3220
Size: 353 292 40 611 469 139 214 302
Cuts at: 3220 3601 800
Size: 381 2853
Fragments arranged by size:
2853 611 469 381 353 302 292 214
139 40
BsrBI CCG'CTC
Cuts at: 50 1387 1785 2190 3946 4000 5484 5649 50
Size: 1337 398 405 1756 54 1484 165 55
Fragments arranged by size:
1756 1484 1337 405 398 165 55 54
BsrDI GCAATG_nn'
Cuts at: 635 1782 3566 635
Size: 1147 1784 2723
Fragments arranged by size:
2723 1784 1147
BsrFI r'CCGG_y
Cuts at: 197 697 3652 3833 4042 197
Size: 500 2955 181 209 1809
Fragments arranged by size:
2955 1809 500 209 181
BsrGI T'GTAC_A
Cuts at: 500 500
Size: 5654
BssHII G'CGCG_C
Cuts at: 1390 1561 1390
Size: 171 5483
BssSI C'ACGA_G
Cuts at: 3925 4283 3925
Size: 358 5296
BstAPI GCAn_nnn'nTGC
Cuts at: 792 1085 3031 792
Size: 293 1946 3415
Fragments arranged by size:
3415 1946 293
BstBI TT'CG_AA
Cuts at: 4015 4015
Size: 5654
BstEII G'GTnAC_C
Cuts at: 301 1939 301
Size: 1638 4016
BstXI CCAn_nnnn'nTGG
Cuts at: 373 1710 2610 373
Size: 1337 900 3417
Fragments arranged by size:
3417 1337 900
BstYI r'GATC_y
Cuts at: 1 1313 1909 2679 3146 3169 3504 3750 4762
Size: 1312 596 770 467 23 335 246 1012
Cuts at: 4762 4848 4860 5206 1
Size: 86 12 346 449
Fragments arranged by size:
1312 1012 770 596 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 203 292 980 1905 203
Size: 89 688 925 3952
Fragments arranged by size:
3952 925 688 89
BtgI C'CryG_G
Cuts at: 107 777 953 1074 1270 1530 1948 2310 2464
Size: 670 176 121 196 260 418 362 154
Cuts at: 2464 2626 107
Size: 162 3135
Fragments arranged by size:
3135 670 418 362 260 196 176 162
154 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3604 3785 3948 3604
Size: 181 163 5310
Fragments arranged by size:
5310 181 163
BtsI GCAGTG_nn'
Cuts at: 168 795 1020 1126 2897 168
Size: 627 225 106 1771 2925
Fragments arranged by size:
2925 1771 627 225 106
DraI TTT'AAA
Cuts at: 2653 2653
Size: 5654
DrdI GACnn_nn'nnGTC
Cuts at: 3360 4218 3360
Size: 858 4796
EaeI y'GGCC_r
Cuts at: 375 440 722 1172 2079 3239 3413 3804 3831
Size: 65 282 450 907 1160 174 391 27
Cuts at: 3831 5176 375
Size: 1345 853
Fragments arranged by size:
1345 1160 907 853 450 391 282 174
65 27
EagI C'GGCC_G
Cuts at: 3239 3239
Size: 5654
EarI CTCTTCn'nnn_
Cuts at: 115 133 613 1330 3677 3887 115
Size: 18 480 717 2347 210 1882
Fragments arranged by size:
2347 1882 717 480 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1426 3862 4172 4318 5464 1426
Size: 2436 310 146 1146 1616
Fragments arranged by size:
2436 1616 1146 310 146
EcoICRI GAG'CTC
Cuts at: 718 2666 718
Size: 1948 3706
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 679 916 1044 1264 1783 1981 2463 3479
Size: 195 237 128 220 519 198 482 1016
Cuts at: 3479 3911 4658 4926 484
Size: 432 747 268 1212
Fragments arranged by size:
1212 1016 747 519 482 432 268 237
220 198 195 128
EcoNI CCTnn'n_nnAGG
Cuts at: 295 295
Size: 5654
EcoO109I rG'GnC_Cy
Cuts at: 552 704 804 1191 1254 1807 1853 2577 2578
Size: 152 100 387 63 553 46 724 1
Cuts at: 2578 2622 2644 2793 552
Size: 44 22 149 3413
Fragments arranged by size:
3413 724 553 387 152 149 100 63
46 44 22 1
EcoRI G'AATT_C
Cuts at: 2658 2658
Size: 5654
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 144 3673 144
Size: 3529 2125
FseI GG_CCGG'CC
Cuts at: 201 201
Size: 5654
FspI TGC'GCA
Cuts at: 3435 3435
Size: 5654
HaeII r_GCGC'y
Cuts at: 1243 2041 3088 3336 4358 5337 1243
Size: 798 1047 248 1022 979 1560
Fragments arranged by size:
1560 1047 1022 979 798 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 304 336 820 852 1201 1233 1305 1337 1387
Size: 32 484 32 349 32 72 32 50
Cuts at: 1387 1419 1882 1914 2306 2338 3496 3526 3528
Size: 32 463 32 392 32 1158 30 2
Cuts at: 3528 3558 304
Size: 30 2400
Fragments arranged by size:
2400 1158 484 463 392 349 72 50
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 756 1116 2693 756
Size: 360 1577 3717
Fragments arranged by size:
3717 1577 360
HpaI GTT'AAC
Cuts at: 1116 1116
Size: 5654
KasI G'GCGC_C
Cuts at: 3084 3332 3084
Size: 248 5406
KpnI G_GTAC'C
Cuts at: 1881 2674 1881
Size: 793 4861
MluI A'CGCG_T
Cuts at: 5087 5087
Size: 5654
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 422 863 1214 1691 4325 4509 422
Size: 441 351 477 2634 184 1567
Fragments arranged by size:
2634 1567 477 441 351 184
MscI TGG'CCA
Cuts at: 377 442 3415 377
Size: 65 2973 2616
Fragments arranged by size:
2973 2616 65
MslI CAynn'nnrTG
Cuts at: 571 943 1615 3643 3770 571
Size: 372 672 2028 127 2455
Fragments arranged by size:
2455 2028 672 372 127
MspA1I CmG'CkG
Cuts at: 170 223 512 955 1272 1401 1419 1532 1950
Size: 53 289 443 317 129 18 113 418
Cuts at: 1950 2034 2274 2312 2458 2765 3079 3439 4452
Size: 84 240 38 146 307 314 360 1013
Cuts at: 4452 4697 170
Size: 245 1127
Fragments arranged by size:
1127 1013 443 418 360 317 314 307
289 245 240 146 129 113 84 53
38 18
NaeI GCC'GGC
Cuts at: 199 3835 199
Size: 3636 2018
NarI GG'CG_CC
Cuts at: 3085 3333 3085
Size: 248 5406
NcoI C'CATG_G
Cuts at: 107 107
Size: 5654
NgoMIV G'CCGG_C
Cuts at: 197 3833 197
Size: 3636 2018
NspI r_CATG'y
Cuts at: 1590 1796 2707 3207 4114 1590
Size: 206 911 500 907 3130
Fragments arranged by size:
3130 911 907 500 206
PasI CC'CwG_GG
Cuts at: 2388 2388
Size: 5654
PciI A'CATG_T
Cuts at: 4110 4110
Size: 5654
PflMI CCAn_nnn'nTGG
Cuts at: 750 1075 750
Size: 325 5329
PfoI T'CCnGG_A
Cuts at: 449 2525 449
Size: 2076 3578
PmeI GTTT'AAAC
Cuts at: 2653 2653
Size: 5654
PmlI CAC'GTG
Cuts at: 1518 1518
Size: 5654
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4793 4825 4793
Size: 32 5622
PpuMI rG'GwC_Cy
Cuts at: 552 1191 1254 1807 1853 552
Size: 639 63 553 46 4353
Fragments arranged by size:
4353 639 553 63 46
PspOMI G'GGCC_C
Cuts at: 703 2577 703
Size: 1874 3780
PstI C_TGCA'G
Cuts at: 860 1229 1493 1722 2093 2593 2701 3386 860
Size: 369 264 229 371 500 108 685 3128
Fragments arranged by size:
3128 685 500 371 369 264 229 108
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5654
PvuII CAG'CTG
Cuts at: 512 1419 2458 3079 3439 512
Size: 907 1039 621 360 2727
Fragments arranged by size:
2727 1039 907 621 360
RsrII CG'GwC_CG
Cuts at: 3849 3849
Size: 5654
SacI G_AGCT'C
Cuts at: 720 2668 720
Size: 1948 3706
SacII CC_GC'GG
Cuts at: 956 1273 1533 1951 2313 956
Size: 317 260 418 362 4297
Fragments arranged by size:
4297 418 362 317 260
SalI G'TCGA_C
Cuts at: 2691 2691
Size: 5654
SanDI GG'GwC_CC
Cuts at: 1807 1807
Size: 5654
SapI GCTCTTCn'nnn_
Cuts at: 115 613 3677 3887 115
Size: 498 3064 210 1882
Fragments arranged by size:
3064 1882 498 210
SbfI CC_TGCA'GG
Cuts at: 2701 2701
Size: 5654
ScaI AGT'ACT
Cuts at: 5171 5171
Size: 5654
SfcI C'TryA_G
Cuts at: 33 856 1225 1489 1718 2089 2589 2697 3382
Size: 823 369 264 229 371 500 108 685
Cuts at: 3382 4375 4566 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 823 685 500 371 369 264
229 191 108
SfoI GGC'GCC
Cuts at: 3086 3334 3086
Size: 248 5406
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5654
SmaI CCC'GGG
Cuts at: 325 2676 325
Size: 2351 3303
SmlI C'TyrA_G
Cuts at: 133 643 832 1770 2206 2809 3724 4216 4478
Size: 510 189 938 436 603 915 492 262
Cuts at: 4478 133
Size: 1309
Fragments arranged by size:
1309 938 915 603 510 492 436 262
189
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5654
SphI G_CATG'C
Cuts at: 1590 1796 2707 3207 1590
Size: 206 911 500 4037
Fragments arranged by size:
4037 911 500 206
StuI AGG'CCT
Cuts at: 994 1624 1983 994
Size: 630 359 4665
Fragments arranged by size:
4665 630 359
StyI C'CwwG_G
Cuts at: 107 1343 2076 2788 3142 107
Size: 1236 733 712 354 2619
Fragments arranged by size:
2619 1236 733 712 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 361 2185 2602 2619 3237 3370 3829 4038 361
Size: 1824 417 17 618 133 459 209 1977
Fragments arranged by size:
1977 1824 618 459 417 209 133 17
TatI w'GTAC_w
Cuts at: 500 5169 500
Size: 4669 985
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 147 179 1118 1150 5171 5203 147
Size: 32 939 32 4021 32 598
Fragments arranged by size:
4021 939 598 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3451 3451
Size: 5654
XbaI T'CTAG_A
Cuts at: 2685 2685
Size: 5654
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 675 1733 1878 3149 675
Size: 1058 145 1271 3180
Fragments arranged by size:
3180 1271 1058 145
XmaI C'CCGG_G
Cuts at: 323 2674 323
Size: 2351 3303
ZraI GAC'GTC
Cuts at: 1020 1759 1020
Size: 739 4915
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AloI AscI AvrII BfrBI
BmtI BsiWI BsmI BstZ17I ClaI CspCI DraIII EcoRV
FspAI HindIII MfeI NdeI NheI NotI NruI NsiI
PacI PshAI PsiI PspXI PsrI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI