Restriction map for
pF1KB8476
(Circular) (Six-base) MAPSORT of: pF1KB8476.seq Check: 1007 from: 1 to: 5651
pF1KB8476 5651 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AatII G_ACGT'C
Cuts at: 209 209
Size: 5651
AccI GT'mk_AC
Cuts at: 2689 2689
Size: 5651
Acc65I G'GTAC_C
Cuts at: 2667 2667
Size: 5651
AclI AA'CG_TT
Cuts at: 5491 5491
Size: 5651
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 1040 1094 1334 1715 2038 3476 3908 4655
Size: 307 54 240 381 323 1438 432 747
Cuts at: 4655 4923 733
Size: 268 1461
Fragments arranged by size:
1461 1438 747 432 381 323 307 268
240 54
AflIII A'CryG_T
Cuts at: 3003 4107 5084 3003
Size: 1104 977 3570
Fragments arranged by size:
3570 1104 977
AgeI A'CCGG_T
Cuts at: 4039 4039
Size: 5651
AleI CACnn'nnGTG
Cuts at: 1764 1764
Size: 5651
AlwNI CAG_nnn'CTG
Cuts at: 2956 4523 2956
Size: 1567 4084
ApaLI G'TGCA_C
Cuts at: 4421 4421
Size: 5651
ApoI r'AATT_y
Cuts at: 1119 1200 1388 1933 2077 2341 2655 5004 5260
Size: 81 188 545 144 264 314 2349 256
Cuts at: 5260 1119
Size: 1510
Fragments arranged by size:
2349 1510 545 314 264 256 188 144
81
AseI AT'TA_AT
Cuts at: 20 1634 20
Size: 1614 4037
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5651
AvaI C'yCGr_G
Cuts at: 2671 2671
Size: 5651
BamHI G'GATC_C
Cuts at: 2676 2676
Size: 5651
BanI G'GyrC_C
Cuts at: 193 637 2667 3081 3329 3364 193
Size: 444 2030 414 248 35 2480
Fragments arranged by size:
2480 2030 444 414 248 35
BanII G_rGCy'C
Cuts at: 786 963 1046 1383 2665 3695 786
Size: 177 83 337 1282 1030 2742
Fragments arranged by size:
2742 1282 1030 337 177 83
BbeI G_GCGC'C
Cuts at: 3085 3333 3085
Size: 248 5403
BbsI GAAGACnn'nnnn_
Cuts at: 1322 1404 1322
Size: 82 5569
BciVI GTATCCnnnnn_n'
Cuts at: 1646 3549 4310 5651 1646
Size: 1903 761 1341 1646
Fragments arranged by size:
1903 1646 1341 761
BclI T'GATC_A
Cuts at: 3171 3171
Size: 5651
BfrBI ATG'CAT
Cuts at: 519 519
Size: 5651
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5651
BglII A'GATC_T
Cuts at: 1072 3166 5203 1072
Size: 2094 2037 1520
Fragments arranged by size:
2094 2037 1520
BlpI GC'TnA_GC
Cuts at: 2763 2763
Size: 5651
Bme1580I G_kGCm'C
Cuts at: 3276 3369 4425 3276
Size: 93 1056 4502
Fragments arranged by size:
4502 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2863 2944 3279 5423 2863
Size: 81 335 2144 3091
Fragments arranged by size:
3091 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4018 4018
Size: 5651
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 293
Size: 5651
Bpu10I CC'TnA_GC
Cuts at: 1289 1289
Size: 5651
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2827 3706 4198 4496 2827
Size: 879 492 298 3982
Fragments arranged by size:
3982 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 955 5355 955
Size: 4400 1251
BsaAI yAC'GTr
Cuts at: 3004 3004
Size: 5651
BsaBI GATnn'nnATC
Cuts at: 6 1188 3165 5202 6
Size: 1182 1977 2037 455
Fragments arranged by size:
2037 1977 1182 455
BsaHI Gr'CG_yC
Cuts at: 206 3082 3330 4032 5528 206
Size: 2876 248 702 1496 329
Fragments arranged by size:
2876 1496 702 329 248
BsaWI w'CCGG_w
Cuts at: 1326 2840 3361 4039 4313 4460 4871 1326
Size: 1514 521 678 274 147 411 2106
Fragments arranged by size:
2106 1514 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1136 1136
Size: 5651
BseYI C'CCAG_C
Cuts at: 3076 3834 4411 4907 3076
Size: 758 577 496 3820
Fragments arranged by size:
3820 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2288 2585 2288
Size: 297 5354
BsiEI CG_ry'CG
Cuts at: 104 3193 3239 4447 104
Size: 3089 46 1208 1308
Fragments arranged by size:
3089 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1383 2665 3443 3633 4425 1383
Size: 1282 778 190 792 2609
Fragments arranged by size:
2609 1282 792 778 190
BsmI GAATG_Cn'
Cuts at: 1092 1092
Size: 5651
Bsp1286I G_dGCh'C
Cuts at: 786 963 1046 1383 2665 3276 3369 3443 3633
Size: 177 83 337 1282 611 93 74 190
Cuts at: 3633 3695 4425 786
Size: 62 730 2012
Fragments arranged by size:
2012 1282 730 611 337 190 177 93
83 74 62
BspEI T'CCGG_A
Cuts at: 1326 4871 1326
Size: 3545 2106
BspHI T'CATG_A
Cuts at: 4827 4827
Size: 5651
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2701 2915 3217 3598 2701
Size: 214 302 381 4754
Fragments arranged by size:
4754 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3943 3997 5481 5646 50
Size: 3893 54 1484 165 55
Fragments arranged by size:
3893 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 611 3563 611
Size: 2952 2699
BsrFI r'CCGG_y
Cuts at: 3649 3830 4039 3649
Size: 181 209 5261
Fragments arranged by size:
5261 209 181
BsrGI T'GTAC_A
Cuts at: 225 225
Size: 5651
BssSI C'ACGA_G
Cuts at: 1169 3922 4280 1169
Size: 2753 358 2540
Fragments arranged by size:
2753 2540 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3028 3028
Size: 5651
BstBI TT'CG_AA
Cuts at: 4012 4012
Size: 5651
BstEII G'GTnAC_C
Cuts at: 2505 2505
Size: 5651
BstXI CCAn_nnnn'nTGG
Cuts at: 433 433
Size: 5651
BstYI r'GATC_y
Cuts at: 1 1072 2676 3143 3166 3501 3747 4759 4845
Size: 1071 1604 467 23 335 246 1012 86
Cuts at: 4845 4857 5203 1
Size: 12 346 449
Fragments arranged by size:
1604 1071 1012 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 142 142
Size: 5651
BtgI C'CryG_G
Cuts at: 107 131 558 1759 107
Size: 24 427 1201 3999
Fragments arranged by size:
3999 1201 427 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 578 3601 3782 3945 578
Size: 3023 181 163 2284
Fragments arranged by size:
3023 2284 181 163
BtsI GCAGTG_nn'
Cuts at: 2894 2894
Size: 5651
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1963 1998 1963
Size: 35 5616
DraI TTT'AAA
Cuts at: 2650 2650
Size: 5651
DrdI GACnn_nn'nnGTC
Cuts at: 2563 3357 4215 2563
Size: 794 858 3999
Fragments arranged by size:
3999 858 794
EaeI y'GGCC_r
Cuts at: 3236 3410 3801 3828 5173 3236
Size: 174 391 27 1345 3714
Fragments arranged by size:
3714 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3236 3236
Size: 5651
EarI CTCTTCn'nnn_
Cuts at: 793 3674 3884 793
Size: 2881 210 2560
Fragments arranged by size:
2881 2560 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 162 419 3859 4169 4315 5461 162
Size: 257 3440 310 146 1146 352
Fragments arranged by size:
3440 1146 352 310 257 146
EcoICRI GAG'CTC
Cuts at: 1381 2663 1381
Size: 1282 4369
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 733 1040 1094 1334 1715 2038 3476 3908
Size: 440 307 54 240 381 323 1438 432
Cuts at: 3908 4655 4923 293
Size: 747 268 1021
Fragments arranged by size:
1438 1021 747 440 432 381 323 307
268 240 54
EcoO109I rG'GnC_Cy
Cuts at: 114 2790 114
Size: 2676 2975
EcoRI G'AATT_C
Cuts at: 2655 2655
Size: 5651
EcoRV GAT'ATC
Cuts at: 528 708 528
Size: 180 5471
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3670 3670
Size: 5651
FspI TGC'GCA
Cuts at: 3432 3432
Size: 5651
HaeII r_GCGC'y
Cuts at: 3085 3333 4355 5334 3085
Size: 248 1022 979 3402
Fragments arranged by size:
3402 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3493 3523 3525 3555 3493
Size: 30 2 30 5589
Fragments arranged by size:
5589 30 30 2
HincII GTy'rAC
Cuts at: 2690 2690
Size: 5651
HindIII A'AGCT_T
Cuts at: 2487 2487
Size: 5651
KasI G'GCGC_C
Cuts at: 3081 3329 3081
Size: 248 5403
KpnI G_GTAC'C
Cuts at: 2671 2671
Size: 5651
MfeI C'AATT_G
Cuts at: 731 731
Size: 5651
MluI A'CGCG_T
Cuts at: 5084 5084
Size: 5651
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2288 4322 4506 2288
Size: 2034 184 3433
Fragments arranged by size:
3433 2034 184
MscI TGG'CCA
Cuts at: 3412 3412
Size: 5651
MslI CAynn'nnrTG
Cuts at: 563 1764 2247 3640 3767 563
Size: 1201 483 1393 127 2447
Fragments arranged by size:
2447 1393 1201 483 127
MspA1I CmG'CkG
Cuts at: 120 133 191 2762 3076 3436 4449 4694 120
Size: 13 58 2571 314 360 1013 245 1077
Fragments arranged by size:
2571 1077 1013 360 314 245 58 13
NaeI GCC'GGC
Cuts at: 3832 3832
Size: 5651
NarI GG'CG_CC
Cuts at: 3082 3330 3082
Size: 248 5403
NcoI C'CATG_G
Cuts at: 107 558 107
Size: 451 5200
NgoMIV G'CCGG_C
Cuts at: 3830 3830
Size: 5651
NsiI A_TGCA'T
Cuts at: 521 521
Size: 5651
NspI r_CATG'y
Cuts at: 1598 1867 2704 3204 4111 1598
Size: 269 837 500 907 3138
Fragments arranged by size:
3138 907 837 500 269
PciI A'CATG_T
Cuts at: 4107 4107
Size: 5651
PflMI CCAn_nnn'nTGG
Cuts at: 1417 1417
Size: 5651
PfoI T'CCnGG_A
Cuts at: 1648 1648
Size: 5651
PmeI GTTT'AAAC
Cuts at: 2650 2650
Size: 5651
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4790 4822 4790
Size: 32 5619
PstI C_TGCA'G
Cuts at: 249 2698 3383 249
Size: 2449 685 2517
Fragments arranged by size:
2517 2449 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5651
PvuII CAG'CTG
Cuts at: 3076 3436 3076
Size: 360 5291
RsrII CG'GwC_CG
Cuts at: 3846 3846
Size: 5651
SacI G_AGCT'C
Cuts at: 1383 2665 1383
Size: 1282 4369
SacII CC_GC'GG
Cuts at: 134 134
Size: 5651
SalI G'TCGA_C
Cuts at: 2688 2688
Size: 5651
SapI GCTCTTCn'nnn_
Cuts at: 3674 3884 3674
Size: 210 5441
SbfI CC_TGCA'GG
Cuts at: 2698 2698
Size: 5651
ScaI AGT'ACT
Cuts at: 5168 5168
Size: 5651
SfcI C'TryA_G
Cuts at: 33 245 2429 2694 3379 4372 4563 33
Size: 212 2184 265 685 993 191 1121
Fragments arranged by size:
2184 1121 993 685 265 212 191
SfoI GGC'GCC
Cuts at: 3083 3331 3083
Size: 248 5403
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5651
SmaI CCC'GGG
Cuts at: 2673 2673
Size: 5651
SmlI C'TyrA_G
Cuts at: 2806 3721 4213 4475 2806
Size: 915 492 262 3982
Fragments arranged by size:
3982 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5651
SphI G_CATG'C
Cuts at: 1598 2704 3204 1598
Size: 1106 500 4045
Fragments arranged by size:
4045 1106 500
SspI AAT'ATT
Cuts at: 305 543 305
Size: 238 5413
StyI C'CwwG_G
Cuts at: 107 558 1294 2785 3139 107
Size: 451 736 1491 354 2619
Fragments arranged by size:
2619 1491 736 451 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 788 1584 1931 3234 3367 3826 4035 788
Size: 796 347 1303 133 459 209 2404
Fragments arranged by size:
2404 1303 796 459 347 209 133
TatI w'GTAC_w
Cuts at: 225 1676 1768 5166 225
Size: 1451 92 3398 710
Fragments arranged by size:
3398 1451 710 92
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5168 5200 5168
Size: 32 5619
Tth111I GACn'n_nGTC
Cuts at: 3448 3448
Size: 5651
XbaI T'CTAG_A
Cuts at: 955 2682 955
Size: 1727 3924
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3146 3146
Size: 5651
XmaI C'CCGG_G
Cuts at: 2671 2671
Size: 5651
XmnI GAAnn'nnTTC
Cuts at: 917 917
Size: 5651
ZraI GAC'GTC
Cuts at: 207 207
Size: 5651
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BmgBI BmtI BsaXI BsiWI
BsmBI BssHII BstZ17I ClaI DraIII EcoNI FseI FspAI
HpaI NdeI NheI NotI NruI PacI PasI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI