Restriction map for
pF1KB8475
(Circular) (Six-base) MAPSORT of: pF1KB8475.seq Check: 2983 from: 1 to: 5762
pF1KB8475 5762 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AatII G_ACGT'C
Cuts at: 1158 1158
Size: 5762
AccI GT'mk_AC
Cuts at: 2379 2800 2379
Size: 421 5341
Acc65I G'GTAC_C
Cuts at: 2778 2778
Size: 5762
AclI AA'CG_TT
Cuts at: 5602 5602
Size: 5762
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1927 3587 4019 4766 5034 1927
Size: 1660 432 747 268 2655
Fragments arranged by size:
2655 1660 747 432 268
AflII C'TTAA_G
Cuts at: 1843 2194 1843
Size: 351 5411
AflIII A'CryG_T
Cuts at: 1199 2382 3114 4218 5195 1199
Size: 1183 732 1104 977 1766
Fragments arranged by size:
1766 1183 1104 977 732
AgeI A'CCGG_T
Cuts at: 4150 4150
Size: 5762
AleI CACnn'nnGTG
Cuts at: 620 2069 620
Size: 1449 4313
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1778 1810 1778
Size: 32 5730
AlwNI CAG_nnn'CTG
Cuts at: 2413 3067 4634 2413
Size: 654 1567 3541
Fragments arranged by size:
3541 1567 654
ApaLI G'TGCA_C
Cuts at: 4532 4532
Size: 5762
ApoI r'AATT_y
Cuts at: 358 382 691 808 1964 2766 5115 5371 358
Size: 24 309 117 1156 802 2349 256 749
Fragments arranged by size:
2349 1156 802 749 309 256 117 24
AseI AT'TA_AT
Cuts at: 20 1928 20
Size: 1908 3854
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5762
AvaI C'yCGr_G
Cuts at: 1049 2782 1049
Size: 1733 4029
BamHI G'GATC_C
Cuts at: 2787 2787
Size: 5762
BanI G'GyrC_C
Cuts at: 2778 3192 3440 3475 2778
Size: 414 248 35 5065
Fragments arranged by size:
5065 414 248 35
BanII G_rGCy'C
Cuts at: 1100 2565 2577 2776 3806 1100
Size: 1465 12 199 1030 3056
Fragments arranged by size:
3056 1465 1030 199 12
BbeI G_GCGC'C
Cuts at: 3196 3444 3196
Size: 248 5514
BbsI GAAGACnn'nnnn_
Cuts at: 1322 1580 1322
Size: 258 5504
BciVI GTATCCnnnnn_n'
Cuts at: 2683 3660 4421 5762 2683
Size: 977 761 1341 2683
Fragments arranged by size:
2683 1341 977 761
BclI T'GATC_A
Cuts at: 3282 3282
Size: 5762
BglII A'GATC_T
Cuts at: 972 2737 3277 5314 972
Size: 1765 540 2037 1420
Fragments arranged by size:
2037 1765 1420 540
BlpI GC'TnA_GC
Cuts at: 1178 1235 2874 1178
Size: 57 1639 4066
Fragments arranged by size:
4066 1639 57
Bme1580I G_kGCm'C
Cuts at: 3387 3480 4536 3387
Size: 93 1056 4613
Fragments arranged by size:
4613 1056 93
BmgBI CAC'GTC
Cuts at: 1200 1200
Size: 5762
BmrI ACTGGGnnnn_n'
Cuts at: 149 1811 2974 3055 3390 5534 149
Size: 1662 1163 81 335 2144 377
Fragments arranged by size:
2144 1662 1163 377 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4129 4129
Size: 5762
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1076 1331 1076
Size: 255 5507
Bpu10I CC'TnA_GC
Cuts at: 1094 1094
Size: 5762
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1084 1723 2938 3817 4309 4607 1084
Size: 639 1215 879 492 298 2239
Fragments arranged by size:
2239 1215 879 639 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5466 5466
Size: 5762
BsaAI yAC'GTr
Cuts at: 3115 3115
Size: 5762
BsaBI GATnn'nnATC
Cuts at: 6 1865 3276 5313 6
Size: 1859 1411 2037 455
Fragments arranged by size:
2037 1859 1411 455
BsaHI Gr'CG_yC
Cuts at: 1155 3193 3441 4143 5639 1155
Size: 2038 248 702 1496 1278
Fragments arranged by size:
2038 1496 1278 702 248
BsaWI w'CCGG_w
Cuts at: 378 2951 3472 4150 4424 4571 4982 378
Size: 2573 521 678 274 147 411 1158
Fragments arranged by size:
2573 1158 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1641 1671 2072 2102 1641
Size: 30 401 30 5301
Fragments arranged by size:
5301 401 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1516 2093 1516
Size: 577 5185
BseYI C'CCAG_C
Cuts at: 1998 2184 3187 3945 4522 5018 1998
Size: 186 1003 758 577 496 2742
Fragments arranged by size:
2742 1003 758 577 496 186
BsiEI CG_ry'CG
Cuts at: 104 3304 3350 4558 104
Size: 3200 46 1208 1308
Fragments arranged by size:
3200 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1100 2066 2565 2776 3554 3744 4536 1100
Size: 966 499 211 778 190 792 2326
Fragments arranged by size:
2326 966 792 778 499 211 190
BsmI GAATG_Cn'
Cuts at: 1034 1239 2533 1034
Size: 205 1294 4263
Fragments arranged by size:
4263 1294 205
Bsp1286I G_dGCh'C
Cuts at: 1100 2066 2565 2577 2776 3387 3480 3554 3744
Size: 966 499 12 199 611 93 74 190
Cuts at: 3744 3806 4536 1100
Size: 62 730 2326
Fragments arranged by size:
2326 966 730 611 499 199 190 93
74 62 12
BspEI T'CCGG_A
Cuts at: 378 4982 378
Size: 4604 1158
BspHI T'CATG_A
Cuts at: 4938 4938
Size: 5762
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2659 2812 3026 3328 3709 2659
Size: 153 214 302 381 4712
Fragments arranged by size:
4712 381 302 214 153
BsrBI CCG'CTC
Cuts at: 50 4054 4108 5592 5757 50
Size: 4004 54 1484 165 55
Fragments arranged by size:
4004 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2628 3674 2628
Size: 1046 4716
BsrFI r'CCGG_y
Cuts at: 3760 3941 4150 3760
Size: 181 209 5372
Fragments arranged by size:
5372 209 181
BsrGI T'GTAC_A
Cuts at: 532 676 1545 532
Size: 144 869 4749
Fragments arranged by size:
4749 869 144
BssSI C'ACGA_G
Cuts at: 4033 4391 4033
Size: 358 5404
BstAPI GCAn_nnn'nTGC
Cuts at: 3139 3139
Size: 5762
BstBI TT'CG_AA
Cuts at: 633 2568 4123 633
Size: 1935 1555 2272
Fragments arranged by size:
2272 1935 1555
BstEII G'GTnAC_C
Cuts at: 1811 1811
Size: 5762
BstYI r'GATC_y
Cuts at: 1 217 972 2737 2787 3254 3277 3612 3858
Size: 216 755 1765 50 467 23 335 246
Cuts at: 3858 4870 4956 4968 5314 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1765 1012 755 467 449 346 335 246
216 86 50 23 12
BtgI C'CryG_G
Cuts at: 107 2589 107
Size: 2482 3280
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3712 3893 4056 3712
Size: 181 163 5418
Fragments arranged by size:
5418 181 163
BtsI GCAGTG_nn'
Cuts at: 3005 3005
Size: 5762
DraI TTT'AAA
Cuts at: 813 1163 1835 2761 813
Size: 350 672 926 3814
Fragments arranged by size:
3814 926 672 350
DrdI GACnn_nn'nnGTC
Cuts at: 1200 3468 4326 1200
Size: 2268 858 2636
Fragments arranged by size:
2636 2268 858
EaeI y'GGCC_r
Cuts at: 2169 3347 3521 3912 3939 5284 2169
Size: 1178 174 391 27 1345 2647
Fragments arranged by size:
2647 1345 1178 391 174 27
EagI C'GGCC_G
Cuts at: 3347 3347
Size: 5762
EarI CTCTTCn'nnn_
Cuts at: 168 233 860 950 1457 2101 2107 2570 3785
Size: 65 627 90 507 644 6 463 1215
Cuts at: 3785 3995 168
Size: 210 1935
Fragments arranged by size:
1935 1215 644 627 507 463 210 90
65 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 777 3970 4280 4426 5572 777
Size: 3193 310 146 1146 967
Fragments arranged by size:
3193 1146 967 310 146
EcoICRI GAG'CTC
Cuts at: 1098 2563 2774 1098
Size: 1465 211 4086
Fragments arranged by size:
4086 1465 211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1076 1331 1927 3587 4019 4766 5034 1076
Size: 255 596 1660 432 747 268 1804
Fragments arranged by size:
1804 1660 747 596 432 268 255
EcoNI CCTnn'n_nnAGG
Cuts at: 439 439
Size: 5762
EcoO109I rG'GnC_Cy
Cuts at: 2901 2901
Size: 5762
EcoRI G'AATT_C
Cuts at: 2766 2766
Size: 5762
EcoRV GAT'ATC
Cuts at: 1269 1536 1269
Size: 267 5495
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 222 316 1189 2630 3781 222
Size: 94 873 1441 1151 2203
Fragments arranged by size:
2203 1441 1151 873 94
FspI TGC'GCA
Cuts at: 3543 3543
Size: 5762
HaeII r_GCGC'y
Cuts at: 1227 3196 3444 4466 5445 1227
Size: 1969 248 1022 979 1544
Fragments arranged by size:
1969 1544 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2361 2393 2428 2460 3604 3634 3636 3666 2361
Size: 32 35 32 1144 30 2 30 4457
Fragments arranged by size:
4457 1144 35 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2380 2801 2380
Size: 421 5341
HindIII A'AGCT_T
Cuts at: 749 2025 749
Size: 1276 4486
KasI G'GCGC_C
Cuts at: 3192 3440 3192
Size: 248 5514
KpnI G_GTAC'C
Cuts at: 2782 2782
Size: 5762
MluI A'CGCG_T
Cuts at: 5195 5195
Size: 5762
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1298 1821 4433 4617 1298
Size: 523 2612 184 2443
Fragments arranged by size:
2612 2443 523 184
MscI TGG'CCA
Cuts at: 2171 3523 2171
Size: 1352 4410
MslI CAynn'nnrTG
Cuts at: 325 513 620 1039 1928 2069 3751 3878 325
Size: 188 107 419 889 141 1682 127 2209
Fragments arranged by size:
2209 1682 889 419 188 141 127 107
MspA1I CmG'CkG
Cuts at: 1234 1858 2037 2873 3187 3547 4560 4805 1234
Size: 624 179 836 314 360 1013 245 2191
Fragments arranged by size:
2191 1013 836 624 360 314 245 179
NaeI GCC'GGC
Cuts at: 3943 3943
Size: 5762
NarI GG'CG_CC
Cuts at: 3193 3441 3193
Size: 248 5514
NcoI C'CATG_G
Cuts at: 107 107
Size: 5762
NdeI CA'TA_TG
Cuts at: 228 228
Size: 5762
NgoMIV G'CCGG_C
Cuts at: 3941 3941
Size: 5762
NspI r_CATG'y
Cuts at: 2386 2635 2815 3315 4222 2386
Size: 249 180 500 907 3926
Fragments arranged by size:
3926 907 500 249 180
PciI A'CATG_T
Cuts at: 2382 4218 2382
Size: 1836 3926
PflMI CCAn_nnn'nTGG
Cuts at: 618 1765 618
Size: 1147 4615
PmeI GTTT'AAAC
Cuts at: 2761 2761
Size: 5762
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4901 4933 4901
Size: 32 5730
PshAI GACnn'nnGTC
Cuts at: 607 607
Size: 5762
PsiI TTA'TAA
Cuts at: 304 746 304
Size: 442 5320
PstI C_TGCA'G
Cuts at: 2809 3494 2809
Size: 685 5077
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5762
PvuII CAG'CTG
Cuts at: 1234 1858 3187 3547 1234
Size: 624 1329 360 3449
Fragments arranged by size:
3449 1329 624 360
RsrII CG'GwC_CG
Cuts at: 3957 3957
Size: 5762
SacI G_AGCT'C
Cuts at: 1100 2565 2776 1100
Size: 1465 211 4086
Fragments arranged by size:
4086 1465 211
SalI G'TCGA_C
Cuts at: 2378 2799 2378
Size: 421 5341
SapI GCTCTTCn'nnn_
Cuts at: 2570 3785 3995 2570
Size: 1215 210 4337
Fragments arranged by size:
4337 1215 210
SbfI CC_TGCA'GG
Cuts at: 2809 2809
Size: 5762
ScaI AGT'ACT
Cuts at: 554 5279 554
Size: 4725 1037
SfcI C'TryA_G
Cuts at: 33 2805 3490 4483 4674 33
Size: 2772 685 993 191 1121
Fragments arranged by size:
2772 1121 993 685 191
SfoI GGC'GCC
Cuts at: 3194 3442 3194
Size: 248 5514
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5762
SmaI CCC'GGG
Cuts at: 2784 2784
Size: 5762
SmlI C'TyrA_G
Cuts at: 1099 1738 1843 2194 2917 3832 4324 4586 1099
Size: 639 105 351 723 915 492 262 2275
Fragments arranged by size:
2275 915 723 639 492 351 262 105
SpeI A'CTAG_T
Cuts at: 67 588 67
Size: 521 5241
SphI G_CATG'C
Cuts at: 2815 3315 2815
Size: 500 5262
SspI AAT'ATT
Cuts at: 913 1630 913
Size: 717 5045
StyI C'CwwG_G
Cuts at: 107 197 2896 3250 107
Size: 90 2699 354 2619
Fragments arranged by size:
2699 2619 354 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 610 3345 3478 3937 4146 610
Size: 2735 133 459 209 2226
Fragments arranged by size:
2735 2226 459 209 133
TatI w'GTAC_w
Cuts at: 532 552 676 1321 1545 1803 2031 2476 5277
Size: 20 124 645 224 258 228 445 2801
Cuts at: 5277 532
Size: 1017
Fragments arranged by size:
2801 1017 645 445 258 228 224 124
20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1335 1367 5279 5311 1335
Size: 32 3912 32 1786
Fragments arranged by size:
3912 1786 32 32
Tth111I GACn'n_nGTC
Cuts at: 3559 3559
Size: 5762
XbaI T'CTAG_A
Cuts at: 2793 2793
Size: 5762
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 619 2357 3257 619
Size: 1738 900 3124
Fragments arranged by size:
3124 1738 900
XmaI C'CCGG_G
Cuts at: 2782 2782
Size: 5762
XmnI GAAnn'nnTTC
Cuts at: 2613 2613
Size: 5762
ZraI GAC'GTC
Cuts at: 1156 1156
Size: 5762
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AlfI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmtI BsgI BsiWI BsmBI
BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII FseI
FspAI HpaI MfeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI