Restriction map for
pF1KB8470
(Circular) (Six-base) MAPSORT of: pF1KB8470.seq Check: 9732 from: 1 to: 5579
pF1KB8470 5579 bp
With 182 enzymes: SgfI *
July 28, 2010 10:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 760 760
Size: 5579
AatII G_ACGT'C
Cuts at: 1373 2315 1373
Size: 942 4637
AccI GT'mk_AC
Cuts at: 2102 2555 2617 2102
Size: 453 62 5064
Fragments arranged by size:
5064 453 62
Acc65I G'GTAC_C
Cuts at: 2369 2595 2369
Size: 226 5353
AclI AA'CG_TT
Cuts at: 5419 5419
Size: 5579
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1036 1849 2512 3404 3836 4583 4851 1036
Size: 813 663 892 432 747 268 1764
Fragments arranged by size:
1764 892 813 747 663 432 268
AfeI AGC'GCT
Cuts at: 1049 1049
Size: 5579
AflII C'TTAA_G
Cuts at: 855 1468 855
Size: 613 4966
AflIII A'CryG_T
Cuts at: 1110 2207 2931 4035 5012 1110
Size: 1097 724 1104 977 1677
Fragments arranged by size:
1677 1104 1097 977 724
AgeI A'CCGG_T
Cuts at: 1384 3967 1384
Size: 2583 2996
AlwNI CAG_nnn'CTG
Cuts at: 1606 2067 2150 2884 4451 1606
Size: 461 83 734 1567 2734
Fragments arranged by size:
2734 1567 734 461 83
ApaLI G'TGCA_C
Cuts at: 2125 4349 2125
Size: 2224 3355
ApoI r'AATT_y
Cuts at: 317 418 2293 2583 4932 5188 317
Size: 101 1875 290 2349 256 708
Fragments arranged by size:
2349 1875 708 290 256 101
AseI AT'TA_AT
Cuts at: 20 20
Size: 5579
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5579
AvaI C'yCGr_G
Cuts at: 2201 2599 2201
Size: 398 5181
BamHI G'GATC_C
Cuts at: 2604 2604
Size: 5579
BanI G'GyrC_C
Cuts at: 2369 2595 3009 3257 3292 2369
Size: 226 414 248 35 4656
Fragments arranged by size:
4656 414 248 226 35
BanII G_rGCy'C
Cuts at: 853 1880 2489 2593 3623 853
Size: 1027 609 104 1030 2809
Fragments arranged by size:
2809 1030 1027 609 104
BbeI G_GCGC'C
Cuts at: 3013 3261 3013
Size: 248 5331
BbsI GAAGACnn'nnnn_
Cuts at: 1828 1828
Size: 5579
BbvCI CC'TCA_GC
Cuts at: 2027 2027
Size: 5579
BciVI GTATCCnnnnn_n'
Cuts at: 3477 4238 5579 3477
Size: 761 1341 3477
Fragments arranged by size:
3477 1341 761
BclI T'GATC_A
Cuts at: 3099 3099
Size: 5579
BglII A'GATC_T
Cuts at: 896 1805 3094 5131 896
Size: 909 1289 2037 1344
Fragments arranged by size:
2037 1344 1289 909
BlpI GC'TnA_GC
Cuts at: 2691 2691
Size: 5579
Bme1580I G_kGCm'C
Cuts at: 2001 2129 2443 3204 3297 4353 2001
Size: 128 314 761 93 1056 3227
Fragments arranged by size:
3227 1056 761 314 128 93
BmgBI CAC'GTC
Cuts at: 209 2208 209
Size: 1999 3580
BmrI ACTGGGnnnn_n'
Cuts at: 1644 2791 2872 3207 5351 1644
Size: 1147 81 335 2144 1872
Fragments arranged by size:
2144 1872 1147 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1592 3946 1592
Size: 2354 3225
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 1129 1876 2255 2337 204
Size: 925 747 379 82 3446
Fragments arranged by size:
3446 925 747 379 82
Bpu10I CC'TnA_GC
Cuts at: 1793 2027 1793
Size: 234 5345
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1663 2755 3634 4126 4424 1663
Size: 1092 879 492 298 2818
Fragments arranged by size:
2818 1092 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5283 5283
Size: 5579
BsaAI yAC'GTr
Cuts at: 2438 2932 2438
Size: 494 5085
BsaBI GATnn'nnATC
Cuts at: 6 3093 5130 6
Size: 3087 2037 455
Fragments arranged by size:
3087 2037 455
BsaHI Gr'CG_yC
Cuts at: 1370 1922 2110 2312 3010 3258 3960 5456 1370
Size: 552 188 202 698 248 702 1496 1493
Fragments arranged by size:
1496 1493 702 698 552 248 202 188
BsaWI w'CCGG_w
Cuts at: 777 1384 2550 2768 3289 3967 4241 4388 4799
Size: 607 1166 218 521 678 274 147 411
Cuts at: 4799 777
Size: 1557
Fragments arranged by size:
1557 1166 678 607 521 411 274 218
147
BseYI C'CCAG_C
Cuts at: 1506 2078 2114 2466 3004 3762 4339 4835 1506
Size: 572 36 352 538 758 577 496 2250
Fragments arranged by size:
2250 758 577 572 538 496 352 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 261 261
Size: 5579
BsiEI CG_ry'CG
Cuts at: 104 1389 3121 3167 4375 104
Size: 1285 1732 46 1208 1308
Fragments arranged by size:
1732 1308 1285 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1880 1904 2129 2593 3371 3561 4353 1880
Size: 24 225 464 778 190 792 3106
Fragments arranged by size:
3106 792 778 464 225 190 24
BsmI GAATG_Cn'
Cuts at: 1013 1780 1013
Size: 767 4812
Bsp1286I G_dGCh'C
Cuts at: 853 1880 1904 2001 2129 2443 2489 2593 3204
Size: 1027 24 97 128 314 46 104 611
Cuts at: 3204 3297 3371 3561 3623 4353 853
Size: 93 74 190 62 730 2079
Fragments arranged by size:
2079 1027 730 611 314 190 128 104
97 93 74 62 46 24
BspEI T'CCGG_A
Cuts at: 4799 4799
Size: 5579
BspHI T'CATG_A
Cuts at: 4755 4755
Size: 5579
BspMI ACCTGCnnnn'nnnn_
Cuts at: 464 680 760 2629 2843 3145 3526 464
Size: 216 80 1869 214 302 381 2517
Fragments arranged by size:
2517 1869 381 302 216 214 80
BsrBI CCG'CTC
Cuts at: 50 3871 3925 5409 5574 50
Size: 3821 54 1484 165 55
Fragments arranged by size:
3821 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1530 3491 1530
Size: 1961 3618
BsrFI r'CCGG_y
Cuts at: 1384 3577 3758 3967 1384
Size: 2193 181 209 2996
Fragments arranged by size:
2996 2193 209 181
BsrGI T'GTAC_A
Cuts at: 2210 2285 2210
Size: 75 5504
BssSI C'ACGA_G
Cuts at: 3850 4208 3850
Size: 358 5221
BstAPI GCAn_nnn'nTGC
Cuts at: 2067 2124 2956 2067
Size: 57 832 4690
Fragments arranged by size:
4690 832 57
BstBI TT'CG_AA
Cuts at: 3940 3940
Size: 5579
BstEII G'GTnAC_C
Cuts at: 805 805
Size: 5579
BstXI CCAn_nnnn'nTGG
Cuts at: 1657 1657
Size: 5579
BstYI r'GATC_y
Cuts at: 1 233 297 896 1628 1805 2604 3071 3094
Size: 232 64 599 732 177 799 467 23
Cuts at: 3094 3429 3675 4687 4773 4785 5131 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 799 732 599 467 449 346 335
246 232 177 86 64 23 12
Bsu36I CC'TnA_GG
Cuts at: 1993 1993
Size: 5579
BtgI C'CryG_G
Cuts at: 140 1134 2042 2090 140
Size: 994 908 48 3629
Fragments arranged by size:
3629 994 908 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 309 3529 3710 3873 309
Size: 3220 181 163 2015
Fragments arranged by size:
3220 2015 181 163
BtsI GCAGTG_nn'
Cuts at: 228 1823 2822 228
Size: 1595 999 2985
Fragments arranged by size:
2985 1595 999
DraI TTT'AAA
Cuts at: 2578 2578
Size: 5579
DraIII CAC_nnn'GTG
Cuts at: 2124 2124
Size: 5579
DrdI GACnn_nn'nnGTC
Cuts at: 495 3285 4143 495
Size: 2790 858 1931
Fragments arranged by size:
2790 1931 858
EaeI y'GGCC_r
Cuts at: 710 2138 2238 2537 3164 3338 3729 3756 5101
Size: 1428 100 299 627 174 391 27 1345
Cuts at: 5101 710
Size: 1188
Fragments arranged by size:
1428 1345 1188 627 391 299 174 100
27
EagI C'GGCC_G
Cuts at: 3164 3164
Size: 5579
EarI CTCTTCn'nnn_
Cuts at: 3602 3812 3602
Size: 210 5369
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 925 3787 4097 4243 5389 925
Size: 2862 310 146 1146 1115
Fragments arranged by size:
2862 1146 1115 310 146
EcoICRI GAG'CTC
Cuts at: 1878 2591 1878
Size: 713 4866
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 1036 1129 1849 1876 2255 2337 2512 3404
Size: 832 93 720 27 379 82 175 892
Cuts at: 3404 3836 4583 4851 204
Size: 432 747 268 932
Fragments arranged by size:
932 892 832 747 720 432 379 268
175 93 82 27
EcoNI CCTnn'n_nnAGG
Cuts at: 120 120
Size: 5579
EcoO109I rG'GnC_Cy
Cuts at: 1977 2718 1977
Size: 741 4838
EcoRI G'AATT_C
Cuts at: 2583 2583
Size: 5579
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3598 3598
Size: 5579
FspI TGC'GCA
Cuts at: 3360 3360
Size: 5579
HaeII r_GCGC'y
Cuts at: 1051 3013 3261 4283 5262 1051
Size: 1962 248 1022 979 1368
Fragments arranged by size:
1962 1368 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 573 589 605 621 1218 1250 1519 1551 3421
Size: 16 16 16 597 32 269 32 1870
Cuts at: 3421 3451 3453 3483 573
Size: 30 2 30 2669
Fragments arranged by size:
2669 1870 597 269 32 32 30 30
16 16 16 2
HincII GTy'rAC
Cuts at: 945 2618 945
Size: 1673 3906
HindIII A'AGCT_T
Cuts at: 371 371
Size: 5579
KasI G'GCGC_C
Cuts at: 3009 3257 3009
Size: 248 5331
KpnI G_GTAC'C
Cuts at: 2373 2599 2373
Size: 226 5353
MfeI C'AATT_G
Cuts at: 398 684 398
Size: 286 5293
MluI A'CGCG_T
Cuts at: 5012 5012
Size: 5579
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1015 4250 4434 1015
Size: 3235 184 2160
Fragments arranged by size:
3235 2160 184
MscI TGG'CCA
Cuts at: 2140 2240 2539 3340 2140
Size: 100 299 801 4379
Fragments arranged by size:
4379 801 299 100
MslI CAynn'nnrTG
Cuts at: 558 827 1165 3568 3695 558
Size: 269 338 2403 127 2442
Fragments arranged by size:
2442 2403 338 269 127
MspA1I CmG'CkG
Cuts at: 1149 2092 2376 2690 3004 3364 4377 4622 1149
Size: 943 284 314 314 360 1013 245 2106
Fragments arranged by size:
2106 1013 943 360 314 314 284 245
NaeI GCC'GGC
Cuts at: 3760 3760
Size: 5579
NarI GG'CG_CC
Cuts at: 3010 3258 3010
Size: 248 5331
NcoI C'CATG_G
Cuts at: 140 140
Size: 5579
NdeI CA'TA_TG
Cuts at: 824 824
Size: 5579
NgoMIV G'CCGG_C
Cuts at: 3758 3758
Size: 5579
NspI r_CATG'y
Cuts at: 563 1114 2632 3132 4039 563
Size: 551 1518 500 907 2103
Fragments arranged by size:
2103 1518 907 551 500
PciI A'CATG_T
Cuts at: 1110 4035 1110
Size: 2925 2654
PflMI CCAn_nnn'nTGG
Cuts at: 2261 2261
Size: 5579
PfoI T'CCnGG_A
Cuts at: 115 115
Size: 5579
PmeI GTTT'AAAC
Cuts at: 2578 2578
Size: 5579
PmlI CAC'GTG
Cuts at: 2438 2438
Size: 5579
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4718 4750 4718
Size: 32 5547
PpuMI rG'GwC_Cy
Cuts at: 1977 1977
Size: 5579
PstI C_TGCA'G
Cuts at: 461 677 1317 2426 2626 3311 461
Size: 216 640 1109 200 685 2729
Fragments arranged by size:
2729 1109 685 640 216 200
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5579
PvuII CAG'CTG
Cuts at: 1149 2376 3004 3364 1149
Size: 1227 628 360 3364
Fragments arranged by size:
3364 1227 628 360
RsrII CG'GwC_CG
Cuts at: 3774 3774
Size: 5579
SacI G_AGCT'C
Cuts at: 1880 2593 1880
Size: 713 4866
SacII CC_GC'GG
Cuts at: 2093 2093
Size: 5579
SalI G'TCGA_C
Cuts at: 2616 2616
Size: 5579
SapI GCTCTTCn'nnn_
Cuts at: 3602 3812 3602
Size: 210 5369
SbfI CC_TGCA'GG
Cuts at: 2626 2626
Size: 5579
ScaI AGT'ACT
Cuts at: 1654 5096 1654
Size: 3442 2137
SexAI A'CCwGG_T
Cuts at: 809 809
Size: 5579
SfcI C'TryA_G
Cuts at: 33 457 673 759 1313 2226 2422 2622 3307
Size: 424 216 86 554 913 196 200 685
Cuts at: 3307 4300 4491 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 913 685 554 424 216 200
196 191 86
SfoI GGC'GCC
Cuts at: 3011 3259 3011
Size: 248 5331
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5579
SmaI CCC'GGG
Cuts at: 2203 2601 2203
Size: 398 5181
SmlI C'TyrA_G
Cuts at: 855 1468 1678 2734 3649 4141 4403 855
Size: 613 210 1056 915 492 262 2031
Fragments arranged by size:
2031 1056 915 613 492 262 210
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5579
SphI G_CATG'C
Cuts at: 2632 3132 2632
Size: 500 5079
SspI AAT'ATT
Cuts at: 657 1076 657
Size: 419 5160
StyI C'CwwG_G
Cuts at: 140 2540 2713 3067 140
Size: 2400 173 354 2652
Fragments arranged by size:
2652 2400 354 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1392 3162 3295 3754 3963 1392
Size: 1770 133 459 209 3008
Fragments arranged by size:
3008 1770 459 209 133
TatI w'GTAC_w
Cuts at: 629 1099 1652 1961 2210 2285 5094 629
Size: 470 553 309 249 75 2809 1114
Fragments arranged by size:
2809 1114 553 470 309 249 75
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5096 5128 5096
Size: 32 5547
Tth111I GACn'n_nGTC
Cuts at: 2055 3376 2055
Size: 1321 4258
XbaI T'CTAG_A
Cuts at: 2610 2610
Size: 5579
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1052 2151 3074 1052
Size: 1099 923 3557
Fragments arranged by size:
3557 1099 923
XmaI C'CCGG_G
Cuts at: 2201 2599 2201
Size: 398 5181
XmnI GAAnn'nnTTC
Cuts at: 508 508
Size: 5579
ZraI GAC'GTC
Cuts at: 1371 2313 1371
Size: 942 4637
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AhdI AleI AlfI AloI ApaI AscI AvrII BaeI
BcgI BfrBI BglI BmtI BsaXI BseRI BsiWI BsmBI
BssHII BstZ17I ClaI CspCI EcoRV FseI FspAI HpaI
NheI NotI NruI NsiI PacI PasI PshAI PsiI
PspOMI PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI