Restriction map for
pF1KB8458
(Circular) (Six-base) MAPSORT of: pF1KB8458.seq Check: 6852 from: 1 to: 4130
pF1KB8458 4130 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 522 522
Size: 4130
AatII G_ACGT'C
Cuts at: 494 494
Size: 4130
AccI GT'mk_AC
Cuts at: 386 1109 1168 386
Size: 723 59 3348
Fragments arranged by size:
3348 723 59
Acc65I G'GTAC_C
Cuts at: 1146 1146
Size: 4130
AclI AA'CG_TT
Cuts at: 3970 3970
Size: 4130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1955 2387 2740 3402 1955
Size: 432 353 662 2683
Fragments arranged by size:
2683 662 432 353
AfeI AGC'GCT
Cuts at: 322 1019 322
Size: 697 3433
AflIII A'CryG_T
Cuts at: 1482 3282 3563 1482
Size: 1800 281 2049
Fragments arranged by size:
2049 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 126 1435 2873 126
Size: 1309 1438 1383
Fragments arranged by size:
1438 1383 1309
ApaLI G'TGCA_C
Cuts at: 2968 2968
Size: 4130
ApoI r'AATT_y
Cuts at: 675 1134 3483 3739 675
Size: 459 2349 256 1066
Fragments arranged by size:
2349 1066 459 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4130
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4130
AvaI C'yCGr_G
Cuts at: 1150 1150
Size: 4130
BamHI G'GATC_C
Cuts at: 256 1155 256
Size: 899 3231
BanI G'GyrC_C
Cuts at: 534 1146 1560 1808 1843 534
Size: 612 414 248 35 2821
Fragments arranged by size:
2821 612 414 248 35
BanII G_rGCy'C
Cuts at: 926 1144 2174 926
Size: 218 1030 2882
Fragments arranged by size:
2882 1030 218
BbeI G_GCGC'C
Cuts at: 1564 1812 1564
Size: 248 3882
BbsI GAAGACnn'nnnn_
Cuts at: 250 851 250
Size: 601 3529
BbvCI CC'TCA_GC
Cuts at: 977 977
Size: 4130
BciVI GTATCCnnnnn_n'
Cuts at: 416 2028 3084 4130 416
Size: 1612 1056 1046 416
Fragments arranged by size:
1612 1056 1046 416
BclI T'GATC_A
Cuts at: 1650 1650
Size: 4130
BglI GCCn_nnn'nGGC
Cuts at: 175 175
Size: 4130
BglII A'GATC_T
Cuts at: 885 1645 3682 885
Size: 760 2037 1333
Fragments arranged by size:
2037 1333 760
BlpI GC'TnA_GC
Cuts at: 457 1242 457
Size: 785 3345
Bme1580I G_kGCm'C
Cuts at: 351 539 739 1755 1848 2972 351
Size: 188 200 1016 93 1124 1509
Fragments arranged by size:
1509 1124 1016 200 188 93
BmrI ACTGGGnnnn_n'
Cuts at: 1342 1423 1758 3902 1342
Size: 81 335 2144 1570
Fragments arranged by size:
2144 1570 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2497 2497
Size: 4130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 688 707 751 940 334
Size: 354 19 44 189 3524
Fragments arranged by size:
3524 354 189 44 19
Bpu10I CC'TnA_GC
Cuts at: 977 977
Size: 4130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1306 2185 2899 3197 1306
Size: 879 714 298 2239
Fragments arranged by size:
2239 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3834 3834
Size: 4130
BsaAI yAC'GTr
Cuts at: 390 1483 390
Size: 1093 3037
BsaBI GATnn'nnATC
Cuts at: 6 1644 3681 6
Size: 1638 2037 455
Fragments arranged by size:
2037 1638 455
BsaHI Gr'CG_yC
Cuts at: 491 1561 1809 2511 4007 491
Size: 1070 248 702 1496 614
Fragments arranged by size:
1496 1070 702 614 248
BsaWI w'CCGG_w
Cuts at: 156 494 1319 1840 2518 2929 3076 3350 156
Size: 338 825 521 678 411 147 274 936
Fragments arranged by size:
936 825 678 521 411 338 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 211 331 694 817 211
Size: 120 363 123 3524
Fragments arranged by size:
3524 363 123 120
BseYI C'CCAG_C
Cuts at: 119 375 1555 2313 2978 3386 119
Size: 256 1180 758 665 408 863
Fragments arranged by size:
1180 863 758 665 408 256
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1063
Size: 4130
BsiEI CG_ry'CG
Cuts at: 104 1672 1718 2948 104
Size: 1568 46 1230 1286
Fragments arranged by size:
1568 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 926 1144 1922 2112 2972 926
Size: 218 778 190 860 2084
Fragments arranged by size:
2084 860 778 218 190
Bsp1286I G_dGCh'C
Cuts at: 351 539 739 926 1144 1755 1848 1922 2112
Size: 188 200 187 218 611 93 74 190
Cuts at: 2112 2174 2972 351
Size: 62 798 1509
Fragments arranged by size:
1509 798 611 218 200 190 188 187
93 74 62
BspEI T'CCGG_A
Cuts at: 156 494 156
Size: 338 3792
BspHI T'CATG_A
Cuts at: 2562 2562
Size: 4130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 435 522 923 1180 1394 1696 2077 435
Size: 87 401 257 214 302 381 2488
Fragments arranged by size:
2488 401 381 302 257 214 87
BsrBI CCG'CTC
Cuts at: 50 2422 2476 3960 4125 50
Size: 2372 54 1484 165 55
Fragments arranged by size:
2372 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 182 2042 182
Size: 1860 2270
BsrFI r'CCGG_y
Cuts at: 465 551 2128 2309 465
Size: 86 1577 181 2286
Fragments arranged by size:
2286 1577 181 86
BssSI C'ACGA_G
Cuts at: 1037 2401 3109 1037
Size: 1364 708 2058
Fragments arranged by size:
2058 1364 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1507 1507
Size: 4130
BstBI TT'CG_AA
Cuts at: 2491 2491
Size: 4130
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4130
BstYI r'GATC_y
Cuts at: 1 256 497 782 885 984 1155 1622 1645
Size: 255 241 285 103 99 171 467 23
Cuts at: 1645 1980 2226 2532 2544 2630 3682 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 467 449 335 306 285 255 246
241 171 103 99 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 800 800
Size: 4130
BtgI C'CryG_G
Cuts at: 107 752 107
Size: 645 3485
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 855 2080 2261 2424 855
Size: 1225 181 163 2561
Fragments arranged by size:
2561 1225 181 163
BtsI GCAGTG_nn'
Cuts at: 351 1373 351
Size: 1022 3108
DraI TTT'AAA
Cuts at: 1129 1129
Size: 4130
DrdI GACnn_nn'nnGTC
Cuts at: 1836 3180 1836
Size: 1344 2786
EaeI y'GGCC_r
Cuts at: 176 1715 1889 2280 2307 3652 176
Size: 1539 174 391 27 1345 654
Fragments arranged by size:
1539 1345 654 391 174 27
EagI C'GGCC_G
Cuts at: 1715 1715
Size: 4130
EarI CTCTTCn'nnn_
Cuts at: 196 442 1069 2153 2363 196
Size: 246 627 1084 210 1963
Fragments arranged by size:
1963 1084 627 246 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 538 547 2338 3080 3226 3940 538
Size: 9 1791 742 146 714 728
Fragments arranged by size:
1791 742 728 714 146 9
EcoICRI GAG'CTC
Cuts at: 924 1142 924
Size: 218 3912
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 688 707 751 940 1955 2387 2740 3402
Size: 354 19 44 189 1015 432 353 662
Cuts at: 3402 334
Size: 1062
Fragments arranged by size:
1062 1015 662 432 354 353 189 44
19
EcoO109I rG'GnC_Cy
Cuts at: 1269 1269
Size: 4130
EcoRI G'AATT_C
Cuts at: 1134 1134
Size: 4130
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 225 1080 2149 225
Size: 855 1069 2206
Fragments arranged by size:
2206 1069 855
FspI TGC'GCA
Cuts at: 1911 1911
Size: 4130
HaeII r_GCGC'y
Cuts at: 324 1021 1062 1564 1812 3042 3813 324
Size: 697 41 502 248 1230 771 641
Fragments arranged by size:
1230 771 697 641 502 248 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 680 712 1972 2002 2004 2034 680
Size: 32 1260 30 2 30 2776
Fragments arranged by size:
2776 1260 32 30 30 2
HincII GTy'rAC
Cuts at: 609 1169 609
Size: 560 3570
KasI G'GCGC_C
Cuts at: 1560 1808 1560
Size: 248 3882
KpnI G_GTAC'C
Cuts at: 1150 1150
Size: 4130
MluI A'CGCG_T
Cuts at: 3563 3563
Size: 4130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 691 983 2889 3073 691
Size: 292 1906 184 1748
Fragments arranged by size:
1906 1748 292 184
MscI TGG'CCA
Cuts at: 1891 1891
Size: 4130
MslI CAynn'nnrTG
Cuts at: 112 238 874 2119 2246 112
Size: 126 636 1245 127 1996
Fragments arranged by size:
1996 1245 636 127 126
MspA1I CmG'CkG
Cuts at: 126 1241 1555 1915 2699 2944 126
Size: 1115 314 360 784 245 1312
Fragments arranged by size:
1312 1115 784 360 314 245
NaeI GCC'GGC
Cuts at: 553 2311 553
Size: 1758 2372
NarI GG'CG_CC
Cuts at: 1561 1809 1561
Size: 248 3882
NcoI C'CATG_G
Cuts at: 107 107
Size: 4130
NgoMIV G'CCGG_C
Cuts at: 551 2309 551
Size: 1758 2372
NspI r_CATG'y
Cuts at: 1183 1683 3286 1183
Size: 500 1603 2027
Fragments arranged by size:
2027 1603 500
PciI A'CATG_T
Cuts at: 3282 3282
Size: 4130
PflMI CCAn_nnn'nTGG
Cuts at: 821 821
Size: 4130
PmeI GTTT'AAAC
Cuts at: 1129 1129
Size: 4130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2576 2608 2576
Size: 32 4098
PstI C_TGCA'G
Cuts at: 1177 1862 1177
Size: 685 3445
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4130
PvuII CAG'CTG
Cuts at: 126 1555 1915 126
Size: 1429 360 2341
Fragments arranged by size:
2341 1429 360
RsrII CG'GwC_CG
Cuts at: 2325 2325
Size: 4130
SacI G_AGCT'C
Cuts at: 926 1144 926
Size: 218 3912
SalI G'TCGA_C
Cuts at: 1167 1167
Size: 4130
SapI GCTCTTCn'nnn_
Cuts at: 442 2153 2363 442
Size: 1711 210 2209
Fragments arranged by size:
2209 1711 210
SbfI CC_TGCA'GG
Cuts at: 1177 1177
Size: 4130
ScaI AGT'ACT
Cuts at: 3647 3647
Size: 4130
SfcI C'TryA_G
Cuts at: 33 1101 1173 1858 2826 3017 33
Size: 1068 72 685 968 191 1146
Fragments arranged by size:
1146 1068 968 685 191 72
SfoI GGC'GCC
Cuts at: 1562 1810 1562
Size: 248 3882
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4130
SmaI CCC'GGG
Cuts at: 1152 1152
Size: 4130
SmlI C'TyrA_G
Cuts at: 1285 2200 2914 3176 1285
Size: 915 714 262 2239
Fragments arranged by size:
2239 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4130
SphI G_CATG'C
Cuts at: 1183 1683 1183
Size: 500 3630
StyI C'CwwG_G
Cuts at: 107 1264 1618 107
Size: 1157 354 2619
Fragments arranged by size:
2619 1157 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 382 462 1713 1846 2305 2514 382
Size: 80 1251 133 459 209 1998
Fragments arranged by size:
1998 1251 459 209 133 80
TatI w'GTAC_w
Cuts at: 3645 3645
Size: 4130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3647 3679 3647
Size: 32 4098
Tth111I GACn'n_nGTC
Cuts at: 1927 1927
Size: 4130
XbaI T'CTAG_A
Cuts at: 1161 1161
Size: 4130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 387 1625 387
Size: 1238 2892
XmaI C'CCGG_G
Cuts at: 1150 1150
Size: 4130
XmnI GAAnn'nnTTC
Cuts at: 152 152
Size: 4130
ZraI GAC'GTC
Cuts at: 492 492
Size: 4130
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI