Restriction map for
pF1KB8451
(Circular) (Six-base) MAPSORT of: pF1KB8451.seq Check: 3528 from: 1 to: 4205
pF1KB8451 4205 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AatII G_ACGT'C
Cuts at: 374 374
Size: 4205
AccI GT'mk_AC
Cuts at: 1243 1243
Size: 4205
Acc65I G'GTAC_C
Cuts at: 668 1221 668
Size: 553 3652
AclI AA'CG_TT
Cuts at: 4045 4045
Size: 4205
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 387 541 580 823 1014 2030 2462 2815
Size: 26 154 39 243 191 1016 432 353
Cuts at: 2815 3477 361
Size: 662 1089
Fragments arranged by size:
1089 1016 662 432 353 243 191 154
39 26
AflIII A'CryG_T
Cuts at: 1557 3357 3638 1557
Size: 1800 281 2124
Fragments arranged by size:
2124 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 144 177 186 291 471 510 891 1510 2948
Size: 33 9 105 180 39 381 619 1438
Cuts at: 2948 144
Size: 1401
Fragments arranged by size:
1438 1401 619 381 180 105 39 33
9
ApaI G_GGCC'C
Cuts at: 118 118
Size: 4205
ApaLI G'TGCA_C
Cuts at: 3043 3043
Size: 4205
ApoI r'AATT_y
Cuts at: 1209 3558 3814 1209
Size: 2349 256 1600
Fragments arranged by size:
2349 1600 256
AscI GG'CGCG_CC
Cuts at: 622 622
Size: 4205
AseI AT'TA_AT
Cuts at: 20 20
Size: 4205
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4205
AvaI C'yCGr_G
Cuts at: 777 1048 1225 777
Size: 271 177 3757
Fragments arranged by size:
3757 271 177
BamHI G'GATC_C
Cuts at: 602 1230 602
Size: 628 3577
BanI G'GyrC_C
Cuts at: 165 205 436 668 675 1221 1635 1883 1918
Size: 40 231 232 7 546 414 248 35
Cuts at: 1918 165
Size: 2452
Fragments arranged by size:
2452 546 414 248 232 231 40 35
7
BanII G_rGCy'C
Cuts at: 118 202 599 1127 1219 2249 118
Size: 84 397 528 92 1030 2074
Fragments arranged by size:
2074 1030 528 397 92 84
BbeI G_GCGC'C
Cuts at: 209 440 1639 1887 209
Size: 231 1199 248 2527
Fragments arranged by size:
2527 1199 248 231
BbsI GAAGACnn'nnnn_
Cuts at: 366 366
Size: 4205
BbvCI CC'TCA_GC
Cuts at: 1007 1144 1007
Size: 137 4068
BciVI GTATCCnnnnn_n'
Cuts at: 2103 3159 4205 2103
Size: 1056 1046 2103
Fragments arranged by size:
2103 1056 1046
BclI T'GATC_A
Cuts at: 1725 1725
Size: 4205
BfrBI ATG'CAT
Cuts at: 702 702
Size: 4205
BglI GCCn_nnn'nGGC
Cuts at: 174 197 174
Size: 23 4182
BglII A'GATC_T
Cuts at: 1720 3757 1720
Size: 2037 2168
BlpI GC'TnA_GC
Cuts at: 472 749 1317 472
Size: 277 568 3360
Fragments arranged by size:
3360 568 277
Bme1580I G_kGCm'C
Cuts at: 118 678 829 1830 1923 3047 118
Size: 560 151 1001 93 1124 1276
Fragments arranged by size:
1276 1124 1001 560 151 93
BmgBI CAC'GTC
Cuts at: 389 389
Size: 4205
BmrI ACTGGGnnnn_n'
Cuts at: 1417 1498 1833 3977 1417
Size: 81 335 2144 1645
Fragments arranged by size:
2144 1645 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2572 2572
Size: 4205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 1129 1207 742
Size: 387 78 3740
Fragments arranged by size:
3740 387 78
Bpu10I CC'TnA_GC
Cuts at: 598 916 1007 1144 598
Size: 318 91 137 3659
Fragments arranged by size:
3659 318 137 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 1381 2260 2974 3272 316
Size: 1065 879 714 298 1249
Fragments arranged by size:
1249 1065 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3909 3909
Size: 4205
BsaAI yAC'GTr
Cuts at: 1558 1558
Size: 4205
BsaBI GATnn'nnATC
Cuts at: 6 1719 3756 6
Size: 1713 2037 455
Fragments arranged by size:
2037 1713 455
BsaHI Gr'CG_yC
Cuts at: 206 236 371 437 1636 1884 2586 4082 206
Size: 30 135 66 1199 248 702 1496 329
Fragments arranged by size:
1496 1199 702 329 248 135 66 30
BsaWI w'CCGG_w
Cuts at: 1394 1915 2593 3004 3151 3425 1394
Size: 521 678 411 147 274 2174
Fragments arranged by size:
2174 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 652 849 1126 652
Size: 197 277 3731
Fragments arranged by size:
3731 277 197
BseYI C'CCAG_C
Cuts at: 565 832 1630 2388 3053 3461 565
Size: 267 798 758 665 408 1309
Fragments arranged by size:
1309 798 758 665 408 267
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1022 1022
Size: 4205
BsiEI CG_ry'CG
Cuts at: 104 539 1747 1793 3023 104
Size: 435 1208 46 1230 1286
Fragments arranged by size:
1286 1230 1208 435 46
BsiHKAI G_wGCw'C
Cuts at: 485 599 1127 1219 1997 2187 3047 485
Size: 114 528 92 778 190 860 1643
Fragments arranged by size:
1643 860 778 528 190 114 92
Bsp1286I G_dGCh'C
Cuts at: 118 202 485 599 678 829 1127 1219 1830
Size: 84 283 114 79 151 298 92 611
Cuts at: 1830 1923 1997 2187 2249 3047 118
Size: 93 74 190 62 798 1276
Fragments arranged by size:
1276 798 611 298 283 190 151 114
93 92 84 79 74 62
BspHI T'CATG_A
Cuts at: 2637 2637
Size: 4205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1188 1255 1469 1771 2152 1188
Size: 67 214 302 381 3241
Fragments arranged by size:
3241 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 2497 2551 4035 4200 50
Size: 2447 54 1484 165 55
Fragments arranged by size:
2447 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2117 2117
Size: 4205
BsrFI r'CCGG_y
Cuts at: 124 134 381 646 2203 2384 124
Size: 10 247 265 1557 181 1945
Fragments arranged by size:
1945 1557 265 247 181 10
BssHII G'CGCG_C
Cuts at: 301 622 301
Size: 321 3884
BssSI C'ACGA_G
Cuts at: 346 2476 3184 346
Size: 2130 708 1367
Fragments arranged by size:
2130 1367 708
BstAPI GCAn_nnn'nTGC
Cuts at: 177 186 1582 177
Size: 9 1396 2800
Fragments arranged by size:
2800 1396 9
BstBI TT'CG_AA
Cuts at: 2566 2566
Size: 4205
BstEII G'GTnAC_C
Cuts at: 420 420
Size: 4205
BstXI CCAn_nnnn'nTGG
Cuts at: 432 573 945 432
Size: 141 372 3692
Fragments arranged by size:
3692 372 141
BstYI r'GATC_y
Cuts at: 1 602 1230 1697 1720 2055 2301 2607 2619
Size: 601 628 467 23 335 246 306 12
Cuts at: 2619 2705 3757 1
Size: 86 1052 449
Fragments arranged by size:
1052 628 601 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 839 839
Size: 4205
BtgI C'CryG_G
Cuts at: 731 930 731
Size: 199 4006
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2155 2336 2499 2155
Size: 181 163 3861
Fragments arranged by size:
3861 181 163
BtsI GCAGTG_nn'
Cuts at: 1448 1448
Size: 4205
DraI TTT'AAA
Cuts at: 1204 1204
Size: 4205
DrdI GACnn_nn'nnGTC
Cuts at: 994 1911 3255 994
Size: 917 1344 1944
Fragments arranged by size:
1944 1344 917
EaeI y'GGCC_r
Cuts at: 383 536 656 947 1790 1964 2355 2382 3727
Size: 153 120 291 843 174 391 27 1345
Cuts at: 3727 383
Size: 861
Fragments arranged by size:
1345 861 843 391 291 174 153 120
27
EagI C'GGCC_G
Cuts at: 536 1790 536
Size: 1254 2951
EarI CTCTTCn'nnn_
Cuts at: 778 2228 2438 778
Size: 1450 210 2545
Fragments arranged by size:
2545 1450 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 646 2413 3155 3301 4015 646
Size: 1767 742 146 714 836
Fragments arranged by size:
1767 836 742 714 146
EcoICRI GAG'CTC
Cuts at: 597 1125 1217 597
Size: 528 92 3585
Fragments arranged by size:
3585 528 92
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 387 541 580 742 823 1014 1129 1207
Size: 26 154 39 162 81 191 115 78
Cuts at: 1207 2030 2462 2815 3477 361
Size: 823 432 353 662 1089
Fragments arranged by size:
1089 823 662 432 353 191 162 154
115 81 78 39 26
EcoO109I rG'GnC_Cy
Cuts at: 115 555 768 912 1344 115
Size: 440 213 144 432 2976
Fragments arranged by size:
2976 440 432 213 144
EcoRI G'AATT_C
Cuts at: 1209 1209
Size: 4205
EcoRV GAT'ATC
Cuts at: 723 723
Size: 4205
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 789 2224 789
Size: 1435 2770
FspI TGC'GCA
Cuts at: 1986 1986
Size: 4205
HaeII r_GCGC'y
Cuts at: 209 440 472 1639 1887 3117 3888 209
Size: 231 32 1167 248 1230 771 526
Fragments arranged by size:
1230 1167 771 526 248 231 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 712 744 2047 2077 2079 2109 712
Size: 32 1303 30 2 30 2808
Fragments arranged by size:
2808 1303 32 30 30 2
HincII GTy'rAC
Cuts at: 1244 1244
Size: 4205
KasI G'GCGC_C
Cuts at: 205 436 1635 1883 205
Size: 231 1199 248 2527
Fragments arranged by size:
2527 1199 248 231
KpnI G_GTAC'C
Cuts at: 672 1225 672
Size: 553 3652
MluI A'CGCG_T
Cuts at: 3638 3638
Size: 4205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2964 3148 2964
Size: 184 4021
MscI TGG'CCA
Cuts at: 658 949 1966 658
Size: 291 1017 2897
Fragments arranged by size:
2897 1017 291
MslI CAynn'nnrTG
Cuts at: 430 817 2194 2321 430
Size: 387 1377 127 2314
Fragments arranged by size:
2314 1377 387 127
MspA1I CmG'CkG
Cuts at: 174 507 801 1006 1316 1630 1990 2774 3019
Size: 333 294 205 310 314 360 784 245
Cuts at: 3019 174
Size: 1360
Fragments arranged by size:
1360 784 360 333 314 310 294 245
205
NaeI GCC'GGC
Cuts at: 126 383 2386 126
Size: 257 2003 1945
Fragments arranged by size:
2003 1945 257
NarI GG'CG_CC
Cuts at: 206 437 1636 1884 206
Size: 231 1199 248 2527
Fragments arranged by size:
2527 1199 248 231
NdeI CA'TA_TG
Cuts at: 1074 1074
Size: 4205
NgoMIV G'CCGG_C
Cuts at: 124 381 2384 124
Size: 257 2003 1945
Fragments arranged by size:
2003 1945 257
NsiI A_TGCA'T
Cuts at: 704 704
Size: 4205
NspI r_CATG'y
Cuts at: 1258 1758 3361 1258
Size: 500 1603 2102
Fragments arranged by size:
2102 1603 500
PciI A'CATG_T
Cuts at: 3357 3357
Size: 4205
PflMI CCAn_nnn'nTGG
Cuts at: 255 665 717 255
Size: 410 52 3743
Fragments arranged by size:
3743 410 52
PfoI T'CCnGG_A
Cuts at: 605 605
Size: 4205
PmeI GTTT'AAAC
Cuts at: 1204 1204
Size: 4205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2651 2683 2651
Size: 32 4173
PpuMI rG'GwC_Cy
Cuts at: 555 912 555
Size: 357 3848
PspOMI G'GGCC_C
Cuts at: 114 114
Size: 4205
PstI C_TGCA'G
Cuts at: 182 1252 1937 182
Size: 1070 685 2450
Fragments arranged by size:
2450 1070 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4205
PvuII CAG'CTG
Cuts at: 507 801 1006 1630 1990 507
Size: 294 205 624 360 2722
Fragments arranged by size:
2722 624 360 294 205
RsrII CG'GwC_CG
Cuts at: 2400 2400
Size: 4205
SacI G_AGCT'C
Cuts at: 599 1127 1219 599
Size: 528 92 3585
Fragments arranged by size:
3585 528 92
SalI G'TCGA_C
Cuts at: 1242 1242
Size: 4205
SapI GCTCTTCn'nnn_
Cuts at: 2228 2438 2228
Size: 210 3995
SbfI CC_TGCA'GG
Cuts at: 1252 1252
Size: 4205
ScaI AGT'ACT
Cuts at: 3722 3722
Size: 4205
SfcI C'TryA_G
Cuts at: 33 178 1248 1933 2901 3092 33
Size: 145 1070 685 968 191 1146
Fragments arranged by size:
1146 1070 968 685 191 145
SfoI GGC'GCC
Cuts at: 207 438 1637 1885 207
Size: 231 1199 248 2527
Fragments arranged by size:
2527 1199 248 231
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4205
SmaI CCC'GGG
Cuts at: 779 1227 779
Size: 448 3757
SmlI C'TyrA_G
Cuts at: 331 1360 2275 2989 3251 331
Size: 1029 915 714 262 1285
Fragments arranged by size:
1285 1029 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4205
SphI G_CATG'C
Cuts at: 1258 1758 1258
Size: 500 3705
StyI C'CwwG_G
Cuts at: 960 1094 1339 1693 960
Size: 134 245 354 3472
Fragments arranged by size:
3472 354 245 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 846 1788 1921 2380 2589 846
Size: 942 133 459 209 2462
Fragments arranged by size:
2462 942 459 209 133
TatI w'GTAC_w
Cuts at: 413 524 3720 413
Size: 111 3196 898
Fragments arranged by size:
3196 898 111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3722 3754 3722
Size: 32 4173
Tth111I GACn'n_nGTC
Cuts at: 2002 2002
Size: 4205
XbaI T'CTAG_A
Cuts at: 1236 1236
Size: 4205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1700 1700
Size: 4205
XmaI C'CCGG_G
Cuts at: 777 1225 777
Size: 448 3757
ZraI GAC'GTC
Cuts at: 372 372
Size: 4205
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NsiI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AleI AlfI AloI
AvrII BaeI BcgI BmtI BsaXI BsiWI BsmI BsmBI
BspEI BsrGI BstZ17I ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NcoI NheI NotI NruI
PacI PasI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI