Restriction map for
pF1KB8447
(Circular) (Six-base) MAPSORT of: pF1KB8447.seq Check: 4417 from: 1 to: 4520
pF1KB8447 4520 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1558 1558
Size: 4520
Acc65I G'GTAC_C
Cuts at: 1077 1536 1077
Size: 459 4061
AclI AA'CG_TT
Cuts at: 4360 4360
Size: 4520
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 997 2345 2777 3130 3792 227
Size: 770 1348 432 353 662 955
Fragments arranged by size:
1348 955 770 662 432 353
AflIII A'CryG_T
Cuts at: 252 421 1872 3672 3953 252
Size: 169 1451 1800 281 819
Fragments arranged by size:
1800 1451 819 281 169
AlwNI CAG_nnn'CTG
Cuts at: 960 1825 3263 960
Size: 865 1438 2217
Fragments arranged by size:
2217 1438 865
ApaI G_GGCC'C
Cuts at: 1339 1339
Size: 4520
ApaLI G'TGCA_C
Cuts at: 118 3358 118
Size: 3240 1280
ApoI r'AATT_y
Cuts at: 187 221 389 666 909 1524 3873 4129 187
Size: 34 168 277 243 615 2349 256 578
Fragments arranged by size:
2349 615 578 277 256 243 168 34
AseI AT'TA_AT
Cuts at: 20 20
Size: 4520
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4520
AvaI C'yCGr_G
Cuts at: 1540 1540
Size: 4520
AvrII C'CTAG_G
Cuts at: 1191 1191
Size: 4520
BamHI G'GATC_C
Cuts at: 1545 1545
Size: 4520
BanI G'GyrC_C
Cuts at: 1077 1536 1950 2198 2233 1077
Size: 459 414 248 35 3364
Fragments arranged by size:
3364 459 414 248 35
BanII G_rGCy'C
Cuts at: 715 1339 1534 2564 715
Size: 624 195 1030 2671
Fragments arranged by size:
2671 1030 624 195
BbeI G_GCGC'C
Cuts at: 1954 2202 1954
Size: 248 4272
BbsI GAAGACnn'nnnn_
Cuts at: 775 775
Size: 4520
BciVI GTATCCnnnnn_n'
Cuts at: 2418 3474 4520 2418
Size: 1056 1046 2418
Fragments arranged by size:
2418 1056 1046
BclI T'GATC_A
Cuts at: 2040 2040
Size: 4520
BglI GCCn_nnn'nGGC
Cuts at: 1262 1262
Size: 4520
BglII A'GATC_T
Cuts at: 2035 4072 2035
Size: 2037 2483
BlpI GC'TnA_GC
Cuts at: 1632 1632
Size: 4520
Bme1580I G_kGCm'C
Cuts at: 122 1339 2145 2238 3362 122
Size: 1217 806 93 1124 1280
Fragments arranged by size:
1280 1217 1124 806 93
BmrI ACTGGGnnnn_n'
Cuts at: 820 1218 1732 1813 2148 4292 820
Size: 398 514 81 335 2144 1048
Fragments arranged by size:
2144 1048 514 398 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2887 2887
Size: 4520
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 807 1696 2575 3289 3587 532
Size: 275 889 879 714 298 1465
Fragments arranged by size:
1465 889 879 714 298 275
BsaI GGTCTCn'nnnn_
Cuts at: 4224 4224
Size: 4520
BsaAI yAC'GTr
Cuts at: 1873 1873
Size: 4520
BsaBI GATnn'nnATC
Cuts at: 6 2034 4071 6
Size: 2028 2037 455
Fragments arranged by size:
2037 2028 455
BsaHI Gr'CG_yC
Cuts at: 832 1484 1951 2199 2901 4397 832
Size: 652 467 248 702 1496 955
Fragments arranged by size:
1496 955 702 652 467 248
BsaWI w'CCGG_w
Cuts at: 1709 2230 2908 3319 3466 3740 1709
Size: 521 678 411 147 274 2489
Fragments arranged by size:
2489 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 498 498
Size: 4520
BseYI C'CCAG_C
Cuts at: 1498 1945 2703 3368 3776 1498
Size: 447 758 665 408 2242
Fragments arranged by size:
2242 758 665 447 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 593 1207 593
Size: 614 3906
BsiEI CG_ry'CG
Cuts at: 104 2062 2108 3338 104
Size: 1958 46 1230 1286
Fragments arranged by size:
1958 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 122 715 1534 2312 2502 3362 122
Size: 593 819 778 190 860 1280
Fragments arranged by size:
1280 860 819 778 593 190
BsiWI C'GTAC_G
Cuts at: 1382 1382
Size: 4520
BsmBI CGTCTCn'nnnn_
Cuts at: 718 718
Size: 4520
Bsp1286I G_dGCh'C
Cuts at: 122 715 1339 1534 2145 2238 2312 2502 2564
Size: 593 624 195 611 93 74 190 62
Cuts at: 2564 3362 122
Size: 798 1280
Fragments arranged by size:
1280 798 624 611 593 195 190 93
74 62
BspHI T'CATG_A
Cuts at: 357 2952 357
Size: 2595 1925
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1570 1784 2086 2467 1570
Size: 214 302 381 3623
Fragments arranged by size:
3623 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2812 2866 4350 4515 50
Size: 2762 54 1484 165 55
Fragments arranged by size:
2762 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1103 2432 1103
Size: 1329 3191
BsrFI r'CCGG_y
Cuts at: 1213 2518 2699 1213
Size: 1305 181 3034
Fragments arranged by size:
3034 1305 181
BssSI C'ACGA_G
Cuts at: 2791 3499 2791
Size: 708 3812
BstAPI GCAn_nnn'nTGC
Cuts at: 1897 1897
Size: 4520
BstBI TT'CG_AA
Cuts at: 659 846 2881 659
Size: 187 2035 2298
Fragments arranged by size:
2298 2035 187
BstEII G'GTnAC_C
Cuts at: 582 582
Size: 4520
BstYI r'GATC_y
Cuts at: 1 1545 2012 2035 2370 2616 2922 2934 3020
Size: 1544 467 23 335 246 306 12 86
Cuts at: 3020 4072 1
Size: 1052 449
Fragments arranged by size:
1544 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1402 1402
Size: 4520
BtgI C'CryG_G
Cuts at: 107 509 643 890 1143 1448 107
Size: 402 134 247 253 305 3179
Fragments arranged by size:
3179 402 305 253 247 134
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1401 2470 2651 2814 1401
Size: 1069 181 163 3107
Fragments arranged by size:
3107 1069 181 163
BtsI GCAGTG_nn'
Cuts at: 715 1763 715
Size: 1048 3472
ClaI AT'CG_AT
Cuts at: 899 899
Size: 4520
DraI TTT'AAA
Cuts at: 1519 1519
Size: 4520
DrdI GACnn_nn'nnGTC
Cuts at: 2226 3570 2226
Size: 1344 3176
EaeI y'GGCC_r
Cuts at: 1249 2105 2279 2670 2697 4042 1249
Size: 856 174 391 27 1345 1727
Fragments arranged by size:
1727 1345 856 391 174 27
EagI C'GGCC_G
Cuts at: 2105 2105
Size: 4520
EarI CTCTTCn'nnn_
Cuts at: 146 2543 2753 146
Size: 2397 210 1913
Fragments arranged by size:
2397 1913 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1082 2728 3470 3616 4330 1082
Size: 1646 742 146 714 1272
Fragments arranged by size:
1646 1272 742 714 146
EcoICRI GAG'CTC
Cuts at: 713 1532 713
Size: 819 3701
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 997 2345 2777 3130 3792 227
Size: 770 1348 432 353 662 955
Fragments arranged by size:
1348 955 770 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 337 337
Size: 4520
EcoO109I rG'GnC_Cy
Cuts at: 1129 1335 1336 1659 1129
Size: 206 1 323 3990
Fragments arranged by size:
3990 323 206 1
EcoRI G'AATT_C
Cuts at: 909 1524 909
Size: 615 3905
EcoRV GAT'ATC
Cuts at: 291 291
Size: 4520
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 603 2539 603
Size: 1936 2584
FspI TGC'GCA
Cuts at: 2301 2301
Size: 4520
HaeII r_GCGC'y
Cuts at: 1954 2202 3432 4203 1954
Size: 248 1230 771 2271
Fragments arranged by size:
2271 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1081 1113 2362 2392 2394 2424 1081
Size: 32 1249 30 2 30 3177
Fragments arranged by size:
3177 1249 32 30 30 2
HincII GTy'rAC
Cuts at: 951 1559 951
Size: 608 3912
HindIII A'AGCT_T
Cuts at: 550 550
Size: 4520
HpaI GTT'AAC
Cuts at: 951 951
Size: 4520
KasI G'GCGC_C
Cuts at: 1950 2198 1950
Size: 248 4272
KpnI G_GTAC'C
Cuts at: 1081 1540 1081
Size: 459 4061
MluI A'CGCG_T
Cuts at: 3953 3953
Size: 4520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 495 1406 3279 3463 495
Size: 911 1873 184 1552
Fragments arranged by size:
1873 1552 911 184
MscI TGG'CCA
Cuts at: 2281 2281
Size: 4520
MslI CAynn'nnrTG
Cuts at: 263 2509 2636 263
Size: 2246 127 2147
Fragments arranged by size:
2246 2147 127
MspA1I CmG'CkG
Cuts at: 745 1145 1376 1631 1945 2305 3089 3334 745
Size: 400 231 255 314 360 784 245 1931
Fragments arranged by size:
1931 784 400 360 314 255 245 231
NaeI GCC'GGC
Cuts at: 1215 2701 1215
Size: 1486 3034
NarI GG'CG_CC
Cuts at: 1951 2199 1951
Size: 248 4272
NcoI C'CATG_G
Cuts at: 107 509 890 1448 107
Size: 402 381 558 3179
Fragments arranged by size:
3179 558 402 381
NdeI CA'TA_TG
Cuts at: 763 763
Size: 4520
NgoMIV G'CCGG_C
Cuts at: 1213 2699 1213
Size: 1486 3034
NspI r_CATG'y
Cuts at: 256 425 1158 1573 2073 3676 256
Size: 169 733 415 500 1603 1100
Fragments arranged by size:
1603 1100 733 500 415 169
PciI A'CATG_T
Cuts at: 252 421 3672 252
Size: 169 3251 1100
Fragments arranged by size:
3251 1100 169
PflMI CCAn_nnn'nTGG
Cuts at: 151 960 151
Size: 809 3711
PmeI GTTT'AAAC
Cuts at: 1519 1519
Size: 4520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2966 2998 2966
Size: 32 4488
PpuMI rG'GwC_Cy
Cuts at: 1129 1129
Size: 4520
PshAI GACnn'nnGTC
Cuts at: 422 422
Size: 4520
PsiI TTA'TAA
Cuts at: 503 503
Size: 4520
PspOMI G'GGCC_C
Cuts at: 1335 1335
Size: 4520
PstI C_TGCA'G
Cuts at: 1567 2252 1567
Size: 685 3835
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4520
PvuII CAG'CTG
Cuts at: 745 1945 2305 745
Size: 1200 360 2960
Fragments arranged by size:
2960 1200 360
RsrII CG'GwC_CG
Cuts at: 2715 2715
Size: 4520
SacI G_AGCT'C
Cuts at: 715 1534 715
Size: 819 3701
SacII CC_GC'GG
Cuts at: 1146 1146
Size: 4520
SalI G'TCGA_C
Cuts at: 1557 1557
Size: 4520
SanDI GG'GwC_CC
Cuts at: 1129 1129
Size: 4520
SapI GCTCTTCn'nnn_
Cuts at: 2543 2753 2543
Size: 210 4310
SbfI CC_TGCA'GG
Cuts at: 1567 1567
Size: 4520
ScaI AGT'ACT
Cuts at: 4037 4037
Size: 4520
SfcI C'TryA_G
Cuts at: 33 1563 2248 3216 3407 33
Size: 1530 685 968 191 1146
Fragments arranged by size:
1530 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1952 2200 1952
Size: 248 4272
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4520
SmaI CCC'GGG
Cuts at: 1542 1542
Size: 4520
SmlI C'TyrA_G
Cuts at: 547 822 1675 2590 3304 3566 547
Size: 275 853 915 714 262 1501
Fragments arranged by size:
1501 915 853 714 275 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4520
SphI G_CATG'C
Cuts at: 1158 1573 2073 1158
Size: 415 500 3605
Fragments arranged by size:
3605 500 415
StyI C'CwwG_G
Cuts at: 107 509 706 890 925 1122 1191 1448 1654
Size: 402 197 184 35 197 69 257 206
Cuts at: 1654 2008 107
Size: 354 2619
Fragments arranged by size:
2619 402 354 257 206 197 197 184
69 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2103 2236 2695 2904 2103
Size: 133 459 209 3719
Fragments arranged by size:
3719 459 209 133
TatI w'GTAC_w
Cuts at: 749 1455 4035 749
Size: 706 2580 1234
Fragments arranged by size:
2580 1234 706
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4037 4069 4037
Size: 32 4488
Tth111I GACn'n_nGTC
Cuts at: 831 2317 831
Size: 1486 3034
XbaI T'CTAG_A
Cuts at: 1551 1551
Size: 4520
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1264 1495 2015 1264
Size: 231 520 3769
Fragments arranged by size:
3769 520 231
XmaI C'CCGG_G
Cuts at: 1540 1540
Size: 4520
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AscI BaeI BbvCI BcgI BfrBI BmgBI BmtI
BpmI Bpu10I BsaXI BsmI BspEI BsrGI BssHII BstXI
BstZ17I CspCI DraIII FseI FspAI MfeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PspXI PsrI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI