Restriction map for
pF1KB8440
(Circular) (Six-base) MAPSORT of: pF1KB8440.seq Check: 4284 from: 1 to: 4376
pF1KB8440 4376 bp
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 820 820
Size: 4376
AccI GT'mk_AC
Cuts at: 1414 1414
Size: 4376
Acc65I G'GTAC_C
Cuts at: 314 1392 314
Size: 1078 3298
AclI AA'CG_TT
Cuts at: 4216 4216
Size: 4376
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2201 2633 3380 3648 2201
Size: 432 747 268 2929
Fragments arranged by size:
2929 747 432 268
AflIII A'CryG_T
Cuts at: 178 322 705 1728 2832 3809 178
Size: 144 383 1023 1104 977 745
Fragments arranged by size:
1104 1023 977 745 383 144
AgeI A'CCGG_T
Cuts at: 2764 2764
Size: 4376
AleI CACnn'nnGTG
Cuts at: 323 323
Size: 4376
AlwNI CAG_nnn'CTG
Cuts at: 1681 3248 1681
Size: 1567 2809
ApaLI G'TGCA_C
Cuts at: 3146 3146
Size: 4376
ApoI r'AATT_y
Cuts at: 342 1380 3729 3985 342
Size: 1038 2349 256 733
Fragments arranged by size:
2349 1038 733 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4376
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4376
AvaI C'yCGr_G
Cuts at: 1396 1396
Size: 4376
BamHI G'GATC_C
Cuts at: 1401 1401
Size: 4376
BanI G'GyrC_C
Cuts at: 314 459 1392 1806 2054 2089 314
Size: 145 933 414 248 35 2601
Fragments arranged by size:
2601 933 414 248 145 35
BanII G_rGCy'C
Cuts at: 1349 1390 2420 1349
Size: 41 1030 3305
Fragments arranged by size:
3305 1030 41
BbeI G_GCGC'C
Cuts at: 463 1810 2058 463
Size: 1347 248 2781
Fragments arranged by size:
2781 1347 248
BbsI GAAGACnn'nnnn_
Cuts at: 1306 1306
Size: 4376
BciVI GTATCCnnnnn_n'
Cuts at: 800 2274 3035 4376 800
Size: 1474 761 1341 800
Fragments arranged by size:
1474 1341 800 761
BclI T'GATC_A
Cuts at: 1896 1896
Size: 4376
BglII A'GATC_T
Cuts at: 290 1891 3928 290
Size: 1601 2037 738
Fragments arranged by size:
2037 1601 738
BlpI GC'TnA_GC
Cuts at: 1488 1488
Size: 4376
Bme1580I G_kGCm'C
Cuts at: 909 2001 2094 3150 909
Size: 1092 93 1056 2135
Fragments arranged by size:
2135 1092 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 738 1588 1669 2004 4148 738
Size: 850 81 335 2144 966
Fragments arranged by size:
2144 966 850 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2743 2743
Size: 4376
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 423 1160 271
Size: 152 737 3487
Fragments arranged by size:
3487 737 152
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1552 2431 2923 3221 1552
Size: 879 492 298 2707
Fragments arranged by size:
2707 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1330 4080 1330
Size: 2750 1626
BsaAI yAC'GTr
Cuts at: 179 299 706 1729 179
Size: 120 407 1023 2826
Fragments arranged by size:
2826 1023 407 120
BsaBI GATnn'nnATC
Cuts at: 6 1890 3927 6
Size: 1884 2037 455
Fragments arranged by size:
2037 1884 455
BsaHI Gr'CG_yC
Cuts at: 460 1807 2055 2757 4253 460
Size: 1347 248 702 1496 583
Fragments arranged by size:
1496 1347 702 583 248
BsaWI w'CCGG_w
Cuts at: 1565 2086 2764 3038 3185 3596 1565
Size: 521 678 274 147 411 2345
Fragments arranged by size:
2345 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 638 650 890 1133 1801 2559 3136 3632 638
Size: 12 240 243 668 758 577 496 1382
Fragments arranged by size:
1382 758 668 577 496 243 240 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 236 236
Size: 4376
BsiEI CG_ry'CG
Cuts at: 104 1033 1918 1964 3172 104
Size: 929 885 46 1208 1308
Fragments arranged by size:
1308 1208 929 885 46
BsiHKAI G_wGCw'C
Cuts at: 359 1349 1390 2168 2358 3150 359
Size: 990 41 778 190 792 1585
Fragments arranged by size:
1585 990 792 778 190 41
Bsp1286I G_dGCh'C
Cuts at: 359 909 1349 1390 2001 2094 2168 2358 2420
Size: 550 440 41 611 93 74 190 62
Cuts at: 2420 3150 359
Size: 730 1585
Fragments arranged by size:
1585 730 611 550 440 190 93 74
62 41
BspEI T'CCGG_A
Cuts at: 3596 3596
Size: 4376
BspHI T'CATG_A
Cuts at: 498 3552 498
Size: 3054 1322
BspMI ACCTGCnnnn'nnnn_
Cuts at: 820 1426 1640 1942 2323 820
Size: 606 214 302 381 2873
Fragments arranged by size:
2873 606 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2668 2722 4206 4371 50
Size: 2618 54 1484 165 55
Fragments arranged by size:
2618 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 533 2288 533
Size: 1755 2621
BsrFI r'CCGG_y
Cuts at: 646 1125 2374 2555 2764 646
Size: 479 1249 181 209 2258
Fragments arranged by size:
2258 1249 479 209 181
BssSI C'ACGA_G
Cuts at: 541 2647 3005 541
Size: 2106 358 1912
Fragments arranged by size:
2106 1912 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1753 1753
Size: 4376
BstBI TT'CG_AA
Cuts at: 2737 2737
Size: 4376
BstXI CCAn_nnnn'nTGG
Cuts at: 138 325 138
Size: 187 4189
BstYI r'GATC_y
Cuts at: 1 290 711 1401 1868 1891 2226 2472 3484
Size: 289 421 690 467 23 335 246 1012
Cuts at: 3484 3570 3582 3928 1
Size: 86 12 346 449
Fragments arranged by size:
1012 690 467 449 421 346 335 289
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 155 107
Size: 48 4328
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2326 2507 2670 2326
Size: 181 163 4032
Fragments arranged by size:
4032 181 163
BtsI GCAGTG_nn'
Cuts at: 155 359 369 1619 155
Size: 204 10 1250 2912
Fragments arranged by size:
2912 1250 204 10
DraI TTT'AAA
Cuts at: 1375 1375
Size: 4376
DraIII CAC_nnn'GTG
Cuts at: 223 223
Size: 4376
DrdI GACnn_nn'nnGTC
Cuts at: 2082 2940 2082
Size: 858 3518
EaeI y'GGCC_r
Cuts at: 644 935 1004 1961 2135 2526 2553 3898 644
Size: 291 69 957 174 391 27 1345 1122
Fragments arranged by size:
1345 1122 957 391 291 174 69 27
EagI C'GGCC_G
Cuts at: 1961 1961
Size: 4376
EarI CTCTTCn'nnn_
Cuts at: 659 2399 2609 659
Size: 1740 210 2426
Fragments arranged by size:
2426 1740 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2584 2894 3040 4186 2584
Size: 310 146 1146 2774
Fragments arranged by size:
2774 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1347 1388 1347
Size: 41 4335
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 423 1160 2201 2633 3380 3648 271
Size: 152 737 1041 432 747 268 999
Fragments arranged by size:
1041 999 747 737 432 268 152
EcoO109I rG'GnC_Cy
Cuts at: 263 1515 263
Size: 1252 3124
EcoRI G'AATT_C
Cuts at: 342 1380 342
Size: 1038 3338
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2395 2395
Size: 4376
FspI TGC'GCA
Cuts at: 2157 2157
Size: 4376
HaeII r_GCGC'y
Cuts at: 463 576 866 921 1269 1810 2058 3080 4059
Size: 113 290 55 348 541 248 1022 979
Cuts at: 4059 463
Size: 780
Fragments arranged by size:
1022 979 780 541 348 290 248 113
55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 341 373 540 572 2218 2248 2250 2280 341
Size: 32 167 32 1646 30 2 30 2437
Fragments arranged by size:
2437 1646 167 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1415 1415
Size: 4376
KasI G'GCGC_C
Cuts at: 459 1806 2054 459
Size: 1347 248 2781
Fragments arranged by size:
2781 1347 248
KpnI G_GTAC'C
Cuts at: 318 1396 318
Size: 1078 3298
MluI A'CGCG_T
Cuts at: 3809 3809
Size: 4376
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1116 1334 3047 3231 1116
Size: 218 1713 184 2261
Fragments arranged by size:
2261 1713 218 184
MscI TGG'CCA
Cuts at: 2137 2137
Size: 4376
MslI CAynn'nnrTG
Cuts at: 323 2365 2492 323
Size: 2042 127 2207
Fragments arranged by size:
2207 2042 127
MspA1I CmG'CkG
Cuts at: 827 1487 1801 2161 3174 3419 827
Size: 660 314 360 1013 245 1784
Fragments arranged by size:
1784 1013 660 360 314 245
NaeI GCC'GGC
Cuts at: 648 2557 648
Size: 1909 2467
NarI GG'CG_CC
Cuts at: 460 1807 2055 460
Size: 1347 248 2781
Fragments arranged by size:
2781 1347 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4376
NdeI CA'TA_TG
Cuts at: 435 435
Size: 4376
NgoMIV G'CCGG_C
Cuts at: 646 2555 646
Size: 1909 2467
NspI r_CATG'y
Cuts at: 326 1429 1929 2836 326
Size: 1103 500 907 1866
Fragments arranged by size:
1866 1103 907 500
PciI A'CATG_T
Cuts at: 322 2832 322
Size: 2510 1866
PflMI CCAn_nnn'nTGG
Cuts at: 223 223
Size: 4376
PmeI GTTT'AAAC
Cuts at: 1375 1375
Size: 4376
PmlI CAC'GTG
Cuts at: 179 706 179
Size: 527 3849
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3515 3547 3515
Size: 32 4344
PstI C_TGCA'G
Cuts at: 1423 2108 1423
Size: 685 3691
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4376
PvuII CAG'CTG
Cuts at: 1801 2161 1801
Size: 360 4016
RsrII CG'GwC_CG
Cuts at: 2571 2571
Size: 4376
SacI G_AGCT'C
Cuts at: 1349 1390 1349
Size: 41 4335
SalI G'TCGA_C
Cuts at: 1413 1413
Size: 4376
SapI GCTCTTCn'nnn_
Cuts at: 2399 2609 2399
Size: 210 4166
SbfI CC_TGCA'GG
Cuts at: 1423 1423
Size: 4376
ScaI AGT'ACT
Cuts at: 3893 3893
Size: 4376
SfcI C'TryA_G
Cuts at: 33 794 1270 1419 2104 3097 3288 33
Size: 761 476 149 685 993 191 1121
Fragments arranged by size:
1121 993 761 685 476 191 149
SfoI GGC'GCC
Cuts at: 461 1808 2056 461
Size: 1347 248 2781
Fragments arranged by size:
2781 1347 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4376
SmaI CCC'GGG
Cuts at: 1398 1398
Size: 4376
SmlI C'TyrA_G
Cuts at: 1531 2446 2938 3200 1531
Size: 915 492 262 2707
Fragments arranged by size:
2707 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4376
SphI G_CATG'C
Cuts at: 1429 1929 1429
Size: 500 3876
StuI AGG'CCT
Cuts at: 614 614
Size: 4376
StyI C'CwwG_G
Cuts at: 107 1081 1245 1510 1864 107
Size: 974 164 265 354 2619
Fragments arranged by size:
2619 974 354 265 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 342 630 1959 2092 2551 2760 342
Size: 288 1329 133 459 209 1958
Fragments arranged by size:
1958 1329 459 288 209 133
TatI w'GTAC_w
Cuts at: 3891 3891
Size: 4376
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1224 1256 3893 3925 1224
Size: 32 2637 32 1675
Fragments arranged by size:
2637 1675 32 32
Tth111I GACn'n_nGTC
Cuts at: 2173 2173
Size: 4376
XbaI T'CTAG_A
Cuts at: 1407 1407
Size: 4376
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 751 1871 751
Size: 1120 3256
XmaI C'CCGG_G
Cuts at: 1396 1396
Size: 4376
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PflMI PmeI PmlI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NheI NotI NruI NsiI
PacI PasI PfoI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI