Restriction map for
pF1KB8437
(Circular) (Six-base) MAPSORT of: pF1KB8437.seq Check: 9084 from: 1 to: 4691
pF1KB8437 4691 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1729 1729
Size: 4691
Acc65I G'GTAC_C
Cuts at: 1707 1707
Size: 4691
AclI AA'CG_TT
Cuts at: 4531 4531
Size: 4691
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 1150 1332 2516 2948 3301 3963 279
Size: 871 182 1184 432 353 662 1007
Fragments arranged by size:
1184 1007 871 662 432 353 182
AfeI AGC'GCT
Cuts at: 292 313 292
Size: 21 4670
AflIII A'CryG_T
Cuts at: 693 2043 3843 4124 693
Size: 1350 1800 281 1260
Fragments arranged by size:
1800 1350 1260 281
AhdI GACnn_n'nnGTC
Cuts at: 1581 1581
Size: 4691
AlwNI CAG_nnn'CTG
Cuts at: 1996 3434 1996
Size: 1438 3253
ApaLI G'TGCA_C
Cuts at: 3529 3529
Size: 4691
ApoI r'AATT_y
Cuts at: 602 867 1695 4044 4300 602
Size: 265 828 2349 256 993
Fragments arranged by size:
2349 993 828 265 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4691
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4691
AvaI C'yCGr_G
Cuts at: 236 387 1103 1190 1711 236
Size: 151 716 87 521 3216
Fragments arranged by size:
3216 716 521 151 87
BamHI G'GATC_C
Cuts at: 1369 1716 1369
Size: 347 4344
BanI G'GyrC_C
Cuts at: 1707 2121 2369 2404 1707
Size: 414 248 35 3994
Fragments arranged by size:
3994 414 248 35
BanII G_rGCy'C
Cuts at: 647 1705 2735 647
Size: 1058 1030 2603
Fragments arranged by size:
2603 1058 1030
BbeI G_GCGC'C
Cuts at: 2125 2373 2125
Size: 248 4443
BbsI GAAGACnn'nnnn_
Cuts at: 518 530 1274 518
Size: 12 744 3935
Fragments arranged by size:
3935 744 12
BbvCI CC'TCA_GC
Cuts at: 200 730 200
Size: 530 4161
BciVI GTATCCnnnnn_n'
Cuts at: 2589 3645 4691 2589
Size: 1056 1046 2589
Fragments arranged by size:
2589 1056 1046
BclI T'GATC_A
Cuts at: 1184 2211 1184
Size: 1027 3664
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4691
BglII A'GATC_T
Cuts at: 858 2206 4243 858
Size: 1348 2037 1306
Fragments arranged by size:
2037 1348 1306
BlpI GC'TnA_GC
Cuts at: 1803 1803
Size: 4691
Bme1580I G_kGCm'C
Cuts at: 2316 2409 3533 2316
Size: 93 1124 3474
Fragments arranged by size:
3474 1124 93
BmgBI CAC'GTC
Cuts at: 245 245
Size: 4691
BmrI ACTGGGnnnn_n'
Cuts at: 1903 1984 2319 4463 1903
Size: 81 335 2144 2131
Fragments arranged by size:
2144 2131 335 81
BmtI G_CTAG'C
Cuts at: 253 253
Size: 4691
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3058 3058
Size: 4691
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 1258 184
Size: 1074 3617
Bpu10I CC'TnA_GC
Cuts at: 200 730 200
Size: 530 4161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 948 1576 1867 2746 3460 3758 948
Size: 628 291 879 714 298 1881
Fragments arranged by size:
1881 879 714 628 298 291
BsaI GGTCTCn'nnnn_
Cuts at: 418 4395 418
Size: 3977 714
BsaAI yAC'GTr
Cuts at: 2044 2044
Size: 4691
BsaBI GATnn'nnATC
Cuts at: 6 2205 4242 6
Size: 2199 2037 455
Fragments arranged by size:
2199 2037 455
BsaHI Gr'CG_yC
Cuts at: 2122 2370 3072 4568 2122
Size: 248 702 1496 2245
Fragments arranged by size:
2245 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1880 2401 3079 3490 3637 3911 1880
Size: 521 678 411 147 274 2660
Fragments arranged by size:
2660 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 217 217
Size: 4691
BseYI C'CCAG_C
Cuts at: 1293 1480 2116 2874 3539 3947 1293
Size: 187 636 758 665 408 2037
Fragments arranged by size:
2037 758 665 636 408 187
BsiEI CG_ry'CG
Cuts at: 104 2233 2279 3509 104
Size: 2129 46 1230 1286
Fragments arranged by size:
2129 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 394 647 1705 2483 2673 3533 394
Size: 253 1058 778 190 860 1552
Fragments arranged by size:
1552 1058 860 778 253 190
BsmI GAATG_Cn'
Cuts at: 1039 1315 1039
Size: 276 4415
Bsp1286I G_dGCh'C
Cuts at: 394 647 1705 2316 2409 2483 2673 2735 3533
Size: 253 1058 611 93 74 190 62 798
Cuts at: 3533 394
Size: 1552
Fragments arranged by size:
1552 1058 798 611 253 190 93 74
62
BspHI T'CATG_A
Cuts at: 552 3123 552
Size: 2571 2120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1741 1955 2257 2638 1741
Size: 214 302 381 3794
Fragments arranged by size:
3794 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1053 2983 3037 4521 4686 50
Size: 1003 1930 54 1484 165 55
Fragments arranged by size:
1930 1484 1003 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2603 2603
Size: 4691
BsrFI r'CCGG_y
Cuts at: 2689 2870 2689
Size: 181 4510
BssSI C'ACGA_G
Cuts at: 709 1602 2962 3670 709
Size: 893 1360 708 1730
Fragments arranged by size:
1730 1360 893 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2068 2068
Size: 4691
BstBI TT'CG_AA
Cuts at: 3052 3052
Size: 4691
BstXI CCAn_nnnn'nTGG
Cuts at: 711 711
Size: 4691
BstYI r'GATC_y
Cuts at: 1 858 1369 1716 2183 2206 2541 2787 3093
Size: 857 511 347 467 23 335 246 306
Cuts at: 3093 3105 3191 4243 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 857 511 467 449 347 335 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4691
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 840 2641 2822 2985 840
Size: 1801 181 163 2546
Fragments arranged by size:
2546 1801 181 163
BtsI GCAGTG_nn'
Cuts at: 336 1429 1934 336
Size: 1093 505 3093
Fragments arranged by size:
3093 1093 505
DraI TTT'AAA
Cuts at: 1690 1690
Size: 4691
DrdI GACnn_nn'nnGTC
Cuts at: 2397 3741 2397
Size: 1344 3347
EaeI y'GGCC_r
Cuts at: 113 176 188 2276 2450 2841 2868 4213 113
Size: 63 12 2088 174 391 27 1345 591
Fragments arranged by size:
2088 1345 591 391 174 63 27 12
EagI C'GGCC_G
Cuts at: 2276 2276
Size: 4691
EarI CTCTTCn'nnn_
Cuts at: 635 1653 2714 2924 635
Size: 1018 1061 210 2402
Fragments arranged by size:
2402 1061 1018 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2899 3641 3787 4501 2899
Size: 742 146 714 3089
Fragments arranged by size:
3089 742 714 146
EcoICRI GAG'CTC
Cuts at: 645 1703 645
Size: 1058 3633
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 279 1150 1258 1332 2516 2948 3301 3963
Size: 95 871 108 74 1184 432 353 662
Cuts at: 3963 184
Size: 912
Fragments arranged by size:
1184 912 871 662 432 353 108 95
74
EcoO109I rG'GnC_Cy
Cuts at: 1830 1830
Size: 4691
EcoRI G'AATT_C
Cuts at: 1695 1695
Size: 4691
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 664 1072 1195 2710 664
Size: 408 123 1515 2645
Fragments arranged by size:
2645 1515 408 123
FspI TGC'GCA
Cuts at: 2472 2472
Size: 4691
HaeII r_GCGC'y
Cuts at: 294 315 2125 2373 3603 4374 294
Size: 21 1810 248 1230 771 611
Fragments arranged by size:
1810 1230 771 611 248 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1567 1599 2533 2563 2565 2595 1567
Size: 32 934 30 2 30 3663
Fragments arranged by size:
3663 934 32 30 30 2
HincII GTy'rAC
Cuts at: 1730 1730
Size: 4691
KasI G'GCGC_C
Cuts at: 2121 2369 2121
Size: 248 4443
KpnI G_GTAC'C
Cuts at: 1711 1711
Size: 4691
MluI A'CGCG_T
Cuts at: 4124 4124
Size: 4691
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3450 3634 3450
Size: 184 4507
MscI TGG'CCA
Cuts at: 115 2452 115
Size: 2337 2354
MslI CAynn'nnrTG
Cuts at: 709 2680 2807 709
Size: 1971 127 2593
Fragments arranged by size:
2593 1971 127
MspA1I CmG'CkG
Cuts at: 182 1227 1802 2116 2476 3260 3505 182
Size: 1045 575 314 360 784 245 1368
Fragments arranged by size:
1368 1045 784 575 360 314 245
NaeI GCC'GGC
Cuts at: 2872 2872
Size: 4691
NarI GG'CG_CC
Cuts at: 2122 2370 2122
Size: 248 4443
NcoI C'CATG_G
Cuts at: 107 107
Size: 4691
NgoMIV G'CCGG_C
Cuts at: 2870 2870
Size: 4691
NheI G'CTAG_C
Cuts at: 249 249
Size: 4691
NspI r_CATG'y
Cuts at: 697 1159 1744 2244 3847 697
Size: 462 585 500 1603 1541
Fragments arranged by size:
1603 1541 585 500 462
PciI A'CATG_T
Cuts at: 693 3843 693
Size: 3150 1541
PfoI T'CCnGG_A
Cuts at: 809 1274 809
Size: 465 4226
PmeI GTTT'AAAC
Cuts at: 1690 1690
Size: 4691
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1489 1521 3137 3169 1489
Size: 32 1616 32 3011
Fragments arranged by size:
3011 1616 32 32
PshAI GACnn'nnGTC
Cuts at: 366 366
Size: 4691
PsiI TTA'TAA
Cuts at: 1046 1046
Size: 4691
PspXI vC'TCGA_Gb
Cuts at: 387 1190 387
Size: 803 3888
PstI C_TGCA'G
Cuts at: 160 311 754 1738 2423 160
Size: 151 443 984 685 2428
Fragments arranged by size:
2428 984 685 443 151
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4691
PvuII CAG'CTG
Cuts at: 1227 2116 2476 1227
Size: 889 360 3442
Fragments arranged by size:
3442 889 360
RsrII CG'GwC_CG
Cuts at: 184 2886 184
Size: 2702 1989
SacI G_AGCT'C
Cuts at: 647 1705 647
Size: 1058 3633
SalI G'TCGA_C
Cuts at: 1728 1728
Size: 4691
SapI GCTCTTCn'nnn_
Cuts at: 635 2714 2924 635
Size: 2079 210 2402
Fragments arranged by size:
2402 2079 210
SbfI CC_TGCA'GG
Cuts at: 1738 1738
Size: 4691
ScaI AGT'ACT
Cuts at: 4208 4208
Size: 4691
SfcI C'TryA_G
Cuts at: 33 156 307 750 820 1537 1590 1734 2419
Size: 123 151 443 70 717 53 144 685
Cuts at: 2419 3387 3578 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 717 685 443 191 151 144
123 70 53
SfoI GGC'GCC
Cuts at: 2123 2371 2123
Size: 248 4443
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4691
SmaI CCC'GGG
Cuts at: 1713 1713
Size: 4691
SmlI C'TyrA_G
Cuts at: 387 927 1103 1190 1555 1846 2761 3475 3737
Size: 540 176 87 365 291 915 714 262
Cuts at: 3737 387
Size: 1341
Fragments arranged by size:
1341 915 714 540 365 291 262 176
87
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4691
SphI G_CATG'C
Cuts at: 1744 2244 1744
Size: 500 4191
StuI AGG'CCT
Cuts at: 547 547
Size: 4691
StyI C'CwwG_G
Cuts at: 107 116 191 461 501 1825 2179 107
Size: 9 75 270 40 1324 354 2619
Fragments arranged by size:
2619 1324 354 270 75 40 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1216 1285 2274 2407 2866 3075 1216
Size: 69 989 133 459 209 2832
Fragments arranged by size:
2832 989 459 209 133 69
TatI w'GTAC_w
Cuts at: 4206 4206
Size: 4691
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4208 4240 4208
Size: 32 4659
Tth111I GACn'n_nGTC
Cuts at: 2488 2488
Size: 4691
XbaI T'CTAG_A
Cuts at: 1722 1722
Size: 4691
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 777 2186 777
Size: 1409 3282
XhoI C'TCGA_G
Cuts at: 387 1103 1190 387
Size: 716 87 3888
Fragments arranged by size:
3888 716 87
XmaI C'CCGG_G
Cuts at: 1711 1711
Size: 4691
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PfoI PmeI PpiI
PshAI PsiI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BsaXI BsgI BsiWI
BsmBI BspEI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NotI NruI NsiI PacI PasI PflMI
PmlI PpuMI PspOMI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XmnI ZraI