Restriction map for
pF1KB8436
(Circular) (Six-base) MAPSORT of: pF1KB8436.seq Check: 3360 from: 1 to: 4190
pF1KB8436 4190 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 974 1228 974
Size: 254 3936
Acc65I G'GTAC_C
Cuts at: 1206 1206
Size: 4190
AclI AA'CG_TT
Cuts at: 4030 4030
Size: 4190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1057 1186 2015 2447 2800 3462 1057
Size: 129 829 432 353 662 1785
Fragments arranged by size:
1785 829 662 432 353 129
AfeI AGC'GCT
Cuts at: 800 800
Size: 4190
AflIII A'CryG_T
Cuts at: 1542 3342 3623 1542
Size: 1800 281 2109
Fragments arranged by size:
2109 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1495 2933 1495
Size: 1438 2752
ApaLI G'TGCA_C
Cuts at: 3028 3028
Size: 4190
ApoI r'AATT_y
Cuts at: 1027 1194 3543 3799 1027
Size: 167 2349 256 1418
Fragments arranged by size:
2349 1418 256 167
AseI AT'TA_AT
Cuts at: 20 20
Size: 4190
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
AvaI C'yCGr_G
Cuts at: 241 1022 1210 241
Size: 781 188 3221
Fragments arranged by size:
3221 781 188
BamHI G'GATC_C
Cuts at: 1215 1215
Size: 4190
BanI G'GyrC_C
Cuts at: 1206 1620 1868 1903 1206
Size: 414 248 35 3493
Fragments arranged by size:
3493 414 248 35
BanII G_rGCy'C
Cuts at: 227 1204 2234 227
Size: 977 1030 2183
Fragments arranged by size:
2183 1030 977
BbeI G_GCGC'C
Cuts at: 1624 1872 1624
Size: 248 3942
BciVI GTATCCnnnnn_n'
Cuts at: 298 749 2088 3144 4190 298
Size: 451 1339 1056 1046 298
Fragments arranged by size:
1339 1056 1046 451 298
BclI T'GATC_A
Cuts at: 377 813 1710 377
Size: 436 897 2857
Fragments arranged by size:
2857 897 436
BglI GCCn_nnn'nGGC
Cuts at: 370 370
Size: 4190
BglII A'GATC_T
Cuts at: 936 1705 3742 936
Size: 769 2037 1384
Fragments arranged by size:
2037 1384 769
BlpI GC'TnA_GC
Cuts at: 1302 1302
Size: 4190
Bme1580I G_kGCm'C
Cuts at: 1815 1908 3032 1815
Size: 93 1124 2973
Fragments arranged by size:
2973 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1402 1483 1818 3962 1402
Size: 81 335 2144 1630
Fragments arranged by size:
2144 1630 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2557 2557
Size: 4190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 1138 136
Size: 1002 3188
Bpu10I CC'TnA_GC
Cuts at: 139 139
Size: 4190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 382 1366 2245 2959 3257 211
Size: 171 984 879 714 298 1144
Fragments arranged by size:
1144 984 879 714 298 171
BsaI GGTCTCn'nnnn_
Cuts at: 3894 3894
Size: 4190
BsaAI yAC'GTr
Cuts at: 1094 1543 1094
Size: 449 3741
BsaBI GATnn'nnATC
Cuts at: 6 286 1704 3741 6
Size: 280 1418 2037 455
Fragments arranged by size:
2037 1418 455 280
BsaHI Gr'CG_yC
Cuts at: 212 1621 1869 2571 4067 212
Size: 1409 248 702 1496 335
Fragments arranged by size:
1496 1409 702 335 248
BsaWI w'CCGG_w
Cuts at: 1379 1900 2578 2989 3136 3410 1379
Size: 521 678 411 147 274 2159
Fragments arranged by size:
2159 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 454 484 454
Size: 30 4160
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 897 961 160
Size: 737 64 3389
Fragments arranged by size:
3389 737 64
BseYI C'CCAG_C
Cuts at: 1615 2373 3038 3446 1615
Size: 758 665 408 2359
Fragments arranged by size:
2359 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1732 1778 3008 104
Size: 1628 46 1230 1286
Fragments arranged by size:
1628 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 227 422 1204 1982 2172 3032 227
Size: 195 782 778 190 860 1385
Fragments arranged by size:
1385 860 782 778 195 190
BsmI GAATG_Cn'
Cuts at: 541 541
Size: 4190
Bsp1286I G_dGCh'C
Cuts at: 227 422 1204 1815 1908 1982 2172 2234 3032
Size: 195 782 611 93 74 190 62 798
Cuts at: 3032 227
Size: 1385
Fragments arranged by size:
1385 798 782 611 195 190 93 74
62
BspHI T'CATG_A
Cuts at: 2622 2622
Size: 4190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 632 1240 1454 1756 2137 632
Size: 608 214 302 381 2685
Fragments arranged by size:
2685 608 381 302 214
BsrBI CCG'CTC
Cuts at: 50 186 2482 2536 4020 4185 50
Size: 136 2296 54 1484 165 55
Fragments arranged by size:
2296 1484 165 136 55 54
BsrDI GCAATG_nn'
Cuts at: 2102 2102
Size: 4190
BsrFI r'CCGG_y
Cuts at: 2188 2369 2188
Size: 181 4009
BssSI C'ACGA_G
Cuts at: 823 2461 3169 823
Size: 1638 708 1844
Fragments arranged by size:
1844 1638 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1567 1567
Size: 4190
BstBI TT'CG_AA
Cuts at: 2551 2551
Size: 4190
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4190
BstYI r'GATC_y
Cuts at: 1 401 936 1215 1682 1705 2040 2286 2592
Size: 400 535 279 467 23 335 246 306
Cuts at: 2592 2604 2690 3742 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 535 467 449 400 335 306 279
246 86 23 12
BtgI C'CryG_G
Cuts at: 294 386 294
Size: 92 4098
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2140 2321 2484 2140
Size: 181 163 3846
Fragments arranged by size:
3846 181 163
BtsI GCAGTG_nn'
Cuts at: 759 1433 759
Size: 674 3516
DraI TTT'AAA
Cuts at: 1189 1189
Size: 4190
DrdI GACnn_nn'nnGTC
Cuts at: 117 1896 3240 117
Size: 1779 1344 1067
Fragments arranged by size:
1779 1344 1067
EaeI y'GGCC_r
Cuts at: 362 1775 1949 2340 2367 3712 362
Size: 1413 174 391 27 1345 840
Fragments arranged by size:
1413 1345 840 391 174 27
EagI C'GGCC_G
Cuts at: 1775 1775
Size: 4190
EarI CTCTTCn'nnn_
Cuts at: 2213 2423 2213
Size: 210 3980
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 180 2398 3140 3286 4000 180
Size: 2218 742 146 714 370
Fragments arranged by size:
2218 742 714 370 146
EcoICRI GAG'CTC
Cuts at: 225 1202 225
Size: 977 3213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 1057 1138 1186 2015 2447 2800 3462 136
Size: 921 81 48 829 432 353 662 864
Fragments arranged by size:
921 864 829 662 432 353 81 48
EcoNI CCTnn'n_nnAGG
Cuts at: 142 142
Size: 4190
EcoO109I rG'GnC_Cy
Cuts at: 1329 1329
Size: 4190
EcoRI G'AATT_C
Cuts at: 1027 1194 1027
Size: 167 4023
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 661 885 2209 661
Size: 224 1324 2642
Fragments arranged by size:
2642 1324 224
FspI TGC'GCA
Cuts at: 1971 1971
Size: 4190
HaeII r_GCGC'y
Cuts at: 802 1624 1872 3102 3873 802
Size: 822 248 1230 771 1119
Fragments arranged by size:
1230 1119 822 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 454 486 641 673 2032 2062 2064 2094 454
Size: 32 155 32 1359 30 2 30 2550
Fragments arranged by size:
2550 1359 155 32 32 30 30 2
HincII GTy'rAC
Cuts at: 975 1229 975
Size: 254 3936
KasI G'GCGC_C
Cuts at: 1620 1868 1620
Size: 248 3942
KpnI G_GTAC'C
Cuts at: 1210 1210
Size: 4190
MluI A'CGCG_T
Cuts at: 3623 3623
Size: 4190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2949 3133 2949
Size: 184 4006
MscI TGG'CCA
Cuts at: 1951 1951
Size: 4190
MslI CAynn'nnrTG
Cuts at: 364 2179 2306 364
Size: 1815 127 2248
Fragments arranged by size:
2248 1815 127
MspA1I CmG'CkG
Cuts at: 1301 1615 1975 2759 3004 1301
Size: 314 360 784 245 2487
Fragments arranged by size:
2487 784 360 314 245
NaeI GCC'GGC
Cuts at: 2371 2371
Size: 4190
NarI GG'CG_CC
Cuts at: 1621 1869 1621
Size: 248 3942
NcoI C'CATG_G
Cuts at: 294 386 294
Size: 92 4098
NdeI CA'TA_TG
Cuts at: 489 489
Size: 4190
NgoMIV G'CCGG_C
Cuts at: 2369 2369
Size: 4190
NspI r_CATG'y
Cuts at: 1243 1743 3346 1243
Size: 500 1603 2087
Fragments arranged by size:
2087 1603 500
PciI A'CATG_T
Cuts at: 3342 3342
Size: 4190
PfoI T'CCnGG_A
Cuts at: 522 530 522
Size: 8 4182
PmeI GTTT'AAAC
Cuts at: 1189 1189
Size: 4190
PmlI CAC'GTG
Cuts at: 1094 1094
Size: 4190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2636 2668 2636
Size: 32 4158
PstI C_TGCA'G
Cuts at: 1237 1922 1237
Size: 685 3505
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4190
PvuII CAG'CTG
Cuts at: 1615 1975 1615
Size: 360 3830
RsrII CG'GwC_CG
Cuts at: 2385 2385
Size: 4190
SacI G_AGCT'C
Cuts at: 227 1204 227
Size: 977 3213
SalI G'TCGA_C
Cuts at: 973 1227 973
Size: 254 3936
SapI GCTCTTCn'nnn_
Cuts at: 2213 2423 2213
Size: 210 3980
SbfI CC_TGCA'GG
Cuts at: 1237 1237
Size: 4190
ScaI AGT'ACT
Cuts at: 3707 3707
Size: 4190
SfcI C'TryA_G
Cuts at: 33 1233 1918 2886 3077 33
Size: 1200 685 968 191 1146
Fragments arranged by size:
1200 1146 968 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 370 370
Size: 4190
SfoI GGC'GCC
Cuts at: 1622 1870 1622
Size: 248 3942
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
SmaI CCC'GGG
Cuts at: 1212 1212
Size: 4190
SmlI C'TyrA_G
Cuts at: 226 397 1345 2260 2974 3236 226
Size: 171 948 915 714 262 1180
Fragments arranged by size:
1180 948 915 714 262 171
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4190
SphI G_CATG'C
Cuts at: 1243 1743 1243
Size: 500 3690
StyI C'CwwG_G
Cuts at: 152 294 386 1324 1678 152
Size: 142 92 938 354 2664
Fragments arranged by size:
2664 938 354 142 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1773 1906 2365 2574 1773
Size: 133 459 209 3389
Fragments arranged by size:
3389 459 209 133
TatI w'GTAC_w
Cuts at: 1172 3705 1172
Size: 2533 1657
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 386 418 3707 3739 386
Size: 32 3289 32 837
Fragments arranged by size:
3289 837 32 32
Tth111I GACn'n_nGTC
Cuts at: 1987 1987
Size: 4190
XbaI T'CTAG_A
Cuts at: 942 1221 942
Size: 279 3911
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1685 1685
Size: 4190
XmaI C'CCGG_G
Cuts at: 1210 1210
Size: 4190
XmnI GAAnn'nnTTC
Cuts at: 355 1031 1042 355
Size: 676 11 3503
Fragments arranged by size:
3503 676 11
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PfoI PmeI PmlI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI BsgI BsiWI BsmBI BspEI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NheI NotI NruI NsiI
PacI PasI PflMI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI