Restriction map for
pF1KB8435
(Circular) (Six-base) MAPSORT of: pF1KB8435.seq Check: 3170 from: 1 to: 4622
pF1KB8435 4622 bp
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AccI GT'mk_AC
Cuts at: 1660 1660
Size: 4622
Acc65I G'GTAC_C
Cuts at: 1638 1638
Size: 4622
AclI AA'CG_TT
Cuts at: 4462 4462
Size: 4622
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 857 928 1349 2447 2879 3232 3894 331
Size: 526 71 421 1098 432 353 662 1059
Fragments arranged by size:
1098 1059 662 526 432 421 353 71
AflIII A'CryG_T
Cuts at: 1434 1974 3774 4055 1434
Size: 540 1800 281 2001
Fragments arranged by size:
2001 1800 540 281
AhdI GACnn_n'nnGTC
Cuts at: 199 807 199
Size: 608 4014
AleI CACnn'nnGTG
Cuts at: 118 118
Size: 4622
AlwNI CAG_nnn'CTG
Cuts at: 279 452 468 864 1927 3365 279
Size: 173 16 396 1063 1438 1536
Fragments arranged by size:
1536 1438 1063 396 173 16
ApaLI G'TGCA_C
Cuts at: 1438 1576 3460 1438
Size: 138 1884 2600
Fragments arranged by size:
2600 1884 138
ApoI r'AATT_y
Cuts at: 1044 1626 3975 4231 1044
Size: 582 2349 256 1435
Fragments arranged by size:
2349 1435 582 256
AscI GG'CGCG_CC
Cuts at: 1595 1595
Size: 4622
AseI AT'TA_AT
Cuts at: 20 20
Size: 4622
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4622
AvaI C'yCGr_G
Cuts at: 1590 1642 1590
Size: 52 4570
BamHI G'GATC_C
Cuts at: 1647 1647
Size: 4622
BanI G'GyrC_C
Cuts at: 793 1547 1638 2052 2300 2335 793
Size: 754 91 414 248 35 3080
Fragments arranged by size:
3080 754 414 248 91 35
BanII G_rGCy'C
Cuts at: 401 1636 2666 401
Size: 1235 1030 2357
Fragments arranged by size:
2357 1235 1030
BbeI G_GCGC'C
Cuts at: 2056 2304 2056
Size: 248 4374
BbsI GAAGACnn'nnnn_
Cuts at: 325 366 413 1163 1211 325
Size: 41 47 750 48 3736
Fragments arranged by size:
3736 750 48 47 41
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 424 458 424
Size: 34 4588
BciVI GTATCCnnnnn_n'
Cuts at: 466 2520 3576 4622 466
Size: 2054 1056 1046 466
Fragments arranged by size:
2054 1056 1046 466
BclI T'GATC_A
Cuts at: 2142 2142
Size: 4622
BglI GCCn_nnn'nGGC
Cuts at: 901 1559 901
Size: 658 3964
BglII A'GATC_T
Cuts at: 2137 4174 2137
Size: 2037 2585
BlpI GC'TnA_GC
Cuts at: 1734 1734
Size: 4622
Bme1580I G_kGCm'C
Cuts at: 1442 1580 2247 2340 3464 1442
Size: 138 667 93 1124 2600
Fragments arranged by size:
2600 1124 667 138 93
BmgBI CAC'GTC
Cuts at: 1443 1443
Size: 4622
BmrI ACTGGGnnnn_n'
Cuts at: 1345 1834 1915 2250 4394 1345
Size: 489 81 335 2144 1573
Fragments arranged by size:
2144 1573 489 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 308 1036 2989 308
Size: 728 1953 1941
Fragments arranged by size:
1953 1941 728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 490 1186 301
Size: 189 696 3737
Fragments arranged by size:
3737 696 189
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 991 1798 2677 3391 3689 502
Size: 489 807 879 714 298 1435
Fragments arranged by size:
1435 879 807 714 489 298
BsaI GGTCTCn'nnnn_
Cuts at: 506 1528 4326 506
Size: 1022 2798 802
Fragments arranged by size:
2798 1022 802
BsaAI yAC'GTr
Cuts at: 1437 1975 1437
Size: 538 4084
BsaBI GATnn'nnATC
Cuts at: 6 2136 4173 6
Size: 2130 2037 455
Fragments arranged by size:
2130 2037 455
BsaHI Gr'CG_yC
Cuts at: 2053 2301 3003 4499 2053
Size: 248 702 1496 2176
Fragments arranged by size:
2176 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1811 2332 3010 3421 3568 3842 1811
Size: 521 678 411 147 274 2591
Fragments arranged by size:
2591 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 331 361 816 846 1137 1167 331
Size: 30 455 30 291 30 3786
Fragments arranged by size:
3786 455 291 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 367 1546 367
Size: 1179 3443
BseYI C'CCAG_C
Cuts at: 2047 2805 3470 3878 2047
Size: 758 665 408 2791
Fragments arranged by size:
2791 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 111 195 619 1142 111
Size: 84 424 523 3591
Fragments arranged by size:
3591 523 424 84
BsiEI CG_ry'CG
Cuts at: 104 2164 2210 3440 104
Size: 2060 46 1230 1286
Fragments arranged by size:
2060 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 401 1442 1580 1636 2414 2604 3464 401
Size: 1041 138 56 778 190 860 1559
Fragments arranged by size:
1559 1041 860 778 190 138 56
BsmI GAATG_Cn'
Cuts at: 315 315
Size: 4622
Bsp1286I G_dGCh'C
Cuts at: 401 1442 1580 1636 2247 2340 2414 2604 2666
Size: 1041 138 56 611 93 74 190 62
Cuts at: 2666 3464 401
Size: 798 1559
Fragments arranged by size:
1559 1041 798 611 190 138 93 74
62 56
BspHI T'CATG_A
Cuts at: 3054 3054
Size: 4622
BspMI ACCTGCnnnn'nnnn_
Cuts at: 133 539 1394 1605 1672 1886 2188 2569 133
Size: 406 855 211 67 214 302 381 2186
Fragments arranged by size:
2186 855 406 381 302 214 211 67
BsrBI CCG'CTC
Cuts at: 50 2914 2968 4452 4617 50
Size: 2864 54 1484 165 55
Fragments arranged by size:
2864 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2534 2534
Size: 4622
BsrFI r'CCGG_y
Cuts at: 214 1508 2620 2801 214
Size: 1294 1112 181 2035
Fragments arranged by size:
2035 1294 1112 181
BsrGI T'GTAC_A
Cuts at: 719 719
Size: 4622
BssHII G'CGCG_C
Cuts at: 1595 1595
Size: 4622
BssSI C'ACGA_G
Cuts at: 730 2893 3601 730
Size: 2163 708 1751
Fragments arranged by size:
2163 1751 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1575 1999 1575
Size: 424 4198
BstBI TT'CG_AA
Cuts at: 2983 2983
Size: 4622
BstEII G'GTnAC_C
Cuts at: 120 120
Size: 4622
BstXI CCAn_nnnn'nTGG
Cuts at: 749 1032 749
Size: 283 4339
BstYI r'GATC_y
Cuts at: 1 296 464 1647 2114 2137 2472 2718 3024
Size: 295 168 1183 467 23 335 246 306
Cuts at: 3024 3036 3122 4174 1
Size: 12 86 1052 449
Fragments arranged by size:
1183 1052 467 449 335 306 295 246
168 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 656 656
Size: 4622
BtgI C'CryG_G
Cuts at: 107 116 916 935 1241 107
Size: 9 800 19 306 3488
Fragments arranged by size:
3488 800 306 19 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1452 2572 2753 2916 1452
Size: 1120 181 163 3158
Fragments arranged by size:
3158 1120 181 163
BtsI GCAGTG_nn'
Cuts at: 569 1584 1865 569
Size: 1015 281 3326
Fragments arranged by size:
3326 1015 281
DraI TTT'AAA
Cuts at: 1621 1621
Size: 4622
DrdI GACnn_nn'nnGTC
Cuts at: 2328 3672 2328
Size: 1344 3278
EaeI y'GGCC_r
Cuts at: 110 216 539 1034 1391 2207 2381 2772 2799
Size: 106 323 495 357 816 174 391 27
Cuts at: 2799 4144 110
Size: 1345 588
Fragments arranged by size:
1345 816 588 495 391 357 323 174
106 27
EagI C'GGCC_G
Cuts at: 2207 2207
Size: 4622
EarI CTCTTCn'nnn_
Cuts at: 2645 2855 2645
Size: 210 4412
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2830 3572 3718 4432 2830
Size: 742 146 714 3020
Fragments arranged by size:
3020 742 714 146
EcoICRI GAG'CTC
Cuts at: 399 1634 399
Size: 1235 3387
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 331 490 857 928 1186 1349 2447 2879
Size: 30 159 367 71 258 163 1098 432
Cuts at: 2879 3232 3894 301
Size: 353 662 1029
Fragments arranged by size:
1098 1029 662 432 367 353 258 163
159 71 30
EcoO109I rG'GnC_Cy
Cuts at: 660 903 1761 660
Size: 243 858 3521
Fragments arranged by size:
3521 858 243
EcoRI G'AATT_C
Cuts at: 1626 1626
Size: 4622
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1165 2641 1165
Size: 1476 3146
FseI GG_CCGG'CC
Cuts at: 1512 1512
Size: 4622
FspI TGC'GCA
Cuts at: 2403 2403
Size: 4622
HaeII r_GCGC'y
Cuts at: 2056 2304 3534 4305 2056
Size: 248 1230 771 2373
Fragments arranged by size:
2373 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 185 217 331 363 793 825 1137 1169 2464
Size: 32 114 32 430 32 312 32 1295
Cuts at: 2464 2494 2496 2526 185
Size: 30 2 30 2281
Fragments arranged by size:
2281 1295 430 312 114 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1266 1661 1266
Size: 395 4227
KasI G'GCGC_C
Cuts at: 2052 2300 2052
Size: 248 4374
KpnI G_GTAC'C
Cuts at: 1642 1642
Size: 4622
MluI A'CGCG_T
Cuts at: 4055 4055
Size: 4622
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 216 344 3381 3565 216
Size: 128 3037 184 1273
Fragments arranged by size:
3037 1273 184 128
MscI TGG'CCA
Cuts at: 112 541 1036 1393 2383 112
Size: 429 495 357 990 2351
Fragments arranged by size:
2351 990 495 429 357
MslI CAynn'nnrTG
Cuts at: 118 224 1027 2611 2738 118
Size: 106 803 1584 127 2002
Fragments arranged by size:
2002 1584 803 127 106
MspA1I CmG'CkG
Cuts at: 1611 1733 2047 2407 3191 3436 1611
Size: 122 314 360 784 245 2797
Fragments arranged by size:
2797 784 360 314 245 122
NaeI GCC'GGC
Cuts at: 1510 2803 1510
Size: 1293 3329
NarI GG'CG_CC
Cuts at: 2053 2301 2053
Size: 248 4374
NcoI C'CATG_G
Cuts at: 107 107
Size: 4622
NgoMIV G'CCGG_C
Cuts at: 1508 2801 1508
Size: 1293 3329
NspI r_CATG'y
Cuts at: 707 1675 2175 3778 707
Size: 968 500 1603 1551
Fragments arranged by size:
1603 1551 968 500
PasI CC'CwG_GG
Cuts at: 1306 1306
Size: 4622
PciI A'CATG_T
Cuts at: 3774 3774
Size: 4622
PflMI CCAn_nnn'nTGG
Cuts at: 279 369 279
Size: 90 4532
PfoI T'CCnGG_A
Cuts at: 467 467
Size: 4622
PmeI GTTT'AAAC
Cuts at: 1621 1621
Size: 4622
PmlI CAC'GTG
Cuts at: 1437 1437
Size: 4622
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3068 3100 3068
Size: 32 4590
PpuMI rG'GwC_Cy
Cuts at: 660 660
Size: 4622
PstI C_TGCA'G
Cuts at: 564 1616 1669 2354 564
Size: 1052 53 685 2832
Fragments arranged by size:
2832 1052 685 53
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4622
PvuII CAG'CTG
Cuts at: 2047 2407 2047
Size: 360 4262
RsrII CG'GwC_CG
Cuts at: 2817 2817
Size: 4622
SacI G_AGCT'C
Cuts at: 401 1636 401
Size: 1235 3387
SalI G'TCGA_C
Cuts at: 1659 1659
Size: 4622
SapI GCTCTTCn'nnn_
Cuts at: 2645 2855 2645
Size: 210 4412
SbfI CC_TGCA'GG
Cuts at: 1669 1669
Size: 4622
ScaI AGT'ACT
Cuts at: 427 4139 427
Size: 3712 910
SexAI A'CCwGG_T
Cuts at: 368 1343 368
Size: 975 3647
SfcI C'TryA_G
Cuts at: 33 429 560 1612 1665 2350 3318 3509 33
Size: 396 131 1052 53 685 968 191 1146
Fragments arranged by size:
1146 1052 968 685 396 191 131 53
SfoI GGC'GCC
Cuts at: 2054 2302 2054
Size: 248 4374
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4622
SmaI CCC'GGG
Cuts at: 1644 1644
Size: 4622
SmlI C'TyrA_G
Cuts at: 517 1006 1777 2692 3406 3668 517
Size: 489 771 915 714 262 1471
Fragments arranged by size:
1471 915 771 714 489 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4622
SphI G_CATG'C
Cuts at: 1675 2175 1675
Size: 500 4122
StuI AGG'CCT
Cuts at: 1285 1285
Size: 4622
StyI C'CwwG_G
Cuts at: 107 1756 2110 107
Size: 1649 354 2619
Fragments arranged by size:
2619 1649 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2205 2338 2797 3006 2205
Size: 133 459 209 3821
Fragments arranged by size:
3821 459 209 133
TatI w'GTAC_w
Cuts at: 425 719 1490 4137 425
Size: 294 771 2647 910
Fragments arranged by size:
2647 910 771 294
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 816 848 4139 4171 816
Size: 32 3291 32 1267
Fragments arranged by size:
3291 1267 32 32
Tth111I GACn'n_nGTC
Cuts at: 826 2419 826
Size: 1593 3029
XbaI T'CTAG_A
Cuts at: 1653 1653
Size: 4622
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2117 2117
Size: 4622
XmaI C'CCGG_G
Cuts at: 1642 1642
Size: 4622
XmnI GAAnn'nnTTC
Cuts at: 985 985
Size: 4622
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AlwNI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AlfI AloI ApaI
AvrII BaeI BbvCI BfrBI BmtI Bpu10I BsiWI BsmBI
BspEI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI