Restriction map for
pF1KB8413
(Circular) (Six-base) MAPSORT of: pF1KB8413.seq Check: 2774 from: 1 to: 5273
pF1KB8413 5273 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 2311 2311
Size: 5273
Acc65I G'GTAC_C
Cuts at: 2289 2289
Size: 5273
AclI AA'CG_TT
Cuts at: 5113 5113
Size: 5273
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 661 859 3098 3530 3883 4545 661
Size: 198 2239 432 353 662 1389
Fragments arranged by size:
2239 1389 662 432 353 198
AflIII A'CryG_T
Cuts at: 665 2114 2625 4425 4706 665
Size: 1449 511 1800 281 1232
Fragments arranged by size:
1800 1449 1232 511 281
AhdI GACnn_n'nnGTC
Cuts at: 1086 1671 1086
Size: 585 4688
AleI CACnn'nnGTG
Cuts at: 536 1850 536
Size: 1314 3959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 505 505
Size: 5273
AlwNI CAG_nnn'CTG
Cuts at: 726 1090 1743 2578 4016 726
Size: 364 653 835 1438 1983
Fragments arranged by size:
1983 1438 835 653 364
ApaLI G'TGCA_C
Cuts at: 4111 4111
Size: 5273
ApoI r'AATT_y
Cuts at: 1907 2277 4626 4882 1907
Size: 370 2349 256 2298
Fragments arranged by size:
2349 2298 370 256
AseI AT'TA_AT
Cuts at: 20 824 20
Size: 804 4469
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5273
AvaI C'yCGr_G
Cuts at: 353 1553 1711 2293 353
Size: 1200 158 582 3333
Fragments arranged by size:
3333 1200 582 158
BamHI G'GATC_C
Cuts at: 2298 2298
Size: 5273
BanI G'GyrC_C
Cuts at: 2289 2703 2951 2986 2289
Size: 414 248 35 4576
Fragments arranged by size:
4576 414 248 35
BanII G_rGCy'C
Cuts at: 578 1346 1451 2237 2287 3317 578
Size: 768 105 786 50 1030 2534
Fragments arranged by size:
2534 1030 786 768 105 50
BbeI G_GCGC'C
Cuts at: 2707 2955 2707
Size: 248 5025
BbsI GAAGACnn'nnnn_
Cuts at: 1310 1310
Size: 5273
BbvCI CC'TCA_GC
Cuts at: 278 1693 278
Size: 1415 3858
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1534 1568 90
Size: 34 1410 34 3795
Fragments arranged by size:
3795 1410 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3171 4227 5273 3171
Size: 1056 1046 3171
Fragments arranged by size:
3171 1056 1046
BclI T'GATC_A
Cuts at: 1727 2793 1727
Size: 1066 4207
BfrBI ATG'CAT
Cuts at: 877 877
Size: 5273
BglI GCCn_nnn'nGGC
Cuts at: 1325 1325
Size: 5273
BglII A'GATC_T
Cuts at: 2788 4825 2788
Size: 2037 3236
BlpI GC'TnA_GC
Cuts at: 2155 2385 2155
Size: 230 5043
Bme1580I G_kGCm'C
Cuts at: 2100 2898 2991 4115 2100
Size: 798 93 1124 3258
Fragments arranged by size:
3258 1124 798 93
BmgBI CAC'GTC
Cuts at: 447 666 447
Size: 219 5054
BmrI ACTGGGnnnn_n'
Cuts at: 378 1949 2485 2566 2901 5045 378
Size: 1571 536 81 335 2144 606
Fragments arranged by size:
2144 1571 606 536 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3640 3640
Size: 5273
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 763 2209 718
Size: 45 1446 3782
Fragments arranged by size:
3782 1446 45
Bpu10I CC'TnA_GC
Cuts at: 278 1693 278
Size: 1415 3858
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1741 2449 3328 4042 4340 1741
Size: 708 879 714 298 2674
Fragments arranged by size:
2674 879 714 708 298
BsaI GGTCTCn'nnnn_
Cuts at: 394 789 2029 4977 394
Size: 395 1240 2948 690
Fragments arranged by size:
2948 1240 690 395
BsaAI yAC'GTr
Cuts at: 2626 2626
Size: 5273
BsaBI GATnn'nnATC
Cuts at: 6 2787 4824 6
Size: 2781 2037 455
Fragments arranged by size:
2781 2037 455
BsaHI Gr'CG_yC
Cuts at: 2704 2952 3654 5150 2704
Size: 248 702 1496 2827
Fragments arranged by size:
2827 1496 702 248
BsaWI w'CCGG_w
Cuts at: 896 1274 2462 2983 3661 4072 4219 4493 896
Size: 378 1188 521 678 411 147 274 1676
Fragments arranged by size:
1676 1188 678 521 411 378 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1191 1221 1191
Size: 30 5243
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1080 1199 1080
Size: 119 5154
BseYI C'CCAG_C
Cuts at: 164 1161 2236 2698 3456 4121 4529 164
Size: 997 1075 462 758 665 408 908
Fragments arranged by size:
1075 997 908 758 665 462 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 344 344
Size: 5273
BsiEI CG_ry'CG
Cuts at: 104 2815 2861 4091 104
Size: 2711 46 1230 1286
Fragments arranged by size:
2711 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 578 1346 1451 1977 2287 3065 3255 4115 578
Size: 768 105 526 310 778 190 860 1736
Fragments arranged by size:
1736 860 778 768 526 310 190 105
BsmI GAATG_Cn'
Cuts at: 550 1223 1321 550
Size: 673 98 4502
Fragments arranged by size:
4502 673 98
Bsp1286I G_dGCh'C
Cuts at: 578 1346 1451 1977 2100 2237 2287 2898 2991
Size: 768 105 526 123 137 50 611 93
Cuts at: 2991 3065 3255 3317 4115 578
Size: 74 190 62 798 1736
Fragments arranged by size:
1736 798 768 611 526 190 137 123
105 93 74 62 50
BspEI T'CCGG_A
Cuts at: 1274 1274
Size: 5273
BspHI T'CATG_A
Cuts at: 3705 3705
Size: 5273
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1362 2323 2537 2839 3220 1362
Size: 961 214 302 381 3415
Fragments arranged by size:
3415 961 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3565 3619 5103 5268 50
Size: 3515 54 1484 165 55
Fragments arranged by size:
3515 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 812 1107 1407 3185 812
Size: 295 300 1778 2900
Fragments arranged by size:
2900 1778 300 295
BsrFI r'CCGG_y
Cuts at: 1748 1817 3271 3452 1748
Size: 69 1454 181 3569
Fragments arranged by size:
3569 1454 181 69
BsrGI T'GTAC_A
Cuts at: 284 1139 284
Size: 855 4418
BssSI C'ACGA_G
Cuts at: 502 3544 4252 502
Size: 3042 708 1523
Fragments arranged by size:
3042 1523 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2650 2650
Size: 5273
BstBI TT'CG_AA
Cuts at: 3634 3634
Size: 5273
BstXI CCAn_nnnn'nTGG
Cuts at: 1581 1581
Size: 5273
BstYI r'GATC_y
Cuts at: 1 1703 1715 2298 2765 2788 3123 3369 3675
Size: 1702 12 583 467 23 335 246 306
Cuts at: 3675 3687 3773 4825 1
Size: 12 86 1052 449
Fragments arranged by size:
1702 1052 583 467 449 335 306 246
86 23 12 12
Bsu36I CC'TnA_GG
Cuts at: 848 848
Size: 5273
BtgI C'CryG_G
Cuts at: 585 656 755 1190 585
Size: 71 99 435 4668
Fragments arranged by size:
4668 435 99 71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2034 3223 3404 3567 2034
Size: 1189 181 163 3740
Fragments arranged by size:
3740 1189 181 163
BtsI GCAGTG_nn'
Cuts at: 312 1338 2516 312
Size: 1026 1178 3069
Fragments arranged by size:
3069 1178 1026
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 485 520 485
Size: 35 5238
DraI TTT'AAA
Cuts at: 2272 2272
Size: 5273
DrdI GACnn_nn'nnGTC
Cuts at: 2979 4323 2979
Size: 1344 3929
EaeI y'GGCC_r
Cuts at: 137 148 428 689 1326 2858 3032 3423 3450
Size: 11 280 261 637 1532 174 391 27
Cuts at: 3450 4795 137
Size: 1345 615
Fragments arranged by size:
1532 1345 637 615 391 280 261 174
27 11
EagI C'GGCC_G
Cuts at: 2858 2858
Size: 5273
EarI CTCTTCn'nnn_
Cuts at: 1439 3296 3506 1439
Size: 1857 210 3206
Fragments arranged by size:
3206 1857 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3481 4223 4369 5083 3481
Size: 742 146 714 3671
Fragments arranged by size:
3671 742 714 146
EcoICRI GAG'CTC
Cuts at: 576 1344 1449 2285 576
Size: 768 105 836 3564
Fragments arranged by size:
3564 836 768 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 661 718 763 859 2209 3098 3530 3883 4545
Size: 57 45 96 1350 889 432 353 662
Cuts at: 4545 661
Size: 1389
Fragments arranged by size:
1389 1350 889 662 432 353 96 57
45
EcoO109I rG'GnC_Cy
Cuts at: 380 683 1839 2412 380
Size: 303 1156 573 3241
Fragments arranged by size:
3241 1156 573 303
EcoRI G'AATT_C
Cuts at: 2277 2277
Size: 5273
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3292 3292
Size: 5273
FspI TGC'GCA
Cuts at: 3054 3054
Size: 5273
HaeII r_GCGC'y
Cuts at: 1743 2707 2955 4185 4956 1743
Size: 964 248 1230 771 2060
Fragments arranged by size:
2060 1230 964 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 214 246 752 784 942 974 1072 1104 1532
Size: 32 506 32 158 32 98 32 428
Cuts at: 1532 1564 1641 1657 1673 1689 3115 3145 3147
Size: 32 77 16 16 16 1426 30 2
Cuts at: 3147 3177 214
Size: 30 2310
Fragments arranged by size:
2310 1426 506 428 158 98 77 32
32 32 32 32 30 30 16 16
16 2
HincII GTy'rAC
Cuts at: 2312 2312
Size: 5273
KasI G'GCGC_C
Cuts at: 2703 2951 2703
Size: 248 5025
KpnI G_GTAC'C
Cuts at: 2293 2293
Size: 5273
MfeI C'AATT_G
Cuts at: 1682 1682
Size: 5273
MluI A'CGCG_T
Cuts at: 4706 4706
Size: 5273
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 416 812 1979 4032 4216 416
Size: 396 1167 2053 184 1473
Fragments arranged by size:
2053 1473 1167 396 184
MscI TGG'CCA
Cuts at: 139 150 430 691 3034 139
Size: 11 280 261 2343 2378
Fragments arranged by size:
2378 2343 280 261 11
MslI CAynn'nnrTG
Cuts at: 472 487 536 1351 1850 2164 3262 3389 472
Size: 15 49 815 499 314 1098 127 2356
Fragments arranged by size:
2356 1098 815 499 314 127 49 15
MspA1I CmG'CkG
Cuts at: 282 369 1470 2384 2698 3058 3842 4087 282
Size: 87 1101 914 314 360 784 245 1468
Fragments arranged by size:
1468 1101 914 784 360 314 245 87
NaeI GCC'GGC
Cuts at: 3454 3454
Size: 5273
NarI GG'CG_CC
Cuts at: 2704 2952 2704
Size: 248 5025
NcoI C'CATG_G
Cuts at: 585 656 1190 585
Size: 71 534 4668
Fragments arranged by size:
4668 534 71
NgoMIV G'CCGG_C
Cuts at: 3452 3452
Size: 5273
NsiI A_TGCA'T
Cuts at: 879 879
Size: 5273
NspI r_CATG'y
Cuts at: 1239 2118 2326 2826 4429 1239
Size: 879 208 500 1603 2083
Fragments arranged by size:
2083 1603 879 500 208
PasI CC'CwG_GG
Cuts at: 1108 1108
Size: 5273
PciI A'CATG_T
Cuts at: 2114 4425 2114
Size: 2311 2962
PflMI CCAn_nnn'nTGG
Cuts at: 146 372 657 146
Size: 226 285 4762
Fragments arranged by size:
4762 285 226
PfoI T'CCnGG_A
Cuts at: 695 1212 2180 695
Size: 517 968 3788
Fragments arranged by size:
3788 968 517
PmeI GTTT'AAAC
Cuts at: 2272 2272
Size: 5273
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 3719
Size: 32 5241
PpuMI rG'GwC_Cy
Cuts at: 380 683 1839 380
Size: 303 1156 3814
Fragments arranged by size:
3814 1156 303
PspXI vC'TCGA_Gb
Cuts at: 1553 1553
Size: 5273
PstI C_TGCA'G
Cuts at: 1373 1901 2320 3005 1373
Size: 528 419 685 3641
Fragments arranged by size:
3641 685 528 419
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5273
PvuII CAG'CTG
Cuts at: 282 369 1470 2698 3058 282
Size: 87 1101 1228 360 2497
Fragments arranged by size:
2497 1228 1101 360 87
RsrII CG'GwC_CG
Cuts at: 3468 3468
Size: 5273
SacI G_AGCT'C
Cuts at: 578 1346 1451 2287 578
Size: 768 105 836 3564
Fragments arranged by size:
3564 836 768 105
SalI G'TCGA_C
Cuts at: 2310 2310
Size: 5273
SapI GCTCTTCn'nnn_
Cuts at: 1439 3296 3506 1439
Size: 1857 210 3206
Fragments arranged by size:
3206 1857 210
SbfI CC_TGCA'GG
Cuts at: 1373 1901 2320 1373
Size: 528 419 4326
Fragments arranged by size:
4326 528 419
ScaI AGT'ACT
Cuts at: 2071 4790 2071
Size: 2719 2554
SfcI C'TryA_G
Cuts at: 33 534 1369 1897 2316 3001 3969 4160 33
Size: 501 835 528 419 685 968 191 1146
Fragments arranged by size:
1146 968 835 685 528 501 419 191
SfoI GGC'GCC
Cuts at: 2705 2953 2705
Size: 248 5025
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5273
SmaI CCC'GGG
Cuts at: 355 2295 355
Size: 1940 3333
SmlI C'TyrA_G
Cuts at: 1553 1720 2428 3343 4057 4319 1553
Size: 167 708 915 714 262 2507
Fragments arranged by size:
2507 915 714 708 262 167
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5273
SphI G_CATG'C
Cuts at: 2326 2826 2326
Size: 500 4773
StyI C'CwwG_G
Cuts at: 585 656 1190 1226 2228 2407 2761 585
Size: 71 534 36 1002 179 354 3097
Fragments arranged by size:
3097 1002 534 354 179 71 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 215 1135 1448 2856 2989 3448 3657 215
Size: 920 313 1408 133 459 209 1831
Fragments arranged by size:
1831 1408 920 459 313 209 133
TatI w'GTAC_w
Cuts at: 284 1139 1241 2069 4788 284
Size: 855 102 828 2719 769
Fragments arranged by size:
2719 855 828 769 102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2207 2239 4790 4822 2207
Size: 32 2551 32 2658
Fragments arranged by size:
2658 2551 32 32
Tth111I GACn'n_nGTC
Cuts at: 127 384 2251 3070 127
Size: 257 1867 819 2330
Fragments arranged by size:
2330 1867 819 257
XbaI T'CTAG_A
Cuts at: 2304 2304
Size: 5273
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 423 1210 2139 2768 423
Size: 787 929 629 2928
Fragments arranged by size:
2928 929 787 629
XhoI C'TCGA_G
Cuts at: 1553 1553
Size: 5273
XmaI C'CCGG_G
Cuts at: 353 2293 353
Size: 1940 3333
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AloI ApaI AscI
AvrII BaeI BmtI BsiWI BsmBI BssHII BstEII BstZ17I
ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI PacI PmlI PshAI PsiI
PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XmnI ZraI