Restriction map for
pF1KB8408
(Circular) (Six-base) MAPSORT of: pF1KB8408.seq Check: 4127 from: 1 to: 4823
pF1KB8408 4823 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1861 1861
Size: 4823
Acc65I G'GTAC_C
Cuts at: 1839 1839
Size: 4823
AclI AA'CG_TT
Cuts at: 1817 4663 1817
Size: 2846 1977
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 354 698 1285 1469 2648 3080 3433 4095
Size: 98 344 587 184 1179 432 353 662
Cuts at: 4095 256
Size: 984
Fragments arranged by size:
1179 984 662 587 432 353 344 184
98
AflII C'TTAA_G
Cuts at: 1644 1644
Size: 4823
AflIII A'CryG_T
Cuts at: 266 1273 1654 2175 3975 4256 266
Size: 1007 381 521 1800 281 833
Fragments arranged by size:
1800 1007 833 521 381 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 647 1233 647
Size: 586 4237
AlwNI CAG_nnn'CTG
Cuts at: 2128 3566 2128
Size: 1438 3385
ApaLI G'TGCA_C
Cuts at: 1158 3661 1158
Size: 2503 2320
ApoI r'AATT_y
Cuts at: 1168 1508 1827 4176 4432 1168
Size: 340 319 2349 256 1559
Fragments arranged by size:
2349 1559 340 319 256
AseI AT'TA_AT
Cuts at: 20 787 1441 20
Size: 767 654 3402
Fragments arranged by size:
3402 767 654
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4823
AvaI C'yCGr_G
Cuts at: 1843 1843
Size: 4823
AvrII C'CTAG_G
Cuts at: 942 1113 942
Size: 171 4652
BamHI G'GATC_C
Cuts at: 1108 1848 1108
Size: 740 4083
BanI G'GyrC_C
Cuts at: 1839 2253 2501 2536 1839
Size: 414 248 35 4126
Fragments arranged by size:
4126 414 248 35
BanII G_rGCy'C
Cuts at: 1705 1837 2867 1705
Size: 132 1030 3661
Fragments arranged by size:
3661 1030 132
BbeI G_GCGC'C
Cuts at: 2257 2505 2257
Size: 248 4575
BbvCI CC'TCA_GC
Cuts at: 288 288
Size: 4823
BciVI GTATCCnnnnn_n'
Cuts at: 752 2721 3777 4823 752
Size: 1969 1056 1046 752
Fragments arranged by size:
1969 1056 1046 752
BclI T'GATC_A
Cuts at: 2343 2343
Size: 4823
BfrBI ATG'CAT
Cuts at: 1029 1029
Size: 4823
BglII A'GATC_T
Cuts at: 972 2338 4375 972
Size: 1366 2037 1420
Fragments arranged by size:
2037 1420 1366
BlpI GC'TnA_GC
Cuts at: 1704 1935 1704
Size: 231 4592
Bme1580I G_kGCm'C
Cuts at: 1162 1467 2448 2541 3665 1162
Size: 305 981 93 1124 2320
Fragments arranged by size:
2320 1124 981 305 93
BmgBI CAC'GTC
Cuts at: 1657 1657
Size: 4823
BmrI ACTGGGnnnn_n'
Cuts at: 523 2035 2116 2451 4595 523
Size: 1512 81 335 2144 751
Fragments arranged by size:
2144 1512 751 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3190 3190
Size: 4823
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 893 893
Size: 4823
Bpu10I CC'TnA_GC
Cuts at: 288 288
Size: 4823
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 808 1294 1999 2878 3592 3890 484
Size: 324 486 705 879 714 298 1417
Fragments arranged by size:
1417 879 714 705 486 324 298
BsaI GGTCTCn'nnnn_
Cuts at: 502 4527 502
Size: 4025 798
BsaAI yAC'GTr
Cuts at: 267 969 1274 2176 267
Size: 702 305 902 2914
Fragments arranged by size:
2914 902 702 305
BsaBI GATnn'nnATC
Cuts at: 6 2337 4374 6
Size: 2331 2037 455
Fragments arranged by size:
2331 2037 455
BsaHI Gr'CG_yC
Cuts at: 2254 2502 3204 4700 2254
Size: 248 702 1496 2377
Fragments arranged by size:
2377 1496 702 248
BsaWI w'CCGG_w
Cuts at: 853 2012 2533 3211 3622 3769 4043 853
Size: 1159 521 678 411 147 274 1633
Fragments arranged by size:
1633 1159 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 251 281 251
Size: 30 4793
BseYI C'CCAG_C
Cuts at: 2248 3006 3671 4079 2248
Size: 758 665 408 2992
Fragments arranged by size:
2992 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 229
Size: 4823
BsiEI CG_ry'CG
Cuts at: 104 2365 2411 3641 104
Size: 2261 46 1230 1286
Fragments arranged by size:
2261 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1162 1837 2615 2805 3665 1162
Size: 675 778 190 860 2320
Fragments arranged by size:
2320 860 778 675 190
BsmI GAATG_Cn'
Cuts at: 1400 1400
Size: 4823
Bsp1286I G_dGCh'C
Cuts at: 1162 1467 1705 1837 2448 2541 2615 2805 2867
Size: 305 238 132 611 93 74 190 62
Cuts at: 2867 3665 1162
Size: 798 2320
Fragments arranged by size:
2320 798 611 305 238 190 132 93
74 62
BspEI T'CCGG_A
Cuts at: 853 853
Size: 4823
BspHI T'CATG_A
Cuts at: 3255 3255
Size: 4823
BspMI ACCTGCnnnn'nnnn_
Cuts at: 600 1873 2087 2389 2770 600
Size: 1273 214 302 381 2653
Fragments arranged by size:
2653 1273 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3115 3169 4653 4818 50
Size: 3065 54 1484 165 55
Fragments arranged by size:
3065 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2735 2735
Size: 4823
BsrFI r'CCGG_y
Cuts at: 2821 3002 2821
Size: 181 4642
BsrGI T'GTAC_A
Cuts at: 271 841 271
Size: 570 4253
BssSI C'ACGA_G
Cuts at: 3094 3802 3094
Size: 708 4115
BstAPI GCAn_nnn'nTGC
Cuts at: 1296 2200 1296
Size: 904 3919
BstBI TT'CG_AA
Cuts at: 3184 3184
Size: 4823
BstYI r'GATC_y
Cuts at: 1 332 551 972 1108 1788 1848 2315 2338
Size: 331 219 421 136 680 60 467 23
Cuts at: 2338 2673 2919 3225 3237 3323 4375 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 680 467 449 421 335 331 306
246 219 136 86 60 23 12
Bsu36I CC'TnA_GG
Cuts at: 225 225
Size: 4823
BtgI C'CryG_G
Cuts at: 1318 1318
Size: 4823
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2773 2954 3117 2773
Size: 181 163 4479
Fragments arranged by size:
4479 181 163
BtsI GCAGTG_nn'
Cuts at: 1160 1247 1285 1467 2066 1160
Size: 87 38 182 599 3917
Fragments arranged by size:
3917 599 182 87 38
DraI TTT'AAA
Cuts at: 329 719 1822 329
Size: 390 1103 3330
Fragments arranged by size:
3330 1103 390
DraIII CAC_nnn'GTG
Cuts at: 1597 1597
Size: 4823
DrdI GACnn_nn'nnGTC
Cuts at: 2529 3873 2529
Size: 1344 3479
EaeI y'GGCC_r
Cuts at: 1576 2408 2582 2973 3000 4345 1576
Size: 832 174 391 27 1345 2054
Fragments arranged by size:
2054 1345 832 391 174 27
EagI C'GGCC_G
Cuts at: 2408 2408
Size: 4823
EarI CTCTTCn'nnn_
Cuts at: 2846 3056 2846
Size: 210 4613
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3031 3773 3919 4633 3031
Size: 742 146 714 3221
Fragments arranged by size:
3221 742 714 146
EcoICRI GAG'CTC
Cuts at: 1835 1835
Size: 4823
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 354 698 893 1285 1469 2648 3080 3433
Size: 98 344 195 392 184 1179 432 353
Cuts at: 3433 4095 256
Size: 662 984
Fragments arranged by size:
1179 984 662 432 392 353 344 195
184 98
EcoNI CCTnn'n_nnAGG
Cuts at: 228 719 228
Size: 491 4332
EcoO109I rG'GnC_Cy
Cuts at: 1962 1962
Size: 4823
EcoRI G'AATT_C
Cuts at: 1827 1827
Size: 4823
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2842 2842
Size: 4823
FspI TGC'GCA
Cuts at: 2604 2604
Size: 4823
HaeII r_GCGC'y
Cuts at: 2257 2505 3735 4506 2257
Size: 248 1230 771 2574
Fragments arranged by size:
2574 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1773 1805 2665 2695 2697 2727 1773
Size: 32 860 30 2 30 3869
Fragments arranged by size:
3869 860 32 30 30 2
HincII GTy'rAC
Cuts at: 1304 1862 1304
Size: 558 4265
HindIII A'AGCT_T
Cuts at: 366 1741 366
Size: 1375 3448
HpaI GTT'AAC
Cuts at: 1304 1304
Size: 4823
KasI G'GCGC_C
Cuts at: 2253 2501 2253
Size: 248 4575
KpnI G_GTAC'C
Cuts at: 1843 1843
Size: 4823
MluI A'CGCG_T
Cuts at: 4256 4256
Size: 4823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3582 3766 3582
Size: 184 4639
MscI TGG'CCA
Cuts at: 1578 2584 1578
Size: 1006 3817
MslI CAynn'nnrTG
Cuts at: 138 629 769 1519 2812 2939 138
Size: 491 140 750 1293 127 2022
Fragments arranged by size:
2022 1293 750 491 140 127
MspA1I CmG'CkG
Cuts at: 243 933 1934 2248 2608 3392 3637 243
Size: 690 1001 314 360 784 245 1429
Fragments arranged by size:
1429 1001 784 690 360 314 245
NaeI GCC'GGC
Cuts at: 3004 3004
Size: 4823
NarI GG'CG_CC
Cuts at: 2254 2502 2254
Size: 248 4575
NcoI C'CATG_G
Cuts at: 1318 1318
Size: 4823
NdeI CA'TA_TG
Cuts at: 1797 1797
Size: 4823
NgoMIV G'CCGG_C
Cuts at: 3002 3002
Size: 4823
NsiI A_TGCA'T
Cuts at: 1031 1031
Size: 4823
NspI r_CATG'y
Cuts at: 628 1294 1876 2376 3979 628
Size: 666 582 500 1603 1472
Fragments arranged by size:
1603 1472 666 582 500
PciI A'CATG_T
Cuts at: 3975 3975
Size: 4823
PmeI GTTT'AAAC
Cuts at: 1822 1822
Size: 4823
PmlI CAC'GTG
Cuts at: 1274 1274
Size: 4823
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3269 3301 3269
Size: 32 4791
PstI C_TGCA'G
Cuts at: 1222 1301 1870 2555 1222
Size: 79 569 685 3490
Fragments arranged by size:
3490 685 569 79
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4823
PvuII CAG'CTG
Cuts at: 243 933 2248 2608 243
Size: 690 1315 360 2458
Fragments arranged by size:
2458 1315 690 360
RsrII CG'GwC_CG
Cuts at: 3018 3018
Size: 4823
SacI G_AGCT'C
Cuts at: 1837 1837
Size: 4823
SalI G'TCGA_C
Cuts at: 1860 1860
Size: 4823
SapI GCTCTTCn'nnn_
Cuts at: 2846 3056 2846
Size: 210 4613
SbfI CC_TGCA'GG
Cuts at: 1870 1870
Size: 4823
ScaI AGT'ACT
Cuts at: 546 4340 546
Size: 3794 1029
SfcI C'TryA_G
Cuts at: 33 1218 1297 1753 1763 1866 2551 3519 3710
Size: 1185 79 456 10 103 685 968 191
Cuts at: 3710 33
Size: 1146
Fragments arranged by size:
1185 1146 968 685 456 191 103 79
10
SfoI GGC'GCC
Cuts at: 2255 2503 2255
Size: 248 4575
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4823
SmaI CCC'GGG
Cuts at: 1845 1845
Size: 4823
SmlI C'TyrA_G
Cuts at: 499 823 1309 1644 1978 2893 3607 3869 499
Size: 324 486 335 334 915 714 262 1453
Fragments arranged by size:
1453 915 714 486 335 334 324 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4823
SphI G_CATG'C
Cuts at: 1876 2376 1876
Size: 500 4323
StyI C'CwwG_G
Cuts at: 942 1113 1318 1721 1957 2311 942
Size: 171 205 403 236 354 3454
Fragments arranged by size:
3454 403 354 236 205 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 459 2406 2539 2998 3207 459
Size: 1947 133 459 209 2075
Fragments arranged by size:
2075 1947 459 209 133
TatI w'GTAC_w
Cuts at: 271 544 841 1342 1420 4338 271
Size: 273 297 501 78 2918 756
Fragments arranged by size:
2918 756 501 297 273 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 251 283 872 904 1277 1309 1644 1676 4340
Size: 32 589 32 373 32 335 32 2664
Cuts at: 4340 4372 251
Size: 32 702
Fragments arranged by size:
2664 702 589 373 335 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2620 2620
Size: 4823
XbaI T'CTAG_A
Cuts at: 1854 1854
Size: 4823
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1782 2318 114
Size: 1668 536 2619
Fragments arranged by size:
2619 1668 536
XmaI C'CCGG_G
Cuts at: 1843 1843
Size: 4823
XmnI GAAnn'nnTTC
Cuts at: 422 1400 422
Size: 978 3845
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PmeI PmlI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AloI ApaI
AscI BaeI BbsI BcgI BglI BmtI BseRI BsiWI
BsmBI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoRV
FseI FspAI MfeI NheI NotI NruI PacI PasI
PflMI PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI