Restriction map for
pF1KB8407
(Circular) (Six-base) MAPSORT of: pF1KB8407.seq Check: 3290 from: 1 to: 3995
pF1KB8407 3995 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 1033 1033
Size: 3995
Acc65I G'GTAC_C
Cuts at: 1011 1011
Size: 3995
AclI AA'CG_TT
Cuts at: 3835 3835
Size: 3995
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 1820 2252 2999 3267 619
Size: 1201 432 747 268 1347
Fragments arranged by size:
1347 1201 747 432 268
AflIII A'CryG_T
Cuts at: 835 1347 2451 3428 835
Size: 512 1104 977 1402
Fragments arranged by size:
1402 1104 977 512
AgeI A'CCGG_T
Cuts at: 2383 2383
Size: 3995
AleI CACnn'nnGTG
Cuts at: 616 616
Size: 3995
AlwNI CAG_nnn'CTG
Cuts at: 372 674 1300 2867 372
Size: 302 626 1567 1500
Fragments arranged by size:
1567 1500 626 302
ApaLI G'TGCA_C
Cuts at: 2765 2765
Size: 3995
ApoI r'AATT_y
Cuts at: 999 3348 3604 999
Size: 2349 256 1390
Fragments arranged by size:
2349 1390 256
AscI GG'CGCG_CC
Cuts at: 229 229
Size: 3995
AseI AT'TA_AT
Cuts at: 20 20
Size: 3995
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3995
AvaI C'yCGr_G
Cuts at: 1015 1015
Size: 3995
BamHI G'GATC_C
Cuts at: 1020 1020
Size: 3995
BanI G'GyrC_C
Cuts at: 256 1011 1425 1673 1708 256
Size: 755 414 248 35 2543
Fragments arranged by size:
2543 755 414 248 35
BanII G_rGCy'C
Cuts at: 1009 2039 1009
Size: 1030 2965
BbeI G_GCGC'C
Cuts at: 1429 1677 1429
Size: 248 3747
BbsI GAAGACnn'nnnn_
Cuts at: 580 992 580
Size: 412 3583
BciVI GTATCCnnnnn_n'
Cuts at: 125 1893 2654 3995 125
Size: 1768 761 1341 125
Fragments arranged by size:
1768 1341 761 125
BclI T'GATC_A
Cuts at: 1515 1515
Size: 3995
BglII A'GATC_T
Cuts at: 1510 3547 1510
Size: 2037 1958
BlpI GC'TnA_GC
Cuts at: 1107 1107
Size: 3995
Bme1580I G_kGCm'C
Cuts at: 261 1620 1713 2769 261
Size: 1359 93 1056 1487
Fragments arranged by size:
1487 1359 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 321 549 1207 1288 1623 3767 321
Size: 228 658 81 335 2144 549
Fragments arranged by size:
2144 658 549 335 228 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2362 2362
Size: 3995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 205
Size: 3995
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1171 2050 2542 2840 1171
Size: 879 492 298 2326
Fragments arranged by size:
2326 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 739 3699 739
Size: 2960 1035
BsaAI yAC'GTr
Cuts at: 1348 1348
Size: 3995
BsaBI GATnn'nnATC
Cuts at: 6 1509 3546 6
Size: 1503 2037 455
Fragments arranged by size:
2037 1503 455
BsaHI Gr'CG_yC
Cuts at: 1426 1674 2376 3872 1426
Size: 248 702 1496 1549
Fragments arranged by size:
1549 1496 702 248
BsaWI w'CCGG_w
Cuts at: 253 1184 1705 2383 2657 2804 3215 253
Size: 931 521 678 274 147 411 1033
Fragments arranged by size:
1033 931 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 260 1420 2178 2755 3251 260
Size: 1160 758 577 496 1004
Fragments arranged by size:
1160 1004 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 154
Size: 3995
BsiEI CG_ry'CG
Cuts at: 104 158 1537 1583 2791 104
Size: 54 1379 46 1208 1308
Fragments arranged by size:
1379 1308 1208 54 46
BsiHKAI G_wGCw'C
Cuts at: 290 1009 1787 1977 2769 290
Size: 719 778 190 792 1516
Fragments arranged by size:
1516 792 778 719 190
Bsp1286I G_dGCh'C
Cuts at: 261 290 1009 1620 1713 1787 1977 2039 2769
Size: 29 719 611 93 74 190 62 730
Cuts at: 2769 261
Size: 1487
Fragments arranged by size:
1487 730 719 611 190 93 74 62
29
BspEI T'CCGG_A
Cuts at: 3215 3215
Size: 3995
BspHI T'CATG_A
Cuts at: 3171 3171
Size: 3995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1045 1259 1561 1942 1045
Size: 214 302 381 3098
Fragments arranged by size:
3098 381 302 214
BsrBI CCG'CTC
Cuts at: 50 154 2287 2341 3825 3990 50
Size: 104 2133 54 1484 165 55
Fragments arranged by size:
2133 1484 165 104 55 54
BsrDI GCAATG_nn'
Cuts at: 1907 1907
Size: 3995
BsrFI r'CCGG_y
Cuts at: 123 264 1993 2174 2383 123
Size: 141 1729 181 209 1735
Fragments arranged by size:
1735 1729 209 181 141
BssHII G'CGCG_C
Cuts at: 229 229
Size: 3995
BssSI C'ACGA_G
Cuts at: 2266 2624 2266
Size: 358 3637
BstAPI GCAn_nnn'nTGC
Cuts at: 674 1372 674
Size: 698 3297
BstBI TT'CG_AA
Cuts at: 2356 2356
Size: 3995
BstXI CCAn_nnnn'nTGG
Cuts at: 618 618
Size: 3995
BstYI r'GATC_y
Cuts at: 1 1020 1487 1510 1845 2091 3103 3189 3201
Size: 1019 467 23 335 246 1012 86 12
Cuts at: 3201 3547 1
Size: 346 449
Fragments arranged by size:
1019 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 128 140 429 107
Size: 21 12 289 3673
Fragments arranged by size:
3673 289 21 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1945 2126 2289 1945
Size: 181 163 3651
Fragments arranged by size:
3651 181 163
BtsI GCAGTG_nn'
Cuts at: 1238 1238
Size: 3995
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 899 934 899
Size: 35 3960
DraI TTT'AAA
Cuts at: 994 994
Size: 3995
DraIII CAC_nnn'GTG
Cuts at: 329 329
Size: 3995
DrdI GACnn_nn'nnGTC
Cuts at: 793 1701 2559 793
Size: 908 858 2229
Fragments arranged by size:
2229 908 858
EaeI y'GGCC_r
Cuts at: 125 155 1580 1754 2145 2172 3517 125
Size: 30 1425 174 391 27 1345 603
Fragments arranged by size:
1425 1345 603 391 174 30 27
EagI C'GGCC_G
Cuts at: 155 1580 155
Size: 1425 2570
EarI CTCTTCn'nnn_
Cuts at: 319 406 475 2018 2228 319
Size: 87 69 1543 210 2086
Fragments arranged by size:
2086 1543 210 87 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 164 799 2203 2513 2659 3805 164
Size: 635 1404 310 146 1146 354
Fragments arranged by size:
1404 1146 635 354 310 146
EcoICRI GAG'CTC
Cuts at: 1007 1007
Size: 3995
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 619 1820 2252 2999 3267 205
Size: 414 1201 432 747 268 933
Fragments arranged by size:
1201 933 747 432 414 268
EcoO109I rG'GnC_Cy
Cuts at: 1134 1134
Size: 3995
EcoRI G'AATT_C
Cuts at: 999 999
Size: 3995
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2014 2014
Size: 3995
FspI TGC'GCA
Cuts at: 1776 1776
Size: 3995
HaeII r_GCGC'y
Cuts at: 1429 1677 2699 3678 1429
Size: 248 1022 979 1746
Fragments arranged by size:
1746 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1837 1867 1869 1899 1837
Size: 30 2 30 3933
Fragments arranged by size:
3933 30 30 2
HincII GTy'rAC
Cuts at: 1034 1034
Size: 3995
KasI G'GCGC_C
Cuts at: 1425 1673 1425
Size: 248 3747
KpnI G_GTAC'C
Cuts at: 1015 1015
Size: 3995
MluI A'CGCG_T
Cuts at: 3428 3428
Size: 3995
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2666 2850 2666
Size: 184 3811
MscI TGG'CCA
Cuts at: 1756 1756
Size: 3995
MslI CAynn'nnrTG
Cuts at: 616 1984 2111 616
Size: 1368 127 2500
Fragments arranged by size:
2500 1368 127
MspA1I CmG'CkG
Cuts at: 142 811 884 1106 1420 1780 2793 3038 142
Size: 669 73 222 314 360 1013 245 1099
Fragments arranged by size:
1099 1013 669 360 314 245 222 73
NaeI GCC'GGC
Cuts at: 125 2176 125
Size: 2051 1944
NarI GG'CG_CC
Cuts at: 1426 1674 1426
Size: 248 3747
NcoI C'CATG_G
Cuts at: 107 429 107
Size: 322 3673
NgoMIV G'CCGG_C
Cuts at: 123 2174 123
Size: 2051 1944
NspI r_CATG'y
Cuts at: 400 839 1048 1548 2455 400
Size: 439 209 500 907 1940
Fragments arranged by size:
1940 907 500 439 209
PasI CC'CwG_GG
Cuts at: 930 930
Size: 3995
PciI A'CATG_T
Cuts at: 835 2451 835
Size: 1616 2379
PflMI CCAn_nnn'nTGG
Cuts at: 267 372 435 267
Size: 105 63 3827
Fragments arranged by size:
3827 105 63
PmeI GTTT'AAAC
Cuts at: 994 994
Size: 3995
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3134 3166 3134
Size: 32 3963
PstI C_TGCA'G
Cuts at: 670 1042 1727 670
Size: 372 685 2938
Fragments arranged by size:
2938 685 372
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3995
PvuII CAG'CTG
Cuts at: 811 1420 1780 811
Size: 609 360 3026
Fragments arranged by size:
3026 609 360
RsrII CG'GwC_CG
Cuts at: 2190 2190
Size: 3995
SacI G_AGCT'C
Cuts at: 1009 1009
Size: 3995
SacII CC_GC'GG
Cuts at: 143 143
Size: 3995
SalI G'TCGA_C
Cuts at: 1032 1032
Size: 3995
SapI GCTCTTCn'nnn_
Cuts at: 475 2018 2228 475
Size: 1543 210 2242
Fragments arranged by size:
2242 1543 210
SbfI CC_TGCA'GG
Cuts at: 1042 1042
Size: 3995
ScaI AGT'ACT
Cuts at: 3512 3512
Size: 3995
SfcI C'TryA_G
Cuts at: 33 666 1038 1723 2716 2907 33
Size: 633 372 685 993 191 1121
Fragments arranged by size:
1121 993 685 633 372 191
SfoI GGC'GCC
Cuts at: 1427 1675 1427
Size: 248 3747
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3995
SmaI CCC'GGG
Cuts at: 1017 1017
Size: 3995
SmlI C'TyrA_G
Cuts at: 1150 2065 2557 2819 1150
Size: 915 492 262 2326
Fragments arranged by size:
2326 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3995
SphI G_CATG'C
Cuts at: 1048 1548 1048
Size: 500 3495
StyI C'CwwG_G
Cuts at: 107 429 980 1129 1483 107
Size: 322 551 149 354 2619
Fragments arranged by size:
2619 551 354 322 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 340 1578 1711 2170 2379 340
Size: 1238 133 459 209 1956
Fragments arranged by size:
1956 1238 459 209 133
TatI w'GTAC_w
Cuts at: 3510 3510
Size: 3995
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 789 821 3512 3544 789
Size: 32 2691 32 1240
Fragments arranged by size:
2691 1240 32 32
Tth111I GACn'n_nGTC
Cuts at: 697 1792 697
Size: 1095 2900
XbaI T'CTAG_A
Cuts at: 1026 1026
Size: 3995
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1490 1490
Size: 3995
XmaI C'CCGG_G
Cuts at: 1015 1015
Size: 3995
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI BstEII
BstZ17I Bsu36I ClaI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI