Restriction map for
pF1KB8405
(Circular) (Six-base) MAPSORT of: pF1KB8405.seq Check: 5384 from: 1 to: 5525
pF1KB8405 5525 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1116 2134 1116
Size: 1018 4507
AccI GT'mk_AC
Cuts at: 1856 2563 1856
Size: 707 4818
Acc65I G'GTAC_C
Cuts at: 2541 2541
Size: 5525
AclI AA'CG_TT
Cuts at: 2519 5365 2519
Size: 2846 2679
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1865 1939 3350 3782 4135 4797 1865
Size: 74 1411 432 353 662 2593
Fragments arranged by size:
2593 1411 662 432 353 74
AflIII A'CryG_T
Cuts at: 222 2877 4677 4958 222
Size: 2655 1800 281 789
Fragments arranged by size:
2655 1800 789 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1118 1118
Size: 5525
AlwNI CAG_nnn'CTG
Cuts at: 732 961 964 1060 1266 1300 2830 4268 732
Size: 229 3 96 206 34 1530 1438 1989
Fragments arranged by size:
1989 1530 1438 229 206 96 34 3
ApaI G_GGCC'C
Cuts at: 1688 1729 1688
Size: 41 5484
ApaLI G'TGCA_C
Cuts at: 4363 4363
Size: 5525
ApoI r'AATT_y
Cuts at: 1677 2529 4878 5134 1677
Size: 852 2349 256 2068
Fragments arranged by size:
2349 2068 852 256
AseI AT'TA_AT
Cuts at: 20 869 20
Size: 849 4676
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5525
AvaI C'yCGr_G
Cuts at: 2545 2545
Size: 5525
BamHI G'GATC_C
Cuts at: 1417 2550 1417
Size: 1133 4392
BanI G'GyrC_C
Cuts at: 1831 2541 2955 3203 3238 1831
Size: 710 414 248 35 4118
Fragments arranged by size:
4118 710 414 248 35
BanII G_rGCy'C
Cuts at: 1688 1729 2348 2539 3569 1688
Size: 41 619 191 1030 3644
Fragments arranged by size:
3644 1030 619 191 41
BbeI G_GCGC'C
Cuts at: 2959 3207 2959
Size: 248 5277
BbsI GAAGACnn'nnnn_
Cuts at: 113 2389 113
Size: 2276 3249
BciVI GTATCCnnnnn_n'
Cuts at: 1509 2273 3423 4479 5525 1509
Size: 764 1150 1056 1046 1509
Fragments arranged by size:
1509 1150 1056 1046 764
BclI T'GATC_A
Cuts at: 216 710 2417 3045 216
Size: 494 1707 628 2696
Fragments arranged by size:
2696 1707 628 494
BfrBI ATG'CAT
Cuts at: 1915 1974 1915
Size: 59 5466
BglI GCCn_nnn'nGGC
Cuts at: 1840 2503 1840
Size: 663 4862
BglII A'GATC_T
Cuts at: 3040 5077 3040
Size: 2037 3488
BlpI GC'TnA_GC
Cuts at: 1109 2065 2637 1109
Size: 956 572 3997
Fragments arranged by size:
3997 956 572
Bme1580I G_kGCm'C
Cuts at: 1688 1729 3150 3243 4367 1688
Size: 41 1421 93 1124 2846
Fragments arranged by size:
2846 1421 1124 93 41
BmrI ACTGGGnnnn_n'
Cuts at: 330 1205 1683 1732 2737 2818 3153 5297 330
Size: 875 478 49 1005 81 335 2144 558
Fragments arranged by size:
2144 1005 875 558 478 335 81 49
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 582 3892 582
Size: 3310 2215
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1075 1157 1075
Size: 82 5443
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 177 553 1220 2701 3580 4294 4592 177
Size: 376 667 1481 879 714 298 1110
Fragments arranged by size:
1481 1110 879 714 667 376 298
BsaI GGTCTCn'nnnn_
Cuts at: 1191 2186 5229 1191
Size: 995 3043 1487
Fragments arranged by size:
3043 1487 995
BsaAI yAC'GTr
Cuts at: 2878 2878
Size: 5525
BsaBI GATnn'nnATC
Cuts at: 6 2236 3039 5076 6
Size: 2230 803 2037 455
Fragments arranged by size:
2230 2037 803 455
BsaHI Gr'CG_yC
Cuts at: 1865 2956 3204 3906 5402 1865
Size: 1091 248 702 1496 1988
Fragments arranged by size:
1988 1496 1091 702 248
BsaWI w'CCGG_w
Cuts at: 2714 3235 3913 4324 4471 4745 2714
Size: 521 678 411 147 274 3494
Fragments arranged by size:
3494 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 292 1956 292
Size: 1664 3861
BseYI C'CCAG_C
Cuts at: 704 1338 2347 2950 3708 4373 4781 704
Size: 634 1009 603 758 665 408 1448
Fragments arranged by size:
1448 1009 758 665 634 603 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 173 229 173
Size: 56 5469
BsiEI CG_ry'CG
Cuts at: 104 1875 2116 3067 3113 4343 104
Size: 1771 241 951 46 1230 1286
Fragments arranged by size:
1771 1286 1230 951 241 46
BsiHKAI G_wGCw'C
Cuts at: 2371 2539 3317 3507 4367 2371
Size: 168 778 190 860 3529
Fragments arranged by size:
3529 860 778 190 168
BsmI GAATG_Cn'
Cuts at: 1915 1917 1915
Size: 2 5523
BsmBI CGTCTCn'nnnn_
Cuts at: 1322 1322
Size: 5525
Bsp1286I G_dGCh'C
Cuts at: 1688 1729 2348 2371 2539 3150 3243 3317 3507
Size: 41 619 23 168 611 93 74 190
Cuts at: 3507 3569 4367 1688
Size: 62 798 2846
Fragments arranged by size:
2846 798 619 611 190 168 93 74
62 41 23
BspHI T'CATG_A
Cuts at: 917 3957 917
Size: 3040 2485
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1116 2134 2575 2789 3091 3472 1116
Size: 1018 441 214 302 381 3169
Fragments arranged by size:
3169 1018 441 381 302 214
BsrBI CCG'CTC
Cuts at: 50 915 1108 3817 3871 5355 5520 50
Size: 865 193 2709 54 1484 165 55
Fragments arranged by size:
2709 1484 865 193 165 55 54
BsrDI GCAATG_nn'
Cuts at: 179 1784 3437 179
Size: 1605 1653 2267
Fragments arranged by size:
2267 1653 1605
BsrFI r'CCGG_y
Cuts at: 3523 3704 3523
Size: 181 5344
BssSI C'ACGA_G
Cuts at: 1850 3796 4504 1850
Size: 1946 708 2871
Fragments arranged by size:
2871 1946 708
BstAPI GCAn_nnn'nTGC
Cuts at: 849 961 964 1060 2902 849
Size: 112 3 96 1842 3472
Fragments arranged by size:
3472 1842 112 96 3
BstBI TT'CG_AA
Cuts at: 3886 3886
Size: 5525
BstYI r'GATC_y
Cuts at: 1 887 1417 1562 2002 2072 2550 3017 3040
Size: 886 530 145 440 70 478 467 23
Cuts at: 3040 3375 3621 3927 3939 4025 5077 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 886 530 478 467 449 440 335
306 246 145 86 70 23 12
BtgI C'CryG_G
Cuts at: 107 1869 107
Size: 1762 3763
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2282 3475 3656 3819 2282
Size: 1193 181 163 3988
Fragments arranged by size:
3988 1193 181 163
BtsI GCAGTG_nn'
Cuts at: 219 2490 2768 219
Size: 2271 278 2976
Fragments arranged by size:
2976 2271 278
DraI TTT'AAA
Cuts at: 2524 2524
Size: 5525
DraIII CAC_nnn'GTG
Cuts at: 1597 1597
Size: 5525
DrdI GACnn_nn'nnGTC
Cuts at: 3231 4575 3231
Size: 1344 4181
EaeI y'GGCC_r
Cuts at: 1115 1750 1819 1872 2333 3110 3284 3675 3702
Size: 635 69 53 461 777 174 391 27
Cuts at: 3702 5047 1115
Size: 1345 1593
Fragments arranged by size:
1593 1345 777 635 461 391 174 69
53 27
EagI C'GGCC_G
Cuts at: 1872 3110 1872
Size: 1238 4287
EarI CTCTTCn'nnn_
Cuts at: 200 991 1339 1407 1448 2045 3548 3758 200
Size: 791 348 68 41 597 1503 210 1967
Fragments arranged by size:
1967 1503 791 597 348 210 68 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2408 3733 4475 4621 5335 2408
Size: 1325 742 146 714 2598
Fragments arranged by size:
2598 1325 742 714 146
EcoICRI GAG'CTC
Cuts at: 2537 2537
Size: 5525
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1075 1157 1865 1939 3350 3782 4135 4797 1075
Size: 82 708 74 1411 432 353 662 1803
Fragments arranged by size:
1803 1411 708 662 432 353 82 74
EcoNI CCTnn'n_nnAGG
Cuts at: 425 425
Size: 5525
EcoO109I rG'GnC_Cy
Cuts at: 1684 1685 2664 1684
Size: 1 979 4545
Fragments arranged by size:
4545 979 1
EcoRI G'AATT_C
Cuts at: 2529 2529
Size: 5525
EcoRV GAT'ATC
Cuts at: 1473 1473
Size: 5525
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 137 511 2094 3544 137
Size: 374 1583 1450 2118
Fragments arranged by size:
2118 1583 1450 374
FspI TGC'GCA
Cuts at: 3306 3306
Size: 5525
HaeII r_GCGC'y
Cuts at: 483 2959 3207 4437 5208 483
Size: 2476 248 1230 771 800
Fragments arranged by size:
2476 1230 800 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 175 207 1462 1494 1547 1579 2166 2198 2455
Size: 32 1255 32 53 32 587 32 257
Cuts at: 2455 2487 3367 3397 3399 3429 175
Size: 32 880 30 2 30 2271
Fragments arranged by size:
2271 1255 880 587 257 53 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1444 1602 1694 2564 1444
Size: 158 92 870 4405
Fragments arranged by size:
4405 870 158 92
HindIII A'AGCT_T
Cuts at: 529 2146 529
Size: 1617 3908
HpaI GTT'AAC
Cuts at: 1694 1694
Size: 5525
KasI G'GCGC_C
Cuts at: 2955 3203 2955
Size: 248 5277
KpnI G_GTAC'C
Cuts at: 2545 2545
Size: 5525
MluI A'CGCG_T
Cuts at: 4958 4958
Size: 5525
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2006 2048 4284 4468 2006
Size: 42 2236 184 3063
Fragments arranged by size:
3063 2236 184 42
MscI TGG'CCA
Cuts at: 1117 1821 3286 1117
Size: 704 1465 3356
Fragments arranged by size:
3356 1465 704
MslI CAynn'nnrTG
Cuts at: 1126 3514 3641 1126
Size: 2388 127 3010
Fragments arranged by size:
3010 2388 127
MspA1I CmG'CkG
Cuts at: 157 708 945 961 1057 1113 1569 1643 1871
Size: 551 237 16 96 56 456 74 228
Cuts at: 1871 2636 2950 3310 4094 4339 157
Size: 765 314 360 784 245 1343
Fragments arranged by size:
1343 784 765 551 456 360 314 245
237 228 96 74 56 16
NaeI GCC'GGC
Cuts at: 3706 3706
Size: 5525
NarI GG'CG_CC
Cuts at: 2956 3204 2956
Size: 248 5277
NcoI C'CATG_G
Cuts at: 107 107
Size: 5525
NgoMIV G'CCGG_C
Cuts at: 3704 3704
Size: 5525
NsiI A_TGCA'T
Cuts at: 1917 1976 1917
Size: 59 5466
NspI r_CATG'y
Cuts at: 226 909 1125 1974 2578 3078 4681 226
Size: 683 216 849 604 500 1603 1070
Fragments arranged by size:
1603 1070 849 683 604 500 216
PciI A'CATG_T
Cuts at: 222 4677 222
Size: 4455 1070
PmeI GTTT'AAAC
Cuts at: 2524 2524
Size: 5525
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 671 703 3971 4003 671
Size: 32 3268 32 2193
Fragments arranged by size:
3268 2193 32 32
PspOMI G'GGCC_C
Cuts at: 1684 1725 1684
Size: 41 5484
PstI C_TGCA'G
Cuts at: 881 1648 1794 2572 3257 881
Size: 767 146 778 685 3149
Fragments arranged by size:
3149 778 767 685 146
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5525
PvuII CAG'CTG
Cuts at: 157 708 961 1057 1113 1643 2950 3310 157
Size: 551 253 96 56 530 1307 360 2372
Fragments arranged by size:
2372 1307 551 530 360 253 96 56
RsrII CG'GwC_CG
Cuts at: 3720 3720
Size: 5525
SacI G_AGCT'C
Cuts at: 2539 2539
Size: 5525
SacII CC_GC'GG
Cuts at: 1872 1872
Size: 5525
SalI G'TCGA_C
Cuts at: 2562 2562
Size: 5525
SapI GCTCTTCn'nnn_
Cuts at: 2045 3548 3758 2045
Size: 1503 210 3812
Fragments arranged by size:
3812 1503 210
SbfI CC_TGCA'GG
Cuts at: 2572 2572
Size: 5525
ScaI AGT'ACT
Cuts at: 5042 5042
Size: 5525
SexAI A'CCwGG_T
Cuts at: 1011 1011
Size: 5525
SfcI C'TryA_G
Cuts at: 33 877 1644 1790 1801 1857 2478 2568 3253
Size: 844 767 146 11 56 621 90 685
Cuts at: 3253 4221 4412 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 844 767 685 621 191 146
90 56 11
SfoI GGC'GCC
Cuts at: 2957 3205 2957
Size: 248 5277
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5525
SmaI CCC'GGG
Cuts at: 2547 2547
Size: 5525
SmlI C'TyrA_G
Cuts at: 192 532 1235 2680 3595 4309 4571 192
Size: 340 703 1445 915 714 262 1146
Fragments arranged by size:
1445 1146 915 714 703 340 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5525
SphI G_CATG'C
Cuts at: 1125 2578 3078 1125
Size: 1453 500 3572
Fragments arranged by size:
3572 1453 500
StuI AGG'CCT
Cuts at: 1960 1960
Size: 5525
StyI C'CwwG_G
Cuts at: 107 2659 3013 107
Size: 2552 354 2619
Fragments arranged by size:
2619 2552 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1339 2130 3108 3241 3700 3909 1339
Size: 791 978 133 459 209 2955
Fragments arranged by size:
2955 978 791 459 209 133
TatI w'GTAC_w
Cuts at: 5040 5040
Size: 5525
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5042 5074 5042
Size: 32 5493
Tth111I GACn'n_nGTC
Cuts at: 3322 3322
Size: 5525
XbaI T'CTAG_A
Cuts at: 2556 2556
Size: 5525
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1626 2371 3020 1626
Size: 745 649 4131
Fragments arranged by size:
4131 745 649
XmaI C'CCGG_G
Cuts at: 2545 2545
Size: 5525
XmnI GAAnn'nnTTC
Cuts at: 120 1333 1915 120
Size: 1213 582 3730
Fragments arranged by size:
3730 1213 582
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AloI AscI
AvrII BaeI BbvCI BcgI BmgBI BmtI Bpu10I BsaXI
BsiWI BspEI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI FseI FspAI MfeI NdeI NheI NotI
NruI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI