Restriction map for
pF1KB8402
(Circular) (Six-base) MAPSORT of: pF1KB8402.seq Check: 622 from: 1 to: 5459
pF1KB8402 5459 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AatII G_ACGT'C
Cuts at: 644 644
Size: 5459
AccI GT'mk_AC
Cuts at: 2497 2497
Size: 5459
Acc65I G'GTAC_C
Cuts at: 2475 2475
Size: 5459
AclI AA'CG_TT
Cuts at: 5299 5299
Size: 5459
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 294 480 1747 1771 1783 1939 2188 3284 3716
Size: 186 1267 24 12 156 249 1096 432
Cuts at: 3716 4069 4731 294
Size: 353 662 1022
Fragments arranged by size:
1267 1096 1022 662 432 353 249 186
156 24 12
AfeI AGC'GCT
Cuts at: 1018 1237 1321 1741 1993 1018
Size: 219 84 420 252 4484
Fragments arranged by size:
4484 420 252 219 84
AflIII A'CryG_T
Cuts at: 748 2811 4611 4892 748
Size: 2063 1800 281 1315
Fragments arranged by size:
2063 1800 1315 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1363 1395 1615 1647 1783 1815 1363
Size: 32 220 32 136 32 5007
Fragments arranged by size:
5007 220 136 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1268 1512 1527 1725 1947 2031 2196 2764 4202
Size: 244 15 198 222 84 165 568 1438
Cuts at: 4202 1268
Size: 2525
Fragments arranged by size:
2525 1438 568 244 222 198 165 84
15
ApaI G_GGCC'C
Cuts at: 907 1211 907
Size: 304 5155
ApaLI G'TGCA_C
Cuts at: 999 1114 1696 1864 4297 999
Size: 115 582 168 2433 2161
Fragments arranged by size:
2433 2161 582 168 115
ApoI r'AATT_y
Cuts at: 423 766 2463 4812 5068 423
Size: 343 1697 2349 256 814
Fragments arranged by size:
2349 1697 814 343 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5459
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5459
AvaI C'yCGr_G
Cuts at: 520 809 1119 2290 2479 520
Size: 289 310 1171 189 3500
Fragments arranged by size:
3500 1171 310 289 189
BamHI G'GATC_C
Cuts at: 481 2484 481
Size: 2003 3456
BanI G'GyrC_C
Cuts at: 1052 1517 2270 2475 2889 3137 3172 1052
Size: 465 753 205 414 248 35 3339
Fragments arranged by size:
3339 753 465 414 248 205 35
BanII G_rGCy'C
Cuts at: 308 529 552 907 1211 1602 2473 3503 308
Size: 221 23 355 304 391 871 1030 2264
Fragments arranged by size:
2264 1030 871 391 355 304 221 23
BbeI G_GCGC'C
Cuts at: 2893 3141 2893
Size: 248 5211
BbvCI CC'TCA_GC
Cuts at: 2266 2266
Size: 5459
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 501 535 1389 1423 501
Size: 34 854 34 4537
Fragments arranged by size:
4537 854 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3357 4413 5459 3357
Size: 1056 1046 3357
Fragments arranged by size:
3357 1056 1046
BclI T'GATC_A
Cuts at: 2979 2979
Size: 5459
BfrBI ATG'CAT
Cuts at: 369 369
Size: 5459
BglI GCCn_nnn'nGGC
Cuts at: 123 1754 2192 2295 123
Size: 1631 438 103 3287
Fragments arranged by size:
3287 1631 438 103
BglII A'GATC_T
Cuts at: 2974 5011 2974
Size: 2037 3422
BlpI GC'TnA_GC
Cuts at: 1570 1933 2182 2416 2571 1570
Size: 363 249 234 155 4458
Fragments arranged by size:
4458 363 249 234 155
Bme1580I G_kGCm'C
Cuts at: 907 1003 1118 1211 1558 1700 1868 1894 3084
Size: 96 115 93 347 142 168 26 1190
Cuts at: 3084 3177 4301 907
Size: 93 1124 2065
Fragments arranged by size:
2065 1190 1124 347 168 142 115 96
93 93 26
BmrI ACTGGGnnnn_n'
Cuts at: 569 990 1021 2671 2752 3087 5231 569
Size: 421 31 1650 81 335 2144 797
Fragments arranged by size:
2144 1650 797 421 335 81 31
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 824 3826 824
Size: 3002 2457
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1089 1304 1603 1089
Size: 215 299 4945
Fragments arranged by size:
4945 299 215
Bpu10I CC'TnA_GC
Cuts at: 2266 2266
Size: 5459
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2635 3514 4228 4526 2635
Size: 879 714 298 3568
Fragments arranged by size:
3568 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1368 1872 5163 1368
Size: 504 3291 1664
Fragments arranged by size:
3291 1664 504
BsaAI yAC'GTr
Cuts at: 751 2812 751
Size: 2061 3398
BsaBI GATnn'nnATC
Cuts at: 6 2973 5010 6
Size: 2967 2037 455
Fragments arranged by size:
2967 2037 455
BsaHI Gr'CG_yC
Cuts at: 542 641 2890 3138 3840 5336 542
Size: 99 2249 248 702 1496 665
Fragments arranged by size:
2249 1496 702 665 248 99
BsaWI w'CCGG_w
Cuts at: 743 827 911 1466 1977 2648 3169 3847 4258
Size: 84 84 555 511 671 521 678 411
Cuts at: 4258 4405 4679 743
Size: 147 274 1523
Fragments arranged by size:
1523 678 671 555 521 511 411 274
147 84 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1363 1393 1615 1645 1783 1813 1363
Size: 30 222 30 138 30 5009
Fragments arranged by size:
5009 222 138 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 345 542 345
Size: 197 5262
BseYI C'CCAG_C
Cuts at: 662 958 2884 3642 4307 4715 662
Size: 296 1926 758 665 408 1406
Fragments arranged by size:
1926 1406 758 665 408 296
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1513 1933 2017 2182 1513
Size: 420 84 165 4790
Fragments arranged by size:
4790 420 165 84
BsiEI CG_ry'CG
Cuts at: 104 3001 3047 4277 104
Size: 2897 46 1230 1286
Fragments arranged by size:
2897 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 661 1003 1118 1436 1688 1700 1856 1868 2105
Size: 342 115 318 252 12 156 12 237
Cuts at: 2105 2357 2473 3251 3441 4301 661
Size: 252 116 778 190 860 1819
Fragments arranged by size:
1819 860 778 342 318 252 252 237
190 156 116 115 12 12
BsmBI CGTCTCn'nnnn_
Cuts at: 249 778 249
Size: 529 4930
Bsp1286I G_dGCh'C
Cuts at: 308 529 552 661 907 1003 1118 1211 1436
Size: 221 23 109 246 96 115 93 225
Cuts at: 1436 1558 1602 1688 1700 1856 1868 1894 2105
Size: 122 44 86 12 156 12 26 211
Cuts at: 2105 2357 2473 3084 3177 3251 3441 3503 4301
Size: 252 116 611 93 74 190 62 798
Cuts at: 4301 308
Size: 1466
Fragments arranged by size:
1466 798 611 252 246 225 221 211
190 156 122 116 115 109 96 93
93 86 74 62 44 26 23 12
12
BspEI T'CCGG_A
Cuts at: 911 1977 911
Size: 1066 4393
BspHI T'CATG_A
Cuts at: 3891 3891
Size: 5459
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1335 2509 2723 3025 3406 1335
Size: 1174 214 302 381 3388
Fragments arranged by size:
3388 1174 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3751 3805 5289 5454 50
Size: 3701 54 1484 165 55
Fragments arranged by size:
3701 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1308 2438 3371 1308
Size: 1130 933 3396
Fragments arranged by size:
3396 1130 933
BsrFI r'CCGG_y
Cuts at: 1049 1074 1604 3457 3638 1049
Size: 25 530 1853 181 2870
Fragments arranged by size:
2870 1853 530 181 25
BssSI C'ACGA_G
Cuts at: 943 1803 3730 4438 943
Size: 860 1927 708 1964
Fragments arranged by size:
1964 1927 860 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1007 2836 1007
Size: 1829 3630
BstBI TT'CG_AA
Cuts at: 3820 3820
Size: 5459
BstXI CCAn_nnnn'nTGG
Cuts at: 618 618
Size: 5459
BstYI r'GATC_y
Cuts at: 1 180 456 481 621 2484 2951 2974 3309
Size: 179 276 25 140 1863 467 23 335
Cuts at: 3309 3555 3861 3873 3959 5011 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1863 1052 467 449 335 306 276 246
179 140 86 25 23 12
BtgI C'CryG_G
Cuts at: 107 796 1095 107
Size: 689 299 4471
Fragments arranged by size:
4471 689 299
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3409 3590 3753 3409
Size: 181 163 5115
Fragments arranged by size:
5115 181 163
BtsI GCAGTG_nn'
Cuts at: 1116 1423 2220 2702 1116
Size: 307 797 482 3873
Fragments arranged by size:
3873 797 482 307
DraI TTT'AAA
Cuts at: 2458 2458
Size: 5459
DrdI GACnn_nn'nnGTC
Cuts at: 3165 4509 3165
Size: 1344 4115
EaeI y'GGCC_r
Cuts at: 3044 3218 3609 3636 4981 3044
Size: 174 391 27 1345 3522
Fragments arranged by size:
3522 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3044 3044
Size: 5459
EarI CTCTTCn'nnn_
Cuts at: 3482 3692 3482
Size: 210 5249
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 796 844 1315 1567 1735 1903 2152 3667
Size: 669 48 471 252 168 168 249 1515
Cuts at: 3667 4409 4555 5269 127
Size: 742 146 714 317
Fragments arranged by size:
1515 742 714 669 471 317 252 249
168 168 146 48
EcoICRI GAG'CTC
Cuts at: 2471 2471
Size: 5459
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 294 480 1089 1304 1603 1747 1771 1783 1939
Size: 186 609 215 299 144 24 12 156
Cuts at: 1939 2188 3284 3716 4069 4731 294
Size: 249 1096 432 353 662 1022
Fragments arranged by size:
1096 1022 662 609 432 353 299 249
215 186 156 144 24 12
EcoNI CCTnn'n_nnAGG
Cuts at: 506 1147 1342 506
Size: 641 195 4623
Fragments arranged by size:
4623 641 195
EcoO109I rG'GnC_Cy
Cuts at: 607 755 903 904 1100 1207 1208 1712 1964
Size: 148 148 1 196 107 1 504 252
Cuts at: 1964 2193 2297 2598 607
Size: 229 104 301 3468
Fragments arranged by size:
3468 504 301 252 229 196 148 148
107 104 1 1
EcoRI G'AATT_C
Cuts at: 423 2463 423
Size: 2040 3419
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3478 3478
Size: 5459
FseI GG_CCGG'CC
Cuts at: 1078 1078
Size: 5459
FspI TGC'GCA
Cuts at: 1307 3240 1307
Size: 1933 3526
FspAI rTGC'GCAy
Cuts at: 1307 1307
Size: 5459
HaeII r_GCGC'y
Cuts at: 1020 1189 1239 1253 1323 1743 1947 1995 2893
Size: 169 50 14 70 420 204 48 898
Cuts at: 2893 3141 4371 5142 1020
Size: 248 1230 771 1337
Fragments arranged by size:
1337 1230 898 771 420 248 204 169
70 50 48 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3301 3331 3333 3363 3301
Size: 30 2 30 5397
Fragments arranged by size:
5397 30 30 2
HincII GTy'rAC
Cuts at: 2498 2498
Size: 5459
HindIII A'AGCT_T
Cuts at: 2344 2344
Size: 5459
KasI G'GCGC_C
Cuts at: 2889 3137 2889
Size: 248 5211
KpnI G_GTAC'C
Cuts at: 2479 2479
Size: 5459
MluI A'CGCG_T
Cuts at: 4892 4892
Size: 5459
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 963 4218 4402 963
Size: 3255 184 2020
Fragments arranged by size:
3255 2020 184
MscI TGG'CCA
Cuts at: 3220 3220
Size: 5459
MslI CAynn'nnrTG
Cuts at: 3448 3575 3448
Size: 127 5332
MspA1I CmG'CkG
Cuts at: 396 1097 1451 1509 1524 1703 1871 1929 2028
Size: 701 354 58 15 179 168 58 99
Cuts at: 2028 2039 2178 2570 2884 3244 4028 4273 396
Size: 11 139 392 314 360 784 245 1582
Fragments arranged by size:
1582 784 701 392 360 354 314 245
179 168 139 99 58 58 15 11
NaeI GCC'GGC
Cuts at: 1076 3640 1076
Size: 2564 2895
NarI GG'CG_CC
Cuts at: 2890 3138 2890
Size: 248 5211
NcoI C'CATG_G
Cuts at: 107 107
Size: 5459
NgoMIV G'CCGG_C
Cuts at: 1074 3638 1074
Size: 2564 2895
NsiI A_TGCA'T
Cuts at: 371 371
Size: 5459
NspI r_CATG'y
Cuts at: 217 1593 2265 2512 3012 4615 217
Size: 1376 672 247 500 1603 1061
Fragments arranged by size:
1603 1376 1061 672 500 247
PciI A'CATG_T
Cuts at: 4611 4611
Size: 5459
PflMI CCAn_nnn'nTGG
Cuts at: 1564 1564
Size: 5459
PfoI T'CCnGG_A
Cuts at: 283 283
Size: 5459
PmeI GTTT'AAAC
Cuts at: 2458 2458
Size: 5459
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1363 1395 1615 1647 1783 1815 3905 3937 1363
Size: 32 220 32 136 32 2090 32 2885
Fragments arranged by size:
2885 2090 220 136 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 607 607
Size: 5459
PshAI GACnn'nnGTC
Cuts at: 241 241
Size: 5459
PspOMI G'GGCC_C
Cuts at: 903 1207 903
Size: 304 5155
PstI C_TGCA'G
Cuts at: 1193 1273 1346 1390 2506 3191 1193
Size: 80 73 44 1116 685 3461
Fragments arranged by size:
3461 1116 685 80 73 44
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5459
PvuII CAG'CTG
Cuts at: 396 1509 1929 2028 2178 2884 3244 396
Size: 1113 420 99 150 706 360 2611
Fragments arranged by size:
2611 1113 706 420 360 150 99
RsrII CG'GwC_CG
Cuts at: 3654 3654
Size: 5459
SacI G_AGCT'C
Cuts at: 2473 2473
Size: 5459
SacII CC_GC'GG
Cuts at: 1098 1098
Size: 5459
SalI G'TCGA_C
Cuts at: 2496 2496
Size: 5459
SapI GCTCTTCn'nnn_
Cuts at: 3482 3692 3482
Size: 210 5249
SbfI CC_TGCA'GG
Cuts at: 1273 1390 2506 1273
Size: 117 1116 4226
Fragments arranged by size:
4226 1116 117
ScaI AGT'ACT
Cuts at: 4976 4976
Size: 5459
SfcI C'TryA_G
Cuts at: 33 1189 1269 1342 1386 1638 2502 3187 4155
Size: 1156 80 73 44 252 864 685 968
Cuts at: 4155 4346 33
Size: 191 1146
Fragments arranged by size:
1156 1146 968 864 685 252 191 80
73 44
SfiI GGCCn_nnn'nGGCC
Cuts at: 2295 2295
Size: 5459
SfoI GGC'GCC
Cuts at: 2891 3139 2891
Size: 248 5211
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5459
SgrAI Cr'CCGG_yG
Cuts at: 1049 1049
Size: 5459
SmaI CCC'GGG
Cuts at: 2481 2481
Size: 5459
SmlI C'TyrA_G
Cuts at: 2614 3529 4243 4505 2614
Size: 915 714 262 3568
Fragments arranged by size:
3568 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5459
SphI G_CATG'C
Cuts at: 1593 2512 3012 1593
Size: 919 500 4040
Fragments arranged by size:
4040 919 500
StuI AGG'CCT
Cuts at: 501 2214 501
Size: 1713 3746
StyI C'CwwG_G
Cuts at: 107 507 670 2593 2947 107
Size: 400 163 1923 354 2619
Fragments arranged by size:
2619 1923 400 354 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 676 1678 3042 3175 3634 3843 676
Size: 1002 1364 133 459 209 2292
Fragments arranged by size:
2292 1364 1002 459 209 133
TatI w'GTAC_w
Cuts at: 2364 4974 2364
Size: 2610 2849
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2359 2391 4976 5008 2359
Size: 32 2585 32 2810
Fragments arranged by size:
2810 2585 32 32
Tth111I GACn'n_nGTC
Cuts at: 3256 3256
Size: 5459
XbaI T'CTAG_A
Cuts at: 183 249 2490 183
Size: 66 2241 3152
Fragments arranged by size:
3152 2241 66
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 475 2954 475
Size: 2479 2980
XmaI C'CCGG_G
Cuts at: 2479 2479
Size: 5459
XmnI GAAnn'nnTTC
Cuts at: 1462 2326 1462
Size: 864 4595
ZraI GAC'GTC
Cuts at: 642 642
Size: 5459
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AflII AgeI AhdI AleI AlfI AscI AvrII
BaeI BbsI BmgBI BmtI BsiWI BsmI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV HpaI
MfeI NdeI NheI NotI NruI PacI PasI PmlI
PsiI PspXI PsrI SanDI SexAI SnaBI SrfI SspI
SwaI XhoI