Restriction map for
pF1KB8400
(Circular) (Six-base) MAPSORT of: pF1KB8400.seq Check: 200 from: 1 to: 3746
pF1KB8400 3746 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 784 784
Size: 3746
Acc65I G'GTAC_C
Cuts at: 762 762
Size: 3746
AclI AA'CG_TT
Cuts at: 3586 3586
Size: 3746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1571 2003 2356 3018 1571
Size: 432 353 662 2299
Fragments arranged by size:
2299 662 432 353
AflIII A'CryG_T
Cuts at: 1098 2898 3179 1098
Size: 1800 281 1665
Fragments arranged by size:
1800 1665 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 737 737
Size: 3746
AlwNI CAG_nnn'CTG
Cuts at: 247 1051 2489 247
Size: 804 1438 1504
Fragments arranged by size:
1504 1438 804
ApaLI G'TGCA_C
Cuts at: 2584 2584
Size: 3746
ApoI r'AATT_y
Cuts at: 750 3099 3355 750
Size: 2349 256 1141
Fragments arranged by size:
2349 1141 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3746
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
AvaI C'yCGr_G
Cuts at: 766 766
Size: 3746
BamHI G'GATC_C
Cuts at: 771 771
Size: 3746
BanI G'GyrC_C
Cuts at: 543 762 1176 1424 1459 543
Size: 219 414 248 35 2830
Fragments arranged by size:
2830 414 248 219 35
BanII G_rGCy'C
Cuts at: 218 760 1790 218
Size: 542 1030 2174
Fragments arranged by size:
2174 1030 542
BbeI G_GCGC'C
Cuts at: 1180 1428 1180
Size: 248 3498
BciVI GTATCCnnnnn_n'
Cuts at: 304 1644 2700 3746 304
Size: 1340 1056 1046 304
Fragments arranged by size:
1340 1056 1046 304
BclI T'GATC_A
Cuts at: 561 1266 561
Size: 705 3041
BglI GCCn_nnn'nGGC
Cuts at: 552 552
Size: 3746
BglII A'GATC_T
Cuts at: 1261 3298 1261
Size: 2037 1709
BlpI GC'TnA_GC
Cuts at: 858 858
Size: 3746
Bme1580I G_kGCm'C
Cuts at: 1371 1464 2588 1371
Size: 93 1124 2529
Fragments arranged by size:
2529 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 958 1039 1374 3518 958
Size: 81 335 2144 1186
Fragments arranged by size:
2144 1186 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2113 2113
Size: 3746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 214 232 165
Size: 49 18 3679
Fragments arranged by size:
3679 49 18
Bpu10I CC'TnA_GC
Cuts at: 113 217 113
Size: 104 3642
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1801 2515 2813 922
Size: 879 714 298 1855
Fragments arranged by size:
1855 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3450 3450
Size: 3746
BsaAI yAC'GTr
Cuts at: 1099 1099
Size: 3746
BsaBI GATnn'nnATC
Cuts at: 6 1260 3297 6
Size: 1254 2037 455
Fragments arranged by size:
2037 1254 455
BsaHI Gr'CG_yC
Cuts at: 1177 1425 2127 3623 1177
Size: 248 702 1496 1300
Fragments arranged by size:
1496 1300 702 248
BsaWI w'CCGG_w
Cuts at: 935 1456 2134 2545 2692 2966 935
Size: 521 678 411 147 274 1715
Fragments arranged by size:
1715 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 150 153 150
Size: 3 3743
BseYI C'CCAG_C
Cuts at: 419 1171 1929 2594 3002 419
Size: 752 758 665 408 1163
Fragments arranged by size:
1163 758 752 665 408
BsiEI CG_ry'CG
Cuts at: 104 1288 1334 2564 104
Size: 1184 46 1230 1286
Fragments arranged by size:
1286 1230 1184 46
BsiHKAI G_wGCw'C
Cuts at: 218 597 760 1538 1728 2588 218
Size: 379 163 778 190 860 1376
Fragments arranged by size:
1376 860 778 379 190 163
BsmI GAATG_Cn'
Cuts at: 684 684
Size: 3746
Bsp1286I G_dGCh'C
Cuts at: 218 597 760 1371 1464 1538 1728 1790 2588
Size: 379 163 611 93 74 190 62 798
Cuts at: 2588 218
Size: 1376
Fragments arranged by size:
1376 798 611 379 190 163 93 74
62
BspHI T'CATG_A
Cuts at: 2178 2178
Size: 3746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 796 1010 1312 1693 796
Size: 214 302 381 2849
Fragments arranged by size:
2849 381 302 214
BsrBI CCG'CTC
Cuts at: 50 158 179 2038 2092 3576 3741 50
Size: 108 21 1859 54 1484 165 55
Fragments arranged by size:
1859 1484 165 108 55 54 21
BsrDI GCAATG_nn'
Cuts at: 1658 1658
Size: 3746
BsrFI r'CCGG_y
Cuts at: 1744 1925 1744
Size: 181 3565
BssSI C'ACGA_G
Cuts at: 2017 2725 2017
Size: 708 3038
BstAPI GCAn_nnn'nTGC
Cuts at: 247 1123 247
Size: 876 2870
BstBI TT'CG_AA
Cuts at: 2107 2107
Size: 3746
BstYI r'GATC_y
Cuts at: 1 771 1238 1261 1596 1842 2148 2160 2246
Size: 770 467 23 335 246 306 12 86
Cuts at: 2246 3298 1
Size: 1052 449
Fragments arranged by size:
1052 770 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 167 107
Size: 60 3686
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1696 1877 2040 1696
Size: 181 163 3402
Fragments arranged by size:
3402 181 163
BtsI GCAGTG_nn'
Cuts at: 989 989
Size: 3746
DraI TTT'AAA
Cuts at: 745 745
Size: 3746
DrdI GACnn_nn'nnGTC
Cuts at: 1452 2796 1452
Size: 1344 2402
EaeI y'GGCC_r
Cuts at: 1331 1505 1896 1923 3268 1331
Size: 174 391 27 1345 1809
Fragments arranged by size:
1809 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1331 1331
Size: 3746
EarI CTCTTCn'nnn_
Cuts at: 1769 1979 1769
Size: 210 3536
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1954 2696 2842 3556 1954
Size: 742 146 714 2144
Fragments arranged by size:
2144 742 714 146
EcoICRI GAG'CTC
Cuts at: 216 758 216
Size: 542 3204
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 214 232 1571 2003 2356 3018 165
Size: 49 18 1339 432 353 662 893
Fragments arranged by size:
1339 893 662 432 353 49 18
EcoO109I rG'GnC_Cy
Cuts at: 885 885
Size: 3746
EcoRI G'AATT_C
Cuts at: 750 750
Size: 3746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 289 1765 289
Size: 1476 2270
FspI TGC'GCA
Cuts at: 1527 1527
Size: 3746
HaeII r_GCGC'y
Cuts at: 150 1180 1428 2658 3429 150
Size: 1030 248 1230 771 467
Fragments arranged by size:
1230 1030 771 467 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1588 1618 1620 1650 1588
Size: 30 2 30 3684
Fragments arranged by size:
3684 30 30 2
HincII GTy'rAC
Cuts at: 785 785
Size: 3746
HindIII A'AGCT_T
Cuts at: 227 227
Size: 3746
KasI G'GCGC_C
Cuts at: 1176 1424 1176
Size: 248 3498
KpnI G_GTAC'C
Cuts at: 766 766
Size: 3746
MluI A'CGCG_T
Cuts at: 3179 3179
Size: 3746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2505 2689 2505
Size: 184 3562
MscI TGG'CCA
Cuts at: 1507 1507
Size: 3746
MslI CAynn'nnrTG
Cuts at: 1735 1862 1735
Size: 127 3619
MspA1I CmG'CkG
Cuts at: 210 857 1171 1531 2315 2560 210
Size: 647 314 360 784 245 1396
Fragments arranged by size:
1396 784 647 360 314 245
NaeI GCC'GGC
Cuts at: 1927 1927
Size: 3746
NarI GG'CG_CC
Cuts at: 1177 1425 1177
Size: 248 3498
NcoI C'CATG_G
Cuts at: 107 167 107
Size: 60 3686
NgoMIV G'CCGG_C
Cuts at: 1925 1925
Size: 3746
NspI r_CATG'y
Cuts at: 445 533 799 1299 2902 445
Size: 88 266 500 1603 1289
Fragments arranged by size:
1603 1289 500 266 88
PciI A'CATG_T
Cuts at: 2898 2898
Size: 3746
PmeI GTTT'AAAC
Cuts at: 745 745
Size: 3746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2192 2224 2192
Size: 32 3714
PstI C_TGCA'G
Cuts at: 793 1478 793
Size: 685 3061
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3746
PvuII CAG'CTG
Cuts at: 210 1171 1531 210
Size: 961 360 2425
Fragments arranged by size:
2425 961 360
RsrII CG'GwC_CG
Cuts at: 1941 1941
Size: 3746
SacI G_AGCT'C
Cuts at: 218 760 218
Size: 542 3204
SalI G'TCGA_C
Cuts at: 783 783
Size: 3746
SapI GCTCTTCn'nnn_
Cuts at: 1769 1979 1769
Size: 210 3536
SbfI CC_TGCA'GG
Cuts at: 793 793
Size: 3746
ScaI AGT'ACT
Cuts at: 3263 3263
Size: 3746
SfcI C'TryA_G
Cuts at: 33 522 789 1474 2442 2633 33
Size: 489 267 685 968 191 1146
Fragments arranged by size:
1146 968 685 489 267 191
SfoI GGC'GCC
Cuts at: 1178 1426 1178
Size: 248 3498
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
SmaI CCC'GGG
Cuts at: 768 768
Size: 3746
SmlI C'TyrA_G
Cuts at: 901 1816 2530 2792 901
Size: 915 714 262 1855
Fragments arranged by size:
1855 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3746
SphI G_CATG'C
Cuts at: 445 799 1299 445
Size: 354 500 2892
Fragments arranged by size:
2892 500 354
SspI AAT'ATT
Cuts at: 648 648
Size: 3746
StyI C'CwwG_G
Cuts at: 107 167 880 1234 107
Size: 60 713 354 2619
Fragments arranged by size:
2619 713 354 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1329 1462 1921 2130 1329
Size: 133 459 209 2945
Fragments arranged by size:
2945 459 209 133
TatI w'GTAC_w
Cuts at: 3261 3261
Size: 3746
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 3714
Tth111I GACn'n_nGTC
Cuts at: 1543 1543
Size: 3746
XbaI T'CTAG_A
Cuts at: 777 777
Size: 3746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 189 1241 189
Size: 1052 2694
XmaI C'CCGG_G
Cuts at: 766 766
Size: 3746
XmnI GAAnn'nnTTC
Cuts at: 272 272
Size: 3746
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI BsaXI BsgI BsiWI BsmBI BspEI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI