Restriction map for
pF1KB8396
(Circular) (Six-base) MAPSORT of: pF1KB8396.seq Check: 1383 from: 1 to: 4505
pF1KB8396 4505 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1543 1543
Size: 4505
Acc65I G'GTAC_C
Cuts at: 1521 1521
Size: 4505
AclI AA'CG_TT
Cuts at: 4345 4345
Size: 4505
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2330 2762 3509 3777 2330
Size: 432 747 268 3058
Fragments arranged by size:
3058 747 432 268
AflIII A'CryG_T
Cuts at: 392 1857 2961 3938 392
Size: 1465 1104 977 959
Fragments arranged by size:
1465 1104 977 959
AgeI A'CCGG_T
Cuts at: 2893 2893
Size: 4505
AhdI GACnn_n'nnGTC
Cuts at: 1299 1299
Size: 4505
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1118 1118
Size: 4505
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 379 411 379
Size: 32 4473
AlwNI CAG_nnn'CTG
Cuts at: 1810 3377 1810
Size: 1567 2938
ApaI G_GGCC'C
Cuts at: 757 801 757
Size: 44 4461
ApaLI G'TGCA_C
Cuts at: 3275 3275
Size: 4505
ApoI r'AATT_y
Cuts at: 1509 3858 4114 1509
Size: 2349 256 1900
Fragments arranged by size:
2349 1900 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4505
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4505
AvaI C'yCGr_G
Cuts at: 163 240 330 555 768 801 1036 1525 163
Size: 77 90 225 213 33 235 489 3143
Fragments arranged by size:
3143 489 235 225 213 90 77 33
BamHI G'GATC_C
Cuts at: 669 1530 669
Size: 861 3644
BanI G'GyrC_C
Cuts at: 565 1521 1935 2183 2218 565
Size: 956 414 248 35 2852
Fragments arranged by size:
2852 956 414 248 35
BanII G_rGCy'C
Cuts at: 329 644 757 801 1047 1222 1519 2549 329
Size: 315 113 44 246 175 297 1030 2285
Fragments arranged by size:
2285 1030 315 297 246 175 113 44
BbeI G_GCGC'C
Cuts at: 569 1939 2187 569
Size: 1370 248 2887
Fragments arranged by size:
2887 1370 248
BbsI GAAGACnn'nnnn_
Cuts at: 675 675
Size: 4505
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4471
BciVI GTATCCnnnnn_n'
Cuts at: 2403 3164 4505 2403
Size: 761 1341 2403
Fragments arranged by size:
2403 1341 761
BclI T'GATC_A
Cuts at: 2025 2025
Size: 4505
BglI GCCn_nnn'nGGC
Cuts at: 652 748 772 827 652
Size: 96 24 55 4330
Fragments arranged by size:
4330 96 55 24
BglII A'GATC_T
Cuts at: 2020 4057 2020
Size: 2037 2468
BlpI GC'TnA_GC
Cuts at: 1617 1617
Size: 4505
Bme1580I G_kGCm'C
Cuts at: 593 725 744 757 801 2130 2223 3279 593
Size: 132 19 13 44 1329 93 1056 1819
Fragments arranged by size:
1819 1329 1056 132 93 44 19 13
BmgBI CAC'GTC
Cuts at: 125 942 1177 125
Size: 817 235 3453
Fragments arranged by size:
3453 817 235
BmrI ACTGGGnnnn_n'
Cuts at: 421 543 1717 1798 2133 4277 421
Size: 122 1174 81 335 2144 649
Fragments arranged by size:
2144 1174 649 335 122 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 189 336 2872 189
Size: 147 2536 1822
Fragments arranged by size:
2536 1822 147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 505 1294 1390 427
Size: 78 789 96 3542
Fragments arranged by size:
3542 789 96 78
Bpu10I CC'TnA_GC
Cuts at: 886 1318 886
Size: 432 4073
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 1168 1236 1681 2560 3052 3350 376
Size: 792 68 445 879 492 298 1531
Fragments arranged by size:
1531 879 792 492 445 298 68
BsaI GGTCTCn'nnnn_
Cuts at: 4209 4209
Size: 4505
BsaAI yAC'GTr
Cuts at: 1122 1329 1858 1122
Size: 207 529 3769
Fragments arranged by size:
3769 529 207
BsaBI GATnn'nnATC
Cuts at: 6 2019 4056 6
Size: 2013 2037 455
Fragments arranged by size:
2037 2013 455
BsaHI Gr'CG_yC
Cuts at: 566 1168 1936 2184 2886 4382 566
Size: 602 768 248 702 1496 689
Fragments arranged by size:
1496 768 702 689 602 248
BsaWI w'CCGG_w
Cuts at: 871 1694 2215 2893 3167 3314 3725 871
Size: 823 521 678 274 147 411 1651
Fragments arranged by size:
1651 823 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 379 409 379
Size: 30 4475
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 400 960 1429 400
Size: 560 469 3476
Fragments arranged by size:
3476 560 469
BseYI C'CCAG_C
Cuts at: 295 335 789 1930 2688 3265 3761 295
Size: 40 454 1141 758 577 496 1039
Fragments arranged by size:
1141 1039 758 577 496 454 40
BsiEI CG_ry'CG
Cuts at: 104 383 519 615 656 764 831 2047 2093
Size: 279 136 96 41 108 67 1216 46
Cuts at: 2093 3301 104
Size: 1208 1308
Fragments arranged by size:
1308 1216 1208 279 136 108 96 67
46 41
BsiHKAI G_wGCw'C
Cuts at: 317 644 1421 1519 2297 2487 3279 317
Size: 327 777 98 778 190 792 1543
Fragments arranged by size:
1543 792 778 777 327 190 98
Bsp1286I G_dGCh'C
Cuts at: 317 329 593 644 725 744 757 801 1047
Size: 12 264 51 81 19 13 44 246
Cuts at: 1047 1222 1421 1519 2130 2223 2297 2487 2549
Size: 175 199 98 611 93 74 190 62
Cuts at: 2549 3279 317
Size: 730 1543
Fragments arranged by size:
1543 730 611 264 246 199 190 175
98 93 81 74 62 51 44 19
13 12
BspEI T'CCGG_A
Cuts at: 871 3725 871
Size: 2854 1651
BspHI T'CATG_A
Cuts at: 3681 3681
Size: 4505
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1555 1769 2071 2452 1555
Size: 214 302 381 3608
Fragments arranged by size:
3608 381 302 214
BsrBI CCG'CTC
Cuts at: 50 461 1182 2797 2851 4335 4500 50
Size: 411 721 1615 54 1484 165 55
Fragments arranged by size:
1615 1484 721 411 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2417 2417
Size: 4505
BsrFI r'CCGG_y
Cuts at: 535 562 610 2503 2684 2893 535
Size: 27 48 1893 181 209 2147
Fragments arranged by size:
2147 1893 209 181 48 27
BssHII G'CGCG_C
Cuts at: 489 489
Size: 4505
BssSI C'ACGA_G
Cuts at: 2776 3134 2776
Size: 358 4147
BstAPI GCAn_nnn'nTGC
Cuts at: 146 1467 1882 146
Size: 1321 415 2769
Fragments arranged by size:
2769 1321 415
BstBI TT'CG_AA
Cuts at: 2866 2866
Size: 4505
BstYI r'GATC_y
Cuts at: 1 669 1530 1997 2020 2355 2601 3613 3699
Size: 668 861 467 23 335 246 1012 86
Cuts at: 3699 3711 4057 1
Size: 12 346 449
Fragments arranged by size:
1012 861 668 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 300 519 677 300
Size: 219 158 4128
Fragments arranged by size:
4128 219 158
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2455 2636 2799 2455
Size: 181 163 4161
Fragments arranged by size:
4161 181 163
BtsI GCAGTG_nn'
Cuts at: 1748 1748
Size: 4505
DraI TTT'AAA
Cuts at: 1504 1504
Size: 4505
DrdI GACnn_nn'nnGTC
Cuts at: 2211 3069 2211
Size: 858 3647
EaeI y'GGCC_r
Cuts at: 380 516 612 653 734 828 2090 2264 2655
Size: 136 96 41 81 94 1262 174 391
Cuts at: 2655 2682 4027 380
Size: 27 1345 858
Fragments arranged by size:
1345 1262 858 391 174 136 96 94
81 41 27
EagI C'GGCC_G
Cuts at: 380 516 612 653 828 2090 380
Size: 136 96 41 175 1262 2795
Fragments arranged by size:
2795 1262 175 136 96 41
EarI CTCTTCn'nnn_
Cuts at: 908 920 2528 2738 908
Size: 12 1608 210 2675
Fragments arranged by size:
2675 1608 210 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 459 2713 3023 3169 4315 459
Size: 2254 310 146 1146 649
Fragments arranged by size:
2254 1146 649 310 146
EcoICRI GAG'CTC
Cuts at: 642 1517 642
Size: 875 3630
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 505 1294 1390 2330 2762 3509 3777 427
Size: 78 789 96 940 432 747 268 1155
Fragments arranged by size:
1155 940 789 747 432 268 96 78
EcoO109I rG'GnC_Cy
Cuts at: 754 797 798 858 1033 1644 754
Size: 43 1 60 175 611 3615
Fragments arranged by size:
3615 611 175 60 43 1
EcoRI G'AATT_C
Cuts at: 1509 1509
Size: 4505
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2524 2524
Size: 4505
FspI TGC'GCA
Cuts at: 2286 2286
Size: 4505
HaeII r_GCGC'y
Cuts at: 569 718 1281 1287 1939 2187 3209 4188 569
Size: 149 563 6 652 248 1022 979 886
Fragments arranged by size:
1022 979 886 652 563 248 149 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1285 1317 2347 2377 2379 2409 1285
Size: 32 1030 30 2 30 3381
Fragments arranged by size:
3381 1030 32 30 30 2
HincII GTy'rAC
Cuts at: 1544 1544
Size: 4505
KasI G'GCGC_C
Cuts at: 565 1935 2183 565
Size: 1370 248 2887
Fragments arranged by size:
2887 1370 248
KpnI G_GTAC'C
Cuts at: 1525 1525
Size: 4505
MluI A'CGCG_T
Cuts at: 392 3938 392
Size: 3546 959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 998 3176 3360 998
Size: 2178 184 2143
Fragments arranged by size:
2178 2143 184
MscI TGG'CCA
Cuts at: 2266 2266
Size: 4505
MslI CAynn'nnrTG
Cuts at: 346 1006 1100 2494 2621 346
Size: 660 94 1394 127 2230
Fragments arranged by size:
2230 1394 660 127 94
MspA1I CmG'CkG
Cuts at: 288 521 842 1616 1930 2290 3303 3548 288
Size: 233 321 774 314 360 1013 245 1245
Fragments arranged by size:
1245 1013 774 360 321 314 245 233
NaeI GCC'GGC
Cuts at: 564 612 2686 564
Size: 48 2074 2383
Fragments arranged by size:
2383 2074 48
NarI GG'CG_CC
Cuts at: 566 1936 2184 566
Size: 1370 248 2887
Fragments arranged by size:
2887 1370 248
NgoMIV G'CCGG_C
Cuts at: 562 610 2684 562
Size: 48 2074 2383
Fragments arranged by size:
2383 2074 48
NotI GC'GGCC_GC
Cuts at: 828 828
Size: 4505
NspI r_CATG'y
Cuts at: 456 1558 2058 2965 456
Size: 1102 500 907 1996
Fragments arranged by size:
1996 1102 907 500
PasI CC'CwG_GG
Cuts at: 1029 1029
Size: 4505
PciI A'CATG_T
Cuts at: 2961 2961
Size: 4505
PfoI T'CCnGG_A
Cuts at: 443 1271 443
Size: 828 3677
PmeI GTTT'AAAC
Cuts at: 1504 1504
Size: 4505
PmlI CAC'GTG
Cuts at: 1329 1329
Size: 4505
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 379 411 3644 3676 379
Size: 32 3233 32 1208
Fragments arranged by size:
3233 1208 32 32
PpuMI rG'GwC_Cy
Cuts at: 1033 1033
Size: 4505
PspOMI G'GGCC_C
Cuts at: 753 797 753
Size: 44 4461
PspXI vC'TCGA_Gb
Cuts at: 163 163
Size: 4505
PstI C_TGCA'G
Cuts at: 512 1552 2237 512
Size: 1040 685 2780
Fragments arranged by size:
2780 1040 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4505
PvuII CAG'CTG
Cuts at: 1930 2290 1930
Size: 360 4145
RsrII CG'GwC_CG
Cuts at: 2700 2700
Size: 4505
SacI G_AGCT'C
Cuts at: 644 1519 644
Size: 875 3630
SacII CC_GC'GG
Cuts at: 522 522
Size: 4505
SalI G'TCGA_C
Cuts at: 1542 1542
Size: 4505
SanDI GG'GwC_CC
Cuts at: 1033 1033
Size: 4505
SapI GCTCTTCn'nnn_
Cuts at: 2528 2738 2528
Size: 210 4295
SbfI CC_TGCA'GG
Cuts at: 1552 1552
Size: 4505
ScaI AGT'ACT
Cuts at: 4022 4022
Size: 4505
SfcI C'TryA_G
Cuts at: 33 178 508 1548 2233 3226 3417 33
Size: 145 330 1040 685 993 191 1121
Fragments arranged by size:
1121 1040 993 685 330 191 145
SfoI GGC'GCC
Cuts at: 567 1937 2185 567
Size: 1370 248 2887
Fragments arranged by size:
2887 1370 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4505
SmaI CCC'GGG
Cuts at: 242 557 803 1038 1527 242
Size: 315 246 235 489 3220
Fragments arranged by size:
3220 489 315 246 235
SmlI C'TyrA_G
Cuts at: 163 355 1183 1215 1660 2575 3067 3329 163
Size: 192 828 32 445 915 492 262 1339
Fragments arranged by size:
1339 915 828 492 445 262 192 32
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4505
SphI G_CATG'C
Cuts at: 1558 2058 1558
Size: 500 4005
StyI C'CwwG_G
Cuts at: 980 1361 1639 1993 980
Size: 381 278 354 3492
Fragments arranged by size:
3492 381 354 278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 403 1289 2088 2221 2680 2889 403
Size: 886 799 133 459 209 2019
Fragments arranged by size:
2019 886 799 459 209 133
TatI w'GTAC_w
Cuts at: 4020 4020
Size: 4505
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 439 471 996 1028 4022 4054 439
Size: 32 525 32 2994 32 890
Fragments arranged by size:
2994 890 525 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 943 2302 943
Size: 1359 3146
XbaI T'CTAG_A
Cuts at: 1536 1536
Size: 4505
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2000 2000
Size: 4505
XhoI C'TCGA_G
Cuts at: 163 163
Size: 4505
XmaI C'CCGG_G
Cuts at: 240 555 801 1036 1525 240
Size: 315 246 235 489 3220
Fragments arranged by size:
3220 489 315 246 235
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NotI NspI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AscI AvrII BaeI
BbvCI BfrBI BmtI BsgI BsiWI BsmI BsmBI BsrGI
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NruI NsiI PacI PflMI PshAI PsiI PsrI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XmnI ZraI