Restriction map for
pF1KB8389
(Circular) (Six-base) MAPSORT of: pF1KB8389.seq Check: 714 from: 1 to: 4292
pF1KB8389 4292 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 149 245 1330 149
Size: 96 1085 3111
Fragments arranged by size:
3111 1085 96
Acc65I G'GTAC_C
Cuts at: 1308 1308
Size: 4292
AclI AA'CG_TT
Cuts at: 4132 4132
Size: 4292
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1249 2117 2549 3296 3564 1249
Size: 868 432 747 268 1977
Fragments arranged by size:
1977 868 747 432 268
AfeI AGC'GCT
Cuts at: 1075 1075
Size: 4292
AflIII A'CryG_T
Cuts at: 935 1644 2748 3725 935
Size: 709 1104 977 1502
Fragments arranged by size:
1502 1104 977 709
AgeI A'CCGG_T
Cuts at: 2680 2680
Size: 4292
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1108 1140 1108
Size: 32 4260
AlwNI CAG_nnn'CTG
Cuts at: 688 1101 1597 3164 688
Size: 413 496 1567 1816
Fragments arranged by size:
1816 1567 496 413
ApaI G_GGCC'C
Cuts at: 392 640 392
Size: 248 4044
ApaLI G'TGCA_C
Cuts at: 3062 3062
Size: 4292
ApoI r'AATT_y
Cuts at: 1296 3645 3901 1296
Size: 2349 256 1687
Fragments arranged by size:
2349 1687 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4292
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4292
AvaI C'yCGr_G
Cuts at: 165 370 763 792 1142 1312 165
Size: 205 393 29 350 170 3145
Fragments arranged by size:
3145 393 350 205 170 29
BamHI G'GATC_C
Cuts at: 1253 1317 1253
Size: 64 4228
BanI G'GyrC_C
Cuts at: 664 678 718 1151 1308 1722 1970 2005 664
Size: 14 40 433 157 414 248 35 2951
Fragments arranged by size:
2951 433 414 248 157 40 35 14
BanII G_rGCy'C
Cuts at: 127 296 392 559 640 864 913 962 1265
Size: 169 96 167 81 224 49 49 303
Cuts at: 1265 1306 2336 127
Size: 41 1030 2083
Fragments arranged by size:
2083 1030 303 224 169 167 96 81
49 49 41
BbeI G_GCGC'C
Cuts at: 1155 1726 1974 1155
Size: 571 248 3473
Fragments arranged by size:
3473 571 248
BciVI GTATCCnnnnn_n'
Cuts at: 2190 2951 4292 2190
Size: 761 1341 2190
Fragments arranged by size:
2190 1341 761
BclI T'GATC_A
Cuts at: 1812 1812
Size: 4292
BglI GCCn_nnn'nGGC
Cuts at: 276 450 619 795 844 276
Size: 174 169 176 49 3724
Fragments arranged by size:
3724 176 174 169 49
BglII A'GATC_T
Cuts at: 1807 3844 1807
Size: 2037 2255
BlpI GC'TnA_GC
Cuts at: 1404 1404
Size: 4292
Bme1580I G_kGCm'C
Cuts at: 392 640 667 1917 2010 3066 392
Size: 248 27 1250 93 1056 1618
Fragments arranged by size:
1618 1250 1056 248 93 27
BmrI ACTGGGnnnn_n'
Cuts at: 1504 1585 1920 4064 1504
Size: 81 335 2144 1732
Fragments arranged by size:
2144 1732 335 81
BmtI G_CTAG'C
Cuts at: 826 826
Size: 4292
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 978 2659 978
Size: 1681 2611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 654 1123 1129 654
Size: 469 6 3817
Fragments arranged by size:
3817 469 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1468 2347 2839 3137 1468
Size: 879 492 298 2623
Fragments arranged by size:
2623 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3996 3996
Size: 4292
BsaAI yAC'GTr
Cuts at: 1645 1645
Size: 4292
BsaBI GATnn'nnATC
Cuts at: 6 1806 3843 6
Size: 1800 2037 455
Fragments arranged by size:
2037 1800 455
BsaHI Gr'CG_yC
Cuts at: 1152 1723 1971 2673 4169 1152
Size: 571 248 702 1496 1275
Fragments arranged by size:
1496 1275 702 571 248
BsaWI w'CCGG_w
Cuts at: 1250 1481 2002 2680 2954 3101 3512 1250
Size: 231 521 678 274 147 411 2030
Fragments arranged by size:
2030 678 521 411 274 231 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1102 1108 1132 1138 1102
Size: 6 24 6 4256
Fragments arranged by size:
4256 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 940 970 940
Size: 30 4262
BseYI C'CCAG_C
Cuts at: 306 467 500 1717 2475 3052 3548 306
Size: 161 33 1217 758 577 496 1050
Fragments arranged by size:
1217 1050 758 577 496 161 33
BsiEI CG_ry'CG
Cuts at: 104 839 950 1834 1880 3088 104
Size: 735 111 884 46 1208 1308
Fragments arranged by size:
1308 1208 884 735 111 46
BsiHKAI G_wGCw'C
Cuts at: 529 559 962 1265 1306 2084 2274 3066 529
Size: 30 403 303 41 778 190 792 1755
Fragments arranged by size:
1755 792 778 403 303 190 41 30
Bsp1286I G_dGCh'C
Cuts at: 127 296 392 529 559 640 667 864 913
Size: 169 96 137 30 81 27 197 49
Cuts at: 913 962 1265 1306 1917 2010 2084 2274 2336
Size: 49 303 41 611 93 74 190 62
Cuts at: 2336 3066 127
Size: 730 1353
Fragments arranged by size:
1353 730 611 303 197 190 169 137
96 93 81 74 62 49 49 41
30 27
BspEI T'CCGG_A
Cuts at: 3512 3512
Size: 4292
BspHI T'CATG_A
Cuts at: 3468 3468
Size: 4292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 257 275 1342 1556 1858 2239 257
Size: 18 1067 214 302 381 2310
Fragments arranged by size:
2310 1067 381 302 214 18
BsrBI CCG'CTC
Cuts at: 50 1149 2584 2638 4122 4287 50
Size: 1099 1435 54 1484 165 55
Fragments arranged by size:
1484 1435 1099 165 55 54
BsrDI GCAATG_nn'
Cuts at: 327 2204 327
Size: 1877 2415
BsrFI r'CCGG_y
Cuts at: 2290 2471 2680 2290
Size: 181 209 3902
Fragments arranged by size:
3902 209 181
BssHII G'CGCG_C
Cuts at: 752 752
Size: 4292
BssSI C'ACGA_G
Cuts at: 522 2563 2921 522
Size: 2041 358 1893
Fragments arranged by size:
2041 1893 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1669 1669
Size: 4292
BstBI TT'CG_AA
Cuts at: 2653 2653
Size: 4292
BstYI r'GATC_y
Cuts at: 1 1253 1317 1784 1807 2142 2388 3400 3486
Size: 1252 64 467 23 335 246 1012 86
Cuts at: 3486 3498 3844 1
Size: 12 346 449
Fragments arranged by size:
1252 1012 467 449 346 335 246 86
64 23 12
BtgI C'CryG_G
Cuts at: 234 631 950 234
Size: 397 319 3576
Fragments arranged by size:
3576 397 319
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2242 2423 2586 2242
Size: 181 163 3948
Fragments arranged by size:
3948 181 163
BtsI GCAGTG_nn'
Cuts at: 1535 1535
Size: 4292
DraI TTT'AAA
Cuts at: 1291 1291
Size: 4292
DraIII CAC_nnn'GTG
Cuts at: 524 524
Size: 4292
DrdI GACnn_nn'nnGTC
Cuts at: 1998 2856 1998
Size: 858 3434
EaeI y'GGCC_r
Cuts at: 602 836 947 1877 2051 2442 2469 3814 602
Size: 234 111 930 174 391 27 1345 1080
Fragments arranged by size:
1345 1080 930 391 234 174 111 27
EagI C'GGCC_G
Cuts at: 836 947 1877 836
Size: 111 930 3251
Fragments arranged by size:
3251 930 111
EarI CTCTTCn'nnn_
Cuts at: 301 545 2315 2525 301
Size: 244 1770 210 2068
Fragments arranged by size:
2068 1770 244 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 595 688 2500 2810 2956 4102 595
Size: 93 1812 310 146 1146 785
Fragments arranged by size:
1812 1146 785 310 146 93
EcoICRI GAG'CTC
Cuts at: 557 960 1263 1304 557
Size: 403 303 41 3545
Fragments arranged by size:
3545 403 303 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 654 1123 1129 1249 2117 2549 3296 3564 654
Size: 469 6 120 868 432 747 268 1382
Fragments arranged by size:
1382 868 747 469 432 268 120 6
EcoNI CCTnn'n_nnAGG
Cuts at: 706 706
Size: 4292
EcoO109I rG'GnC_Cy
Cuts at: 367 389 636 1431 367
Size: 22 247 795 3228
Fragments arranged by size:
3228 795 247 22
EcoRI G'AATT_C
Cuts at: 1296 1296
Size: 4292
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2311 2311
Size: 4292
FspI TGC'GCA
Cuts at: 2073 2073
Size: 4292
HaeII r_GCGC'y
Cuts at: 511 1077 1155 1726 1974 2996 3975 511
Size: 566 78 571 248 1022 979 828
Fragments arranged by size:
1022 979 828 571 566 248 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 316 348 2134 2164 2166 2196 316
Size: 32 1786 30 2 30 2412
Fragments arranged by size:
2412 1786 32 30 30 2
HincII GTy'rAC
Cuts at: 150 1331 150
Size: 1181 3111
KasI G'GCGC_C
Cuts at: 1151 1722 1970 1151
Size: 571 248 3473
Fragments arranged by size:
3473 571 248
KpnI G_GTAC'C
Cuts at: 1312 1312
Size: 4292
MluI A'CGCG_T
Cuts at: 935 3725 935
Size: 2790 1502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1045 2963 3147 1045
Size: 1918 184 2190
Fragments arranged by size:
2190 1918 184
MscI TGG'CCA
Cuts at: 2053 2053
Size: 4292
MslI CAynn'nnrTG
Cuts at: 2281 2408 2281
Size: 127 4165
MspA1I CmG'CkG
Cuts at: 344 633 674 740 902 952 1403 1717 2077
Size: 289 41 66 162 50 451 314 360
Cuts at: 2077 3090 3335 344
Size: 1013 245 1301
Fragments arranged by size:
1301 1013 451 360 314 289 245 162
66 50 41
NaeI GCC'GGC
Cuts at: 2473 2473
Size: 4292
NarI GG'CG_CC
Cuts at: 1152 1723 1971 1152
Size: 571 248 3473
Fragments arranged by size:
3473 571 248
NgoMIV G'CCGG_C
Cuts at: 2471 2471
Size: 4292
NheI G'CTAG_C
Cuts at: 822 822
Size: 4292
NspI r_CATG'y
Cuts at: 1345 1845 2752 1345
Size: 500 907 2885
Fragments arranged by size:
2885 907 500
PasI CC'CwG_GG
Cuts at: 660 1011 660
Size: 351 3941
PciI A'CATG_T
Cuts at: 2748 2748
Size: 4292
PflMI CCAn_nnn'nTGG
Cuts at: 688 1101 688
Size: 413 3879
PfoI T'CCnGG_A
Cuts at: 1256 1256
Size: 4292
PmeI GTTT'AAAC
Cuts at: 1291 1291
Size: 4292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1108 1140 3431 3463 1108
Size: 32 2291 32 1937
Fragments arranged by size:
2291 1937 32 32
PpuMI rG'GwC_Cy
Cuts at: 367 367
Size: 4292
PspOMI G'GGCC_C
Cuts at: 388 636 388
Size: 248 4044
PstI C_TGCA'G
Cuts at: 284 1339 2024 284
Size: 1055 685 2552
Fragments arranged by size:
2552 1055 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4292
PvuII CAG'CTG
Cuts at: 1717 2077 1717
Size: 360 3932
RsrII CG'GwC_CG
Cuts at: 2487 2487
Size: 4292
SacI G_AGCT'C
Cuts at: 559 962 1265 1306 559
Size: 403 303 41 3545
Fragments arranged by size:
3545 403 303 41
SacII CC_GC'GG
Cuts at: 634 953 634
Size: 319 3973
SalI G'TCGA_C
Cuts at: 148 1329 148
Size: 1181 3111
SapI GCTCTTCn'nnn_
Cuts at: 301 2315 2525 301
Size: 2014 210 2068
Fragments arranged by size:
2068 2014 210
SbfI CC_TGCA'GG
Cuts at: 1339 1339
Size: 4292
ScaI AGT'ACT
Cuts at: 3809 3809
Size: 4292
SfcI C'TryA_G
Cuts at: 33 280 1335 2020 3013 3204 33
Size: 247 1055 685 993 191 1121
Fragments arranged by size:
1121 1055 993 685 247 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 844 844
Size: 4292
SfoI GGC'GCC
Cuts at: 1153 1724 1972 1153
Size: 571 248 3473
Fragments arranged by size:
3473 571 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4292
SmaI CCC'GGG
Cuts at: 372 794 1314 372
Size: 422 520 3350
Fragments arranged by size:
3350 520 422
SmlI C'TyrA_G
Cuts at: 1142 1447 2362 2854 3116 1142
Size: 305 915 492 262 2318
Fragments arranged by size:
2318 915 492 305 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4292
SphI G_CATG'C
Cuts at: 1345 1845 1345
Size: 500 3792
StuI AGG'CCT
Cuts at: 712 712
Size: 4292
StyI C'CwwG_G
Cuts at: 725 1426 1780 725
Size: 701 354 3237
Fragments arranged by size:
3237 701 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1875 2008 2467 2676 1875
Size: 133 459 209 3491
Fragments arranged by size:
3491 459 209 133
TatI w'GTAC_w
Cuts at: 3807 3807
Size: 4292
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 925 957 3809 3841 925
Size: 32 2852 32 1376
Fragments arranged by size:
2852 1376 32 32
Tth111I GACn'n_nGTC
Cuts at: 2089 2089
Size: 4292
XbaI T'CTAG_A
Cuts at: 1323 1323
Size: 4292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1787 1787
Size: 4292
XhoI C'TCGA_G
Cuts at: 1142 1142
Size: 4292
XmaI C'CCGG_G
Cuts at: 370 792 1312 370
Size: 422 520 3350
Fragments arranged by size:
3350 520 422
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NheI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI Bpu10I BsgI
BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NotI NruI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SanDI SexAI SgrAI SnaBI SrfI
SspI SwaI XmnI ZraI