Restriction map for
pF1KB8388
(Circular) (Six-base) MAPSORT of: pF1KB8388.seq Check: 1914 from: 1 to: 4859
pF1KB8388 4859 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 522 522
Size: 4859
AatII G_ACGT'C
Cuts at: 874 874
Size: 4859
AccI GT'mk_AC
Cuts at: 1897 1897
Size: 4859
Acc65I G'GTAC_C
Cuts at: 1875 1875
Size: 4859
AclI AA'CG_TT
Cuts at: 4699 4699
Size: 4859
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 740 856 940 1219 1439 2684 3116 3863
Size: 16 116 84 279 220 1245 432 747
Cuts at: 3863 4131 724
Size: 268 1452
Fragments arranged by size:
1452 1245 747 432 279 268 220 116
84 16
AflIII A'CryG_T
Cuts at: 2211 3315 4292 2211
Size: 1104 977 2778
Fragments arranged by size:
2778 1104 977
AgeI A'CCGG_T
Cuts at: 3247 3247
Size: 4859
AlwNI CAG_nnn'CTG
Cuts at: 447 918 993 1374 2164 3731 447
Size: 471 75 381 790 1567 1575
Fragments arranged by size:
1575 1567 790 471 381 75
ApaI G_GGCC'C
Cuts at: 810 1162 1718 810
Size: 352 556 3951
Fragments arranged by size:
3951 556 352
ApaLI G'TGCA_C
Cuts at: 1585 1762 3629 1585
Size: 177 1867 2815
Fragments arranged by size:
2815 1867 177
ApoI r'AATT_y
Cuts at: 1863 4212 4468 1863
Size: 2349 256 2254
Fragments arranged by size:
2349 2254 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4859
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4859
AvaI C'yCGr_G
Cuts at: 754 1072 1879 754
Size: 318 807 3734
Fragments arranged by size:
3734 807 318
BamHI G'GATC_C
Cuts at: 1884 1884
Size: 4859
BanI G'GyrC_C
Cuts at: 291 330 456 481 534 711 1377 1551 1875
Size: 39 126 25 53 177 666 174 324
Cuts at: 1875 2289 2537 2572 291
Size: 414 248 35 2578
Fragments arranged by size:
2578 666 414 324 248 177 174 126
53 39 35 25
BanII G_rGCy'C
Cuts at: 810 1004 1162 1624 1718 1755 1873 2903 810
Size: 194 158 462 94 37 118 1030 2766
Fragments arranged by size:
2766 1030 462 194 158 118 94 37
BbeI G_GCGC'C
Cuts at: 295 460 485 1555 2293 2541 295
Size: 165 25 1070 738 248 2613
Fragments arranged by size:
2613 1070 738 248 165 25
BbsI GAAGACnn'nnnn_
Cuts at: 874 1330 874
Size: 456 4403
BbvCI CC'TCA_GC
Cuts at: 401 659 401
Size: 258 4601
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1488 1522 1488
Size: 34 4825
BciVI GTATCCnnnnn_n'
Cuts at: 2757 3518 4859 2757
Size: 761 1341 2757
Fragments arranged by size:
2757 1341 761
BclI T'GATC_A
Cuts at: 2379 2379
Size: 4859
BglI GCCn_nnn'nGGC
Cuts at: 253 525 1525 1750 253
Size: 272 1000 225 3362
Fragments arranged by size:
3362 1000 272 225
BglII A'GATC_T
Cuts at: 2374 4411 2374
Size: 2037 2822
BlpI GC'TnA_GC
Cuts at: 1015 1171 1971 1015
Size: 156 800 3903
Fragments arranged by size:
3903 800 156
Bme1580I G_kGCm'C
Cuts at: 539 810 1162 1577 1589 1718 1766 2484 2577
Size: 271 352 415 12 129 48 718 93
Cuts at: 2577 3633 539
Size: 1056 1765
Fragments arranged by size:
1765 1056 718 415 352 271 129 93
48 12
BmrI ACTGGGnnnn_n'
Cuts at: 1825 2071 2152 2487 4631 1825
Size: 246 81 335 2144 2053
Fragments arranged by size:
2144 2053 335 246 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3226 3226
Size: 4859
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 668 1057 1370 148
Size: 520 389 313 3637
Fragments arranged by size:
3637 520 389 313
Bpu10I CC'TnA_GC
Cuts at: 401 659 1166 1754 401
Size: 258 507 588 3506
Fragments arranged by size:
3506 588 507 258
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 988 1828 2035 2914 3406 3704 988
Size: 840 207 879 492 298 2143
Fragments arranged by size:
2143 879 840 492 298 207
BsaI GGTCTCn'nnnn_
Cuts at: 823 4563 823
Size: 3740 1119
BsaAI yAC'GTr
Cuts at: 178 967 2212 178
Size: 789 1245 2825
Fragments arranged by size:
2825 1245 789
BsaBI GATnn'nnATC
Cuts at: 6 2373 4410 6
Size: 2367 2037 455
Fragments arranged by size:
2367 2037 455
BsaHI Gr'CG_yC
Cuts at: 292 457 482 871 1552 2290 2538 3240 4736
Size: 165 25 389 681 738 248 702 1496
Cuts at: 4736 292
Size: 415
Fragments arranged by size:
1496 738 702 681 415 389 248 165
25
BsaWI w'CCGG_w
Cuts at: 2048 2569 3247 3521 3668 4079 2048
Size: 521 678 274 147 411 2828
Fragments arranged by size:
2828 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 531 561 1072 1102 1252 1282 1606 1636 531
Size: 30 511 30 150 30 324 30 3754
Fragments arranged by size:
3754 511 324 150 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 196 1153 1334 196
Size: 957 181 3721
Fragments arranged by size:
3721 957 181
BseYI C'CCAG_C
Cuts at: 440 520 671 693 1184 1219 1408 1531 1625
Size: 80 151 22 491 35 189 123 94
Cuts at: 1625 1772 2284 3042 3619 4115 440
Size: 147 512 758 577 496 1184
Fragments arranged by size:
1184 758 577 512 496 491 189 151
147 123 94 80 35 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1321 1321
Size: 4859
BsiEI CG_ry'CG
Cuts at: 104 1706 1745 2401 2447 3655 104
Size: 1602 39 656 46 1208 1308
Fragments arranged by size:
1602 1308 1208 656 46 39
BsiHKAI G_wGCw'C
Cuts at: 177 1004 1589 1766 1796 1873 2651 2841 3633
Size: 827 585 177 30 77 778 190 792
Cuts at: 3633 177
Size: 1403
Fragments arranged by size:
1403 827 792 778 585 190 177 77
30
BsmBI CGTCTCn'nnnn_
Cuts at: 112 112
Size: 4859
Bsp1286I G_dGCh'C
Cuts at: 177 539 810 1004 1162 1577 1589 1624 1718
Size: 362 271 194 158 415 12 35 94
Cuts at: 1718 1755 1766 1796 1873 2484 2577 2651 2841
Size: 37 11 30 77 611 93 74 190
Cuts at: 2841 2903 3633 177
Size: 62 730 1403
Fragments arranged by size:
1403 730 611 415 362 271 194 190
158 94 93 77 74 62 37 35
30 12 11
BspEI T'CCGG_A
Cuts at: 4079 4079
Size: 4859
BspHI T'CATG_A
Cuts at: 4035 4035
Size: 4859
BspMI ACCTGCnnnn'nnnn_
Cuts at: 522 1909 2123 2425 2806 522
Size: 1387 214 302 381 2575
Fragments arranged by size:
2575 1387 381 302 214
BsrBI CCG'CTC
Cuts at: 50 192 957 3151 3205 4689 4854 50
Size: 142 765 2194 54 1484 165 55
Fragments arranged by size:
2194 1484 765 165 142 55 54
BsrDI GCAATG_nn'
Cuts at: 2771 2771
Size: 4859
BsrFI r'CCGG_y
Cuts at: 315 327 468 1353 1705 1740 2857 3038 3247
Size: 12 141 885 352 35 1117 181 209
Cuts at: 3247 315
Size: 1927
Fragments arranged by size:
1927 1117 885 352 209 181 141 35
12
BssSI C'ACGA_G
Cuts at: 1009 3130 3488 1009
Size: 2121 358 2380
Fragments arranged by size:
2380 2121 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2236 2236
Size: 4859
BstBI TT'CG_AA
Cuts at: 3220 3220
Size: 4859
BstEII G'GTnAC_C
Cuts at: 387 1812 387
Size: 1425 3434
BstXI CCAn_nnnn'nTGG
Cuts at: 243 243
Size: 4859
BstYI r'GATC_y
Cuts at: 1 1884 2351 2374 2709 2955 3967 4053 4065
Size: 1883 467 23 335 246 1012 86 12
Cuts at: 4065 4411 1
Size: 346 449
Fragments arranged by size:
1883 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 431 1271 1421 1649 1745 1808 431
Size: 840 150 228 96 63 3482
Fragments arranged by size:
3482 840 228 150 96 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1413 1491 1500 1650 2809 2990 3153 1413
Size: 78 9 150 1159 181 163 3119
Fragments arranged by size:
3119 1159 181 163 150 78 9
BtsI GCAGTG_nn'
Cuts at: 1661 2102 1661
Size: 441 4418
DraI TTT'AAA
Cuts at: 1858 1858
Size: 4859
DraIII CAC_nnn'GTG
Cuts at: 1272 1272
Size: 4859
DrdI GACnn_nn'nnGTC
Cuts at: 2565 3423 2565
Size: 858 4001
EaeI y'GGCC_r
Cuts at: 245 1703 1742 2444 2618 3009 3036 4381 245
Size: 1458 39 702 174 391 27 1345 723
Fragments arranged by size:
1458 1345 723 702 391 174 39 27
EagI C'GGCC_G
Cuts at: 1703 1742 2444 1703
Size: 39 702 4118
Fragments arranged by size:
4118 702 39
EarI CTCTTCn'nnn_
Cuts at: 742 916 2882 3092 742
Size: 174 1966 210 2509
Fragments arranged by size:
2509 1966 210 174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3067 3377 3523 4669 3067
Size: 310 146 1146 3257
Fragments arranged by size:
3257 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1002 1871 1002
Size: 869 3990
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 668 724 740 856 940 1057 1219 1370
Size: 520 56 16 116 84 117 162 151
Cuts at: 1370 1439 2684 3116 3863 4131 148
Size: 69 1245 432 747 268 876
Fragments arranged by size:
1245 876 747 520 432 268 162 151
117 116 84 69 56 16
EcoO109I rG'GnC_Cy
Cuts at: 698 806 1714 1715 1998 698
Size: 108 908 1 283 3559
Fragments arranged by size:
3559 908 283 108 1
EcoRI G'AATT_C
Cuts at: 1863 1863
Size: 4859
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2878 2878
Size: 4859
FseI GG_CCGG'CC
Cuts at: 1709 1709
Size: 4859
FspI TGC'GCA
Cuts at: 2640 2640
Size: 4859
HaeII r_GCGC'y
Cuts at: 295 460 485 640 901 1555 2293 2541 3563
Size: 165 25 155 261 654 738 248 1022
Cuts at: 3563 4542 295
Size: 979 612
Fragments arranged by size:
1022 979 738 654 612 261 248 165
155 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 795 827 861 893 1072 1104 1164 1196 2701
Size: 32 34 32 179 32 60 32 1505
Cuts at: 2701 2731 2733 2763 795
Size: 30 2 30 2891
Fragments arranged by size:
2891 1505 179 60 34 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1695 1898 1695
Size: 203 4656
KasI G'GCGC_C
Cuts at: 291 456 481 1551 2289 2537 291
Size: 165 25 1070 738 248 2613
Fragments arranged by size:
2613 1070 738 248 165 25
KpnI G_GTAC'C
Cuts at: 1879 1879
Size: 4859
MluI A'CGCG_T
Cuts at: 4292 4292
Size: 4859
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3530 3714 3530
Size: 184 4675
MscI TGG'CCA
Cuts at: 247 2620 247
Size: 2373 2486
MslI CAynn'nnrTG
Cuts at: 1582 2848 2975 1582
Size: 1266 127 3466
Fragments arranged by size:
3466 1266 127
MspA1I CmG'CkG
Cuts at: 433 663 718 990 1629 1776 1970 2284 2644
Size: 230 55 272 639 147 194 314 360
Cuts at: 2644 3657 3902 433
Size: 1013 245 1390
Fragments arranged by size:
1390 1013 639 360 314 272 245 230
194 147 55
NaeI GCC'GGC
Cuts at: 329 470 1707 1742 3040 329
Size: 141 1237 35 1298 2148
Fragments arranged by size:
2148 1298 1237 141 35
NarI GG'CG_CC
Cuts at: 292 457 482 1552 2290 2538 292
Size: 165 25 1070 738 248 2613
Fragments arranged by size:
2613 1070 738 248 165 25
NcoI C'CATG_G
Cuts at: 1808 1808
Size: 4859
NgoMIV G'CCGG_C
Cuts at: 327 468 1705 1740 3038 327
Size: 141 1237 35 1298 2148
Fragments arranged by size:
2148 1298 1237 141 35
NspI r_CATG'y
Cuts at: 1912 2412 3319 1912
Size: 500 907 3452
Fragments arranged by size:
3452 907 500
PciI A'CATG_T
Cuts at: 3315 3315
Size: 4859
PflMI CCAn_nnn'nTGG
Cuts at: 586 646 1026 1272 1374 1398 1779 586
Size: 60 380 246 102 24 381 3666
Fragments arranged by size:
3666 381 380 246 102 60 24
PfoI T'CCnGG_A
Cuts at: 143 143
Size: 4859
PmeI GTTT'AAAC
Cuts at: 1858 1858
Size: 4859
PmlI CAC'GTG
Cuts at: 178 967 178
Size: 789 4070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3998 4030 3998
Size: 32 4827
PpuMI rG'GwC_Cy
Cuts at: 698 698
Size: 4859
PshAI GACnn'nnGTC
Cuts at: 1810 1810
Size: 4859
PspOMI G'GGCC_C
Cuts at: 806 1158 1714 806
Size: 352 556 3951
Fragments arranged by size:
3951 556 352
PstI C_TGCA'G
Cuts at: 1487 1906 2591 1487
Size: 419 685 3755
Fragments arranged by size:
3755 685 419
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4859
PvuII CAG'CTG
Cuts at: 663 718 1629 1776 2284 2644 663
Size: 55 911 147 508 360 2878
Fragments arranged by size:
2878 911 508 360 147 55
RsrII CG'GwC_CG
Cuts at: 3054 3054
Size: 4859
SacI G_AGCT'C
Cuts at: 1004 1873 1004
Size: 869 3990
SacII CC_GC'GG
Cuts at: 434 434
Size: 4859
SalI G'TCGA_C
Cuts at: 1896 1896
Size: 4859
SanDI GG'GwC_CC
Cuts at: 698 698
Size: 4859
SapI GCTCTTCn'nnn_
Cuts at: 2882 3092 2882
Size: 210 4649
SbfI CC_TGCA'GG
Cuts at: 1906 1906
Size: 4859
ScaI AGT'ACT
Cuts at: 4376 4376
Size: 4859
SfcI C'TryA_G
Cuts at: 33 1483 1902 2587 3580 3771 33
Size: 1450 419 685 993 191 1121
Fragments arranged by size:
1450 1121 993 685 419 191
SfoI GGC'GCC
Cuts at: 293 458 483 1553 2291 2539 293
Size: 165 25 1070 738 248 2613
Fragments arranged by size:
2613 1070 738 248 165 25
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4859
SmaI CCC'GGG
Cuts at: 756 1881 756
Size: 1125 3734
SmlI C'TyrA_G
Cuts at: 1003 1843 2014 2929 3421 3683 1003
Size: 840 171 915 492 262 2179
Fragments arranged by size:
2179 915 840 492 262 171
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4859
SphI G_CATG'C
Cuts at: 1912 2412 1912
Size: 500 4359
StuI AGG'CCT
Cuts at: 1087 1087
Size: 4859
StyI C'CwwG_G
Cuts at: 1808 1993 2347 1808
Size: 185 354 4320
Fragments arranged by size:
4320 354 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 597 702 1265 2442 2575 3034 3243 597
Size: 105 563 1177 133 459 209 2213
Fragments arranged by size:
2213 1177 563 459 209 133 105
TatI w'GTAC_w
Cuts at: 4374 4374
Size: 4859
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 531 563 1252 1284 1606 1638 4376 4408 531
Size: 32 689 32 322 32 2738 32 982
Fragments arranged by size:
2738 982 689 322 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2656 2656
Size: 4859
XbaI T'CTAG_A
Cuts at: 1890 1890
Size: 4859
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2354 2354
Size: 4859
XmaI C'CCGG_G
Cuts at: 754 1879 754
Size: 1125 3734
ZraI GAC'GTC
Cuts at: 872 872
Size: 4859
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BfrBI BmgBI BmtI BsiWI BsmI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI