Restriction map for
pF1KB8385
(Circular) (Six-base) MAPSORT of: pF1KB8385.seq Check: 1436 from: 1 to: 4382
pF1KB8385 4382 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 421 1011 421
Size: 590 3792
AccI GT'mk_AC
Cuts at: 1049 1420 1049
Size: 371 4011
Acc65I G'GTAC_C
Cuts at: 1398 1398
Size: 4382
AclI AA'CG_TT
Cuts at: 4222 4222
Size: 4382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 774 2207 2639 3386 3654 774
Size: 1433 432 747 268 1502
Fragments arranged by size:
1502 1433 747 432 268
AflIII A'CryG_T
Cuts at: 1044 1358 1734 2838 3815 1044
Size: 314 376 1104 977 1611
Fragments arranged by size:
1611 1104 977 376 314
AgeI A'CCGG_T
Cuts at: 2770 2770
Size: 4382
AhdI GACnn_n'nnGTC
Cuts at: 852 852
Size: 4382
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1234 1234
Size: 4382
AlwNI CAG_nnn'CTG
Cuts at: 488 1687 3254 488
Size: 1199 1567 1616
Fragments arranged by size:
1616 1567 1199
ApaI G_GGCC'C
Cuts at: 328 810 328
Size: 482 3900
ApaLI G'TGCA_C
Cuts at: 3152 3152
Size: 4382
ApoI r'AATT_y
Cuts at: 1386 3735 3991 1386
Size: 2349 256 1777
Fragments arranged by size:
2349 1777 256
AscI GG'CGCG_CC
Cuts at: 248 248
Size: 4382
AseI AT'TA_AT
Cuts at: 20 20
Size: 4382
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
AvaI C'yCGr_G
Cuts at: 243 270 1402 243
Size: 27 1132 3223
Fragments arranged by size:
3223 1132 27
BamHI G'GATC_C
Cuts at: 1407 1407
Size: 4382
BanI G'GyrC_C
Cuts at: 214 927 1398 1812 2060 2095 214
Size: 713 471 414 248 35 2501
Fragments arranged by size:
2501 713 471 414 248 35
BanII G_rGCy'C
Cuts at: 244 328 698 810 1396 2426 244
Size: 84 370 112 586 1030 2200
Fragments arranged by size:
2200 1030 586 370 112 84
BbeI G_GCGC'C
Cuts at: 218 931 1816 2064 218
Size: 713 885 248 2536
Fragments arranged by size:
2536 885 713 248
BciVI GTATCCnnnnn_n'
Cuts at: 2280 3041 4382 2280
Size: 761 1341 2280
Fragments arranged by size:
2280 1341 761
BclI T'GATC_A
Cuts at: 1902 1902
Size: 4382
BglI GCCn_nnn'nGGC
Cuts at: 1208 1208
Size: 4382
BglII A'GATC_T
Cuts at: 1897 3934 1897
Size: 2037 2345
BlpI GC'TnA_GC
Cuts at: 1494 1494
Size: 4382
Bme1580I G_kGCm'C
Cuts at: 328 810 2007 2100 3156 328
Size: 482 1197 93 1056 1554
Fragments arranged by size:
1554 1197 1056 482 93
BmgBI CAC'GTC
Cuts at: 150 707 1047 1094 150
Size: 557 340 47 3438
Fragments arranged by size:
3438 557 340 47
BmrI ACTGGGnnnn_n'
Cuts at: 897 1594 1675 2010 4154 897
Size: 697 81 335 2144 1125
Fragments arranged by size:
2144 1125 697 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2749 2749
Size: 4382
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 894 1183 894
Size: 289 4093
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 1018 1558 2437 2929 3227 520
Size: 498 540 879 492 298 1675
Fragments arranged by size:
1675 879 540 498 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4086 4086
Size: 4382
BsaAI yAC'GTr
Cuts at: 924 1173 1735 924
Size: 249 562 3571
Fragments arranged by size:
3571 562 249
BsaBI GATnn'nnATC
Cuts at: 6 1896 3933 6
Size: 1890 2037 455
Fragments arranged by size:
2037 1890 455
BsaHI Gr'CG_yC
Cuts at: 215 928 1100 1813 2061 2763 4259 215
Size: 713 172 713 248 702 1496 338
Fragments arranged by size:
1496 713 713 702 338 248 172
BsaWI w'CCGG_w
Cuts at: 1571 2092 2770 3044 3191 3602 1571
Size: 521 678 274 147 411 2351
Fragments arranged by size:
2351 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 456 1180 1191 1242 456
Size: 724 11 51 3596
Fragments arranged by size:
3596 724 51 11
BseYI C'CCAG_C
Cuts at: 599 1198 1807 2565 3142 3638 599
Size: 599 609 758 577 496 1343
Fragments arranged by size:
1343 758 609 599 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 904 904
Size: 4382
BsiEI CG_ry'CG
Cuts at: 104 978 1924 1970 3178 104
Size: 874 946 46 1208 1308
Fragments arranged by size:
1308 1208 946 874 46
BsiHKAI G_wGCw'C
Cuts at: 893 910 1375 1396 2174 2364 3156 893
Size: 17 465 21 778 190 792 2119
Fragments arranged by size:
2119 792 778 465 190 21 17
BsmI GAATG_Cn'
Cuts at: 593 593
Size: 4382
Bsp1286I G_dGCh'C
Cuts at: 244 328 698 810 893 910 1375 1396 2007
Size: 84 370 112 83 17 465 21 611
Cuts at: 2007 2100 2174 2364 2426 3156 244
Size: 93 74 190 62 730 1470
Fragments arranged by size:
1470 730 611 465 370 190 112 93
84 83 74 62 21 17
BspEI T'CCGG_A
Cuts at: 3602 3602
Size: 4382
BspHI T'CATG_A
Cuts at: 3558 3558
Size: 4382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 421 1011 1432 1646 1948 2329 421
Size: 590 421 214 302 381 2474
Fragments arranged by size:
2474 590 421 381 302 214
BsrBI CCG'CTC
Cuts at: 50 389 2674 2728 4212 4377 50
Size: 339 2285 54 1484 165 55
Fragments arranged by size:
2285 1484 339 165 55 54
BsrDI GCAATG_nn'
Cuts at: 119 2294 119
Size: 2175 2207
BsrFI r'CCGG_y
Cuts at: 217 946 2380 2561 2770 217
Size: 729 1434 181 209 1829
Fragments arranged by size:
1829 1434 729 209 181
BssHII G'CGCG_C
Cuts at: 248 687 778 248
Size: 439 91 3852
Fragments arranged by size:
3852 439 91
BssSI C'ACGA_G
Cuts at: 2653 3011 2653
Size: 358 4024
BstAPI GCAn_nnn'nTGC
Cuts at: 518 725 1759 518
Size: 207 1034 3141
Fragments arranged by size:
3141 1034 207
BstBI TT'CG_AA
Cuts at: 2743 2743
Size: 4382
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4382
BstYI r'GATC_y
Cuts at: 1 135 831 1407 1874 1897 2232 2478 3490
Size: 134 696 576 467 23 335 246 1012
Cuts at: 3490 3576 3588 3934 1
Size: 86 12 346 449
Fragments arranged by size:
1012 696 576 467 449 346 335 246
134 86 23 12
BtgI C'CryG_G
Cuts at: 107 198 107
Size: 91 4291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2332 2513 2676 2332
Size: 181 163 4038
Fragments arranged by size:
4038 181 163
BtsI GCAGTG_nn'
Cuts at: 651 1159 1625 651
Size: 508 466 3408
Fragments arranged by size:
3408 508 466
DraI TTT'AAA
Cuts at: 1381 1381
Size: 4382
DrdI GACnn_nn'nnGTC
Cuts at: 2088 2946 2088
Size: 858 3524
EaeI y'GGCC_r
Cuts at: 152 402 648 773 938 1967 2141 2532 2559
Size: 250 246 125 165 1029 174 391 27
Cuts at: 2559 3904 152
Size: 1345 630
Fragments arranged by size:
1345 1029 630 391 250 246 174 165
125 27
EagI C'GGCC_G
Cuts at: 1967 1967
Size: 4382
EarI CTCTTCn'nnn_
Cuts at: 793 1276 2405 2615 793
Size: 483 1129 210 2560
Fragments arranged by size:
2560 1129 483 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2590 2900 3046 4192 2590
Size: 310 146 1146 2780
Fragments arranged by size:
2780 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1394 1394
Size: 4382
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 774 894 1183 2207 2639 3386 3654 774
Size: 120 289 1024 432 747 268 1502
Fragments arranged by size:
1502 1024 747 432 289 268 120
EcoO109I rG'GnC_Cy
Cuts at: 324 636 1521 324
Size: 312 885 3185
Fragments arranged by size:
3185 885 312
EcoRI G'AATT_C
Cuts at: 1386 1386
Size: 4382
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 4382
FspI TGC'GCA
Cuts at: 1158 1240 2163 1158
Size: 82 923 3377
Fragments arranged by size:
3377 923 82
FspAI rTGC'GCAy
Cuts at: 1158 1158
Size: 4382
HaeII r_GCGC'y
Cuts at: 218 289 797 931 1042 1816 2064 3086 4065
Size: 71 508 134 111 774 248 1022 979
Cuts at: 4065 218
Size: 535
Fragments arranged by size:
1022 979 774 535 508 248 134 111
71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 838 870 2224 2254 2256 2286 838
Size: 32 1354 30 2 30 2934
Fragments arranged by size:
2934 1354 32 30 30 2
HincII GTy'rAC
Cuts at: 1050 1421 1050
Size: 371 4011
KasI G'GCGC_C
Cuts at: 214 927 1812 2060 214
Size: 713 885 248 2536
Fragments arranged by size:
2536 885 713 248
KpnI G_GTAC'C
Cuts at: 1402 1402
Size: 4382
MluI A'CGCG_T
Cuts at: 3815 3815
Size: 4382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 619 1094 3053 3237 619
Size: 475 1959 184 1764
Fragments arranged by size:
1959 1764 475 184
MscI TGG'CCA
Cuts at: 2143 2143
Size: 4382
MslI CAynn'nnrTG
Cuts at: 112 2371 2498 112
Size: 2259 127 1996
Fragments arranged by size:
2259 1996 127
MspA1I CmG'CkG
Cuts at: 128 200 918 1067 1493 1807 2167 3180 3425
Size: 72 718 149 426 314 360 1013 245
Cuts at: 3425 128
Size: 1085
Fragments arranged by size:
1085 1013 718 426 360 314 245 149
72
NaeI GCC'GGC
Cuts at: 219 948 2563 219
Size: 729 1615 2038
Fragments arranged by size:
2038 1615 729
NarI GG'CG_CC
Cuts at: 215 928 1813 2061 215
Size: 713 885 248 2536
Fragments arranged by size:
2536 885 713 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4382
NgoMIV G'CCGG_C
Cuts at: 217 946 2561 217
Size: 729 1615 2038
Fragments arranged by size:
2038 1615 729
NspI r_CATG'y
Cuts at: 473 732 958 1362 1435 1935 2842 473
Size: 259 226 404 73 500 907 2013
Fragments arranged by size:
2013 907 500 404 259 226 73
PciI A'CATG_T
Cuts at: 1358 2838 1358
Size: 1480 2902
PfoI T'CCnGG_A
Cuts at: 827 1013 827
Size: 186 4196
PmeI GTTT'AAAC
Cuts at: 1381 1381
Size: 4382
PmlI CAC'GTG
Cuts at: 924 924
Size: 4382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3521 3553 3521
Size: 32 4350
PpuMI rG'GwC_Cy
Cuts at: 636 636
Size: 4382
PspOMI G'GGCC_C
Cuts at: 324 806 324
Size: 482 3900
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 448 480 448
Size: 32 4350
PstI C_TGCA'G
Cuts at: 839 1142 1429 2114 839
Size: 303 287 685 3107
Fragments arranged by size:
3107 685 303 287
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4382
PvuII CAG'CTG
Cuts at: 128 1067 1807 2167 128
Size: 939 740 360 2343
Fragments arranged by size:
2343 939 740 360
RsrII CG'GwC_CG
Cuts at: 2577 2577
Size: 4382
SacI G_AGCT'C
Cuts at: 1396 1396
Size: 4382
SacII CC_GC'GG
Cuts at: 201 201
Size: 4382
SalI G'TCGA_C
Cuts at: 1048 1419 1048
Size: 371 4011
SanDI GG'GwC_CC
Cuts at: 636 636
Size: 4382
SapI GCTCTTCn'nnn_
Cuts at: 793 1276 2405 2615 793
Size: 483 1129 210 2560
Fragments arranged by size:
2560 1129 483 210
SbfI CC_TGCA'GG
Cuts at: 1142 1429 1142
Size: 287 4095
ScaI AGT'ACT
Cuts at: 3899 3899
Size: 4382
SexAI A'CCwGG_T
Cuts at: 848 848
Size: 4382
SfcI C'TryA_G
Cuts at: 33 835 1138 1425 2110 3103 3294 33
Size: 802 303 287 685 993 191 1121
Fragments arranged by size:
1121 993 802 685 303 287 191
SfoI GGC'GCC
Cuts at: 216 929 1814 2062 216
Size: 713 885 248 2536
Fragments arranged by size:
2536 885 713 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
SgrAI Cr'CCGG_yG
Cuts at: 217 217
Size: 4382
SmaI CCC'GGG
Cuts at: 272 1404 272
Size: 1132 3250
SmlI C'TyrA_G
Cuts at: 535 1033 1537 2452 2944 3206 535
Size: 498 504 915 492 262 1711
Fragments arranged by size:
1711 915 504 498 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4382
SphI G_CATG'C
Cuts at: 732 958 1435 1935 732
Size: 226 477 500 3179
Fragments arranged by size:
3179 500 477 226
StyI C'CwwG_G
Cuts at: 107 1516 1870 107
Size: 1409 354 2619
Fragments arranged by size:
2619 1409 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1965 2098 2557 2766 1965
Size: 133 459 209 3581
Fragments arranged by size:
3581 459 209 133
TatI w'GTAC_w
Cuts at: 1061 3897 1061
Size: 2836 1546
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3899 3931 3899
Size: 32 4350
Tth111I GACn'n_nGTC
Cuts at: 979 2179 979
Size: 1200 3182
XbaI T'CTAG_A
Cuts at: 1413 1413
Size: 4382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 744 1877 744
Size: 1133 3249
XmaI C'CCGG_G
Cuts at: 270 1402 270
Size: 1132 3250
XmnI GAAnn'nnTTC
Cuts at: 572 572
Size: 4382
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI BamHI BanI BanII BbeI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PmlI PpiI PpuMI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI AvrII BaeI BbsI
BbvCI BcgI BfrBI BmtI Bpu10I BsaXI BsiWI BsmBI
BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PshAI PsiI PspXI
SfiI SnaBI SrfI SspI StuI SwaI XhoI ZraI