Restriction map for
pF1KB8383
(Circular) (Six-base) MAPSORT of: pF1KB8383.seq Check: 9759 from: 1 to: 4850
pF1KB8383 4850 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1888 1888
Size: 4850
Acc65I G'GTAC_C
Cuts at: 1866 1866
Size: 4850
AclI AA'CG_TT
Cuts at: 4690 4690
Size: 4850
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2675 3107 3854 4122 2675
Size: 432 747 268 3403
Fragments arranged by size:
3403 747 432 268
AflIII A'CryG_T
Cuts at: 368 2202 3306 4283 368
Size: 1834 1104 977 935
Fragments arranged by size:
1834 1104 977 935
AgeI A'CCGG_T
Cuts at: 3238 3238
Size: 4850
AhdI GACnn_n'nnGTC
Cuts at: 1778 1778
Size: 4850
AleI CACnn'nnGTG
Cuts at: 1822 1822
Size: 4850
AlwNI CAG_nnn'CTG
Cuts at: 493 2155 3722 493
Size: 1662 1567 1621
Fragments arranged by size:
1662 1621 1567
ApaLI G'TGCA_C
Cuts at: 3620 3620
Size: 4850
ApoI r'AATT_y
Cuts at: 543 951 1811 1854 4203 4459 543
Size: 408 860 43 2349 256 934
Fragments arranged by size:
2349 934 860 408 256 43
AseI AT'TA_AT
Cuts at: 20 704 20
Size: 684 4166
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4850
AvaI C'yCGr_G
Cuts at: 1870 1870
Size: 4850
BamHI G'GATC_C
Cuts at: 1875 1875
Size: 4850
BanI G'GyrC_C
Cuts at: 1866 2280 2528 2563 1866
Size: 414 248 35 4153
Fragments arranged by size:
4153 414 248 35
BanII G_rGCy'C
Cuts at: 305 978 1227 1864 2894 305
Size: 673 249 637 1030 2261
Fragments arranged by size:
2261 1030 673 637 249
BbeI G_GCGC'C
Cuts at: 2284 2532 2284
Size: 248 4602
BbsI GAAGACnn'nnnn_
Cuts at: 1777 1777
Size: 4850
BciVI GTATCCnnnnn_n'
Cuts at: 2748 3509 4850 2748
Size: 761 1341 2748
Fragments arranged by size:
2748 1341 761
BclI T'GATC_A
Cuts at: 684 2370 684
Size: 1686 3164
BfrBI ATG'CAT
Cuts at: 1510 1762 1510
Size: 252 4598
BglII A'GATC_T
Cuts at: 808 2365 4402 808
Size: 1557 2037 1256
Fragments arranged by size:
2037 1557 1256
BlpI GC'TnA_GC
Cuts at: 1962 1962
Size: 4850
Bme1580I G_kGCm'C
Cuts at: 487 2475 2568 3624 487
Size: 1988 93 1056 1713
Fragments arranged by size:
1988 1713 1056 93
BmgBI CAC'GTC
Cuts at: 369 369
Size: 4850
BmrI ACTGGGnnnn_n'
Cuts at: 1661 2062 2143 2478 4622 1661
Size: 401 81 335 2144 1889
Fragments arranged by size:
2144 1889 401 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3217 3217
Size: 4850
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2026 2905 3397 3695 2026
Size: 879 492 298 3181
Fragments arranged by size:
3181 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4554 4554
Size: 4850
BsaAI yAC'GTr
Cuts at: 2203 2203
Size: 4850
BsaBI GATnn'nnATC
Cuts at: 6 2364 4401 6
Size: 2358 2037 455
Fragments arranged by size:
2358 2037 455
BsaHI Gr'CG_yC
Cuts at: 2281 2529 3231 4727 2281
Size: 248 702 1496 2404
Fragments arranged by size:
2404 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2039 2560 3238 3512 3659 4070 2039
Size: 521 678 274 147 411 2819
Fragments arranged by size:
2819 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 812 842 1564 1594 1732 1762 812
Size: 30 722 30 138 30 3900
Fragments arranged by size:
3900 722 138 30 30 30
BseYI C'CCAG_C
Cuts at: 1211 2275 3033 3610 4106 1211
Size: 1064 758 577 496 1955
Fragments arranged by size:
1955 1064 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2392 2438 3646 104
Size: 2288 46 1208 1308
Fragments arranged by size:
2288 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 978 1227 1796 1864 2642 2832 3624 978
Size: 249 569 68 778 190 792 2204
Fragments arranged by size:
2204 792 778 569 249 190 68
BsmI GAATG_Cn'
Cuts at: 702 702
Size: 4850
Bsp1286I G_dGCh'C
Cuts at: 305 487 978 1227 1796 1864 2475 2568 2642
Size: 182 491 249 569 68 611 93 74
Cuts at: 2642 2832 2894 3624 305
Size: 190 62 730 1531
Fragments arranged by size:
1531 730 611 569 491 249 190 182
93 74 68 62
BspEI T'CCGG_A
Cuts at: 4070 4070
Size: 4850
BspHI T'CATG_A
Cuts at: 4026 4026
Size: 4850
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1900 2114 2416 2797 1900
Size: 214 302 381 3953
Fragments arranged by size:
3953 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3142 3196 4680 4845 50
Size: 3092 54 1484 165 55
Fragments arranged by size:
3092 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1432 2762 1432
Size: 1330 3520
BsrFI r'CCGG_y
Cuts at: 2848 3029 3238 2848
Size: 181 209 4460
Fragments arranged by size:
4460 209 181
BsrGI T'GTAC_A
Cuts at: 1632 1632
Size: 4850
BssSI C'ACGA_G
Cuts at: 3121 3479 3121
Size: 358 4492
BstAPI GCAn_nnn'nTGC
Cuts at: 929 2227 929
Size: 1298 3552
BstBI TT'CG_AA
Cuts at: 3211 3211
Size: 4850
BstYI r'GATC_y
Cuts at: 1 717 808 1875 2342 2365 2700 2946 3958
Size: 716 91 1067 467 23 335 246 1012
Cuts at: 3958 4044 4056 4402 1
Size: 86 12 346 449
Fragments arranged by size:
1067 1012 716 467 449 346 335 246
91 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2800 2981 3144 2800
Size: 181 163 4506
Fragments arranged by size:
4506 181 163
BtsI GCAGTG_nn'
Cuts at: 2093 2093
Size: 4850
DraI TTT'AAA
Cuts at: 872 1849 872
Size: 977 3873
DrdI GACnn_nn'nnGTC
Cuts at: 2556 3414 2556
Size: 858 3992
EaeI y'GGCC_r
Cuts at: 738 2435 2609 3000 3027 4372 738
Size: 1697 174 391 27 1345 1216
Fragments arranged by size:
1697 1345 1216 391 174 27
EagI C'GGCC_G
Cuts at: 2435 2435
Size: 4850
EarI CTCTTCn'nnn_
Cuts at: 161 293 2873 3083 161
Size: 132 2580 210 1928
Fragments arranged by size:
2580 1928 210 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3058 3368 3514 4660 3058
Size: 310 146 1146 3248
Fragments arranged by size:
3248 1146 310 146
EcoICRI GAG'CTC
Cuts at: 976 1225 1862 976
Size: 249 637 3964
Fragments arranged by size:
3964 637 249
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2675 3107 3854 4122 2675
Size: 432 747 268 3403
Fragments arranged by size:
3403 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1989 1989
Size: 4850
EcoRI G'AATT_C
Cuts at: 1811 1854 1811
Size: 43 4807
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 488 2869 488
Size: 2381 2469
FspI TGC'GCA
Cuts at: 2631 2631
Size: 4850
HaeII r_GCGC'y
Cuts at: 2284 2532 3554 4533 2284
Size: 248 1022 979 2601
Fragments arranged by size:
2601 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 200 232 812 844 1368 1400 1526 1558 1764
Size: 32 580 32 524 32 126 32 206
Cuts at: 1764 1796 2692 2722 2724 2754 200
Size: 32 896 30 2 30 2296
Fragments arranged by size:
2296 896 580 524 206 126 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1889 1889
Size: 4850
KasI G'GCGC_C
Cuts at: 2280 2528 2280
Size: 248 4602
KpnI G_GTAC'C
Cuts at: 1870 1870
Size: 4850
MluI A'CGCG_T
Cuts at: 4283 4283
Size: 4850
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3521 3705 3521
Size: 184 4666
MscI TGG'CCA
Cuts at: 740 2611 740
Size: 1871 2979
MslI CAynn'nnrTG
Cuts at: 1668 1822 2839 2966 1668
Size: 154 1017 127 3552
Fragments arranged by size:
3552 1017 154 127
MspA1I CmG'CkG
Cuts at: 1961 2275 2635 3648 3893 1961
Size: 314 360 1013 245 2918
Fragments arranged by size:
2918 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3031 3031
Size: 4850
NarI GG'CG_CC
Cuts at: 2281 2529 2281
Size: 248 4602
NgoMIV G'CCGG_C
Cuts at: 3029 3029
Size: 4850
NsiI A_TGCA'T
Cuts at: 1512 1764 1512
Size: 252 4598
NspI r_CATG'y
Cuts at: 1514 1766 1903 2403 3310 1514
Size: 252 137 500 907 3054
Fragments arranged by size:
3054 907 500 252 137
PciI A'CATG_T
Cuts at: 3306 3306
Size: 4850
PflMI CCAn_nnn'nTGG
Cuts at: 736 736
Size: 4850
PfoI T'CCnGG_A
Cuts at: 218 218
Size: 4850
PmeI GTTT'AAAC
Cuts at: 1849 1849
Size: 4850
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3989 4021 3989
Size: 32 4818
PsiI TTA'TAA
Cuts at: 677 677
Size: 4850
PstI C_TGCA'G
Cuts at: 1897 2582 1897
Size: 685 4165
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4850
PvuII CAG'CTG
Cuts at: 2275 2635 2275
Size: 360 4490
RsrII CG'GwC_CG
Cuts at: 3045 3045
Size: 4850
SacI G_AGCT'C
Cuts at: 978 1227 1864 978
Size: 249 637 3964
Fragments arranged by size:
3964 637 249
SalI G'TCGA_C
Cuts at: 1887 1887
Size: 4850
SapI GCTCTTCn'nnn_
Cuts at: 293 2873 3083 293
Size: 2580 210 2060
Fragments arranged by size:
2580 2060 210
SbfI CC_TGCA'GG
Cuts at: 1897 1897
Size: 4850
ScaI AGT'ACT
Cuts at: 4367 4367
Size: 4850
SfcI C'TryA_G
Cuts at: 33 1893 2578 3571 3762 33
Size: 1860 685 993 191 1121
Fragments arranged by size:
1860 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2282 2530 2282
Size: 248 4602
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4850
SmaI CCC'GGG
Cuts at: 1872 1872
Size: 4850
SmlI C'TyrA_G
Cuts at: 2005 2920 3412 3674 2005
Size: 915 492 262 3181
Fragments arranged by size:
3181 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4850
SphI G_CATG'C
Cuts at: 1903 2403 1903
Size: 500 4350
SspI AAT'ATT
Cuts at: 555 555
Size: 4850
StyI C'CwwG_G
Cuts at: 1984 2338 1984
Size: 354 4496
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 356 2433 2566 3025 3234 356
Size: 2077 133 459 209 1972
Fragments arranged by size:
2077 1972 459 209 133
TatI w'GTAC_w
Cuts at: 1140 1336 1632 4365 1140
Size: 196 296 2733 1625
Fragments arranged by size:
2733 1625 296 196
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4367 4399 4367
Size: 32 4818
Tth111I GACn'n_nGTC
Cuts at: 2647 2647
Size: 4850
XbaI T'CTAG_A
Cuts at: 1881 1881
Size: 4850
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 350 2345 350
Size: 1995 2855
XmaI C'CCGG_G
Cuts at: 1870 1870
Size: 4850
XmnI GAAnn'nnTTC
Cuts at: 940 1270 1522 1690 940
Size: 330 252 168 4100
Fragments arranged by size:
4100 330 252 168
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmtI BpmI Bpu10I
BseRI BsgI BsiWI BsmBI BssHII BstEII BstXI BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI PacI PasI PmlI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI