Restriction map for
pF1KB8378
(Circular) (Six-base) MAPSORT of: pF1KB8378.seq Check: 5965 from: 1 to: 4787
pF1KB8378 4787 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 212 270 1686 212
Size: 58 1416 3313
Fragments arranged by size:
3313 1416 58
AccI GT'mk_AC
Cuts at: 1825 1825
Size: 4787
Acc65I G'GTAC_C
Cuts at: 1803 1803
Size: 4787
AclI AA'CG_TT
Cuts at: 4627 4627
Size: 4787
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 396 606 1144 2612 3044 3791 4059 396
Size: 210 538 1468 432 747 268 1124
Fragments arranged by size:
1468 1124 747 538 432 268 210
AflIII A'CryG_T
Cuts at: 1371 2139 3243 4220 1371
Size: 768 1104 977 1938
Fragments arranged by size:
1938 1104 977 768
AgeI A'CCGG_T
Cuts at: 1779 3175 1779
Size: 1396 3391
AleI CACnn'nnGTG
Cuts at: 1342 1342
Size: 4787
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1066 1098 1066
Size: 32 4755
AlwNI CAG_nnn'CTG
Cuts at: 372 1554 2092 3659 372
Size: 1182 538 1567 1500
Fragments arranged by size:
1567 1500 1182 538
ApaI G_GGCC'C
Cuts at: 404 404
Size: 4787
ApaLI G'TGCA_C
Cuts at: 450 3557 450
Size: 3107 1680
ApoI r'AATT_y
Cuts at: 838 1791 4140 4396 838
Size: 953 2349 256 1229
Fragments arranged by size:
2349 1229 953 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4787
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4787
AvaI C'yCGr_G
Cuts at: 145 558 812 1731 1807 145
Size: 413 254 919 76 3125
Fragments arranged by size:
3125 919 413 254 76
BamHI G'GATC_C
Cuts at: 1205 1812 1205
Size: 607 4180
BanI G'GyrC_C
Cuts at: 200 1347 1803 2217 2465 2500 200
Size: 1147 456 414 248 35 2487
Fragments arranged by size:
2487 1147 456 414 248 35
BanII G_rGCy'C
Cuts at: 121 404 584 650 1203 1598 1640 1801 2831
Size: 283 180 66 553 395 42 161 1030
Cuts at: 2831 121
Size: 2077
Fragments arranged by size:
2077 1030 553 395 283 180 161 66
42
BbeI G_GCGC'C
Cuts at: 1351 2221 2469 1351
Size: 870 248 3669
Fragments arranged by size:
3669 870 248
BbsI GAAGACnn'nnnn_
Cuts at: 327 327
Size: 4787
BciVI GTATCCnnnnn_n'
Cuts at: 2685 3446 4787 2685
Size: 761 1341 2685
Fragments arranged by size:
2685 1341 761
BclI T'GATC_A
Cuts at: 2307 2307
Size: 4787
BglI GCCn_nnn'nGGC
Cuts at: 1721 1759 1721
Size: 38 4749
BglII A'GATC_T
Cuts at: 1148 2302 4339 1148
Size: 1154 2037 1596
Fragments arranged by size:
2037 1596 1154
BlpI GC'TnA_GC
Cuts at: 190 1899 190
Size: 1709 3078
Bme1580I G_kGCm'C
Cuts at: 404 454 1628 2412 2505 3561 404
Size: 50 1174 784 93 1056 1630
Fragments arranged by size:
1630 1174 1056 784 93 50
BmrI ACTGGGnnnn_n'
Cuts at: 885 1999 2080 2415 4559 885
Size: 1114 81 335 2144 1113
Fragments arranged by size:
2144 1114 1113 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 573 3154 573
Size: 2581 2206
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 664
Size: 4787
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 955 1624 1963 2842 3334 3632 487
Size: 468 669 339 879 492 298 1642
Fragments arranged by size:
1642 879 669 492 468 339 298
BsaI GGTCTCn'nnnn_
Cuts at: 1058 4491 1058
Size: 3433 1354
BsaAI yAC'GTr
Cuts at: 576 2140 576
Size: 1564 3223
BsaBI GATnn'nnATC
Cuts at: 6 2301 4338 6
Size: 2295 2037 455
Fragments arranged by size:
2295 2037 455
BsaHI Gr'CG_yC
Cuts at: 1348 1571 2218 2466 3168 4664 1348
Size: 223 647 248 702 1496 1471
Fragments arranged by size:
1496 1471 702 647 248 223
BsaWI w'CCGG_w
Cuts at: 302 1779 1976 2497 3175 3449 3596 4007 302
Size: 1477 197 521 678 274 147 411 1082
Fragments arranged by size:
1477 1082 678 521 411 274 197 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 941 971 1066 1096 941
Size: 30 95 30 4632
Fragments arranged by size:
4632 95 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 373 784 871 892 1402 373
Size: 411 87 21 510 3758
Fragments arranged by size:
3758 510 411 87 21
BseYI C'CCAG_C
Cuts at: 238 403 2212 2970 3547 4043 238
Size: 165 1809 758 577 496 982
Fragments arranged by size:
1809 982 758 577 496 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 385 1072 1758 298
Size: 87 687 686 3327
Fragments arranged by size:
3327 687 686 87
BsiEI CG_ry'CG
Cuts at: 104 1385 2329 2375 3583 104
Size: 1281 944 46 1208 1308
Fragments arranged by size:
1308 1281 1208 944 46
BsiHKAI G_wGCw'C
Cuts at: 454 584 650 1396 1640 1801 2579 2769 3561
Size: 130 66 746 244 161 778 190 792
Cuts at: 3561 454
Size: 1680
Fragments arranged by size:
1680 792 778 746 244 190 161 130
66
BsmBI CGTCTCn'nnnn_
Cuts at: 921 921
Size: 4787
Bsp1286I G_dGCh'C
Cuts at: 121 404 454 584 650 1203 1396 1598 1628
Size: 283 50 130 66 553 193 202 30
Cuts at: 1628 1640 1801 2412 2505 2579 2769 2831 3561
Size: 12 161 611 93 74 190 62 730
Cuts at: 3561 121
Size: 1347
Fragments arranged by size:
1347 730 611 553 283 202 193 190
161 130 93 74 66 62 50 30
12
BspEI T'CCGG_A
Cuts at: 302 4007 302
Size: 3705 1082
BspHI T'CATG_A
Cuts at: 3963 3963
Size: 4787
BspMI ACCTGCnnnn'nnnn_
Cuts at: 212 270 1539 1686 1837 2051 2353 2734 212
Size: 58 1269 147 151 214 302 381 2265
Fragments arranged by size:
2265 1269 381 302 214 151 147 58
BsrBI CCG'CTC
Cuts at: 50 222 3079 3133 4617 4782 50
Size: 172 2857 54 1484 165 55
Fragments arranged by size:
2857 1484 172 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2699 2699
Size: 4787
BsrFI r'CCGG_y
Cuts at: 197 1779 2785 2966 3175 197
Size: 1582 1006 181 209 1809
Fragments arranged by size:
1809 1582 1006 209 181
BsrGI T'GTAC_A
Cuts at: 686 686
Size: 4787
BssSI C'ACGA_G
Cuts at: 3058 3416 3058
Size: 358 4429
BstAPI GCAn_nnn'nTGC
Cuts at: 372 449 1654 2164 372
Size: 77 1205 510 2995
Fragments arranged by size:
2995 1205 510 77
BstBI TT'CG_AA
Cuts at: 3148 3148
Size: 4787
BstEII G'GTnAC_C
Cuts at: 483 1446 483
Size: 963 3824
BstXI CCAn_nnnn'nTGG
Cuts at: 603 1344 603
Size: 741 4046
BstYI r'GATC_y
Cuts at: 1 1022 1148 1205 1812 2279 2302 2637 2883
Size: 1021 126 57 607 467 23 335 246
Cuts at: 2883 3895 3981 3993 4339 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1021 1012 607 467 449 346 335 246
126 86 57 23 12
Bsu36I CC'TnA_GG
Cuts at: 1256 1256
Size: 4787
BtgI C'CryG_G
Cuts at: 1034 1069 1139 1585 1034
Size: 35 70 446 4236
Fragments arranged by size:
4236 446 70 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2737 2918 3081 2737
Size: 181 163 4443
Fragments arranged by size:
4443 181 163
BtsI GCAGTG_nn'
Cuts at: 2030 2030
Size: 4787
DraI TTT'AAA
Cuts at: 1786 1786
Size: 4787
DraIII CAC_nnn'GTG
Cuts at: 984 1591 984
Size: 607 4180
DrdI GACnn_nn'nnGTC
Cuts at: 1549 1570 2493 3351 1549
Size: 21 923 858 2985
Fragments arranged by size:
2985 923 858 21
EaeI y'GGCC_r
Cuts at: 1037 1382 1457 1664 1679 2372 2546 2937 2964
Size: 345 75 207 15 693 174 391 27
Cuts at: 2964 4309 1037
Size: 1345 1515
Fragments arranged by size:
1515 1345 693 391 345 207 174 75
27 15
EagI C'GGCC_G
Cuts at: 1382 2372 1382
Size: 990 3797
EarI CTCTTCn'nnn_
Cuts at: 625 919 2810 3020 625
Size: 294 1891 210 2392
Fragments arranged by size:
2392 1891 294 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2995 3305 3451 4597 2995
Size: 310 146 1146 3185
Fragments arranged by size:
3185 1146 310 146
EcoICRI GAG'CTC
Cuts at: 582 648 1638 1799 582
Size: 66 990 161 3570
Fragments arranged by size:
3570 990 161 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 396 606 664 1144 2612 3044 3791 4059 396
Size: 210 58 480 1468 432 747 268 1124
Fragments arranged by size:
1468 1124 747 480 432 268 210 58
EcoO109I rG'GnC_Cy
Cuts at: 615 1551 1686 1926 615
Size: 936 135 240 3476
Fragments arranged by size:
3476 936 240 135
EcoRI G'AATT_C
Cuts at: 838 1791 838
Size: 953 3834
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 636 843 2806 636
Size: 207 1963 2617
Fragments arranged by size:
2617 1963 207
FspI TGC'GCA
Cuts at: 2568 2568
Size: 4787
HaeII r_GCGC'y
Cuts at: 1351 1663 2221 2469 3491 4470 1351
Size: 312 558 248 1022 979 1668
Fragments arranged by size:
1668 1022 979 558 312 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 765 793 797 825 941 973 1190 1222 2629
Size: 28 4 28 116 32 217 32 1407
Cuts at: 2629 2659 2661 2691 765
Size: 30 2 30 2861
Fragments arranged by size:
2861 1407 217 116 32 32 30 30
28 28 4 2
HincII GTy'rAC
Cuts at: 660 1826 660
Size: 1166 3621
KasI G'GCGC_C
Cuts at: 1347 2217 2465 1347
Size: 870 248 3669
Fragments arranged by size:
3669 870 248
KpnI G_GTAC'C
Cuts at: 1807 1807
Size: 4787
MluI A'CGCG_T
Cuts at: 1371 4220 1371
Size: 2849 1938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 257 656 3458 3642 257
Size: 399 2802 184 1402
Fragments arranged by size:
2802 1402 399 184
MscI TGG'CCA
Cuts at: 1039 1666 2548 1039
Size: 627 882 3278
Fragments arranged by size:
3278 882 627
MslI CAynn'nnrTG
Cuts at: 538 1342 1513 1543 2776 2903 538
Size: 804 171 30 1233 127 2422
Fragments arranged by size:
2422 1233 804 171 127 30
MspA1I CmG'CkG
Cuts at: 194 407 416 495 681 764 1071 1079 1898
Size: 213 9 79 186 83 307 8 819
Cuts at: 1898 2212 2572 3585 3830 194
Size: 314 360 1013 245 1151
Fragments arranged by size:
1151 1013 819 360 314 307 245 213
186 83 79 9 8
NaeI GCC'GGC
Cuts at: 199 2968 199
Size: 2769 2018
NarI GG'CG_CC
Cuts at: 1348 2218 2466 1348
Size: 870 248 3669
Fragments arranged by size:
3669 870 248
NgoMIV G'CCGG_C
Cuts at: 197 2966 197
Size: 2769 2018
NspI r_CATG'y
Cuts at: 1548 1840 2340 3247 1548
Size: 292 500 907 3088
Fragments arranged by size:
3088 907 500 292
PasI CC'CwG_GG
Cuts at: 456 1316 456
Size: 860 3927
PciI A'CATG_T
Cuts at: 3243 3243
Size: 4787
PflMI CCAn_nnn'nTGG
Cuts at: 1287 1287
Size: 4787
PfoI T'CCnGG_A
Cuts at: 641 995 1553 1604 1631 641
Size: 354 558 51 27 3797
Fragments arranged by size:
3797 558 354 51 27
PmeI GTTT'AAAC
Cuts at: 1786 1786
Size: 4787
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1066 1098 3926 3958 1066
Size: 32 2828 32 1895
Fragments arranged by size:
2828 1895 32 32
PpuMI rG'GwC_Cy
Cuts at: 615 1551 1686 615
Size: 936 135 3716
Fragments arranged by size:
3716 936 135
PsiI TTA'TAA
Cuts at: 938 938
Size: 4787
PspOMI G'GGCC_C
Cuts at: 400 400
Size: 4787
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 556 588 898 930 556
Size: 32 310 32 4413
Fragments arranged by size:
4413 310 32 32
PstI C_TGCA'G
Cuts at: 257 763 1834 2519 257
Size: 506 1071 685 2525
Fragments arranged by size:
2525 1071 685 506
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4787
PvuII CAG'CTG
Cuts at: 194 416 681 764 1079 2212 2572 194
Size: 222 265 83 315 1133 360 2409
Fragments arranged by size:
2409 1133 360 315 265 222 83
RsrII CG'GwC_CG
Cuts at: 2982 2982
Size: 4787
SacI G_AGCT'C
Cuts at: 584 650 1640 1801 584
Size: 66 990 161 3570
Fragments arranged by size:
3570 990 161 66
SacII CC_GC'GG
Cuts at: 1072 1072
Size: 4787
SalI G'TCGA_C
Cuts at: 1824 1824
Size: 4787
SapI GCTCTTCn'nnn_
Cuts at: 2810 3020 2810
Size: 210 4577
SbfI CC_TGCA'GG
Cuts at: 1834 1834
Size: 4787
ScaI AGT'ACT
Cuts at: 1708 4304 1708
Size: 2596 2191
SfcI C'TryA_G
Cuts at: 33 253 651 759 1710 1830 2515 3508 3699
Size: 220 398 108 951 120 685 993 191
Cuts at: 3699 33
Size: 1121
Fragments arranged by size:
1121 993 951 685 398 220 191 120
108
SfoI GGC'GCC
Cuts at: 1349 2219 2467 1349
Size: 870 248 3669
Fragments arranged by size:
3669 870 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4787
SmaI CCC'GGG
Cuts at: 147 1809 147
Size: 1662 3125
SmlI C'TyrA_G
Cuts at: 502 812 970 1639 1942 2857 3349 3611 502
Size: 310 158 669 303 915 492 262 1678
Fragments arranged by size:
1678 915 669 492 310 303 262 158
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4787
SphI G_CATG'C
Cuts at: 1840 2340 1840
Size: 500 4287
StuI AGG'CCT
Cuts at: 733 780 871 1297 1724 733
Size: 47 91 426 427 3796
Fragments arranged by size:
3796 427 426 91 47
StyI C'CwwG_G
Cuts at: 329 1361 1921 2275 329
Size: 1032 560 354 2841
Fragments arranged by size:
2841 1032 560 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 264 1465 1732 2370 2503 2962 3171 264
Size: 1201 267 638 133 459 209 1880
Fragments arranged by size:
1880 1201 638 459 267 209 133
TatI w'GTAC_w
Cuts at: 686 986 1706 4302 686
Size: 300 720 2596 1171
Fragments arranged by size:
2596 1171 720 300
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1252 1284 4304 4336 1252
Size: 32 3020 32 1703
Fragments arranged by size:
3020 1703 32 32
Tth111I GACn'n_nGTC
Cuts at: 1570 2584 1570
Size: 1014 3773
XbaI T'CTAG_A
Cuts at: 1818 1818
Size: 4787
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1512 1674 2282 1512
Size: 162 608 4017
Fragments arranged by size:
4017 608 162
XhoI C'TCGA_G
Cuts at: 812 812
Size: 4787
XmaI C'CCGG_G
Cuts at: 145 1807 145
Size: 1662 3125
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PsiI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI
BssHII BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PspXI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XmnI ZraI