Restriction map for
pF1KB8374
(Circular) (Six-base) MAPSORT of: pF1KB8374.seq Check: 2725 from: 1 to: 4997
pF1KB8374 4997 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AatII G_ACGT'C
Cuts at: 1412 1412
Size: 4997
AccI GT'mk_AC
Cuts at: 1448 2035 1448
Size: 587 4410
Acc65I G'GTAC_C
Cuts at: 2013 2013
Size: 4997
AclI AA'CG_TT
Cuts at: 4837 4837
Size: 4997
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 614 815 2822 3254 4001 4269 614
Size: 201 2007 432 747 268 1342
Fragments arranged by size:
2007 1342 747 432 268 201
AfeI AGC'GCT
Cuts at: 157 157
Size: 4997
AflIII A'CryG_T
Cuts at: 2349 3453 4430 2349
Size: 1104 977 2916
Fragments arranged by size:
2916 1104 977
AgeI A'CCGG_T
Cuts at: 3385 3385
Size: 4997
AleI CACnn'nnGTG
Cuts at: 520 520
Size: 4997
AlwNI CAG_nnn'CTG
Cuts at: 1213 2302 3869 1213
Size: 1089 1567 2341
Fragments arranged by size:
2341 1567 1089
ApaI G_GGCC'C
Cuts at: 1549 1818 1549
Size: 269 4728
ApaLI G'TGCA_C
Cuts at: 3767 3767
Size: 4997
ApoI r'AATT_y
Cuts at: 742 1937 2001 4350 4606 742
Size: 1195 64 2349 256 1133
Fragments arranged by size:
2349 1195 1133 256 64
AseI AT'TA_AT
Cuts at: 20 20
Size: 4997
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4997
AvaI C'yCGr_G
Cuts at: 1140 2017 1140
Size: 877 4120
BamHI G'GATC_C
Cuts at: 2022 2022
Size: 4997
BanI G'GyrC_C
Cuts at: 2013 2427 2675 2710 2013
Size: 414 248 35 4300
Fragments arranged by size:
4300 414 248 35
BanII G_rGCy'C
Cuts at: 789 1549 1818 2011 3041 789
Size: 760 269 193 1030 2745
Fragments arranged by size:
2745 1030 760 269 193
BbeI G_GCGC'C
Cuts at: 2431 2679 2431
Size: 248 4749
BbsI GAAGACnn'nnnn_
Cuts at: 492 1056 1649 492
Size: 564 593 3840
Fragments arranged by size:
3840 593 564
BciVI GTATCCnnnnn_n'
Cuts at: 2895 3656 4997 2895
Size: 761 1341 2895
Fragments arranged by size:
2895 1341 761
BclI T'GATC_A
Cuts at: 2517 2517
Size: 4997
BglII A'GATC_T
Cuts at: 2512 4549 2512
Size: 2037 2960
BlpI GC'TnA_GC
Cuts at: 134 159 2109 134
Size: 25 1950 3022
Fragments arranged by size:
3022 1950 25
Bme1580I G_kGCm'C
Cuts at: 1549 1818 2622 2715 3771 1549
Size: 269 804 93 1056 2775
Fragments arranged by size:
2775 1056 804 269 93
BmrI ACTGGGnnnn_n'
Cuts at: 368 508 2209 2290 2625 4769 368
Size: 140 1701 81 335 2144 596
Fragments arranged by size:
2144 1701 596 335 140 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3364 3364
Size: 4997
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 884 931 1169 1329 439
Size: 445 47 238 160 4107
Fragments arranged by size:
4107 445 238 160 47
Bpu10I CC'TnA_GC
Cuts at: 956 956
Size: 4997
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2173 3052 3544 3842 2173
Size: 879 492 298 3328
Fragments arranged by size:
3328 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 556 4701 556
Size: 4145 852
BsaAI yAC'GTr
Cuts at: 2350 2350
Size: 4997
BsaBI GATnn'nnATC
Cuts at: 6 263 2511 4548 6
Size: 257 2248 2037 455
Fragments arranged by size:
2248 2037 455 257
BsaHI Gr'CG_yC
Cuts at: 1409 2428 2676 3378 4874 1409
Size: 1019 248 702 1496 1532
Fragments arranged by size:
1532 1496 1019 702 248
BsaWI w'CCGG_w
Cuts at: 315 761 1384 2186 2707 3385 3659 3806 4217
Size: 446 623 802 521 678 274 147 411
Cuts at: 4217 315
Size: 1095
Fragments arranged by size:
1095 802 678 623 521 446 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 555 585 1339 1369 555
Size: 30 754 30 4183
Fragments arranged by size:
4183 754 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 211 342 563 1978 211
Size: 131 221 1415 3230
Fragments arranged by size:
3230 1415 221 131
BseYI C'CCAG_C
Cuts at: 2422 3180 3757 4253 2422
Size: 758 577 496 3166
Fragments arranged by size:
3166 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1199 1210 1199
Size: 11 4986
BsiEI CG_ry'CG
Cuts at: 104 828 2539 2585 3793 104
Size: 724 1711 46 1208 1308
Fragments arranged by size:
1711 1308 1208 724 46
BsiHKAI G_wGCw'C
Cuts at: 370 455 2011 2789 2979 3771 370
Size: 85 1556 778 190 792 1596
Fragments arranged by size:
1596 1556 792 778 190 85
BsmI GAATG_Cn'
Cuts at: 1266 1266
Size: 4997
BsmBI CGTCTCn'nnnn_
Cuts at: 1400 1400
Size: 4997
Bsp1286I G_dGCh'C
Cuts at: 370 455 789 1549 1818 2011 2622 2715 2789
Size: 85 334 760 269 193 611 93 74
Cuts at: 2789 2979 3041 3771 370
Size: 190 62 730 1596
Fragments arranged by size:
1596 760 730 611 334 269 193 190
93 85 74 62
BspEI T'CCGG_A
Cuts at: 4217 4217
Size: 4997
BspHI T'CATG_A
Cuts at: 4173 4173
Size: 4997
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1468 2047 2261 2563 2944 1468
Size: 579 214 302 381 3521
Fragments arranged by size:
3521 579 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1629 3289 3343 4827 4992 50
Size: 1579 1660 54 1484 165 55
Fragments arranged by size:
1660 1579 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2909 2909
Size: 4997
BsrFI r'CCGG_y
Cuts at: 2995 3176 3385 2995
Size: 181 209 4607
Fragments arranged by size:
4607 209 181
BsrGI T'GTAC_A
Cuts at: 1355 1355
Size: 4997
BssSI C'ACGA_G
Cuts at: 3268 3626 3268
Size: 358 4639
BstAPI GCAn_nnn'nTGC
Cuts at: 2374 2374
Size: 4997
BstBI TT'CG_AA
Cuts at: 3358 3358
Size: 4997
BstEII G'GTnAC_C
Cuts at: 513 1501 513
Size: 988 4009
BstYI r'GATC_y
Cuts at: 1 725 2022 2489 2512 2847 3093 4105 4191
Size: 724 1297 467 23 335 246 1012 86
Cuts at: 4191 4203 4549 1
Size: 12 346 449
Fragments arranged by size:
1297 1012 724 467 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2947 3128 3291 2947
Size: 181 163 4653
Fragments arranged by size:
4653 181 163
BtsI GCAGTG_nn'
Cuts at: 436 1437 1547 2240 436
Size: 1001 110 693 3193
Fragments arranged by size:
3193 1001 693 110
DraI TTT'AAA
Cuts at: 1996 1996
Size: 4997
DrdI GACnn_nn'nnGTC
Cuts at: 1594 2703 3561 1594
Size: 1109 858 3030
Fragments arranged by size:
3030 1109 858
EaeI y'GGCC_r
Cuts at: 636 825 1775 2582 2756 3147 3174 4519 636
Size: 189 950 807 174 391 27 1345 1114
Fragments arranged by size:
1345 1114 950 807 391 189 174 27
EagI C'GGCC_G
Cuts at: 825 2582 825
Size: 1757 3240
EarI CTCTTCn'nnn_
Cuts at: 170 188 590 3020 3230 170
Size: 18 402 2430 210 1937
Fragments arranged by size:
2430 1937 402 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1782 3205 3515 3661 4807 1782
Size: 1423 310 146 1146 1972
Fragments arranged by size:
1972 1423 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2009 2009
Size: 4997
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 614 815 884 931 1169 1329 2822 3254
Size: 175 201 69 47 238 160 1493 432
Cuts at: 3254 4001 4269 439
Size: 747 268 1167
Fragments arranged by size:
1493 1167 747 432 268 238 201 175
160 69 47
EcoO109I rG'GnC_Cy
Cuts at: 422 1253 1925 2136 422
Size: 831 672 211 3283
Fragments arranged by size:
3283 831 672 211
EcoRI G'AATT_C
Cuts at: 2001 2001
Size: 4997
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 174 3016 174
Size: 2842 2155
FspI TGC'GCA
Cuts at: 2778 2778
Size: 4997
HaeII r_GCGC'y
Cuts at: 159 1725 2431 2679 3701 4680 159
Size: 1566 706 248 1022 979 476
Fragments arranged by size:
1566 1022 979 706 476 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 555 587 1309 1341 1774 1806 2839 2869 2871
Size: 32 722 32 433 32 1033 30 2
Cuts at: 2871 2901 555
Size: 30 2651
Fragments arranged by size:
2651 1033 722 433 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 704 2036 704
Size: 1332 3665
KasI G'GCGC_C
Cuts at: 2427 2675 2427
Size: 248 4749
KpnI G_GTAC'C
Cuts at: 2017 2017
Size: 4997
MluI A'CGCG_T
Cuts at: 4430 4430
Size: 4997
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 827 1786 1857 3668 3852 827
Size: 959 71 1811 184 1972
Fragments arranged by size:
1972 1811 959 184 71
MscI TGG'CCA
Cuts at: 638 1777 2758 638
Size: 1139 981 2877
Fragments arranged by size:
2877 1139 981
MslI CAynn'nnrTG
Cuts at: 520 931 1540 1901 2986 3113 520
Size: 411 609 361 1085 127 2404
Fragments arranged by size:
2404 1085 609 411 361 127
MspA1I CmG'CkG
Cuts at: 459 824 1194 1210 2108 2422 2782 3795 4040
Size: 365 370 16 898 314 360 1013 245
Cuts at: 4040 459
Size: 1416
Fragments arranged by size:
1416 1013 898 370 365 360 314 245
16
NaeI GCC'GGC
Cuts at: 3178 3178
Size: 4997
NarI GG'CG_CC
Cuts at: 2428 2676 2428
Size: 248 4749
NgoMIV G'CCGG_C
Cuts at: 3176 3176
Size: 4997
NspI r_CATG'y
Cuts at: 1323 2050 2550 3457 1323
Size: 727 500 907 2863
Fragments arranged by size:
2863 907 727 500
PciI A'CATG_T
Cuts at: 3453 3453
Size: 4997
PfoI T'CCnGG_A
Cuts at: 1306 1306
Size: 4997
PmeI GTTT'AAAC
Cuts at: 1996 1996
Size: 4997
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4136 4168 4136
Size: 32 4965
PpuMI rG'GwC_Cy
Cuts at: 422 1253 1925 422
Size: 831 672 3494
Fragments arranged by size:
3494 831 672
PspOMI G'GGCC_C
Cuts at: 1545 1814 1545
Size: 269 4728
PstI C_TGCA'G
Cuts at: 431 464 2044 2729 431
Size: 33 1580 685 2699
Fragments arranged by size:
2699 1580 685 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4997
PvuII CAG'CTG
Cuts at: 459 1210 2422 2782 459
Size: 751 1212 360 2674
Fragments arranged by size:
2674 1212 751 360
RsrII CG'GwC_CG
Cuts at: 3192 3192
Size: 4997
SacI G_AGCT'C
Cuts at: 2011 2011
Size: 4997
SalI G'TCGA_C
Cuts at: 2034 2034
Size: 4997
SanDI GG'GwC_CC
Cuts at: 1253 1253
Size: 4997
SapI GCTCTTCn'nnn_
Cuts at: 3020 3230 3020
Size: 210 4787
SbfI CC_TGCA'GG
Cuts at: 2044 2044
Size: 4997
ScaI AGT'ACT
Cuts at: 4514 4514
Size: 4997
SfcI C'TryA_G
Cuts at: 33 427 460 819 2040 2725 3718 3909 33
Size: 394 33 359 1221 685 993 191 1121
Fragments arranged by size:
1221 1121 993 685 394 359 191 33
SfoI GGC'GCC
Cuts at: 2429 2677 2429
Size: 248 4749
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4997
SmaI CCC'GGG
Cuts at: 2019 2019
Size: 4997
SmlI C'TyrA_G
Cuts at: 2152 3067 3559 3821 2152
Size: 915 492 262 3328
Fragments arranged by size:
3328 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4997
SphI G_CATG'C
Cuts at: 2050 2550 2050
Size: 500 4497
StyI C'CwwG_G
Cuts at: 535 1019 1506 2131 2485 535
Size: 484 487 625 354 3047
Fragments arranged by size:
3047 625 487 484 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 532 1080 2580 2713 3172 3381 532
Size: 548 1500 133 459 209 2148
Fragments arranged by size:
2148 1500 548 459 209 133
TatI w'GTAC_w
Cuts at: 1232 1355 4512 1232
Size: 123 3157 1717
Fragments arranged by size:
3157 1717 123
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1060 1092 1743 1775 4514 4546 1060
Size: 32 651 32 2739 32 1511
Fragments arranged by size:
2739 1511 651 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2794 2794
Size: 4997
XbaI T'CTAG_A
Cuts at: 2028 2028
Size: 4997
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 918 1006 1929 2492 918
Size: 88 923 563 3423
Fragments arranged by size:
3423 923 563 88
XmaI C'CCGG_G
Cuts at: 2017 2017
Size: 4997
XmnI GAAnn'nnTTC
Cuts at: 242 242
Size: 4997
ZraI GAC'GTC
Cuts at: 1410 1410
Size: 4997
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AlfI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmgBI BmtI BsiWI BssHII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI