Restriction map for
pF1KB8370
(Circular) (Six-base) MAPSORT of: pF1KB8370.seq Check: 306 from: 1 to: 3596
pF1KB8370 3596 bp
With 182 enzymes: SgfI *
July 2, 2008 13:23 ..
AccI GT'mk_AC
Cuts at: 634 634
Size: 3596
Acc65I G'GTAC_C
Cuts at: 612 612
Size: 3596
AclI AA'CG_TT
Cuts at: 3436 3436
Size: 3596
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1421 1853 2600 2868 1421
Size: 432 747 268 2149
Fragments arranged by size:
2149 747 432 268
AflIII A'CryG_T
Cuts at: 948 2052 3029 948
Size: 1104 977 1515
Fragments arranged by size:
1515 1104 977
AgeI A'CCGG_T
Cuts at: 1984 1984
Size: 3596
AlwNI CAG_nnn'CTG
Cuts at: 577 901 2468 577
Size: 324 1567 1705
Fragments arranged by size:
1705 1567 324
ApaLI G'TGCA_C
Cuts at: 2366 2366
Size: 3596
ApoI r'AATT_y
Cuts at: 169 600 2949 3205 169
Size: 431 2349 256 560
Fragments arranged by size:
2349 560 431 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3596
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3596
AvaI C'yCGr_G
Cuts at: 122 232 616 122
Size: 110 384 3102
Fragments arranged by size:
3102 384 110
BamHI G'GATC_C
Cuts at: 621 621
Size: 3596
BanI G'GyrC_C
Cuts at: 420 612 1026 1274 1309 420
Size: 192 414 248 35 2707
Fragments arranged by size:
2707 414 248 192 35
BanII G_rGCy'C
Cuts at: 610 1640 610
Size: 1030 2566
BbeI G_GCGC'C
Cuts at: 1030 1278 1030
Size: 248 3348
BciVI GTATCCnnnnn_n'
Cuts at: 1494 2255 3596 1494
Size: 761 1341 1494
Fragments arranged by size:
1494 1341 761
BclI T'GATC_A
Cuts at: 1116 1116
Size: 3596
BglII A'GATC_T
Cuts at: 1111 3148 1111
Size: 2037 1559
BlpI GC'TnA_GC
Cuts at: 409 708 409
Size: 299 3297
Bme1580I G_kGCm'C
Cuts at: 1221 1314 2370 1221
Size: 93 1056 2447
Fragments arranged by size:
2447 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 369 808 889 1224 3368 369
Size: 439 81 335 2144 597
Fragments arranged by size:
2144 597 439 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1963 1963
Size: 3596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 313 599 154
Size: 159 286 3151
Fragments arranged by size:
3151 286 159
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 1651 2143 2441 772
Size: 879 492 298 1927
Fragments arranged by size:
1927 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 237 3300 237
Size: 3063 533
BsaAI yAC'GTr
Cuts at: 949 949
Size: 3596
BsaBI GATnn'nnATC
Cuts at: 6 1110 3147 6
Size: 1104 2037 455
Fragments arranged by size:
2037 1104 455
BsaHI Gr'CG_yC
Cuts at: 1027 1275 1977 3473 1027
Size: 248 702 1496 1150
Fragments arranged by size:
1496 1150 702 248
BsaWI w'CCGG_w
Cuts at: 785 1306 1984 2258 2405 2816 785
Size: 521 678 274 147 411 1565
Fragments arranged by size:
1565 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 418 444 447 450 453 456 465 562 418
Size: 26 3 3 3 3 9 97 3452
Fragments arranged by size:
3452 97 26 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 1021 1779 2356 2852 1021
Size: 758 577 496 1765
Fragments arranged by size:
1765 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1138 1184 2392 104
Size: 1034 46 1208 1308
Fragments arranged by size:
1308 1208 1034 46
BsiHKAI G_wGCw'C
Cuts at: 610 1388 1578 2370 610
Size: 778 190 792 1836
Fragments arranged by size:
1836 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 610 1221 1314 1388 1578 1640 2370 610
Size: 611 93 74 190 62 730 1836
Fragments arranged by size:
1836 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2816 2816
Size: 3596
BspHI T'CATG_A
Cuts at: 2772 2772
Size: 3596
BspMI ACCTGCnnnn'nnnn_
Cuts at: 494 646 860 1162 1543 494
Size: 152 214 302 381 2547
Fragments arranged by size:
2547 381 302 214 152
BsrBI CCG'CTC
Cuts at: 50 162 1888 1942 3426 3591 50
Size: 112 1726 54 1484 165 55
Fragments arranged by size:
1726 1484 165 112 55 54
BsrDI GCAATG_nn'
Cuts at: 1508 1508
Size: 3596
BsrFI r'CCGG_y
Cuts at: 417 1594 1775 1984 417
Size: 1177 181 209 2029
Fragments arranged by size:
2029 1177 209 181
BssSI C'ACGA_G
Cuts at: 1867 2225 1867
Size: 358 3238
BstAPI GCAn_nnn'nTGC
Cuts at: 973 973
Size: 3596
BstBI TT'CG_AA
Cuts at: 1957 1957
Size: 3596
BstXI CCAn_nnnn'nTGG
Cuts at: 382 382
Size: 3596
BstYI r'GATC_y
Cuts at: 1 357 621 1088 1111 1446 1692 2704 2790
Size: 356 264 467 23 335 246 1012 86
Cuts at: 2790 2802 3148 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 356 346 335 264 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 269 107
Size: 162 3434
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1546 1727 1890 1546
Size: 181 163 3252
Fragments arranged by size:
3252 181 163
BtsI GCAGTG_nn'
Cuts at: 839 839
Size: 3596
DraI TTT'AAA
Cuts at: 595 595
Size: 3596
DrdI GACnn_nn'nnGTC
Cuts at: 1302 2160 1302
Size: 858 2738
EaeI y'GGCC_r
Cuts at: 1181 1355 1746 1773 3118 1181
Size: 174 391 27 1345 1659
Fragments arranged by size:
1659 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1181 1181
Size: 3596
EarI CTCTTCn'nnn_
Cuts at: 539 1619 1829 539
Size: 1080 210 2306
Fragments arranged by size:
2306 1080 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 139 1804 2114 2260 3406 139
Size: 1665 310 146 1146 329
Fragments arranged by size:
1665 1146 329 310 146
EcoICRI GAG'CTC
Cuts at: 608 608
Size: 3596
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 313 599 1421 1853 2600 2868 154
Size: 159 286 822 432 747 268 882
Fragments arranged by size:
882 822 747 432 286 268 159
EcoO109I rG'GnC_Cy
Cuts at: 321 449 735 321
Size: 128 286 3182
Fragments arranged by size:
3182 286 128
EcoRI G'AATT_C
Cuts at: 600 600
Size: 3596
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 322 1615 322
Size: 1293 2303
FspI TGC'GCA
Cuts at: 1377 1377
Size: 3596
HaeII r_GCGC'y
Cuts at: 141 1030 1278 2300 3279 141
Size: 889 248 1022 979 458
Fragments arranged by size:
1022 979 889 458 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1438 1468 1470 1500 1438
Size: 30 2 30 3534
Fragments arranged by size:
3534 30 30 2
HincII GTy'rAC
Cuts at: 635 635
Size: 3596
KasI G'GCGC_C
Cuts at: 1026 1274 1026
Size: 248 3348
KpnI G_GTAC'C
Cuts at: 616 616
Size: 3596
MluI A'CGCG_T
Cuts at: 3029 3029
Size: 3596
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 494 518 2267 2451 494
Size: 24 1749 184 1639
Fragments arranged by size:
1749 1639 184 24
MscI TGG'CCA
Cuts at: 1357 1357
Size: 3596
MslI CAynn'nnrTG
Cuts at: 274 1585 1712 274
Size: 1311 127 2158
Fragments arranged by size:
2158 1311 127
MspA1I CmG'CkG
Cuts at: 707 1021 1381 2394 2639 707
Size: 314 360 1013 245 1664
Fragments arranged by size:
1664 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1777 1777
Size: 3596
NarI GG'CG_CC
Cuts at: 1027 1275 1027
Size: 248 3348
NcoI C'CATG_G
Cuts at: 107 269 107
Size: 162 3434
NgoMIV G'CCGG_C
Cuts at: 1775 1775
Size: 3596
NspI r_CATG'y
Cuts at: 649 1149 2056 649
Size: 500 907 2189
Fragments arranged by size:
2189 907 500
PciI A'CATG_T
Cuts at: 2052 2052
Size: 3596
PmeI GTTT'AAAC
Cuts at: 595 595
Size: 3596
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2735 2767 2735
Size: 32 3564
PpuMI rG'GwC_Cy
Cuts at: 321 449 321
Size: 128 3468
PstI C_TGCA'G
Cuts at: 589 643 1328 589
Size: 54 685 2857
Fragments arranged by size:
2857 685 54
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3596
PvuII CAG'CTG
Cuts at: 1021 1381 1021
Size: 360 3236
RsrII CG'GwC_CG
Cuts at: 1791 1791
Size: 3596
SacI G_AGCT'C
Cuts at: 610 610
Size: 3596
SalI G'TCGA_C
Cuts at: 633 633
Size: 3596
SanDI GG'GwC_CC
Cuts at: 321 321
Size: 3596
SapI GCTCTTCn'nnn_
Cuts at: 1619 1829 1619
Size: 210 3386
SbfI CC_TGCA'GG
Cuts at: 643 643
Size: 3596
ScaI AGT'ACT
Cuts at: 3113 3113
Size: 3596
SfcI C'TryA_G
Cuts at: 33 585 639 1324 2317 2508 33
Size: 552 54 685 993 191 1121
Fragments arranged by size:
1121 993 685 552 191 54
SfoI GGC'GCC
Cuts at: 1028 1276 1028
Size: 248 3348
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3596
SmaI CCC'GGG
Cuts at: 618 618
Size: 3596
SmlI C'TyrA_G
Cuts at: 232 751 1666 2158 2420 232
Size: 519 915 492 262 1408
Fragments arranged by size:
1408 915 519 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3596
SphI G_CATG'C
Cuts at: 649 1149 649
Size: 500 3096
StyI C'CwwG_G
Cuts at: 107 269 315 527 730 1084 107
Size: 162 46 212 203 354 2619
Fragments arranged by size:
2619 354 212 203 162 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1179 1312 1771 1980 1179
Size: 133 459 209 2795
Fragments arranged by size:
2795 459 209 133
TatI w'GTAC_w
Cuts at: 3111 3111
Size: 3596
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3113 3145 3113
Size: 32 3564
Tth111I GACn'n_nGTC
Cuts at: 1393 1393
Size: 3596
XbaI T'CTAG_A
Cuts at: 627 627
Size: 3596
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1091 1091
Size: 3596
XhoI C'TCGA_G
Cuts at: 232 232
Size: 3596
XmaI C'CCGG_G
Cuts at: 616 616
Size: 3596
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XmnI ZraI