Restriction map for
pF1KB8368
(Circular) (Six-base) MAPSORT of: pF1KB8368.seq Check: 5538 from: 1 to: 4979
pF1KB8368 4979 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 172 250 1429 172
Size: 78 1179 3722
Fragments arranged by size:
3722 1179 78
AccI GT'mk_AC
Cuts at: 2017 2017
Size: 4979
Acc65I G'GTAC_C
Cuts at: 1995 1995
Size: 4979
AclI AA'CG_TT
Cuts at: 4819 4819
Size: 4979
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 995 2804 3236 3983 4251 995
Size: 1809 432 747 268 1723
Fragments arranged by size:
1809 1723 747 432 268
AflIII A'CryG_T
Cuts at: 2331 3435 4412 2331
Size: 1104 977 2898
Fragments arranged by size:
2898 1104 977
AgeI A'CCGG_T
Cuts at: 346 3367 346
Size: 3021 1958
AlwNI CAG_nnn'CTG
Cuts at: 922 2284 3851 922
Size: 1362 1567 2050
Fragments arranged by size:
2050 1567 1362
ApaI G_GGCC'C
Cuts at: 1435 1435
Size: 4979
ApaLI G'TGCA_C
Cuts at: 1442 3749 1442
Size: 2307 2672
ApoI r'AATT_y
Cuts at: 614 1983 4332 4588 614
Size: 1369 2349 256 1005
Fragments arranged by size:
2349 1369 1005 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4979
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4979
AvaI C'yCGr_G
Cuts at: 628 1717 1999 628
Size: 1089 282 3608
Fragments arranged by size:
3608 1089 282
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 738 771 800 833 738
Size: 33 29 33 4884
Fragments arranged by size:
4884 33 33 29
BamHI G'GATC_C
Cuts at: 1137 1336 1944 2004 1137
Size: 199 608 60 4112
Fragments arranged by size:
4112 608 199 60
BanI G'GyrC_C
Cuts at: 1788 1995 2409 2657 2692 1788
Size: 207 414 248 35 4075
Fragments arranged by size:
4075 414 248 207 35
BanII G_rGCy'C
Cuts at: 1435 1993 3023 1435
Size: 558 1030 3391
Fragments arranged by size:
3391 1030 558
BbeI G_GCGC'C
Cuts at: 2413 2661 2413
Size: 248 4731
BbsI GAAGACnn'nnnn_
Cuts at: 536 536
Size: 4979
BciVI GTATCCnnnnn_n'
Cuts at: 739 1566 2877 3638 4979 739
Size: 827 1311 761 1341 739
Fragments arranged by size:
1341 1311 827 761 739
BclI T'GATC_A
Cuts at: 2499 2499
Size: 4979
BfrBI ATG'CAT
Cuts at: 324 324
Size: 4979
BglI GCCn_nnn'nGGC
Cuts at: 371 1840 371
Size: 1469 3510
BglII A'GATC_T
Cuts at: 203 2494 4531 203
Size: 2291 2037 651
Fragments arranged by size:
2291 2037 651
BlpI GC'TnA_GC
Cuts at: 2091 2091
Size: 4979
Bme1580I G_kGCm'C
Cuts at: 1435 1446 2604 2697 3753 1435
Size: 11 1158 93 1056 2661
Fragments arranged by size:
2661 1158 1056 93 11
BmgBI CAC'GTC
Cuts at: 468 468
Size: 4979
BmrI ACTGGGnnnn_n'
Cuts at: 1284 2191 2272 2607 4751 1284
Size: 907 81 335 2144 1512
Fragments arranged by size:
2144 1512 907 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 334 3346 334
Size: 3012 1967
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 771 1903 771
Size: 1132 3847
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2155 3034 3526 3824 2155
Size: 879 492 298 3310
Fragments arranged by size:
3310 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 566 4683 566
Size: 4117 862
BsaAI yAC'GTr
Cuts at: 2332 2332
Size: 4979
BsaBI GATnn'nnATC
Cuts at: 6 950 2493 4530 6
Size: 944 1543 2037 455
Fragments arranged by size:
2037 1543 944 455
BsaHI Gr'CG_yC
Cuts at: 2410 2658 3360 4856 2410
Size: 248 702 1496 2533
Fragments arranged by size:
2533 1496 702 248
BsaWI w'CCGG_w
Cuts at: 346 2168 2689 3367 3641 3788 4199 346
Size: 1822 521 678 274 147 411 1126
Fragments arranged by size:
1822 1126 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 661 1369 1529 1923 2404 3162 3739 4235 661
Size: 708 160 394 481 758 577 496 1405
Fragments arranged by size:
1405 758 708 577 496 481 394 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1932 1932
Size: 4979
BsiEI CG_ry'CG
Cuts at: 104 2521 2567 3775 104
Size: 2417 46 1208 1308
Fragments arranged by size:
2417 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1446 1993 2771 2961 3753 1446
Size: 547 778 190 792 2672
Fragments arranged by size:
2672 792 778 547 190
Bsp1286I G_dGCh'C
Cuts at: 1435 1446 1993 2604 2697 2771 2961 3023 3753
Size: 11 547 611 93 74 190 62 730
Cuts at: 3753 1435
Size: 2661
Fragments arranged by size:
2661 730 611 547 190 93 74 62
11
BspEI T'CCGG_A
Cuts at: 4199 4199
Size: 4979
BspHI T'CATG_A
Cuts at: 407 4155 407
Size: 3748 1231
BspMI ACCTGCnnnn'nnnn_
Cuts at: 172 250 910 1429 1459 2029 2243 2545 2926
Size: 78 660 519 30 570 214 302 381
Cuts at: 2926 172
Size: 2225
Fragments arranged by size:
2225 660 570 519 381 302 214 78
30
BsrBI CCG'CTC
Cuts at: 50 3271 3325 4809 4974 50
Size: 3221 54 1484 165 55
Fragments arranged by size:
3221 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1672 2891 1672
Size: 1219 3760
BsrFI r'CCGG_y
Cuts at: 346 1446 2977 3158 3367 346
Size: 1100 1531 181 209 1958
Fragments arranged by size:
1958 1531 1100 209 181
BssSI C'ACGA_G
Cuts at: 3250 3608 3250
Size: 358 4621
BstAPI GCAn_nnn'nTGC
Cuts at: 2356 2356
Size: 4979
BstBI TT'CG_AA
Cuts at: 3340 3340
Size: 4979
BstEII G'GTnAC_C
Cuts at: 897 897
Size: 4979
BstXI CCAn_nnnn'nTGG
Cuts at: 661 1134 1927 661
Size: 473 793 3713
Fragments arranged by size:
3713 793 473
BstYI r'GATC_y
Cuts at: 1 203 650 821 1137 1336 1387 1794 1944
Size: 202 447 171 316 199 51 407 150
Cuts at: 1944 2004 2471 2494 2829 3075 4087 4173 4185
Size: 60 467 23 335 246 1012 86 12
Cuts at: 4185 4531 1
Size: 346 449
Fragments arranged by size:
1012 467 449 447 407 346 335 316
246 202 199 171 150 86 60 51
23 12
Bsu36I CC'TnA_GG
Cuts at: 1238 1238
Size: 4979
BtgI C'CryG_G
Cuts at: 741 741
Size: 4979
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2929 3110 3273 2929
Size: 181 163 4635
Fragments arranged by size:
4635 181 163
BtsI GCAGTG_nn'
Cuts at: 878 1892 2222 878
Size: 1014 330 3635
Fragments arranged by size:
3635 1014 330
DraI TTT'AAA
Cuts at: 210 1484 1978 210
Size: 1274 494 3211
Fragments arranged by size:
3211 1274 494
DrdI GACnn_nn'nnGTC
Cuts at: 2685 3543 2685
Size: 858 4121
EaeI y'GGCC_r
Cuts at: 1448 1883 2564 2738 3129 3156 4501 1448
Size: 435 681 174 391 27 1345 1926
Fragments arranged by size:
1926 1345 681 435 391 174 27
EagI C'GGCC_G
Cuts at: 2564 2564
Size: 4979
EarI CTCTTCn'nnn_
Cuts at: 1508 3002 3212 1508
Size: 1494 210 3275
Fragments arranged by size:
3275 1494 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 902 3187 3497 3643 4789 902
Size: 2285 310 146 1146 1092
Fragments arranged by size:
2285 1146 1092 310 146
EcoICRI GAG'CTC
Cuts at: 1991 1991
Size: 4979
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 771 995 1903 2804 3236 3983 4251 771
Size: 224 908 901 432 747 268 1499
Fragments arranged by size:
1499 908 901 747 432 268 224
EcoNI CCTnn'n_nnAGG
Cuts at: 718 718
Size: 4979
EcoO109I rG'GnC_Cy
Cuts at: 1284 1432 1842 2118 1284
Size: 148 410 276 4145
Fragments arranged by size:
4145 410 276 148
EcoRI G'AATT_C
Cuts at: 1983 1983
Size: 4979
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2998 2998
Size: 4979
FspI TGC'GCA
Cuts at: 2760 2760
Size: 4979
HaeII r_GCGC'y
Cuts at: 2413 2661 3683 4662 2413
Size: 248 1022 979 2730
Fragments arranged by size:
2730 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1135 1167 2821 2851 2853 2883 1135
Size: 32 1654 30 2 30 3231
Fragments arranged by size:
3231 1654 32 30 30 2
HincII GTy'rAC
Cuts at: 1653 2018 1653
Size: 365 4614
HindIII A'AGCT_T
Cuts at: 1548 1548
Size: 4979
KasI G'GCGC_C
Cuts at: 2409 2657 2409
Size: 248 4731
KpnI G_GTAC'C
Cuts at: 1999 1999
Size: 4979
MluI A'CGCG_T
Cuts at: 4412 4412
Size: 4979
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 938 3650 3834 938
Size: 2712 184 2083
Fragments arranged by size:
2712 2083 184
MscI TGG'CCA
Cuts at: 1885 2740 1885
Size: 855 4124
MslI CAynn'nnrTG
Cuts at: 388 409 1300 1331 1780 2968 3095 388
Size: 21 891 31 449 1188 127 2272
Fragments arranged by size:
2272 1188 891 449 127 31 21
MspA1I CmG'CkG
Cuts at: 640 1700 1923 2090 2404 2764 3777 4022 640
Size: 1060 223 167 314 360 1013 245 1597
Fragments arranged by size:
1597 1060 1013 360 314 245 223 167
NaeI GCC'GGC
Cuts at: 3160 3160
Size: 4979
NarI GG'CG_CC
Cuts at: 2410 2658 2410
Size: 248 4731
NcoI C'CATG_G
Cuts at: 741 741
Size: 4979
NgoMIV G'CCGG_C
Cuts at: 3158 3158
Size: 4979
NsiI A_TGCA'T
Cuts at: 326 326
Size: 4979
NspI r_CATG'y
Cuts at: 1804 1955 2032 2532 3439 1804
Size: 151 77 500 907 3344
Fragments arranged by size:
3344 907 500 151 77
PciI A'CATG_T
Cuts at: 3435 3435
Size: 4979
PflMI CCAn_nnn'nTGG
Cuts at: 742 922 742
Size: 180 4799
PfoI T'CCnGG_A
Cuts at: 189 699 189
Size: 510 4469
PmeI GTTT'AAAC
Cuts at: 1978 1978
Size: 4979
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4118 4150 4118
Size: 32 4947
PpuMI rG'GwC_Cy
Cuts at: 1284 1284
Size: 4979
PspOMI G'GGCC_C
Cuts at: 1431 1431
Size: 4979
PstI C_TGCA'G
Cuts at: 1440 2026 2711 1440
Size: 586 685 3708
Fragments arranged by size:
3708 685 586
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4979
PvuII CAG'CTG
Cuts at: 640 1923 2404 2764 640
Size: 1283 481 360 2855
Fragments arranged by size:
2855 1283 481 360
RsrII CG'GwC_CG
Cuts at: 3174 3174
Size: 4979
SacI G_AGCT'C
Cuts at: 1993 1993
Size: 4979
SalI G'TCGA_C
Cuts at: 2016 2016
Size: 4979
SanDI GG'GwC_CC
Cuts at: 1284 1284
Size: 4979
SapI GCTCTTCn'nnn_
Cuts at: 3002 3212 3002
Size: 210 4769
SbfI CC_TGCA'GG
Cuts at: 1440 2026 1440
Size: 586 4393
ScaI AGT'ACT
Cuts at: 4496 4496
Size: 4979
SfcI C'TryA_G
Cuts at: 33 888 1436 2022 2707 3700 3891 33
Size: 855 548 586 685 993 191 1121
Fragments arranged by size:
1121 993 855 685 586 548 191
SfoI GGC'GCC
Cuts at: 2411 2659 2411
Size: 248 4731
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4979
SmaI CCC'GGG
Cuts at: 1719 2001 1719
Size: 282 4697
SmlI C'TyrA_G
Cuts at: 2134 3049 3541 3803 2134
Size: 915 492 262 3310
Fragments arranged by size:
3310 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4979
SphI G_CATG'C
Cuts at: 2032 2532 2032
Size: 500 4479
SspI AAT'ATT
Cuts at: 1404 1404
Size: 4979
StuI AGG'CCT
Cuts at: 255 255
Size: 4979
StyI C'CwwG_G
Cuts at: 741 992 1625 1748 2113 2467 741
Size: 251 633 123 365 354 3253
Fragments arranged by size:
3253 633 365 354 251 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1121 1915 2562 2695 3154 3363 1121
Size: 794 647 133 459 209 2737
Fragments arranged by size:
2737 794 647 459 209 133
TatI w'GTAC_w
Cuts at: 380 4494 380
Size: 4114 865
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1276 1308 4496 4528 1276
Size: 32 3188 32 1727
Fragments arranged by size:
3188 1727 32 32
Tth111I GACn'n_nGTC
Cuts at: 2776 2776
Size: 4979
XbaI T'CTAG_A
Cuts at: 1507 2010 1507
Size: 503 4476
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 429 2474 429
Size: 2045 2934
XmaI C'CCGG_G
Cuts at: 1717 1999 1717
Size: 282 4697
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BbvCI BcgI BmtI Bpu10I BsaXI BseRI BsiWI
BsmI BsmBI BsrGI BssHII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI PacI PasI PmlI PshAI PsiI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
XmnI ZraI