Restriction map for
pF1KB8366
(Circular) (Six-base) MAPSORT of: pF1KB8366.seq Check: 8632 from: 1 to: 3647
pF1KB8366 3647 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 685 685
Size: 3647
Acc65I G'GTAC_C
Cuts at: 663 663
Size: 3647
AclI AA'CG_TT
Cuts at: 3487 3487
Size: 3647
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1472 1904 2651 2919 1472
Size: 432 747 268 2200
Fragments arranged by size:
2200 747 432 268
AflIII A'CryG_T
Cuts at: 999 2103 3080 999
Size: 1104 977 1566
Fragments arranged by size:
1566 1104 977
AgeI A'CCGG_T
Cuts at: 2035 2035
Size: 3647
AlwNI CAG_nnn'CTG
Cuts at: 465 952 2519 465
Size: 487 1567 1593
Fragments arranged by size:
1593 1567 487
ApaLI G'TGCA_C
Cuts at: 2417 2417
Size: 3647
ApoI r'AATT_y
Cuts at: 207 651 3000 3256 207
Size: 444 2349 256 598
Fragments arranged by size:
2349 598 444 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3647
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3647
AvaI C'yCGr_G
Cuts at: 667 667
Size: 3647
BamHI G'GATC_C
Cuts at: 672 672
Size: 3647
BanI G'GyrC_C
Cuts at: 133 311 663 1077 1325 1360 133
Size: 178 352 414 248 35 2420
Fragments arranged by size:
2420 414 352 248 178 35
BanII G_rGCy'C
Cuts at: 661 1691 661
Size: 1030 2617
BbeI G_GCGC'C
Cuts at: 137 1081 1329 137
Size: 944 248 2455
Fragments arranged by size:
2455 944 248
BciVI GTATCCnnnnn_n'
Cuts at: 1545 2306 3647 1545
Size: 761 1341 1545
Fragments arranged by size:
1545 1341 761
BclI T'GATC_A
Cuts at: 1167 1167
Size: 3647
BglII A'GATC_T
Cuts at: 1162 3199 1162
Size: 2037 1610
BlpI GC'TnA_GC
Cuts at: 759 759
Size: 3647
Bme1580I G_kGCm'C
Cuts at: 316 1272 1365 2421 316
Size: 956 93 1056 1542
Fragments arranged by size:
1542 1056 956 93
BmrI ACTGGGnnnn_n'
Cuts at: 859 940 1275 3419 859
Size: 81 335 2144 1087
Fragments arranged by size:
2144 1087 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2014 2014
Size: 3647
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 823 1702 2194 2492 823
Size: 879 492 298 1978
Fragments arranged by size:
1978 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 242 3351 242
Size: 3109 538
BsaAI yAC'GTr
Cuts at: 1000 1000
Size: 3647
BsaBI GATnn'nnATC
Cuts at: 6 1161 3198 6
Size: 1155 2037 455
Fragments arranged by size:
2037 1155 455
BsaHI Gr'CG_yC
Cuts at: 134 376 1078 1326 2028 3524 134
Size: 242 702 248 702 1496 257
Fragments arranged by size:
1496 702 702 257 248 242
BsaWI w'CCGG_w
Cuts at: 836 1357 2035 2309 2456 2867 836
Size: 521 678 274 147 411 1616
Fragments arranged by size:
1616 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 229 461 229
Size: 232 3415
BseYI C'CCAG_C
Cuts at: 1072 1830 2407 2903 1072
Size: 758 577 496 1816
Fragments arranged by size:
1816 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 147 287 507 147
Size: 140 220 3287
Fragments arranged by size:
3287 220 140
BsiEI CG_ry'CG
Cuts at: 104 1189 1235 2443 104
Size: 1085 46 1208 1308
Fragments arranged by size:
1308 1208 1085 46
BsiHKAI G_wGCw'C
Cuts at: 661 1439 1629 2421 661
Size: 778 190 792 1887
Fragments arranged by size:
1887 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 316 661 1272 1365 1439 1629 1691 2421 316
Size: 345 611 93 74 190 62 730 1542
Fragments arranged by size:
1542 730 611 345 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2867 2867
Size: 3647
BspHI T'CATG_A
Cuts at: 2823 2823
Size: 3647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 697 911 1213 1594 697
Size: 214 302 381 2750
Fragments arranged by size:
2750 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1939 1993 3477 3642 50
Size: 1889 54 1484 165 55
Fragments arranged by size:
1889 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1559 1559
Size: 3647
BsrFI r'CCGG_y
Cuts at: 1645 1826 2035 1645
Size: 181 209 3257
Fragments arranged by size:
3257 209 181
BssSI C'ACGA_G
Cuts at: 1918 2276 1918
Size: 358 3289
BstAPI GCAn_nnn'nTGC
Cuts at: 1024 1024
Size: 3647
BstBI TT'CG_AA
Cuts at: 2008 2008
Size: 3647
BstYI r'GATC_y
Cuts at: 1 672 1139 1162 1497 1743 2755 2841 2853
Size: 671 467 23 335 246 1012 86 12
Cuts at: 2853 3199 1
Size: 346 449
Fragments arranged by size:
1012 671 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 254 254
Size: 3647
BtgI C'CryG_G
Cuts at: 107 107
Size: 3647
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1597 1778 1941 1597
Size: 181 163 3303
Fragments arranged by size:
3303 181 163
BtsI GCAGTG_nn'
Cuts at: 148 890 148
Size: 742 2905
DraI TTT'AAA
Cuts at: 646 646
Size: 3647
DraIII CAC_nnn'GTG
Cuts at: 192 192
Size: 3647
DrdI GACnn_nn'nnGTC
Cuts at: 1353 2211 1353
Size: 858 2789
EaeI y'GGCC_r
Cuts at: 365 1232 1406 1797 1824 3169 365
Size: 867 174 391 27 1345 843
Fragments arranged by size:
1345 867 843 391 174 27
EagI C'GGCC_G
Cuts at: 1232 1232
Size: 3647
EarI CTCTTCn'nnn_
Cuts at: 545 620 1670 1880 545
Size: 75 1050 210 2312
Fragments arranged by size:
2312 1050 210 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1855 2165 2311 3457 1855
Size: 310 146 1146 2045
Fragments arranged by size:
2045 1146 310 146
EcoICRI GAG'CTC
Cuts at: 659 659
Size: 3647
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1472 1904 2651 2919 1472
Size: 432 747 268 2200
Fragments arranged by size:
2200 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 786 786
Size: 3647
EcoRI G'AATT_C
Cuts at: 207 651 207
Size: 444 3203
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1666 1666
Size: 3647
FspI TGC'GCA
Cuts at: 1428 1428
Size: 3647
HaeII r_GCGC'y
Cuts at: 137 1081 1329 2351 3330 137
Size: 944 248 1022 979 454
Fragments arranged by size:
1022 979 944 454 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1489 1519 1521 1551 1489
Size: 30 2 30 3585
Fragments arranged by size:
3585 30 30 2
HincII GTy'rAC
Cuts at: 429 686 429
Size: 257 3390
KasI G'GCGC_C
Cuts at: 133 1077 1325 133
Size: 944 248 2455
Fragments arranged by size:
2455 944 248
KpnI G_GTAC'C
Cuts at: 667 667
Size: 3647
MluI A'CGCG_T
Cuts at: 3080 3080
Size: 3647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2318 2502 2318
Size: 184 3463
MscI TGG'CCA
Cuts at: 1408 1408
Size: 3647
MslI CAynn'nnrTG
Cuts at: 1636 1763 1636
Size: 127 3520
MspA1I CmG'CkG
Cuts at: 131 758 1072 1432 2445 2690 131
Size: 627 314 360 1013 245 1088
Fragments arranged by size:
1088 1013 627 360 314 245
NaeI GCC'GGC
Cuts at: 1828 1828
Size: 3647
NarI GG'CG_CC
Cuts at: 134 1078 1326 134
Size: 944 248 2455
Fragments arranged by size:
2455 944 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3647
NgoMIV G'CCGG_C
Cuts at: 1826 1826
Size: 3647
NspI r_CATG'y
Cuts at: 530 700 1200 2107 530
Size: 170 500 907 2070
Fragments arranged by size:
2070 907 500 170
PasI CC'CwG_GG
Cuts at: 168 168
Size: 3647
PciI A'CATG_T
Cuts at: 2103 2103
Size: 3647
PmeI GTTT'AAAC
Cuts at: 646 646
Size: 3647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2786 2818 2786
Size: 32 3615
PstI C_TGCA'G
Cuts at: 694 1379 694
Size: 685 2962
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3647
PvuII CAG'CTG
Cuts at: 1072 1432 1072
Size: 360 3287
RsrII CG'GwC_CG
Cuts at: 1842 1842
Size: 3647
SacI G_AGCT'C
Cuts at: 661 661
Size: 3647
SalI G'TCGA_C
Cuts at: 684 684
Size: 3647
SapI GCTCTTCn'nnn_
Cuts at: 1670 1880 1670
Size: 210 3437
SbfI CC_TGCA'GG
Cuts at: 694 694
Size: 3647
ScaI AGT'ACT
Cuts at: 3164 3164
Size: 3647
SfcI C'TryA_G
Cuts at: 33 690 1375 2368 2559 33
Size: 657 685 993 191 1121
Fragments arranged by size:
1121 993 685 657 191
SfoI GGC'GCC
Cuts at: 135 1079 1327 135
Size: 944 248 2455
Fragments arranged by size:
2455 944 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3647
SmaI CCC'GGG
Cuts at: 669 669
Size: 3647
SmlI C'TyrA_G
Cuts at: 802 1717 2209 2471 802
Size: 915 492 262 1978
Fragments arranged by size:
1978 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3647
SphI G_CATG'C
Cuts at: 700 1200 700
Size: 500 3147
SspI AAT'ATT
Cuts at: 636 636
Size: 3647
StyI C'CwwG_G
Cuts at: 107 781 1135 107
Size: 674 354 2619
Fragments arranged by size:
2619 674 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1230 1363 1822 2031 1230
Size: 133 459 209 2846
Fragments arranged by size:
2846 459 209 133
TatI w'GTAC_w
Cuts at: 3162 3162
Size: 3647
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 204 236 3164 3196 204
Size: 32 2928 32 655
Fragments arranged by size:
2928 655 32 32
Tth111I GACn'n_nGTC
Cuts at: 1444 1444
Size: 3647
XbaI T'CTAG_A
Cuts at: 678 678
Size: 3647
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1142 1142
Size: 3647
XmaI C'CCGG_G
Cuts at: 667 667
Size: 3647
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI