Restriction map for
pF1KB8365
(Circular) (Six-base) MAPSORT of: pF1KB8365.seq Check: 9449 from: 1 to: 4529
pF1KB8365 4529 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1567 1567
Size: 4529
Acc65I G'GTAC_C
Cuts at: 1545 1545
Size: 4529
AclI AA'CG_TT
Cuts at: 4369 4369
Size: 4529
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2354 2786 3533 3801 2354
Size: 432 747 268 3082
Fragments arranged by size:
3082 747 432 268
AfeI AGC'GCT
Cuts at: 745 901 745
Size: 156 4373
AflIII A'CryG_T
Cuts at: 302 863 1881 2985 3962 302
Size: 561 1018 1104 977 869
Fragments arranged by size:
1104 1018 977 869 561
AgeI A'CCGG_T
Cuts at: 2917 2917
Size: 4529
AlwNI CAG_nnn'CTG
Cuts at: 149 1110 1377 1834 3401 149
Size: 961 267 457 1567 1277
Fragments arranged by size:
1567 1277 961 457 267
ApaI G_GGCC'C
Cuts at: 493 1091 1199 493
Size: 598 108 3823
Fragments arranged by size:
3823 598 108
ApaLI G'TGCA_C
Cuts at: 1045 3299 1045
Size: 2254 2275
ApoI r'AATT_y
Cuts at: 1533 3882 4138 1533
Size: 2349 256 1924
Fragments arranged by size:
2349 1924 256
AscI GG'CGCG_CC
Cuts at: 1073 1073
Size: 4529
AseI AT'TA_AT
Cuts at: 20 20
Size: 4529
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4529
AvaI C'yCGr_G
Cuts at: 318 435 1084 1198 1549 318
Size: 117 649 114 351 3298
Fragments arranged by size:
3298 649 351 117 114
AvrII C'CTAG_G
Cuts at: 324 324
Size: 4529
BamHI G'GATC_C
Cuts at: 1554 1554
Size: 4529
BanI G'GyrC_C
Cuts at: 379 448 663 964 1003 1141 1245 1545 1959
Size: 69 215 301 39 138 104 300 414
Cuts at: 1959 2207 2242 379
Size: 248 35 2666
Fragments arranged by size:
2666 414 301 300 248 215 138 104
69 39 35
BanII G_rGCy'C
Cuts at: 226 373 436 493 526 832 1091 1199 1453
Size: 147 63 57 33 306 259 108 254
Cuts at: 1453 1463 1543 2573 226
Size: 10 80 1030 2182
Fragments arranged by size:
2182 1030 306 259 254 147 108 80
63 57 33 10
BbeI G_GCGC'C
Cuts at: 383 452 667 968 1007 1145 1963 2211 383
Size: 69 215 301 39 138 818 248 2701
Fragments arranged by size:
2701 818 301 248 215 138 69 39
BbsI GAAGACnn'nnnn_
Cuts at: 1060 1162 1060
Size: 102 4427
BbvCI CC'TCA_GC
Cuts at: 1464 1464
Size: 4529
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 849 883 849
Size: 34 4495
BciVI GTATCCnnnnn_n'
Cuts at: 2427 3188 4529 2427
Size: 761 1341 2427
Fragments arranged by size:
2427 1341 761
BclI T'GATC_A
Cuts at: 2049 2049
Size: 4529
BglI GCCn_nnn'nGGC
Cuts at: 440 566 440
Size: 126 4403
BglII A'GATC_T
Cuts at: 692 2044 4081 692
Size: 1352 2037 1140
Fragments arranged by size:
2037 1352 1140
BlpI GC'TnA_GC
Cuts at: 430 685 1641 430
Size: 255 956 3318
Fragments arranged by size:
3318 956 255
Bme1580I G_kGCm'C
Cuts at: 493 1049 1091 1199 1220 2154 2247 3303 493
Size: 556 42 108 21 934 93 1056 1719
Fragments arranged by size:
1719 1056 934 556 108 93 42 21
BmrI ACTGGGnnnn_n'
Cuts at: 1741 1822 2157 4301 1741
Size: 81 335 2144 1969
Fragments arranged by size:
2144 1969 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 201 2896 201
Size: 2695 1834
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1145 1492 1145
Size: 347 4182
Bpu10I CC'TnA_GC
Cuts at: 1464 1464
Size: 4529
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1705 2584 3076 3374 1705
Size: 879 492 298 2860
Fragments arranged by size:
2860 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4233 4233
Size: 4529
BsaAI yAC'GTr
Cuts at: 1882 1882
Size: 4529
BsaBI GATnn'nnATC
Cuts at: 6 2043 4080 6
Size: 2037 2037 455
Fragments arranged by size:
2037 2037 455
BsaHI Gr'CG_yC
Cuts at: 292 380 449 581 664 965 1004 1142 1960
Size: 88 69 132 83 301 39 138 818
Cuts at: 1960 2208 2910 4406 292
Size: 248 702 1496 415
Fragments arranged by size:
1496 818 702 415 301 248 138 132
88 83 69 39
BsaWI w'CCGG_w
Cuts at: 1718 2239 2917 3191 3338 3749 1718
Size: 521 678 274 147 411 2498
Fragments arranged by size:
2498 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 838 868 1291 1321 1465 1495 838
Size: 30 423 30 144 30 3872
Fragments arranged by size:
3872 423 144 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 177 1158 1320 177
Size: 981 162 3386
Fragments arranged by size:
3386 981 162
BseYI C'CCAG_C
Cuts at: 667 1015 1259 1954 2712 3289 3785 667
Size: 348 244 695 758 577 496 1411
Fragments arranged by size:
1411 758 695 577 496 348 244
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 1249 829
Size: 420 4109
BsiEI CG_ry'CG
Cuts at: 104 404 503 2071 2117 3325 104
Size: 300 99 1568 46 1208 1308
Fragments arranged by size:
1568 1308 1208 300 99 46
BsiHKAI G_wGCw'C
Cuts at: 1049 1543 2321 2511 3303 1049
Size: 494 778 190 792 2275
Fragments arranged by size:
2275 792 778 494 190
BsiWI C'GTAC_G
Cuts at: 533 533
Size: 4529
Bsp1286I G_dGCh'C
Cuts at: 226 373 436 493 526 832 1049 1091 1199
Size: 147 63 57 33 306 217 42 108
Cuts at: 1199 1220 1453 1463 1543 2154 2247 2321 2511
Size: 21 233 10 80 611 93 74 190
Cuts at: 2511 2573 3303 226
Size: 62 730 1452
Fragments arranged by size:
1452 730 611 306 233 217 190 147
108 93 80 74 63 62 57 42
33 21 10
BspEI T'CCGG_A
Cuts at: 3749 3749
Size: 4529
BspHI T'CATG_A
Cuts at: 3705 3705
Size: 4529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1579 1793 2095 2476 1579
Size: 214 302 381 3632
Fragments arranged by size:
3632 381 302 214
BsrBI CCG'CTC
Cuts at: 50 275 440 947 2821 2875 4359 4524 50
Size: 225 165 507 1874 54 1484 165 55
Fragments arranged by size:
1874 1484 507 225 165 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2441 2441
Size: 4529
BsrFI r'CCGG_y
Cuts at: 331 355 403 728 928 1057 1077 1476 2527
Size: 24 48 325 200 129 20 399 1051
Cuts at: 2527 2708 2917 331
Size: 181 209 1943
Fragments arranged by size:
1943 1051 399 325 209 200 181 129
48 24 20
BssHII G'CGCG_C
Cuts at: 1073 1073
Size: 4529
BssSI C'ACGA_G
Cuts at: 1252 2800 3158 1252
Size: 1548 358 2623
Fragments arranged by size:
2623 1548 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1906 1906
Size: 4529
BstBI TT'CG_AA
Cuts at: 2890 2890
Size: 4529
BstEII G'GTnAC_C
Cuts at: 1437 1437
Size: 4529
BstYI r'GATC_y
Cuts at: 1 692 1554 2021 2044 2379 2625 3637 3723
Size: 691 862 467 23 335 246 1012 86
Cuts at: 3723 3735 4081 1
Size: 12 346 449
Fragments arranged by size:
1012 862 691 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 416 452 527 647 416
Size: 36 75 120 4298
Fragments arranged by size:
4298 120 75 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2479 2660 2823 2479
Size: 181 163 4185
Fragments arranged by size:
4185 181 163
BtsI GCAGTG_nn'
Cuts at: 1375 1772 1375
Size: 397 4132
DraI TTT'AAA
Cuts at: 1528 1528
Size: 4529
DrdI GACnn_nn'nnGTC
Cuts at: 291 790 2235 3093 291
Size: 499 1445 858 1727
Fragments arranged by size:
1727 1445 858 499
EaeI y'GGCC_r
Cuts at: 401 413 500 650 1059 2114 2288 2679 2706
Size: 12 87 150 409 1055 174 391 27
Cuts at: 2706 4051 401
Size: 1345 879
Fragments arranged by size:
1345 1055 879 409 391 174 150 87
27 12
EagI C'GGCC_G
Cuts at: 401 500 2114 401
Size: 99 1614 2816
Fragments arranged by size:
2816 1614 99
EarI CTCTTCn'nnn_
Cuts at: 721 2552 2762 721
Size: 1831 210 2488
Fragments arranged by size:
2488 1831 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 751 2737 3047 3193 4339 751
Size: 1986 310 146 1146 941
Fragments arranged by size:
1986 1146 941 310 146
EcoICRI GAG'CTC
Cuts at: 1541 1541
Size: 4529
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1145 1492 2354 2786 3533 3801 1145
Size: 347 862 432 747 268 1873
Fragments arranged by size:
1873 862 747 432 347 268
EcoO109I rG'GnC_Cy
Cuts at: 490 993 1278 1668 490
Size: 503 285 390 3351
Fragments arranged by size:
3351 503 390 285
EcoRI G'AATT_C
Cuts at: 1533 1533
Size: 4529
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1371 2548 1371
Size: 1177 3352
FseI GG_CCGG'CC
Cuts at: 335 335
Size: 4529
FspI TGC'GCA
Cuts at: 461 2310 461
Size: 1849 2680
HaeII r_GCGC'y
Cuts at: 383 430 452 667 747 903 968 1007 1022
Size: 47 22 215 80 156 65 39 15
Cuts at: 1022 1145 1207 1433 1963 2211 3233 4212 383
Size: 123 62 226 530 248 1022 979 700
Fragments arranged by size:
1022 979 700 530 248 226 215 156
123 80 65 62 47 39 22 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2371 2401 2403 2433 2371
Size: 30 2 30 4467
Fragments arranged by size:
4467 30 30 2
HincII GTy'rAC
Cuts at: 1568 1568
Size: 4529
KasI G'GCGC_C
Cuts at: 379 448 663 964 1003 1141 1959 2207 379
Size: 69 215 301 39 138 818 248 2701
Fragments arranged by size:
2701 818 301 248 215 138 69 39
KpnI G_GTAC'C
Cuts at: 1549 1549
Size: 4529
MluI A'CGCG_T
Cuts at: 3962 3962
Size: 4529
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 569 3200 3384 569
Size: 2631 184 1714
Fragments arranged by size:
2631 1714 184
MscI TGG'CCA
Cuts at: 652 2290 652
Size: 1638 2891
MslI CAynn'nnrTG
Cuts at: 247 253 646 1040 2518 2645 247
Size: 6 393 394 1478 127 2131
Fragments arranged by size:
2131 1478 394 393 127 6
MspA1I CmG'CkG
Cuts at: 962 1263 1640 1954 2314 3327 3572 962
Size: 301 377 314 360 1013 245 1919
Fragments arranged by size:
1919 1013 377 360 314 301 245
NaeI GCC'GGC
Cuts at: 333 357 405 930 1079 1478 2710 333
Size: 24 48 525 149 399 1232 2152
Fragments arranged by size:
2152 1232 525 399 149 48 24
NarI GG'CG_CC
Cuts at: 380 449 664 965 1004 1142 1960 2208 380
Size: 69 215 301 39 138 818 248 2701
Fragments arranged by size:
2701 818 301 248 215 138 69 39
NcoI C'CATG_G
Cuts at: 416 452 527 647 416
Size: 36 75 120 4298
Fragments arranged by size:
4298 120 75 36
NgoMIV G'CCGG_C
Cuts at: 331 355 403 928 1077 1476 2708 331
Size: 24 48 525 149 399 1232 2152
Fragments arranged by size:
2152 1232 525 399 149 48 24
NotI GC'GGCC_GC
Cuts at: 500 500
Size: 4529
NspI r_CATG'y
Cuts at: 306 363 867 1582 2082 2989 306
Size: 57 504 715 500 907 1846
Fragments arranged by size:
1846 907 715 504 500 57
PciI A'CATG_T
Cuts at: 302 863 2985 302
Size: 561 2122 1846
Fragments arranged by size:
2122 1846 561
PflMI CCAn_nnn'nTGG
Cuts at: 149 1110 149
Size: 961 3568
PmeI GTTT'AAAC
Cuts at: 1528 1528
Size: 4529
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3668 3700 3668
Size: 32 4497
PpuMI rG'GwC_Cy
Cuts at: 993 1278 993
Size: 285 4244
PspOMI G'GGCC_C
Cuts at: 489 1087 1195 489
Size: 598 108 3823
Fragments arranged by size:
3823 598 108
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 145 177 865 897 145
Size: 32 688 32 3777
Fragments arranged by size:
3777 688 32 32
PstI C_TGCA'G
Cuts at: 1576 2261 1576
Size: 685 3844
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4529
PvuII CAG'CTG
Cuts at: 1263 1954 2314 1263
Size: 691 360 3478
Fragments arranged by size:
3478 691 360
RsrII CG'GwC_CG
Cuts at: 2724 2724
Size: 4529
SacI G_AGCT'C
Cuts at: 1543 1543
Size: 4529
SalI G'TCGA_C
Cuts at: 1566 1566
Size: 4529
SanDI GG'GwC_CC
Cuts at: 1278 1278
Size: 4529
SapI GCTCTTCn'nnn_
Cuts at: 2552 2762 2552
Size: 210 4319
SbfI CC_TGCA'GG
Cuts at: 1576 1576
Size: 4529
ScaI AGT'ACT
Cuts at: 4046 4046
Size: 4529
SfcI C'TryA_G
Cuts at: 33 1572 2257 3250 3441 33
Size: 1539 685 993 191 1121
Fragments arranged by size:
1539 1121 993 685 191
SfoI GGC'GCC
Cuts at: 381 450 665 966 1005 1143 1961 2209 381
Size: 69 215 301 39 138 818 248 2701
Fragments arranged by size:
2701 818 301 248 215 138 69 39
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4529
SmaI CCC'GGG
Cuts at: 320 1086 1200 1551 320
Size: 766 114 351 3298
Fragments arranged by size:
3298 766 351 114
SmlI C'TyrA_G
Cuts at: 1684 2599 3091 3353 1684
Size: 915 492 262 2860
Fragments arranged by size:
2860 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4529
SphI G_CATG'C
Cuts at: 363 1582 2082 363
Size: 1219 500 2810
Fragments arranged by size:
2810 1219 500
StuI AGG'CCT
Cuts at: 181 218 181
Size: 37 4492
StyI C'CwwG_G
Cuts at: 324 416 452 527 647 968 1488 1663 2017
Size: 92 36 75 120 321 520 175 354
Cuts at: 2017 324
Size: 2836
Fragments arranged by size:
2836 520 354 321 175 120 92 75
36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 445 2112 2245 2704 2913 445
Size: 1667 133 459 209 2061
Fragments arranged by size:
2061 1667 459 209 133
TatI w'GTAC_w
Cuts at: 4044 4044
Size: 4529
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 757 789 838 870 1291 1323 4046 4078 757
Size: 32 49 32 421 32 2723 32 1208
Fragments arranged by size:
2723 1208 421 49 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2326 2326
Size: 4529
XbaI T'CTAG_A
Cuts at: 1560 1560
Size: 4529
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 483 2024 483
Size: 1541 2988
XmaI C'CCGG_G
Cuts at: 318 1084 1198 1549 318
Size: 766 114 351 3298
Fragments arranged by size:
3298 766 351 114
XmnI GAAnn'nnTTC
Cuts at: 901 901
Size: 4529
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PciI PflMI PmeI PpiI PpuMI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI BaeI
BfrBI BmgBI BmtI BsmI BsmBI BsrGI BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FspAI HindIII
HpaI MfeI NdeI NheI NruI NsiI PacI PasI
PfoI PmlI PshAI PsiI PspXI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI