Restriction map for
pF1KB8363
(Circular) (Six-base) MAPSORT of: pF1KB8363.seq Check: 8818 from: 1 to: 4622
pF1KB8363 4622 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1660 1660
Size: 4622
Acc65I G'GTAC_C
Cuts at: 1202 1638 1202
Size: 436 4186
AclI AA'CG_TT
Cuts at: 4462 4462
Size: 4622
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 2447 2879 3626 3894 182
Size: 2265 432 747 268 910
Fragments arranged by size:
2265 910 747 432 268
AflIII A'CryG_T
Cuts at: 665 1614 1974 3078 4055 665
Size: 949 360 1104 977 1232
Fragments arranged by size:
1232 1104 977 949 360
AgeI A'CCGG_T
Cuts at: 3010 3010
Size: 4622
AhdI GACnn_n'nnGTC
Cuts at: 1038 1038
Size: 4622
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 502 502
Size: 4622
AlwNI CAG_nnn'CTG
Cuts at: 256 326 1030 1927 3494 256
Size: 70 704 897 1567 1384
Fragments arranged by size:
1567 1384 897 704 70
ApaI G_GGCC'C
Cuts at: 1409 1409
Size: 4622
ApaLI G'TGCA_C
Cuts at: 3392 3392
Size: 4622
ApoI r'AATT_y
Cuts at: 355 1342 1356 1626 3975 4231 355
Size: 987 14 270 2349 256 746
Fragments arranged by size:
2349 987 746 270 256 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 4622
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4622
AvaI C'yCGr_G
Cuts at: 1318 1642 1318
Size: 324 4298
BamHI G'GATC_C
Cuts at: 1647 1647
Size: 4622
BanI G'GyrC_C
Cuts at: 1202 1638 2052 2300 2335 1202
Size: 436 414 248 35 3489
Fragments arranged by size:
3489 436 414 248 35
BanII G_rGCy'C
Cuts at: 527 1409 1636 2666 527
Size: 882 227 1030 2483
Fragments arranged by size:
2483 1030 882 227
BbeI G_GCGC'C
Cuts at: 2056 2304 2056
Size: 248 4374
BbsI GAAGACnn'nnnn_
Cuts at: 1495 1495
Size: 4622
BciVI GTATCCnnnnn_n'
Cuts at: 2520 3281 4622 2520
Size: 761 1341 2520
Fragments arranged by size:
2520 1341 761
BclI T'GATC_A
Cuts at: 2142 2142
Size: 4622
BfrBI ATG'CAT
Cuts at: 489 775 489
Size: 286 4336
BglII A'GATC_T
Cuts at: 2137 4174 2137
Size: 2037 2585
BlpI GC'TnA_GC
Cuts at: 1734 1734
Size: 4622
Bme1580I G_kGCm'C
Cuts at: 238 1409 2247 2340 3396 238
Size: 1171 838 93 1056 1464
Fragments arranged by size:
1464 1171 1056 838 93
BmgBI CAC'GTC
Cuts at: 666 666
Size: 4622
BmrI ACTGGGnnnn_n'
Cuts at: 1261 1834 1915 2250 4394 1261
Size: 573 81 335 2144 1489
Fragments arranged by size:
2144 1489 573 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2989 2989
Size: 4622
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 398 398
Size: 4622
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 393 738 1798 2677 3169 3467 393
Size: 345 1060 879 492 298 1548
Fragments arranged by size:
1548 1060 879 492 345 298
BsaI GGTCTCn'nnnn_
Cuts at: 4326 4326
Size: 4622
BsaAI yAC'GTr
Cuts at: 1485 1975 1485
Size: 490 4132
BsaBI GATnn'nnATC
Cuts at: 6 313 2136 4173 6
Size: 307 1823 2037 455
Fragments arranged by size:
2037 1823 455 307
BsaHI Gr'CG_yC
Cuts at: 2053 2301 3003 4499 2053
Size: 248 702 1496 2176
Fragments arranged by size:
2176 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1811 2332 3010 3284 3431 3842 1811
Size: 521 678 274 147 411 2591
Fragments arranged by size:
2591 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 187 649 778 187
Size: 462 129 4031
Fragments arranged by size:
4031 462 129
BseYI C'CCAG_C
Cuts at: 2047 2805 3382 3878 2047
Size: 758 577 496 2791
Fragments arranged by size:
2791 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1439 1439
Size: 4622
BsiEI CG_ry'CG
Cuts at: 104 2164 2210 3418 104
Size: 2060 46 1208 1308
Fragments arranged by size:
2060 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 527 1636 2414 2604 3396 527
Size: 1109 778 190 792 1753
Fragments arranged by size:
1753 1109 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1467 1467
Size: 4622
Bsp1286I G_dGCh'C
Cuts at: 238 527 1409 1636 2247 2340 2414 2604 2666
Size: 289 882 227 611 93 74 190 62
Cuts at: 2666 3396 238
Size: 730 1464
Fragments arranged by size:
1464 882 730 611 289 227 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3842 3842
Size: 4622
BspHI T'CATG_A
Cuts at: 3798 3798
Size: 4622
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1672 1886 2188 2569 1672
Size: 214 302 381 3725
Fragments arranged by size:
3725 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2914 2968 4452 4617 50
Size: 2864 54 1484 165 55
Fragments arranged by size:
2864 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1070 1492 2534 1070
Size: 422 1042 3158
Fragments arranged by size:
3158 1042 422
BsrFI r'CCGG_y
Cuts at: 2620 2801 3010 2620
Size: 181 209 4232
Fragments arranged by size:
4232 209 181
BssSI C'ACGA_G
Cuts at: 2893 3251 2893
Size: 358 4264
BstAPI GCAn_nnn'nTGC
Cuts at: 326 1999 326
Size: 1673 2949
BstBI TT'CG_AA
Cuts at: 2983 2983
Size: 4622
BstEII G'GTnAC_C
Cuts at: 639 973 639
Size: 334 4288
BstXI CCAn_nnnn'nTGG
Cuts at: 114 527 114
Size: 413 4209
BstYI r'GATC_y
Cuts at: 1 371 722 1647 2114 2137 2472 2718 3730
Size: 370 351 925 467 23 335 246 1012
Cuts at: 3730 3816 3828 4174 1
Size: 86 12 346 449
Fragments arranged by size:
1012 925 467 449 370 351 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 291 1410 1436 107
Size: 184 1119 26 3293
Fragments arranged by size:
3293 1119 184 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1416 2572 2753 2916 1416
Size: 1156 181 163 3122
Fragments arranged by size:
3122 1156 181 163
BtsI GCAGTG_nn'
Cuts at: 327 481 1865 327
Size: 154 1384 3084
Fragments arranged by size:
3084 1384 154
ClaI AT'CG_AT
Cuts at: 1256 1256
Size: 4622
DraI TTT'AAA
Cuts at: 1621 1621
Size: 4622
DrdI GACnn_nn'nnGTC
Cuts at: 2328 3186 2328
Size: 858 3764
EaeI y'GGCC_r
Cuts at: 2207 2381 2772 2799 4144 2207
Size: 174 391 27 1345 2685
Fragments arranged by size:
2685 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2207 2207
Size: 4622
EarI CTCTTCn'nnn_
Cuts at: 718 908 1088 2645 2855 718
Size: 190 180 1557 210 2485
Fragments arranged by size:
2485 1557 210 190 180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2830 3140 3286 4432 2830
Size: 310 146 1146 3020
Fragments arranged by size:
3020 1146 310 146
EcoICRI GAG'CTC
Cuts at: 525 1634 525
Size: 1109 3513
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 398 2447 2879 3626 3894 182
Size: 216 2049 432 747 268 910
Fragments arranged by size:
2049 910 747 432 268 216
EcoO109I rG'GnC_Cy
Cuts at: 253 568 1406 1761 253
Size: 315 838 355 3114
Fragments arranged by size:
3114 838 355 315
EcoRI G'AATT_C
Cuts at: 355 1626 355
Size: 1271 3351
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2641 2641
Size: 4622
FspI TGC'GCA
Cuts at: 1231 2403 1231
Size: 1172 3450
HaeII r_GCGC'y
Cuts at: 2056 2304 3326 4305 2056
Size: 248 1022 979 2373
Fragments arranged by size:
2373 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 223 255 269 301 356 388 1011 1024 1043
Size: 32 14 32 55 32 623 13 19
Cuts at: 1043 1056 2464 2494 2496 2526 223
Size: 13 1408 30 2 30 2319
Fragments arranged by size:
2319 1408 623 55 32 32 32 30
30 19 14 13 13 2
HincII GTy'rAC
Cuts at: 671 1661 671
Size: 990 3632
KasI G'GCGC_C
Cuts at: 2052 2300 2052
Size: 248 4374
KpnI G_GTAC'C
Cuts at: 1206 1642 1206
Size: 436 4186
MluI A'CGCG_T
Cuts at: 4055 4055
Size: 4622
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 611 3293 3477 611
Size: 2682 184 1756
Fragments arranged by size:
2682 1756 184
MscI TGG'CCA
Cuts at: 2383 2383
Size: 4622
MslI CAynn'nnrTG
Cuts at: 484 1085 1435 1441 1550 2611 2738 484
Size: 601 350 6 109 1061 127 2368
Fragments arranged by size:
2368 1061 601 350 127 109 6
MspA1I CmG'CkG
Cuts at: 1733 2047 2407 3420 3665 1733
Size: 314 360 1013 245 2690
Fragments arranged by size:
2690 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2803 2803
Size: 4622
NarI GG'CG_CC
Cuts at: 2053 2301 2053
Size: 248 4374
NcoI C'CATG_G
Cuts at: 107 1436 107
Size: 1329 3293
NgoMIV G'CCGG_C
Cuts at: 2801 2801
Size: 4622
NsiI A_TGCA'T
Cuts at: 491 777 491
Size: 286 4336
NspI r_CATG'y
Cuts at: 1200 1569 1675 2175 3082 1200
Size: 369 106 500 907 2740
Fragments arranged by size:
2740 907 500 369 106
PciI A'CATG_T
Cuts at: 3078 3078
Size: 4622
PflMI CCAn_nnn'nTGG
Cuts at: 256 256
Size: 4622
PmeI GTTT'AAAC
Cuts at: 1621 1621
Size: 4622
PmlI CAC'GTG
Cuts at: 1485 1485
Size: 4622
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 3761
Size: 32 4590
PpuMI rG'GwC_Cy
Cuts at: 253 253
Size: 4622
PshAI GACnn'nnGTC
Cuts at: 1604 1604
Size: 4622
PsiI TTA'TAA
Cuts at: 140 140
Size: 4622
PspOMI G'GGCC_C
Cuts at: 1405 1405
Size: 4622
PspXI vC'TCGA_Gb
Cuts at: 1318 1318
Size: 4622
PstI C_TGCA'G
Cuts at: 476 1669 2354 476
Size: 1193 685 2744
Fragments arranged by size:
2744 1193 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4622
PvuII CAG'CTG
Cuts at: 2047 2407 2047
Size: 360 4262
RsrII CG'GwC_CG
Cuts at: 2817 2817
Size: 4622
SacI G_AGCT'C
Cuts at: 527 1636 527
Size: 1109 3513
SalI G'TCGA_C
Cuts at: 1659 1659
Size: 4622
SapI GCTCTTCn'nnn_
Cuts at: 718 2645 2855 718
Size: 1927 210 2485
Fragments arranged by size:
2485 1927 210
SbfI CC_TGCA'GG
Cuts at: 1669 1669
Size: 4622
ScaI AGT'ACT
Cuts at: 4139 4139
Size: 4622
SexAI A'CCwGG_T
Cuts at: 249 249
Size: 4622
SfcI C'TryA_G
Cuts at: 33 472 1665 2350 3343 3534 33
Size: 439 1193 685 993 191 1121
Fragments arranged by size:
1193 1121 993 685 439 191
SfoI GGC'GCC
Cuts at: 2054 2302 2054
Size: 248 4374
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4622
SmaI CCC'GGG
Cuts at: 1644 1644
Size: 4622
SmlI C'TyrA_G
Cuts at: 408 717 1318 1777 2692 3184 3446 408
Size: 309 601 459 915 492 262 1584
Fragments arranged by size:
1584 915 601 492 459 309 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4622
SphI G_CATG'C
Cuts at: 1200 1675 2175 1200
Size: 475 500 3647
Fragments arranged by size:
3647 500 475
SspI AAT'ATT
Cuts at: 1299 1299
Size: 4622
StyI C'CwwG_G
Cuts at: 107 759 1436 1756 2110 107
Size: 652 677 320 354 2619
Fragments arranged by size:
2619 677 652 354 320
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1562 2205 2338 2797 3006 1562
Size: 643 133 459 209 3178
Fragments arranged by size:
3178 643 459 209 133
TatI w'GTAC_w
Cuts at: 1427 4137 1427
Size: 2710 1912
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 306 338 1522 1554 4139 4171 306
Size: 32 1184 32 2585 32 757
Fragments arranged by size:
2585 1184 757 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1222 2419 1222
Size: 1197 3425
XbaI T'CTAG_A
Cuts at: 1653 1653
Size: 4622
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1123 2117 1123
Size: 994 3628
XhoI C'TCGA_G
Cuts at: 1318 1318
Size: 4622
XmaI C'CCGG_G
Cuts at: 1642 1642
Size: 4622
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI AscI AvrII
BaeI BbvCI BcgI BglI BmtI Bpu10I BsaXI BsiWI
BsmI BsrGI BssHII BstZ17I Bsu36I CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PasI PfoI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI StuI SwaI XmnI ZraI