Restriction map for
pF1KB8360
(Circular) (Six-base) MAPSORT of: pF1KB8360.seq Check: 8662 from: 1 to: 3617
pF1KB8360 3617 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 655 655
Size: 3617
Acc65I G'GTAC_C
Cuts at: 633 633
Size: 3617
AclI AA'CG_TT
Cuts at: 3457 3457
Size: 3617
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 472 493 1442 1874 2621 2889 218
Size: 254 21 949 432 747 268 946
Fragments arranged by size:
949 946 747 432 268 254 21
AflIII A'CryG_T
Cuts at: 969 2073 3050 969
Size: 1104 977 1536
Fragments arranged by size:
1536 1104 977
AgeI A'CCGG_T
Cuts at: 2005 2005
Size: 3617
AlwNI CAG_nnn'CTG
Cuts at: 922 2489 922
Size: 1567 2050
ApaLI G'TGCA_C
Cuts at: 2387 2387
Size: 3617
ApoI r'AATT_y
Cuts at: 142 621 2970 3226 142
Size: 479 2349 256 533
Fragments arranged by size:
2349 533 479 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3617
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3617
AvaI C'yCGr_G
Cuts at: 637 637
Size: 3617
BamHI G'GATC_C
Cuts at: 642 642
Size: 3617
BanI G'GyrC_C
Cuts at: 633 1047 1295 1330 633
Size: 414 248 35 2920
Fragments arranged by size:
2920 414 248 35
BanII G_rGCy'C
Cuts at: 483 631 1661 483
Size: 148 1030 2439
Fragments arranged by size:
2439 1030 148
BbeI G_GCGC'C
Cuts at: 1051 1299 1051
Size: 248 3369
BbsI GAAGACnn'nnnn_
Cuts at: 355 460 511 355
Size: 105 51 3461
Fragments arranged by size:
3461 105 51
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 118 124 152 90
Size: 28 6 28 3555
Fragments arranged by size:
3555 28 28 6
BciVI GTATCCnnnnn_n'
Cuts at: 183 1515 2276 3617 183
Size: 1332 761 1341 183
Fragments arranged by size:
1341 1332 761 183
BclI T'GATC_A
Cuts at: 1137 1137
Size: 3617
BglI GCCn_nnn'nGGC
Cuts at: 112 160 112
Size: 48 3569
BglII A'GATC_T
Cuts at: 1132 3169 1132
Size: 2037 1580
BlpI GC'TnA_GC
Cuts at: 729 729
Size: 3617
Bme1580I G_kGCm'C
Cuts at: 278 304 316 1242 1335 2391 278
Size: 26 12 926 93 1056 1504
Fragments arranged by size:
1504 1056 926 93 26 12
BmrI ACTGGGnnnn_n'
Cuts at: 829 910 1245 3389 829
Size: 81 335 2144 1057
Fragments arranged by size:
2144 1057 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1984 1984
Size: 3617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 344 344
Size: 3617
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 539 793 1672 2164 2462 539
Size: 254 879 492 298 1694
Fragments arranged by size:
1694 879 492 298 254
BsaI GGTCTCn'nnnn_
Cuts at: 3321 3321
Size: 3617
BsaAI yAC'GTr
Cuts at: 970 970
Size: 3617
BsaBI GATnn'nnATC
Cuts at: 6 1131 3168 6
Size: 1125 2037 455
Fragments arranged by size:
2037 1125 455
BsaHI Gr'CG_yC
Cuts at: 1048 1296 1998 3494 1048
Size: 248 702 1496 1171
Fragments arranged by size:
1496 1171 702 248
BsaWI w'CCGG_w
Cuts at: 806 1327 2005 2279 2426 2837 806
Size: 521 678 274 147 411 1586
Fragments arranged by size:
1586 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 255 285 255
Size: 30 3587
BseYI C'CCAG_C
Cuts at: 297 1042 1800 2377 2873 297
Size: 745 758 577 496 1041
Fragments arranged by size:
1041 758 745 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 469
Size: 3617
BsiEI CG_ry'CG
Cuts at: 104 1159 1205 2413 104
Size: 1055 46 1208 1308
Fragments arranged by size:
1308 1208 1055 46
BsiHKAI G_wGCw'C
Cuts at: 631 1409 1599 2391 631
Size: 778 190 792 1857
Fragments arranged by size:
1857 792 778 190
BsmI GAATG_Cn'
Cuts at: 163 502 163
Size: 339 3278
Bsp1286I G_dGCh'C
Cuts at: 278 304 316 483 631 1242 1335 1409 1599
Size: 26 12 167 148 611 93 74 190
Cuts at: 1599 1661 2391 278
Size: 62 730 1504
Fragments arranged by size:
1504 730 611 190 167 148 93 74
62 26 12
BspEI T'CCGG_A
Cuts at: 2837 2837
Size: 3617
BspHI T'CATG_A
Cuts at: 2793 2793
Size: 3617
BspMI ACCTGCnnnn'nnnn_
Cuts at: 667 881 1183 1564 667
Size: 214 302 381 2720
Fragments arranged by size:
2720 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1909 1963 3447 3612 50
Size: 1859 54 1484 165 55
Fragments arranged by size:
1859 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1529 1529
Size: 3617
BsrFI r'CCGG_y
Cuts at: 1615 1796 2005 1615
Size: 181 209 3227
Fragments arranged by size:
3227 209 181
BssSI C'ACGA_G
Cuts at: 1888 2246 1888
Size: 358 3259
BstAPI GCAn_nnn'nTGC
Cuts at: 994 994
Size: 3617
BstBI TT'CG_AA
Cuts at: 1978 1978
Size: 3617
BstYI r'GATC_y
Cuts at: 1 365 642 1109 1132 1467 1713 2725 2811
Size: 364 277 467 23 335 246 1012 86
Cuts at: 2811 2823 3169 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 364 346 335 277 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1567 1748 1911 1567
Size: 181 163 3273
Fragments arranged by size:
3273 181 163
BtsI GCAGTG_nn'
Cuts at: 265 860 265
Size: 595 3022
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 510 545 510
Size: 35 3582
DraI TTT'AAA
Cuts at: 616 616
Size: 3617
DrdI GACnn_nn'nnGTC
Cuts at: 1323 2181 1323
Size: 858 2759
EaeI y'GGCC_r
Cuts at: 1202 1376 1767 1794 3139 1202
Size: 174 391 27 1345 1680
Fragments arranged by size:
1680 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1202 1202
Size: 3617
EarI CTCTTCn'nnn_
Cuts at: 1640 1850 1640
Size: 210 3407
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1825 2135 2281 3427 1825
Size: 310 146 1146 2015
Fragments arranged by size:
2015 1146 310 146
EcoICRI GAG'CTC
Cuts at: 629 629
Size: 3617
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 344 472 493 1442 1874 2621 2889 218
Size: 126 128 21 949 432 747 268 946
Fragments arranged by size:
949 946 747 432 268 128 126 21
EcoO109I rG'GnC_Cy
Cuts at: 756 756
Size: 3617
EcoRI G'AATT_C
Cuts at: 621 621
Size: 3617
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1636 1636
Size: 3617
FspI TGC'GCA
Cuts at: 1398 1398
Size: 3617
HaeII r_GCGC'y
Cuts at: 1051 1299 2321 3300 1051
Size: 248 1022 979 1368
Fragments arranged by size:
1368 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 350 382 1459 1489 1491 1521 350
Size: 32 1077 30 2 30 2446
Fragments arranged by size:
2446 1077 32 30 30 2
HincII GTy'rAC
Cuts at: 656 656
Size: 3617
KasI G'GCGC_C
Cuts at: 1047 1295 1047
Size: 248 3369
KpnI G_GTAC'C
Cuts at: 637 637
Size: 3617
MluI A'CGCG_T
Cuts at: 3050 3050
Size: 3617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2288 2472 2288
Size: 184 3433
MscI TGG'CCA
Cuts at: 1378 1378
Size: 3617
MslI CAynn'nnrTG
Cuts at: 1606 1733 1606
Size: 127 3490
MspA1I CmG'CkG
Cuts at: 151 184 728 1042 1402 2415 2660 151
Size: 33 544 314 360 1013 245 1108
Fragments arranged by size:
1108 1013 544 360 314 245 33
NaeI GCC'GGC
Cuts at: 1798 1798
Size: 3617
NarI GG'CG_CC
Cuts at: 1048 1296 1048
Size: 248 3369
NgoMIV G'CCGG_C
Cuts at: 1796 1796
Size: 3617
NspI r_CATG'y
Cuts at: 670 1170 2077 670
Size: 500 907 2210
Fragments arranged by size:
2210 907 500
PciI A'CATG_T
Cuts at: 2073 2073
Size: 3617
PmeI GTTT'AAAC
Cuts at: 616 616
Size: 3617
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2756 2788 2756
Size: 32 3585
PstI C_TGCA'G
Cuts at: 664 1349 664
Size: 685 2932
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3617
PvuII CAG'CTG
Cuts at: 151 184 1042 1402 151
Size: 33 858 360 2366
Fragments arranged by size:
2366 858 360 33
RsrII CG'GwC_CG
Cuts at: 1812 1812
Size: 3617
SacI G_AGCT'C
Cuts at: 631 631
Size: 3617
SalI G'TCGA_C
Cuts at: 654 654
Size: 3617
SapI GCTCTTCn'nnn_
Cuts at: 1640 1850 1640
Size: 210 3407
SbfI CC_TGCA'GG
Cuts at: 664 664
Size: 3617
ScaI AGT'ACT
Cuts at: 3134 3134
Size: 3617
SfcI C'TryA_G
Cuts at: 33 660 1345 2338 2529 33
Size: 627 685 993 191 1121
Fragments arranged by size:
1121 993 685 627 191
SfoI GGC'GCC
Cuts at: 1049 1297 1049
Size: 248 3369
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3617
SmaI CCC'GGG
Cuts at: 639 639
Size: 3617
SmlI C'TyrA_G
Cuts at: 554 772 1687 2179 2441 554
Size: 218 915 492 262 1730
Fragments arranged by size:
1730 915 492 262 218
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3617
SphI G_CATG'C
Cuts at: 670 1170 670
Size: 500 3117
StuI AGG'CCT
Cuts at: 421 421
Size: 3617
StyI C'CwwG_G
Cuts at: 751 1105 751
Size: 354 3263
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1200 1333 1792 2001 1200
Size: 133 459 209 2816
Fragments arranged by size:
2816 459 209 133
TatI w'GTAC_w
Cuts at: 3132 3132
Size: 3617
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 255 287 3134 3166 255
Size: 32 2847 32 706
Fragments arranged by size:
2847 706 32 32
Tth111I GACn'n_nGTC
Cuts at: 1414 1414
Size: 3617
XbaI T'CTAG_A
Cuts at: 648 648
Size: 3617
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1112 1112
Size: 3617
XmaI C'CCGG_G
Cuts at: 637 637
Size: 3617
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI
Bpu10I BseRI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI