Restriction map for
pF1KB8359
(Circular) (Six-base) MAPSORT of: pF1KB8359.seq Check: 6728 from: 1 to: 3470
pF1KB8359 3470 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 508 508
Size: 3470
Acc65I G'GTAC_C
Cuts at: 486 486
Size: 3470
AclI AA'CG_TT
Cuts at: 3310 3310
Size: 3470
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1295 1727 2474 2742 1295
Size: 432 747 268 2023
Fragments arranged by size:
2023 747 432 268
AflIII A'CryG_T
Cuts at: 822 1926 2903 822
Size: 1104 977 1389
Fragments arranged by size:
1389 1104 977
AgeI A'CCGG_T
Cuts at: 1858 1858
Size: 3470
AlwNI CAG_nnn'CTG
Cuts at: 378 775 2342 378
Size: 397 1567 1506
Fragments arranged by size:
1567 1506 397
ApaLI G'TGCA_C
Cuts at: 2240 2240
Size: 3470
ApoI r'AATT_y
Cuts at: 474 2823 3079 474
Size: 2349 256 865
Fragments arranged by size:
2349 865 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3470
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3470
AvaI C'yCGr_G
Cuts at: 348 490 348
Size: 142 3328
BamHI G'GATC_C
Cuts at: 495 495
Size: 3470
BanI G'GyrC_C
Cuts at: 486 900 1148 1183 486
Size: 414 248 35 2773
Fragments arranged by size:
2773 414 248 35
BanII G_rGCy'C
Cuts at: 424 484 1514 424
Size: 60 1030 2380
Fragments arranged by size:
2380 1030 60
BbeI G_GCGC'C
Cuts at: 904 1152 904
Size: 248 3222
BciVI GTATCCnnnnn_n'
Cuts at: 1368 2129 3470 1368
Size: 761 1341 1368
Fragments arranged by size:
1368 1341 761
BclI T'GATC_A
Cuts at: 990 990
Size: 3470
BglI GCCn_nnn'nGGC
Cuts at: 112 442 112
Size: 330 3140
BglII A'GATC_T
Cuts at: 985 3022 985
Size: 2037 1433
BlpI GC'TnA_GC
Cuts at: 582 582
Size: 3470
Bme1580I G_kGCm'C
Cuts at: 242 1095 1188 2244 242
Size: 853 93 1056 1468
Fragments arranged by size:
1468 1056 853 93
BmrI ACTGGGnnnn_n'
Cuts at: 388 682 763 1098 3242 388
Size: 294 81 335 2144 616
Fragments arranged by size:
2144 616 335 294 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1837 1837
Size: 3470
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 1525 2017 2315 646
Size: 879 492 298 1801
Fragments arranged by size:
1801 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3174 3174
Size: 3470
BsaAI yAC'GTr
Cuts at: 823 823
Size: 3470
BsaBI GATnn'nnATC
Cuts at: 6 984 3021 6
Size: 978 2037 455
Fragments arranged by size:
2037 978 455
BsaHI Gr'CG_yC
Cuts at: 901 1149 1851 3347 901
Size: 248 702 1496 1024
Fragments arranged by size:
1496 1024 702 248
BsaWI w'CCGG_w
Cuts at: 397 659 1180 1858 2132 2279 2690 397
Size: 262 521 678 274 147 411 1177
Fragments arranged by size:
1177 678 521 411 274 262 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 215 245 215
Size: 30 3440
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 239 239
Size: 3470
BseYI C'CCAG_C
Cuts at: 895 1653 2230 2726 895
Size: 758 577 496 1639
Fragments arranged by size:
1639 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1012 1058 2266 104
Size: 908 46 1208 1308
Fragments arranged by size:
1308 1208 908 46
BsiHKAI G_wGCw'C
Cuts at: 251 484 1262 1452 2244 251
Size: 233 778 190 792 1477
Fragments arranged by size:
1477 792 778 233 190
Bsp1286I G_dGCh'C
Cuts at: 242 251 424 484 1095 1188 1262 1452 1514
Size: 9 173 60 611 93 74 190 62
Cuts at: 1514 2244 242
Size: 730 1468
Fragments arranged by size:
1468 730 611 190 173 93 74 62
60 9
BspEI T'CCGG_A
Cuts at: 397 2690 397
Size: 2293 1177
BspHI T'CATG_A
Cuts at: 2646 2646
Size: 3470
BspMI ACCTGCnnnn'nnnn_
Cuts at: 520 734 1036 1417 520
Size: 214 302 381 2573
Fragments arranged by size:
2573 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1762 1816 3300 3465 50
Size: 1712 54 1484 165 55
Fragments arranged by size:
1712 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 323 437 449 1382 323
Size: 114 12 933 2411
Fragments arranged by size:
2411 933 114 12
BsrFI r'CCGG_y
Cuts at: 1468 1649 1858 1468
Size: 181 209 3080
Fragments arranged by size:
3080 209 181
BssSI C'ACGA_G
Cuts at: 1741 2099 1741
Size: 358 3112
BstAPI GCAn_nnn'nTGC
Cuts at: 847 847
Size: 3470
BstBI TT'CG_AA
Cuts at: 1831 1831
Size: 3470
BstYI r'GATC_y
Cuts at: 1 495 962 985 1320 1566 2578 2664 2676
Size: 494 467 23 335 246 1012 86 12
Cuts at: 2676 3022 1
Size: 346 449
Fragments arranged by size:
1012 494 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 242 257 314 107
Size: 135 15 57 3263
Fragments arranged by size:
3263 135 57 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1420 1601 1764 1420
Size: 181 163 3126
Fragments arranged by size:
3126 181 163
BtsI GCAGTG_nn'
Cuts at: 143 266 713 143
Size: 123 447 2900
Fragments arranged by size:
2900 447 123
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 318 353 318
Size: 35 3435
DraI TTT'AAA
Cuts at: 469 469
Size: 3470
DrdI GACnn_nn'nnGTC
Cuts at: 1176 2034 1176
Size: 858 2612
EaeI y'GGCC_r
Cuts at: 149 197 320 1055 1229 1620 1647 2992 149
Size: 48 123 735 174 391 27 1345 627
Fragments arranged by size:
1345 735 627 391 174 123 48 27
EagI C'GGCC_G
Cuts at: 1055 1055
Size: 3470
EarI CTCTTCn'nnn_
Cuts at: 1493 1703 1493
Size: 210 3260
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1678 1988 2134 3280 1678
Size: 310 146 1146 1868
Fragments arranged by size:
1868 1146 310 146
EcoICRI GAG'CTC
Cuts at: 482 482
Size: 3470
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1295 1727 2474 2742 1295
Size: 432 747 268 2023
Fragments arranged by size:
2023 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 609 609
Size: 3470
EcoRI G'AATT_C
Cuts at: 474 474
Size: 3470
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1489 1489
Size: 3470
FspI TGC'GCA
Cuts at: 1251 1251
Size: 3470
HaeII r_GCGC'y
Cuts at: 904 1152 2174 3153 904
Size: 248 1022 979 1221
Fragments arranged by size:
1221 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1312 1342 1344 1374 1312
Size: 30 2 30 3408
Fragments arranged by size:
3408 30 30 2
HincII GTy'rAC
Cuts at: 509 509
Size: 3470
KasI G'GCGC_C
Cuts at: 900 1148 900
Size: 248 3222
KpnI G_GTAC'C
Cuts at: 490 490
Size: 3470
MluI A'CGCG_T
Cuts at: 2903 2903
Size: 3470
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2141 2325 2141
Size: 184 3286
MscI TGG'CCA
Cuts at: 151 199 1231 151
Size: 48 1032 2390
Fragments arranged by size:
2390 1032 48
MslI CAynn'nnrTG
Cuts at: 1459 1586 1459
Size: 127 3343
MspA1I CmG'CkG
Cuts at: 316 581 895 1255 2268 2513 316
Size: 265 314 360 1013 245 1273
Fragments arranged by size:
1273 1013 360 314 265 245
NaeI GCC'GGC
Cuts at: 1651 1651
Size: 3470
NarI GG'CG_CC
Cuts at: 901 1149 901
Size: 248 3222
NcoI C'CATG_G
Cuts at: 107 242 257 107
Size: 135 15 3320
Fragments arranged by size:
3320 135 15
NgoMIV G'CCGG_C
Cuts at: 1649 1649
Size: 3470
NspI r_CATG'y
Cuts at: 523 1023 1930 523
Size: 500 907 2063
Fragments arranged by size:
2063 907 500
PciI A'CATG_T
Cuts at: 1926 1926
Size: 3470
PmeI GTTT'AAAC
Cuts at: 469 469
Size: 3470
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2609 2641 2609
Size: 32 3438
PstI C_TGCA'G
Cuts at: 374 517 1202 374
Size: 143 685 2642
Fragments arranged by size:
2642 685 143
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3470
PvuII CAG'CTG
Cuts at: 895 1255 895
Size: 360 3110
RsrII CG'GwC_CG
Cuts at: 1665 1665
Size: 3470
SacI G_AGCT'C
Cuts at: 484 484
Size: 3470
SacII CC_GC'GG
Cuts at: 317 317
Size: 3470
SalI G'TCGA_C
Cuts at: 507 507
Size: 3470
SapI GCTCTTCn'nnn_
Cuts at: 1493 1703 1493
Size: 210 3260
SbfI CC_TGCA'GG
Cuts at: 517 517
Size: 3470
ScaI AGT'ACT
Cuts at: 2987 2987
Size: 3470
SfcI C'TryA_G
Cuts at: 33 209 370 513 1198 2191 2382 33
Size: 176 161 143 685 993 191 1121
Fragments arranged by size:
1121 993 685 191 176 161 143
SfoI GGC'GCC
Cuts at: 902 1150 902
Size: 248 3222
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3470
SmaI CCC'GGG
Cuts at: 492 492
Size: 3470
SmlI C'TyrA_G
Cuts at: 625 1540 2032 2294 625
Size: 915 492 262 1801
Fragments arranged by size:
1801 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3470
SphI G_CATG'C
Cuts at: 523 1023 523
Size: 500 2970
StuI AGG'CCT
Cuts at: 115 115
Size: 3470
StyI C'CwwG_G
Cuts at: 107 242 257 604 958 107
Size: 135 15 347 354 2619
Fragments arranged by size:
2619 354 347 135 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1053 1186 1645 1854 1053
Size: 133 459 209 2669
Fragments arranged by size:
2669 459 209 133
TatI w'GTAC_w
Cuts at: 2985 2985
Size: 3470
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 215 247 2987 3019 215
Size: 32 2740 32 666
Fragments arranged by size:
2740 666 32 32
Tth111I GACn'n_nGTC
Cuts at: 1267 1267
Size: 3470
XbaI T'CTAG_A
Cuts at: 501 501
Size: 3470
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 414 965 414
Size: 551 2919
XmaI C'CCGG_G
Cuts at: 490 490
Size: 3470
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI BpmI Bpu10I BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI