Restriction map for
pF1KB8356
(Circular) (Six-base) MAPSORT of: pF1KB8356.seq Check: 8960 from: 1 to: 4256
pF1KB8356 4256 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 726 726
Size: 4256
AccI GT'mk_AC
Cuts at: 674 1187 1294 674
Size: 513 107 3636
Fragments arranged by size:
3636 513 107
Acc65I G'GTAC_C
Cuts at: 1272 1272
Size: 4256
AclI AA'CG_TT
Cuts at: 4096 4096
Size: 4256
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2081 2513 3260 3528 2081
Size: 432 747 268 2809
Fragments arranged by size:
2809 747 432 268
AflIII A'CryG_T
Cuts at: 1043 1608 2712 3689 1043
Size: 565 1104 977 1610
Fragments arranged by size:
1610 1104 977 565
AgeI A'CCGG_T
Cuts at: 2644 2644
Size: 4256
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1177 1177
Size: 4256
AlwNI CAG_nnn'CTG
Cuts at: 1561 3128 1561
Size: 1567 2689
ApaI G_GGCC'C
Cuts at: 142 142
Size: 4256
ApaLI G'TGCA_C
Cuts at: 3026 3026
Size: 4256
ApoI r'AATT_y
Cuts at: 1260 3609 3865 1260
Size: 2349 256 1651
Fragments arranged by size:
2349 1651 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4256
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4256
AvaI C'yCGr_G
Cuts at: 197 718 1276 197
Size: 521 558 3177
Fragments arranged by size:
3177 558 521
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1116 1149 1116
Size: 33 4223
BamHI G'GATC_C
Cuts at: 1281 1281
Size: 4256
BanI G'GyrC_C
Cuts at: 1272 1686 1934 1969 1272
Size: 414 248 35 3559
Fragments arranged by size:
3559 414 248 35
BanII G_rGCy'C
Cuts at: 142 653 1270 2300 142
Size: 511 617 1030 2098
Fragments arranged by size:
2098 1030 617 511
BbeI G_GCGC'C
Cuts at: 1690 1938 1690
Size: 248 4008
BbvCI CC'TCA_GC
Cuts at: 487 707 1013 487
Size: 220 306 3730
Fragments arranged by size:
3730 306 220
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 490 524 490
Size: 34 4222
BciVI GTATCCnnnnn_n'
Cuts at: 2154 2915 4256 2154
Size: 761 1341 2154
Fragments arranged by size:
2154 1341 761
BclI T'GATC_A
Cuts at: 1776 1776
Size: 4256
BglI GCCn_nnn'nGGC
Cuts at: 833 833
Size: 4256
BglII A'GATC_T
Cuts at: 1110 1771 3808 1110
Size: 661 2037 1558
Fragments arranged by size:
2037 1558 661
BlpI GC'TnA_GC
Cuts at: 1368 1368
Size: 4256
Bme1580I G_kGCm'C
Cuts at: 142 342 1881 1974 3030 142
Size: 200 1539 93 1056 1368
Fragments arranged by size:
1539 1368 1056 200 93
BmgBI CAC'GTC
Cuts at: 477 477
Size: 4256
BmrI ACTGGGnnnn_n'
Cuts at: 823 1468 1549 1884 4028 823
Size: 645 81 335 2144 1051
Fragments arranged by size:
2144 1051 645 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2623 2623
Size: 4256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 648 648
Size: 4256
Bpu10I CC'TnA_GC
Cuts at: 487 707 1013 487
Size: 220 306 3730
Fragments arranged by size:
3730 306 220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 272 1231 1432 2311 2803 3101 272
Size: 959 201 879 492 298 1427
Fragments arranged by size:
1427 959 879 492 298 201
BsaI GGTCTCn'nnnn_
Cuts at: 3960 3960
Size: 4256
BsaAI yAC'GTr
Cuts at: 1609 1609
Size: 4256
BsaBI GATnn'nnATC
Cuts at: 6 1770 3807 6
Size: 1764 2037 455
Fragments arranged by size:
2037 1764 455
BsaHI Gr'CG_yC
Cuts at: 1687 1935 2637 4133 1687
Size: 248 702 1496 1810
Fragments arranged by size:
1810 1496 702 248
BsaWI w'CCGG_w
Cuts at: 158 1445 1966 2644 2918 3065 3476 158
Size: 1287 521 678 274 147 411 938
Fragments arranged by size:
1287 938 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 461 491 579 609 722 752 461
Size: 30 88 30 113 30 3965
Fragments arranged by size:
3965 113 88 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 482 600 661 675 713 724 1030 482
Size: 118 61 14 38 11 306 3708
Fragments arranged by size:
3708 306 118 61 38 14 11
BseYI C'CCAG_C
Cuts at: 141 1681 2439 3016 3512 141
Size: 1540 758 577 496 885
Fragments arranged by size:
1540 885 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 1044 754
Size: 290 3966
BsiEI CG_ry'CG
Cuts at: 104 1798 1844 3052 104
Size: 1694 46 1208 1308
Fragments arranged by size:
1694 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1022 1270 2048 2238 3030 1022
Size: 248 778 190 792 2248
Fragments arranged by size:
2248 792 778 248 190
BsmI GAATG_Cn'
Cuts at: 1154 1154
Size: 4256
Bsp1286I G_dGCh'C
Cuts at: 142 342 653 1022 1270 1881 1974 2048 2238
Size: 200 311 369 248 611 93 74 190
Cuts at: 2238 2300 3030 142
Size: 62 730 1368
Fragments arranged by size:
1368 730 611 369 311 248 200 190
93 74 62
BspEI T'CCGG_A
Cuts at: 158 3476 158
Size: 3318 938
BspHI T'CATG_A
Cuts at: 3432 3432
Size: 4256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 726 1306 1520 1822 2203 726
Size: 580 214 302 381 2779
Fragments arranged by size:
2779 580 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2548 2602 4086 4251 50
Size: 2498 54 1484 165 55
Fragments arranged by size:
2498 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2168 2168
Size: 4256
BsrFI r'CCGG_y
Cuts at: 2254 2435 2644 2254
Size: 181 209 3866
Fragments arranged by size:
3866 209 181
BssSI C'ACGA_G
Cuts at: 2527 2885 2527
Size: 358 3898
BstAPI GCAn_nnn'nTGC
Cuts at: 1633 1633
Size: 4256
BstBI TT'CG_AA
Cuts at: 2617 2617
Size: 4256
BstXI CCAn_nnnn'nTGG
Cuts at: 444 444
Size: 4256
BstYI r'GATC_y
Cuts at: 1 1091 1110 1281 1748 1771 2106 2352 3364
Size: 1090 19 171 467 23 335 246 1012
Cuts at: 3364 3450 3462 3808 1
Size: 86 12 346 449
Fragments arranged by size:
1090 1012 467 449 346 335 246 171
86 23 19 12
Bsu36I CC'TnA_GG
Cuts at: 404 404
Size: 4256
BtgI C'CryG_G
Cuts at: 107 567 107
Size: 460 3796
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2206 2387 2550 2206
Size: 181 163 3912
Fragments arranged by size:
3912 181 163
BtsI GCAGTG_nn'
Cuts at: 217 865 1499 217
Size: 648 634 2974
Fragments arranged by size:
2974 648 634
DraI TTT'AAA
Cuts at: 1255 1255
Size: 4256
DrdI GACnn_nn'nnGTC
Cuts at: 1962 2820 1962
Size: 858 3398
EaeI y'GGCC_r
Cuts at: 926 966 1841 2015 2406 2433 3778 926
Size: 40 875 174 391 27 1345 1404
Fragments arranged by size:
1404 1345 875 391 174 40 27
EagI C'GGCC_G
Cuts at: 1841 1841
Size: 4256
EarI CTCTTCn'nnn_
Cuts at: 902 2279 2489 902
Size: 1377 210 2669
Fragments arranged by size:
2669 1377 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2464 2774 2920 4066 2464
Size: 310 146 1146 2654
Fragments arranged by size:
2654 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1268 1268
Size: 4256
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 648 2081 2513 3260 3528 648
Size: 1433 432 747 268 1376
Fragments arranged by size:
1433 1376 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 138 272 1395 138
Size: 134 1123 2999
Fragments arranged by size:
2999 1123 134
EcoRI G'AATT_C
Cuts at: 1260 1260
Size: 4256
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 780 2275 780
Size: 1495 2761
FspI TGC'GCA
Cuts at: 2037 2037
Size: 4256
HaeII r_GCGC'y
Cuts at: 155 762 1690 1938 2960 3939 155
Size: 607 928 248 1022 979 472
Fragments arranged by size:
1022 979 928 607 472 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2098 2128 2130 2160 2098
Size: 30 2 30 4194
Fragments arranged by size:
4194 30 30 2
HincII GTy'rAC
Cuts at: 1295 1295
Size: 4256
KasI G'GCGC_C
Cuts at: 1686 1934 1686
Size: 248 4008
KpnI G_GTAC'C
Cuts at: 1276 1276
Size: 4256
MluI A'CGCG_T
Cuts at: 3689 3689
Size: 4256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2927 3111 2927
Size: 184 4072
MscI TGG'CCA
Cuts at: 928 2017 928
Size: 1089 3167
MslI CAynn'nnrTG
Cuts at: 377 1159 2245 2372 377
Size: 782 1086 127 2261
Fragments arranged by size:
2261 1086 782 127
MspA1I CmG'CkG
Cuts at: 689 1367 1681 2041 3054 3299 689
Size: 678 314 360 1013 245 1646
Fragments arranged by size:
1646 1013 678 360 314 245
NaeI GCC'GGC
Cuts at: 2437 2437
Size: 4256
NarI GG'CG_CC
Cuts at: 1687 1935 1687
Size: 248 4008
NcoI C'CATG_G
Cuts at: 107 107
Size: 4256
NgoMIV G'CCGG_C
Cuts at: 2435 2435
Size: 4256
NspI r_CATG'y
Cuts at: 1047 1309 1809 2716 1047
Size: 262 500 907 2587
Fragments arranged by size:
2587 907 500 262
PciI A'CATG_T
Cuts at: 1043 2712 1043
Size: 1669 2587
PflMI CCAn_nnn'nTGG
Cuts at: 626 626
Size: 4256
PmeI GTTT'AAAC
Cuts at: 1255 1255
Size: 4256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3395 3427 3395
Size: 32 4224
PpuMI rG'GwC_Cy
Cuts at: 272 272
Size: 4256
PspOMI G'GGCC_C
Cuts at: 138 138
Size: 4256
PstI C_TGCA'G
Cuts at: 1303 1988 1303
Size: 685 3571
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4256
PvuII CAG'CTG
Cuts at: 1681 2041 1681
Size: 360 3896
RsrII CG'GwC_CG
Cuts at: 2451 2451
Size: 4256
SacI G_AGCT'C
Cuts at: 1270 1270
Size: 4256
SalI G'TCGA_C
Cuts at: 1293 1293
Size: 4256
SanDI GG'GwC_CC
Cuts at: 272 272
Size: 4256
SapI GCTCTTCn'nnn_
Cuts at: 2279 2489 2279
Size: 210 4046
SbfI CC_TGCA'GG
Cuts at: 1303 1303
Size: 4256
ScaI AGT'ACT
Cuts at: 3773 3773
Size: 4256
SexAI A'CCwGG_T
Cuts at: 602 602
Size: 4256
SfcI C'TryA_G
Cuts at: 33 251 1026 1299 1984 2977 3168 33
Size: 218 775 273 685 993 191 1121
Fragments arranged by size:
1121 993 775 685 273 218 191
SfoI GGC'GCC
Cuts at: 1688 1936 1688
Size: 248 4008
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4256
SmaI CCC'GGG
Cuts at: 1278 1278
Size: 4256
SmlI C'TyrA_G
Cuts at: 287 1246 1411 2326 2818 3080 287
Size: 959 165 915 492 262 1463
Fragments arranged by size:
1463 959 915 492 262 165
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4256
SphI G_CATG'C
Cuts at: 1309 1809 1309
Size: 500 3756
StyI C'CwwG_G
Cuts at: 107 620 1390 1744 107
Size: 513 770 354 2619
Fragments arranged by size:
2619 770 513 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1839 1972 2431 2640 1839
Size: 133 459 209 3455
Fragments arranged by size:
3455 459 209 133
TatI w'GTAC_w
Cuts at: 3771 3771
Size: 4256
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 461 493 579 611 722 754 865 897 3773
Size: 32 86 32 111 32 111 32 2876
Cuts at: 3773 3805 461
Size: 32 912
Fragments arranged by size:
2876 912 111 111 86 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2053 2053
Size: 4256
XbaI T'CTAG_A
Cuts at: 1287 1287
Size: 4256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1081 1102 1751 1081
Size: 21 649 3586
Fragments arranged by size:
3586 649 21
XmaI C'CCGG_G
Cuts at: 1276 1276
Size: 4256
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BbsI BfrBI BmtI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspXI PsrI
SacII SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI