Restriction map for
pF1KB8355
(Circular) (Six-base) MAPSORT of: pF1KB8355.seq Check: 6164 from: 1 to: 5066
pF1KB8355 5066 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1856 1856
Size: 5066
AccI GT'mk_AC
Cuts at: 449 2104 449
Size: 1655 3411
Acc65I G'GTAC_C
Cuts at: 2082 2082
Size: 5066
AclI AA'CG_TT
Cuts at: 4906 4906
Size: 5066
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1890 2891 3323 4070 4338 1890
Size: 1001 432 747 268 2618
Fragments arranged by size:
2618 1001 747 432 268
AflIII A'CryG_T
Cuts at: 2418 3522 4499 2418
Size: 1104 977 2985
Fragments arranged by size:
2985 1104 977
AgeI A'CCGG_T
Cuts at: 3454 3454
Size: 5066
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 149 149
Size: 5066
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1363 1395 1363
Size: 32 5034
AlwNI CAG_nnn'CTG
Cuts at: 2371 3938 2371
Size: 1567 3499
ApaI G_GGCC'C
Cuts at: 1179 1599 1179
Size: 420 4646
ApaLI G'TGCA_C
Cuts at: 3836 3836
Size: 5066
ApoI r'AATT_y
Cuts at: 2070 4419 4675 2070
Size: 2349 256 2461
Fragments arranged by size:
2461 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5066
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5066
AvaI C'yCGr_G
Cuts at: 1346 1611 2086 1346
Size: 265 475 4326
Fragments arranged by size:
4326 475 265
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1607 1640 1893 1926 1607
Size: 33 253 33 4747
Fragments arranged by size:
4747 253 33 33
BamHI G'GATC_C
Cuts at: 2091 2091
Size: 5066
BanI G'GyrC_C
Cuts at: 493 592 1098 1338 1638 2082 2496 2744 2779
Size: 99 506 240 300 444 414 248 35
Cuts at: 2779 493
Size: 2780
Fragments arranged by size:
2780 506 444 414 300 248 240 99
35
BanII G_rGCy'C
Cuts at: 204 587 1126 1179 1253 1325 1592 1599 1772
Size: 383 539 53 74 72 267 7 173
Cuts at: 1772 1844 1949 2080 3110 204
Size: 72 105 131 1030 2160
Fragments arranged by size:
2160 1030 539 383 267 173 131 105
74 72 72 53 7
BbeI G_GCGC'C
Cuts at: 1102 1342 2500 2748 1102
Size: 240 1158 248 3420
Fragments arranged by size:
3420 1158 248 240
BbsI GAAGACnn'nnnn_
Cuts at: 485 613 485
Size: 128 4938
BbvCI CC'TCA_GC
Cuts at: 1803 1803
Size: 5066
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1675 1709 1675
Size: 34 5032
BciVI GTATCCnnnnn_n'
Cuts at: 2964 3725 5066 2964
Size: 761 1341 2964
Fragments arranged by size:
2964 1341 761
BclI T'GATC_A
Cuts at: 2586 2586
Size: 5066
BglI GCCn_nnn'nGGC
Cuts at: 1297 1297
Size: 5066
BglII A'GATC_T
Cuts at: 2581 4618 2581
Size: 2037 3029
BlpI GC'TnA_GC
Cuts at: 1160 2178 1160
Size: 1018 4048
Bme1580I G_kGCm'C
Cuts at: 595 1179 1599 1643 2691 2784 3840 595
Size: 584 420 44 1048 93 1056 1821
Fragments arranged by size:
1821 1056 1048 584 420 93 44
BmgBI CAC'GTC
Cuts at: 453 453
Size: 5066
BmrI ACTGGGnnnn_n'
Cuts at: 375 2017 2278 2359 2694 4838 375
Size: 1642 261 81 335 2144 603
Fragments arranged by size:
2144 1642 603 335 261 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 200 1209 3433 200
Size: 1009 2224 1833
Fragments arranged by size:
2224 1833 1009
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 519 1119 1564 1840 1973 180
Size: 339 600 445 276 133 3273
Fragments arranged by size:
3273 600 445 339 276 133
Bpu10I CC'TnA_GC
Cuts at: 422 724 1724 1803 422
Size: 302 1000 79 3685
Fragments arranged by size:
3685 1000 302 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 1831 2242 3121 3613 3911 220
Size: 1611 411 879 492 298 1375
Fragments arranged by size:
1611 1375 879 492 411 298
BsaI GGTCTCn'nnnn_
Cuts at: 904 1234 1270 1858 4770 904
Size: 330 36 588 2912 1200
Fragments arranged by size:
2912 1200 588 330 36
BsaAI yAC'GTr
Cuts at: 354 417 2419 354
Size: 63 2002 3001
Fragments arranged by size:
3001 2002 63
BsaBI GATnn'nnATC
Cuts at: 6 1999 2580 4617 6
Size: 1993 581 2037 455
Fragments arranged by size:
2037 1993 581 455
BsaHI Gr'CG_yC
Cuts at: 1099 1339 1439 2497 2745 3447 4943 1099
Size: 240 100 1058 248 702 1496 1222
Fragments arranged by size:
1496 1222 1058 702 248 240 100
BsaWI w'CCGG_w
Cuts at: 1088 1662 2255 2776 3454 3728 3875 4286 1088
Size: 574 593 521 678 274 147 411 1868
Fragments arranged by size:
1868 678 593 574 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1912 1942 1912
Size: 30 5036
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 706 709 784 826 916 958 1185 1288 1333
Size: 3 75 42 90 42 227 103 45
Cuts at: 1333 1828 1936 1939 2050 2053 706
Size: 495 108 3 111 3 3719
Fragments arranged by size:
3719 495 227 111 108 103 90 75
45 42 42 3 3 3
BseYI C'CCAG_C
Cuts at: 112 965 1252 2009 2491 3249 3826 4322 112
Size: 853 287 757 482 758 577 496 856
Fragments arranged by size:
856 853 758 757 577 496 482 287
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 532
Size: 5066
BsiEI CG_ry'CG
Cuts at: 104 2608 2654 3862 104
Size: 2504 46 1208 1308
Fragments arranged by size:
2504 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 359 818 1949 2080 2858 3048 3840 359
Size: 459 1131 131 778 190 792 1585
Fragments arranged by size:
1585 1131 792 778 459 190 131
Bsp1286I G_dGCh'C
Cuts at: 204 359 587 595 818 1126 1179 1253 1325
Size: 155 228 8 223 308 53 74 72
Cuts at: 1325 1592 1599 1643 1772 1844 1949 2080 2691
Size: 267 7 44 129 72 105 131 611
Cuts at: 2691 2784 2858 3048 3110 3840 204
Size: 93 74 190 62 730 1430
Fragments arranged by size:
1430 730 611 308 267 228 223 190
155 131 129 105 93 74 74 72
72 62 53 44 8 7
BspEI T'CCGG_A
Cuts at: 1662 4286 1662
Size: 2624 2442
BspHI T'CATG_A
Cuts at: 4242 4242
Size: 5066
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1856 2116 2330 2632 3013 1856
Size: 260 214 302 381 3909
Fragments arranged by size:
3909 381 302 260 214
BsrBI CCG'CTC
Cuts at: 50 699 741 756 780 795 1173 3358 3412
Size: 649 42 15 24 15 378 2185 54
Cuts at: 3412 4896 5061 50
Size: 1484 165 55
Fragments arranged by size:
2185 1484 649 378 165 55 54 42
24 15 15
BsrDI GCAATG_nn'
Cuts at: 2978 2978
Size: 5066
BsrFI r'CCGG_y
Cuts at: 1306 3064 3245 3454 1306
Size: 1758 181 209 2918
Fragments arranged by size:
2918 1758 209 181
BsrGI T'GTAC_A
Cuts at: 206 206
Size: 5066
BssSI C'ACGA_G
Cuts at: 187 3337 3695 187
Size: 3150 358 1558
Fragments arranged by size:
3150 1558 358
BstAPI GCAn_nnn'nTGC
Cuts at: 555 2443 555
Size: 1888 3178
BstBI TT'CG_AA
Cuts at: 3427 3427
Size: 5066
BstEII G'GTnAC_C
Cuts at: 886 1188 1623 886
Size: 302 435 4329
Fragments arranged by size:
4329 435 302
BstXI CCAn_nnnn'nTGG
Cuts at: 417 474 417
Size: 57 5009
BstYI r'GATC_y
Cuts at: 1 2091 2558 2581 2916 3162 4174 4260 4272
Size: 2090 467 23 335 246 1012 86 12
Cuts at: 4272 4618 1
Size: 346 449
Fragments arranged by size:
2090 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1295 1328 1295
Size: 33 5033
BtgI C'CryG_G
Cuts at: 107 121 587 1313 1418 1442 107
Size: 14 466 726 105 24 3731
Fragments arranged by size:
3731 726 466 105 24 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3016 3197 3360 3016
Size: 181 163 4722
Fragments arranged by size:
4722 181 163
BtsI GCAGTG_nn'
Cuts at: 324 1210 1481 1487 1669 2309 324
Size: 886 271 6 182 640 3081
Fragments arranged by size:
3081 886 640 271 182 6
DraI TTT'AAA
Cuts at: 2065 2065
Size: 5066
DrdI GACnn_nn'nnGTC
Cuts at: 2772 3630 2772
Size: 858 4208
EaeI y'GGCC_r
Cuts at: 143 2651 2825 3216 3243 4588 143
Size: 2508 174 391 27 1345 621
Fragments arranged by size:
2508 1345 621 391 174 27
EagI C'GGCC_G
Cuts at: 2651 2651
Size: 5066
EarI CTCTTCn'nnn_
Cuts at: 671 842 902 1937 2027 3089 3299 671
Size: 171 60 1035 90 1062 210 2438
Fragments arranged by size:
2438 1062 1035 210 171 90 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1952 3274 3584 3730 4876 1952
Size: 1322 310 146 1146 2142
Fragments arranged by size:
2142 1322 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1947 2078 1947
Size: 131 4935
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 519 1119 1564 1840 1890 1973 2891 3323
Size: 339 600 445 276 50 83 918 432
Cuts at: 3323 4070 4338 180
Size: 747 268 908
Fragments arranged by size:
918 908 747 600 445 432 339 276
268 83 50
EcoNI CCTnn'n_nnAGG
Cuts at: 1183 1633 1183
Size: 450 4616
EcoO109I rG'GnC_Cy
Cuts at: 708 1176 1568 1595 1596 1883 2205 708
Size: 468 392 27 1 287 322 3569
Fragments arranged by size:
3569 468 392 322 287 27 1
EcoRI G'AATT_C
Cuts at: 2070 2070
Size: 5066
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3085 3085
Size: 5066
FseI GG_CCGG'CC
Cuts at: 1310 1310
Size: 5066
FspI TGC'GCA
Cuts at: 2847 2847
Size: 5066
HaeII r_GCGC'y
Cuts at: 786 1102 1342 2500 2748 3770 4749 786
Size: 316 240 1158 248 1022 979 1103
Fragments arranged by size:
1158 1103 1022 979 316 248 240
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 170 202 1030 1062 1779 1811 2908 2938 2940
Size: 32 828 32 717 32 1097 30 2
Cuts at: 2940 2970 170
Size: 30 2266
Fragments arranged by size:
2266 1097 828 717 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 450 2105 450
Size: 1655 3411
HindIII A'AGCT_T
Cuts at: 238 238
Size: 5066
KasI G'GCGC_C
Cuts at: 1098 1338 2496 2744 1098
Size: 240 1158 248 3420
Fragments arranged by size:
3420 1158 248 240
KpnI G_GTAC'C
Cuts at: 2086 2086
Size: 5066
MluI A'CGCG_T
Cuts at: 4499 4499
Size: 5066
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 914 3737 3921 914
Size: 2823 184 2059
Fragments arranged by size:
2823 2059 184
MscI TGG'CCA
Cuts at: 145 2827 145
Size: 2682 2384
MslI CAynn'nnrTG
Cuts at: 896 3055 3182 896
Size: 2159 127 2780
Fragments arranged by size:
2780 2159 127
MspA1I CmG'CkG
Cuts at: 507 619 969 1034 1435 1462 1525 2177 2491
Size: 112 350 65 401 27 63 652 314
Cuts at: 2491 2851 3864 4109 507
Size: 360 1013 245 1464
Fragments arranged by size:
1464 1013 652 401 360 350 314 245
112 65 63 27
NaeI GCC'GGC
Cuts at: 1308 3247 1308
Size: 1939 3127
NarI GG'CG_CC
Cuts at: 1099 1339 2497 2745 1099
Size: 240 1158 248 3420
Fragments arranged by size:
3420 1158 248 240
NcoI C'CATG_G
Cuts at: 107 121 1313 107
Size: 14 1192 3860
Fragments arranged by size:
3860 1192 14
NdeI CA'TA_TG
Cuts at: 213 213
Size: 5066
NgoMIV G'CCGG_C
Cuts at: 1306 3245 1306
Size: 1939 3127
NspI r_CATG'y
Cuts at: 2009 2119 2619 3526 2009
Size: 110 500 907 3549
Fragments arranged by size:
3549 907 500 110
PasI CC'CwG_GG
Cuts at: 1592 1592
Size: 5066
PciI A'CATG_T
Cuts at: 3522 3522
Size: 5066
PflMI CCAn_nnn'nTGG
Cuts at: 127 127
Size: 5066
PmeI GTTT'AAAC
Cuts at: 2065 2065
Size: 5066
PmlI CAC'GTG
Cuts at: 417 417
Size: 5066
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4205 4237 4205
Size: 32 5034
PpuMI rG'GwC_Cy
Cuts at: 1883 1883
Size: 5066
PspOMI G'GGCC_C
Cuts at: 1175 1595 1175
Size: 420 4646
PstI C_TGCA'G
Cuts at: 138 199 319 1368 1395 2113 2798 138
Size: 61 120 1049 27 718 685 2406
Fragments arranged by size:
2406 1049 718 685 120 61 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5066
PvuII CAG'CTG
Cuts at: 619 1034 1435 1462 2491 2851 619
Size: 415 401 27 1029 360 2834
Fragments arranged by size:
2834 1029 415 401 360 27
RsrII CG'GwC_CG
Cuts at: 3261 3261
Size: 5066
SacI G_AGCT'C
Cuts at: 1949 2080 1949
Size: 131 4935
SalI G'TCGA_C
Cuts at: 448 2103 448
Size: 1655 3411
SanDI GG'GwC_CC
Cuts at: 1883 1883
Size: 5066
SapI GCTCTTCn'nnn_
Cuts at: 671 1937 3089 3299 671
Size: 1266 1152 210 2438
Fragments arranged by size:
2438 1266 1152 210
SbfI CC_TGCA'GG
Cuts at: 199 2113 199
Size: 1914 3152
ScaI AGT'ACT
Cuts at: 4583 4583
Size: 5066
SfcI C'TryA_G
Cuts at: 33 134 195 315 1364 1391 2109 2794 3787
Size: 101 61 120 1049 27 718 685 993
Cuts at: 3787 3978 33
Size: 191 1121
Fragments arranged by size:
1121 1049 993 718 685 191 120 101
61 27
SfoI GGC'GCC
Cuts at: 1100 1340 2498 2746 1100
Size: 240 1158 248 3420
Fragments arranged by size:
3420 1158 248 240
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5066
SmaI CCC'GGG
Cuts at: 1348 2088 1348
Size: 740 4326
SmlI C'TyrA_G
Cuts at: 235 1810 2221 3136 3628 3890 235
Size: 1575 411 915 492 262 1411
Fragments arranged by size:
1575 1411 915 492 411 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5066
SphI G_CATG'C
Cuts at: 2119 2619 2119
Size: 500 4566
StuI AGG'CCT
Cuts at: 157 1300 157
Size: 1143 3923
StyI C'CwwG_G
Cuts at: 107 121 982 1313 1955 2200 2554 107
Size: 14 861 331 642 245 354 2619
Fragments arranged by size:
2619 861 642 354 331 245 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 357 1885 2649 2782 3241 3450 357
Size: 1528 764 133 459 209 1973
Fragments arranged by size:
1973 1528 764 459 209 133
TatI w'GTAC_w
Cuts at: 206 4581 206
Size: 4375 691
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4583 4615 4583
Size: 32 5034
Tth111I GACn'n_nGTC
Cuts at: 2863 2863
Size: 5066
XbaI T'CTAG_A
Cuts at: 2097 2097
Size: 5066
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2561 2561
Size: 5066
XmaI C'CCGG_G
Cuts at: 1346 2086 1346
Size: 740 4326
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AscI AvrII BfrBI
BmtI BsiWI BsmI BsmBI BssHII BstZ17I ClaI CspCI
DraIII EcoRV FspAI HpaI MfeI NheI NotI NruI
NsiI PacI PfoI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI