Restriction map for
pF1KB8354
(Circular) (Six-base) MAPSORT of: pF1KB8354.seq Check: 5181 from: 1 to: 4484
pF1KB8354 4484 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 938 1522 938
Size: 584 3900
Acc65I G'GTAC_C
Cuts at: 1500 1500
Size: 4484
AclI AA'CG_TT
Cuts at: 4324 4324
Size: 4484
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1111 1391 2309 2741 3488 3756 1111
Size: 280 918 432 747 268 1839
Fragments arranged by size:
1839 918 747 432 280 268
AflII C'TTAA_G
Cuts at: 847 847
Size: 4484
AflIII A'CryG_T
Cuts at: 1340 1836 2940 3917 1340
Size: 496 1104 977 1907
Fragments arranged by size:
1907 1104 977 496
AgeI A'CCGG_T
Cuts at: 2872 2872
Size: 4484
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 976 976
Size: 4484
AlwNI CAG_nnn'CTG
Cuts at: 157 1789 3356 157
Size: 1632 1567 1285
Fragments arranged by size:
1632 1567 1285
ApaLI G'TGCA_C
Cuts at: 3254 3254
Size: 4484
ApoI r'AATT_y
Cuts at: 470 802 1488 3837 4093 470
Size: 332 686 2349 256 861
Fragments arranged by size:
2349 861 686 332 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4484
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4484
AvaI C'yCGr_G
Cuts at: 1504 1504
Size: 4484
BamHI G'GATC_C
Cuts at: 1509 1509
Size: 4484
BanI G'GyrC_C
Cuts at: 124 1500 1914 2162 2197 124
Size: 1376 414 248 35 2411
Fragments arranged by size:
2411 1376 414 248 35
BanII G_rGCy'C
Cuts at: 509 1498 2528 509
Size: 989 1030 2465
Fragments arranged by size:
2465 1030 989
BbeI G_GCGC'C
Cuts at: 1918 2166 1918
Size: 248 4236
BbsI GAAGACnn'nnnn_
Cuts at: 695 1408 695
Size: 713 3771
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 431 465 431
Size: 34 4450
BciVI GTATCCnnnnn_n'
Cuts at: 879 2382 3143 4484 879
Size: 1503 761 1341 879
Fragments arranged by size:
1503 1341 879 761
BclI T'GATC_A
Cuts at: 2004 2004
Size: 4484
BfrBI ATG'CAT
Cuts at: 442 442
Size: 4484
BglII A'GATC_T
Cuts at: 1277 1999 4036 1277
Size: 722 2037 1725
Fragments arranged by size:
2037 1725 722
BlpI GC'TnA_GC
Cuts at: 164 824 1596 164
Size: 660 772 3052
Fragments arranged by size:
3052 772 660
Bme1580I G_kGCm'C
Cuts at: 2109 2202 3258 2109
Size: 93 1056 3335
Fragments arranged by size:
3335 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1696 1777 2112 4256 1696
Size: 81 335 2144 1924
Fragments arranged by size:
2144 1924 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2851 2851
Size: 4484
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 745 745
Size: 4484
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1660 2539 3031 3329 1660
Size: 879 492 298 2815
Fragments arranged by size:
2815 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4188 4188
Size: 4484
BsaAI yAC'GTr
Cuts at: 1476 1837 1476
Size: 361 4123
BsaBI GATnn'nnATC
Cuts at: 6 1998 4035 6
Size: 1992 2037 455
Fragments arranged by size:
2037 1992 455
BsaHI Gr'CG_yC
Cuts at: 1915 2163 2865 4361 1915
Size: 248 702 1496 2038
Fragments arranged by size:
2038 1496 702 248
BsaWI w'CCGG_w
Cuts at: 554 1673 2194 2872 3146 3293 3704 554
Size: 1119 521 678 274 147 411 1334
Fragments arranged by size:
1334 1119 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 248 278 593 623 248
Size: 30 315 30 4109
Fragments arranged by size:
4109 315 30 30
BseYI C'CCAG_C
Cuts at: 1187 1909 2667 3244 3740 1187
Size: 722 758 577 496 1931
Fragments arranged by size:
1931 758 722 577 496
BsiEI CG_ry'CG
Cuts at: 104 2026 2072 3280 104
Size: 1922 46 1208 1308
Fragments arranged by size:
1922 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 509 1310 1498 2276 2466 3258 509
Size: 801 188 778 190 792 1735
Fragments arranged by size:
1735 801 792 778 190 188
BsmI GAATG_Cn'
Cuts at: 486 667 1126 486
Size: 181 459 3844
Fragments arranged by size:
3844 459 181
Bsp1286I G_dGCh'C
Cuts at: 509 1310 1498 2109 2202 2276 2466 2528 3258
Size: 801 188 611 93 74 190 62 730
Cuts at: 3258 509
Size: 1735
Fragments arranged by size:
1735 801 730 611 190 188 93 74
62
BspEI T'CCGG_A
Cuts at: 554 3704 554
Size: 3150 1334
BspHI T'CATG_A
Cuts at: 318 1196 3660 318
Size: 878 2464 1142
Fragments arranged by size:
2464 1142 878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1534 1748 2050 2431 1534
Size: 214 302 381 3587
Fragments arranged by size:
3587 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1317 2776 2830 4314 4479 50
Size: 1267 1459 54 1484 165 55
Fragments arranged by size:
1484 1459 1267 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1397 1454 2396 1397
Size: 57 942 3485
Fragments arranged by size:
3485 942 57
BsrFI r'CCGG_y
Cuts at: 2482 2663 2872 2482
Size: 181 209 4094
Fragments arranged by size:
4094 209 181
BssSI C'ACGA_G
Cuts at: 2755 3113 2755
Size: 358 4126
BstAPI GCAn_nnn'nTGC
Cuts at: 1861 1861
Size: 4484
BstBI TT'CG_AA
Cuts at: 857 2845 857
Size: 1988 2496
BstYI r'GATC_y
Cuts at: 1 1277 1509 1976 1999 2334 2580 3592 3678
Size: 1276 232 467 23 335 246 1012 86
Cuts at: 3678 3690 4036 1
Size: 12 346 449
Fragments arranged by size:
1276 1012 467 449 346 335 246 232
86 23 12
BstZ17I GTA'TAC
Cuts at: 939 939
Size: 4484
Bsu36I CC'TnA_GG
Cuts at: 1466 1466
Size: 4484
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2434 2615 2778 2434
Size: 181 163 4140
Fragments arranged by size:
4140 181 163
BtsI GCAGTG_nn'
Cuts at: 1727 1727
Size: 4484
DraI TTT'AAA
Cuts at: 1483 1483
Size: 4484
DrdI GACnn_nn'nnGTC
Cuts at: 2190 3048 2190
Size: 858 3626
EaeI y'GGCC_r
Cuts at: 1076 2069 2243 2634 2661 4006 1076
Size: 993 174 391 27 1345 1554
Fragments arranged by size:
1554 1345 993 391 174 27
EagI C'GGCC_G
Cuts at: 2069 2069
Size: 4484
EarI CTCTTCn'nnn_
Cuts at: 1370 1451 2507 2717 1370
Size: 81 1056 210 3137
Fragments arranged by size:
3137 1056 210 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2692 3002 3148 4294 2692
Size: 310 146 1146 2882
Fragments arranged by size:
2882 1146 310 146
EcoICRI GAG'CTC
Cuts at: 507 1496 507
Size: 989 3495
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 745 1111 1391 2309 2741 3488 3756 745
Size: 366 280 918 432 747 268 1473
Fragments arranged by size:
1473 918 747 432 366 280 268
EcoO109I rG'GnC_Cy
Cuts at: 1134 1623 1134
Size: 489 3995
EcoRI G'AATT_C
Cuts at: 1488 1488
Size: 4484
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 862 1242 2503 862
Size: 380 1261 2843
Fragments arranged by size:
2843 1261 380
FspI TGC'GCA
Cuts at: 2265 2265
Size: 4484
HaeII r_GCGC'y
Cuts at: 1918 2166 3188 4167 1918
Size: 248 1022 979 2235
Fragments arranged by size:
2235 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 169 201 536 568 1195 1227 1262 1294 2326
Size: 32 335 32 627 32 35 32 1032
Cuts at: 2326 2356 2358 2388 169
Size: 30 2 30 2265
Fragments arranged by size:
2265 1032 627 335 35 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1523 1523
Size: 4484
HindIII A'AGCT_T
Cuts at: 1037 1093 1037
Size: 56 4428
KasI G'GCGC_C
Cuts at: 1914 2162 1914
Size: 248 4236
KpnI G_GTAC'C
Cuts at: 1504 1504
Size: 4484
MfeI C'AATT_G
Cuts at: 704 704
Size: 4484
MluI A'CGCG_T
Cuts at: 3917 3917
Size: 4484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3155 3339 3155
Size: 184 4300
MscI TGG'CCA
Cuts at: 1078 2245 1078
Size: 1167 3317
MslI CAynn'nnrTG
Cuts at: 385 2473 2600 385
Size: 2088 127 2269
Fragments arranged by size:
2269 2088 127
MspA1I CmG'CkG
Cuts at: 632 738 1191 1595 1909 2269 3282 3527 632
Size: 106 453 404 314 360 1013 245 1589
Fragments arranged by size:
1589 1013 453 404 360 314 245 106
NaeI GCC'GGC
Cuts at: 2665 2665
Size: 4484
NarI GG'CG_CC
Cuts at: 1915 2163 1915
Size: 248 4236
NdeI CA'TA_TG
Cuts at: 444 444
Size: 4484
NgoMIV G'CCGG_C
Cuts at: 2663 2663
Size: 4484
NsiI A_TGCA'T
Cuts at: 444 444
Size: 4484
NspI r_CATG'y
Cuts at: 963 1344 1537 2037 2944 963
Size: 381 193 500 907 2503
Fragments arranged by size:
2503 907 500 381 193
PciI A'CATG_T
Cuts at: 1340 2940 1340
Size: 1600 2884
PflMI CCAn_nnn'nTGG
Cuts at: 775 775
Size: 4484
PmeI GTTT'AAAC
Cuts at: 1483 1483
Size: 4484
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3623 3655 3623
Size: 32 4452
PstI C_TGCA'G
Cuts at: 658 1277 1531 2216 658
Size: 619 254 685 2926
Fragments arranged by size:
2926 685 619 254
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4484
PvuII CAG'CTG
Cuts at: 1191 1909 2269 1191
Size: 718 360 3406
Fragments arranged by size:
3406 718 360
RsrII CG'GwC_CG
Cuts at: 2679 2679
Size: 4484
SacI G_AGCT'C
Cuts at: 509 1498 509
Size: 989 3495
SalI G'TCGA_C
Cuts at: 1521 1521
Size: 4484
SapI GCTCTTCn'nnn_
Cuts at: 2507 2717 2507
Size: 210 4274
SbfI CC_TGCA'GG
Cuts at: 1531 1531
Size: 4484
ScaI AGT'ACT
Cuts at: 4001 4001
Size: 4484
SfcI C'TryA_G
Cuts at: 33 654 1273 1527 2212 3205 3396 33
Size: 621 619 254 685 993 191 1121
Fragments arranged by size:
1121 993 685 621 619 254 191
SfoI GGC'GCC
Cuts at: 1916 2164 1916
Size: 248 4236
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4484
SmaI CCC'GGG
Cuts at: 1506 1506
Size: 4484
SmlI C'TyrA_G
Cuts at: 847 1639 2554 3046 3308 847
Size: 792 915 492 262 2023
Fragments arranged by size:
2023 915 792 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4484
SphI G_CATG'C
Cuts at: 1537 2037 1537
Size: 500 3984
StyI C'CwwG_G
Cuts at: 1137 1217 1301 1370 1618 1972 1137
Size: 80 84 69 248 354 3649
Fragments arranged by size:
3649 354 248 84 80 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2067 2200 2659 2868 2067
Size: 133 459 209 3683
Fragments arranged by size:
3683 459 209 133
TatI w'GTAC_w
Cuts at: 3999 3999
Size: 4484
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4001 4033 4001
Size: 32 4452
Tth111I GACn'n_nGTC
Cuts at: 2281 2281
Size: 4484
XbaI T'CTAG_A
Cuts at: 1515 1515
Size: 4484
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 897 1389 1979 897
Size: 492 590 3402
Fragments arranged by size:
3402 590 492
XmaI C'CCGG_G
Cuts at: 1504 1504
Size: 4484
XmnI GAAnn'nnTTC
Cuts at: 1049 1049
Size: 4484
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NsiI NspI PciI PflMI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BglI BmgBI BmtI Bpu10I BseRI
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
NcoI NheI NotI NruI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI