Restriction map for
pF1KB8353
(Circular) (Six-base) MAPSORT of: pF1KB8353.seq Check: 1348 from: 1 to: 4154
pF1KB8353 4154 bp
With 182 enzymes: SgfI *
July 2, 2008 13:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 863 863
Size: 4154
AccI GT'mk_AC
Cuts at: 716 872 1192 716
Size: 156 320 3678
Fragments arranged by size:
3678 320 156
Acc65I G'GTAC_C
Cuts at: 1016 1170 1016
Size: 154 4000
AclI AA'CG_TT
Cuts at: 3994 3994
Size: 4154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1979 2411 3158 3426 1979
Size: 432 747 268 2707
Fragments arranged by size:
2707 747 432 268
AflIII A'CryG_T
Cuts at: 1506 2610 3587 1506
Size: 1104 977 2073
Fragments arranged by size:
2073 1104 977
AgeI A'CCGG_T
Cuts at: 2542 2542
Size: 4154
AlwNI CAG_nnn'CTG
Cuts at: 1459 3026 1459
Size: 1567 2587
ApaLI G'TGCA_C
Cuts at: 328 2924 328
Size: 2596 1558
ApoI r'AATT_y
Cuts at: 1158 3507 3763 1158
Size: 2349 256 1549
Fragments arranged by size:
2349 1549 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4154
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4154
AvaI C'yCGr_G
Cuts at: 895 1174 895
Size: 279 3875
BamHI G'GATC_C
Cuts at: 1179 1179
Size: 4154
BanI G'GyrC_C
Cuts at: 883 987 1016 1170 1584 1832 1867 883
Size: 104 29 154 414 248 35 3170
Fragments arranged by size:
3170 414 248 154 104 35 29
BanII G_rGCy'C
Cuts at: 356 1079 1168 2198 356
Size: 723 89 1030 2312
Fragments arranged by size:
2312 1030 723 89
BbeI G_GCGC'C
Cuts at: 1588 1836 1588
Size: 248 3906
BbvCI CC'TCA_GC
Cuts at: 439 439
Size: 4154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4120
BciVI GTATCCnnnnn_n'
Cuts at: 2052 2813 4154 2052
Size: 761 1341 2052
Fragments arranged by size:
2052 1341 761
BclI T'GATC_A
Cuts at: 242 1674 242
Size: 1432 2722
BglI GCCn_nnn'nGGC
Cuts at: 757 935 757
Size: 178 3976
BglII A'GATC_T
Cuts at: 1669 3706 1669
Size: 2037 2117
BlpI GC'TnA_GC
Cuts at: 1266 1266
Size: 4154
Bme1580I G_kGCm'C
Cuts at: 332 793 992 1148 1779 1872 2928 332
Size: 461 199 156 631 93 1056 1558
Fragments arranged by size:
1558 1056 631 461 199 156 93
BmgBI CAC'GTC
Cuts at: 864 864
Size: 4154
BmrI ACTGGGnnnn_n'
Cuts at: 1366 1447 1782 3926 1366
Size: 81 335 2144 1594
Fragments arranged by size:
2144 1594 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 639 2521 639
Size: 1882 2272
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 525 609 802 829 220
Size: 305 84 193 27 3545
Fragments arranged by size:
3545 305 193 84 27
Bpu10I CC'TnA_GC
Cuts at: 439 439
Size: 4154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 1330 2209 2701 2999 340
Size: 990 879 492 298 1495
Fragments arranged by size:
1495 990 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3858 3858
Size: 4154
BsaAI yAC'GTr
Cuts at: 1507 1507
Size: 4154
BsaBI GATnn'nnATC
Cuts at: 6 653 1668 3705 6
Size: 647 1015 2037 455
Fragments arranged by size:
2037 1015 647 455
BsaHI Gr'CG_yC
Cuts at: 1585 1833 2535 4031 1585
Size: 248 702 1496 1708
Fragments arranged by size:
1708 1496 702 248
BsaWI w'CCGG_w
Cuts at: 965 1343 1864 2542 2816 2963 3374 965
Size: 378 521 678 274 147 411 1745
Fragments arranged by size:
1745 678 521 411 378 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 523 553 769 799 523
Size: 30 216 30 3878
Fragments arranged by size:
3878 216 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 325 325
Size: 4154
BseYI C'CCAG_C
Cuts at: 122 503 930 1096 1579 2337 2914 3410 122
Size: 381 427 166 483 758 577 496 866
Fragments arranged by size:
866 758 577 496 483 427 381 166
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 445
Size: 4154
BsiEI CG_ry'CG
Cuts at: 104 549 1696 1742 2950 104
Size: 445 1147 46 1208 1308
Fragments arranged by size:
1308 1208 1147 445 46
BsiHKAI G_wGCw'C
Cuts at: 332 356 854 1168 1946 2136 2928 332
Size: 24 498 314 778 190 792 1558
Fragments arranged by size:
1558 792 778 498 314 190 24
BsmBI CGTCTCn'nnnn_
Cuts at: 1107 1107
Size: 4154
Bsp1286I G_dGCh'C
Cuts at: 332 356 793 854 992 1079 1148 1168 1779
Size: 24 437 61 138 87 69 20 611
Cuts at: 1779 1872 1946 2136 2198 2928 332
Size: 93 74 190 62 730 1558
Fragments arranged by size:
1558 730 611 437 190 138 93 87
74 69 62 61 24 20
BspEI T'CCGG_A
Cuts at: 965 3374 965
Size: 2409 1745
BspHI T'CATG_A
Cuts at: 3330 3330
Size: 4154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 326 863 1204 1418 1720 2101 326
Size: 537 341 214 302 381 2379
Fragments arranged by size:
2379 537 381 341 302 214
BsrBI CCG'CTC
Cuts at: 50 2446 2500 3984 4149 50
Size: 2396 54 1484 165 55
Fragments arranged by size:
2396 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 669 1040 2066 669
Size: 371 1026 2757
Fragments arranged by size:
2757 1026 371
BsrFI r'CCGG_y
Cuts at: 2152 2333 2542 2152
Size: 181 209 3764
Fragments arranged by size:
3764 209 181
BsrGI T'GTAC_A
Cuts at: 701 701
Size: 4154
BssSI C'ACGA_G
Cuts at: 333 2425 2783 333
Size: 2092 358 1704
Fragments arranged by size:
2092 1704 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1531 1531
Size: 4154
BstBI TT'CG_AA
Cuts at: 2515 2515
Size: 4154
BstEII G'GTnAC_C
Cuts at: 1002 1002
Size: 4154
BstXI CCAn_nnnn'nTGG
Cuts at: 516 999 516
Size: 483 3671
BstYI r'GATC_y
Cuts at: 1 1179 1646 1669 2004 2250 3262 3348 3360
Size: 1178 467 23 335 246 1012 86 12
Cuts at: 3360 3706 1
Size: 346 449
Fragments arranged by size:
1178 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 873 873
Size: 4154
Bsu36I CC'TnA_GG
Cuts at: 1008 1008
Size: 4154
BtgI C'CryG_G
Cuts at: 549 975 549
Size: 426 3728
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2104 2285 2448 2104
Size: 181 163 3810
Fragments arranged by size:
3810 181 163
BtsI GCAGTG_nn'
Cuts at: 534 1148 1397 534
Size: 614 249 3291
Fragments arranged by size:
3291 614 249
DraI TTT'AAA
Cuts at: 1153 1153
Size: 4154
DrdI GACnn_nn'nnGTC
Cuts at: 1860 2718 1860
Size: 858 3296
EaeI y'GGCC_r
Cuts at: 154 978 1739 1913 2304 2331 3676 154
Size: 824 761 174 391 27 1345 632
Fragments arranged by size:
1345 824 761 632 391 174 27
EagI C'GGCC_G
Cuts at: 1739 1739
Size: 4154
EarI CTCTTCn'nnn_
Cuts at: 254 2177 2387 254
Size: 1923 210 2021
Fragments arranged by size:
2021 1923 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 783 2362 2672 2818 3964 783
Size: 1579 310 146 1146 973
Fragments arranged by size:
1579 1146 973 310 146
EcoICRI GAG'CTC
Cuts at: 354 1166 354
Size: 812 3342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 525 609 802 829 1979 2411 3158 3426
Size: 305 84 193 27 1150 432 747 268
Cuts at: 3426 220
Size: 948
Fragments arranged by size:
1150 948 747 432 305 268 193 84
27
EcoNI CCTnn'n_nnAGG
Cuts at: 814 814
Size: 4154
EcoO109I rG'GnC_Cy
Cuts at: 315 443 1293 315
Size: 128 850 3176
Fragments arranged by size:
3176 850 128
EcoRI G'AATT_C
Cuts at: 1158 1158
Size: 4154
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 283 381 2173 283
Size: 98 1792 2264
Fragments arranged by size:
2264 1792 98
FspI TGC'GCA
Cuts at: 1935 1935
Size: 4154
HaeII r_GCGC'y
Cuts at: 342 1588 1836 2858 3837 342
Size: 1246 248 1022 979 659
Fragments arranged by size:
1246 1022 979 659 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 327 359 1996 2026 2028 2058 327
Size: 32 1637 30 2 30 2423
Fragments arranged by size:
2423 1637 32 30 30 2
HincII GTy'rAC
Cuts at: 1193 1193
Size: 4154
HindIII A'AGCT_T
Cuts at: 265 265
Size: 4154
KasI G'GCGC_C
Cuts at: 1584 1832 1584
Size: 248 3906
KpnI G_GTAC'C
Cuts at: 1020 1174 1020
Size: 154 4000
MluI A'CGCG_T
Cuts at: 3587 3587
Size: 4154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2825 3009 2825
Size: 184 3970
MscI TGG'CCA
Cuts at: 156 1915 156
Size: 1759 2395
MslI CAynn'nnrTG
Cuts at: 190 286 514 997 2143 2270 190
Size: 96 228 483 1146 127 2074
Fragments arranged by size:
2074 1146 483 228 127 96
MspA1I CmG'CkG
Cuts at: 126 934 1128 1265 1579 1939 2952 3197 126
Size: 808 194 137 314 360 1013 245 1083
Fragments arranged by size:
1083 1013 808 360 314 245 194 137
NaeI GCC'GGC
Cuts at: 2335 2335
Size: 4154
NarI GG'CG_CC
Cuts at: 1585 1833 1585
Size: 248 3906
NgoMIV G'CCGG_C
Cuts at: 2333 2333
Size: 4154
NspI r_CATG'y
Cuts at: 139 285 1207 1707 2614 139
Size: 146 922 500 907 1679
Fragments arranged by size:
1679 922 907 500 146
PasI CC'CwG_GG
Cuts at: 736 736
Size: 4154
PciI A'CATG_T
Cuts at: 2610 2610
Size: 4154
PfoI T'CCnGG_A
Cuts at: 845 845
Size: 4154
PmeI GTTT'AAAC
Cuts at: 1153 1153
Size: 4154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 946 978 3293 3325 946
Size: 32 2315 32 1775
Fragments arranged by size:
2315 1775 32 32
PpuMI rG'GwC_Cy
Cuts at: 315 315
Size: 4154
PspXI vC'TCGA_Gb
Cuts at: 895 895
Size: 4154
PstI C_TGCA'G
Cuts at: 1143 1201 1886 1143
Size: 58 685 3411
Fragments arranged by size:
3411 685 58
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4154
PvuII CAG'CTG
Cuts at: 126 1579 1939 126
Size: 1453 360 2341
Fragments arranged by size:
2341 1453 360
RsrII CG'GwC_CG
Cuts at: 2349 2349
Size: 4154
SacI G_AGCT'C
Cuts at: 356 1168 356
Size: 812 3342
SalI G'TCGA_C
Cuts at: 1191 1191
Size: 4154
SapI GCTCTTCn'nnn_
Cuts at: 2177 2387 2177
Size: 210 3944
SbfI CC_TGCA'GG
Cuts at: 1201 1201
Size: 4154
ScaI AGT'ACT
Cuts at: 3671 3671
Size: 4154
SfcI C'TryA_G
Cuts at: 33 1139 1197 1882 2875 3066 33
Size: 1106 58 685 993 191 1121
Fragments arranged by size:
1121 1106 993 685 191 58
SfoI GGC'GCC
Cuts at: 1586 1834 1586
Size: 248 3906
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4154
SmaI CCC'GGG
Cuts at: 1176 1176
Size: 4154
SmlI C'TyrA_G
Cuts at: 355 895 1309 2224 2716 2978 355
Size: 540 414 915 492 262 1531
Fragments arranged by size:
1531 915 540 492 414 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4154
SphI G_CATG'C
Cuts at: 285 1207 1707 285
Size: 922 500 2732
Fragments arranged by size:
2732 922 500
StuI AGG'CCT
Cuts at: 304 304
Size: 4154
StyI C'CwwG_G
Cuts at: 909 1288 1642 909
Size: 379 354 3421
Fragments arranged by size:
3421 379 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1110 1737 1870 2329 2538 1110
Size: 627 133 459 209 2726
Fragments arranged by size:
2726 627 459 209 133
TatI w'GTAC_w
Cuts at: 164 701 3669 164
Size: 537 2968 649
Fragments arranged by size:
2968 649 537
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 523 555 769 801 960 992 3671 3703 523
Size: 32 214 32 159 32 2679 32 974
Fragments arranged by size:
2679 974 214 159 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1951 1951
Size: 4154
XbaI T'CTAG_A
Cuts at: 1185 1185
Size: 4154
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 149 661 1649 149
Size: 512 988 2654
Fragments arranged by size:
2654 988 512
XhoI C'TCGA_G
Cuts at: 895 895
Size: 4154
XmaI C'CCGG_G
Cuts at: 1174 1174
Size: 4154
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PfoI PmeI PpiI PpuMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BfrBI BmtI BsiWI BsmI
BssHII ClaI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PflMI PmlI PshAI PsiI PspOMI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XmnI
ZraI