Restriction map for
pF1KB8352
(Circular) (Six-base) MAPSORT of: pF1KB8352.seq Check: 1260 from: 1 to: 5192
pF1KB8352 5192 bp
With 182 enzymes: SgfI *
July 2, 2008 13:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 461 461
Size: 5192
AccI GT'mk_AC
Cuts at: 2230 2230
Size: 5192
Acc65I G'GTAC_C
Cuts at: 2208 2208
Size: 5192
AclI AA'CG_TT
Cuts at: 5032 5032
Size: 5192
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 1392 3017 3449 4196 4464 928
Size: 464 1625 432 747 268 1656
Fragments arranged by size:
1656 1625 747 464 432 268
AfeI AGC'GCT
Cuts at: 367 367
Size: 5192
AflIII A'CryG_T
Cuts at: 1691 2544 3648 4625 1691
Size: 853 1104 977 2258
Fragments arranged by size:
2258 1104 977 853
AgeI A'CCGG_T
Cuts at: 3580 3580
Size: 5192
AleI CACnn'nnGTG
Cuts at: 1972 1972
Size: 5192
AlwNI CAG_nnn'CTG
Cuts at: 652 1131 1740 2067 2109 2497 4064 652
Size: 479 609 327 42 388 1567 1780
Fragments arranged by size:
1780 1567 609 479 388 327 42
ApaI G_GGCC'C
Cuts at: 127 2091 2162 127
Size: 1964 71 3157
Fragments arranged by size:
3157 1964 71
ApaLI G'TGCA_C
Cuts at: 436 3962 436
Size: 3526 1666
ApoI r'AATT_y
Cuts at: 2176 2196 4545 4801 2176
Size: 20 2349 256 2567
Fragments arranged by size:
2567 2349 256 20
AseI AT'TA_AT
Cuts at: 20 20
Size: 5192
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5192
AvaI C'yCGr_G
Cuts at: 126 531 803 1154 1360 2212 126
Size: 405 272 351 206 852 3106
Fragments arranged by size:
3106 852 405 351 272 206
AvrII C'CTAG_G
Cuts at: 1824 1824
Size: 5192
BamHI G'GATC_C
Cuts at: 1957 2217 1957
Size: 260 4932
BanI G'GyrC_C
Cuts at: 148 171 233 291 491 520 808 1342 1468
Size: 23 62 58 200 29 288 534 126
Cuts at: 1468 2208 2622 2870 2905 148
Size: 740 414 248 35 2435
Fragments arranged by size:
2435 740 534 414 288 248 200 126
62 58 35 29 23
BanII G_rGCy'C
Cuts at: 127 137 224 287 580 659 742 765 833
Size: 10 87 63 293 79 83 23 68
Cuts at: 833 1713 2091 2162 2206 3236 127
Size: 880 378 71 44 1030 2083
Fragments arranged by size:
2083 1030 880 378 293 87 83 79
71 68 63 44 23 10
BbeI G_GCGC'C
Cuts at: 152 295 2626 2874 152
Size: 143 2331 248 2470
Fragments arranged by size:
2470 2331 248 143
BbsI GAAGACnn'nnnn_
Cuts at: 1465 1465
Size: 5192
BbvCI CC'TCA_GC
Cuts at: 597 1124 1511 597
Size: 527 387 4278
Fragments arranged by size:
4278 527 387
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1916 1950 1991 2025 1916
Size: 34 41 34 5083
Fragments arranged by size:
5083 41 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3090 3851 5192 3090
Size: 761 1341 3090
Fragments arranged by size:
3090 1341 761
BclI T'GATC_A
Cuts at: 2712 2712
Size: 5192
BglI GCCn_nnn'nGGC
Cuts at: 424 1829 424
Size: 1405 3787
BglII A'GATC_T
Cuts at: 1601 2707 4744 1601
Size: 1106 2037 2049
Fragments arranged by size:
2049 2037 1106
BlpI GC'TnA_GC
Cuts at: 2304 2304
Size: 5192
Bme1580I G_kGCm'C
Cuts at: 127 440 811 1345 1767 2091 2162 2817 2910
Size: 313 371 534 422 324 71 655 93
Cuts at: 2910 3966 127
Size: 1056 1353
Fragments arranged by size:
1353 1056 655 534 422 371 324 313
93 71
BmrI ACTGGGnnnn_n'
Cuts at: 2404 2485 2820 4964 2404
Size: 81 335 2144 2632
Fragments arranged by size:
2632 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3559 3559
Size: 5192
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 594 689 946 1093 1541 238
Size: 356 95 257 147 448 3889
Fragments arranged by size:
3889 448 356 257 147 95
Bpu10I CC'TnA_GC
Cuts at: 597 653 1124 1511 597
Size: 56 471 387 4278
Fragments arranged by size:
4278 471 387 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 2368 3247 3739 4037 817
Size: 1551 879 492 298 1972
Fragments arranged by size:
1972 1551 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1426 4896 1426
Size: 3470 1722
BsaAI yAC'GTr
Cuts at: 1692 2545 1692
Size: 853 4339
BsaBI GATnn'nnATC
Cuts at: 6 2032 2706 4743 6
Size: 2026 674 2037 455
Fragments arranged by size:
2037 2026 674 455
BsaHI Gr'CG_yC
Cuts at: 149 212 292 298 305 2623 2871 3573 5069
Size: 63 80 6 7 2318 248 702 1496
Cuts at: 5069 149
Size: 272
Fragments arranged by size:
2318 1496 702 272 248 80 63 7
6
BsaWI w'CCGG_w
Cuts at: 2381 2902 3580 3854 4001 4412 2381
Size: 521 678 274 147 411 3161
Fragments arranged by size:
3161 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 958 988 958
Size: 30 5162
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 841 1378 1519 1528 841
Size: 537 141 9 4505
Fragments arranged by size:
4505 537 141 9
BseYI C'CCAG_C
Cuts at: 152 538 618 1186 1251 1300 1884 1940 2617
Size: 386 80 568 65 49 584 56 677
Cuts at: 2617 3375 3952 4448 152
Size: 758 577 496 896
Fragments arranged by size:
896 758 677 584 577 568 496 386
80 65 56 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 536 1627 536
Size: 1091 4101
BsiEI CG_ry'CG
Cuts at: 104 2734 2780 3988 104
Size: 2630 46 1208 1308
Fragments arranged by size:
2630 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 440 659 742 833 1023 1549 2206 2984 3174
Size: 219 83 91 190 526 657 778 190
Cuts at: 3174 3966 440
Size: 792 1666
Fragments arranged by size:
1666 792 778 657 526 219 190 190
91 83
BsmBI CGTCTCn'nnnn_
Cuts at: 1087 1087
Size: 5192
Bsp1286I G_dGCh'C
Cuts at: 127 137 224 287 440 580 659 742 765
Size: 10 87 63 153 140 79 83 23
Cuts at: 765 811 833 1023 1345 1549 1713 1767 2091
Size: 46 22 190 322 204 164 54 324
Cuts at: 2091 2162 2206 2817 2910 2984 3174 3236 3966
Size: 71 44 611 93 74 190 62 730
Cuts at: 3966 127
Size: 1353
Fragments arranged by size:
1353 730 611 324 322 204 190 190
164 153 140 93 87 83 79 74
71 63 62 54 46 44 23 22
10
BspEI T'CCGG_A
Cuts at: 4412 4412
Size: 5192
BspHI T'CATG_A
Cuts at: 4368 4368
Size: 5192
BspMI ACCTGCnnnn'nnnn_
Cuts at: 461 1364 1549 2242 2456 2758 3139 461
Size: 903 185 693 214 302 381 2514
Fragments arranged by size:
2514 903 693 381 302 214 185
BsrBI CCG'CTC
Cuts at: 50 3484 3538 5022 5187 50
Size: 3434 54 1484 165 55
Fragments arranged by size:
3434 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3104 3104
Size: 5192
BsrFI r'CCGG_y
Cuts at: 1903 3190 3371 3580 1903
Size: 1287 181 209 3515
Fragments arranged by size:
3515 1287 209 181
BssHII G'CGCG_C
Cuts at: 277 277
Size: 5192
BssSI C'ACGA_G
Cuts at: 433 3463 3821 433
Size: 3030 358 1804
Fragments arranged by size:
3030 1804 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2569 2569
Size: 5192
BstBI TT'CG_AA
Cuts at: 3553 3553
Size: 5192
BstXI CCAn_nnnn'nTGG
Cuts at: 244 1974 244
Size: 1730 3462
BstYI r'GATC_y
Cuts at: 1 1601 1957 2217 2684 2707 3042 3288 4300
Size: 1600 356 260 467 23 335 246 1012
Cuts at: 4300 4386 4398 4744 1
Size: 86 12 346 449
Fragments arranged by size:
1600 1012 467 449 356 346 335 260
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1894 1913 1894
Size: 19 5173
BtgI C'CryG_G
Cuts at: 107 1197 1729 1805 1973 107
Size: 1090 532 76 168 3326
Fragments arranged by size:
3326 1090 532 168 76
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1575 3142 3323 3486 1575
Size: 1567 181 163 3281
Fragments arranged by size:
3281 1567 181 163
BtsI GCAGTG_nn'
Cuts at: 524 944 1086 1549 2435 524
Size: 420 142 463 886 3281
Fragments arranged by size:
3281 886 463 420 142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 980 1015 980
Size: 35 5157
DraI TTT'AAA
Cuts at: 2191 2191
Size: 5192
DrdI GACnn_nn'nnGTC
Cuts at: 2898 3756 2898
Size: 858 4334
EaeI y'GGCC_r
Cuts at: 110 383 425 2777 2951 3342 3369 4714 110
Size: 273 42 2352 174 391 27 1345 588
Fragments arranged by size:
2352 1345 588 391 273 174 42 27
EagI C'GGCC_G
Cuts at: 2777 2777
Size: 5192
EarI CTCTTCn'nnn_
Cuts at: 895 1496 3215 3425 895
Size: 601 1719 210 2662
Fragments arranged by size:
2662 1719 601 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3400 3710 3856 5002 3400
Size: 310 146 1146 3590
Fragments arranged by size:
3590 1146 310 146
EcoICRI GAG'CTC
Cuts at: 657 740 831 2204 657
Size: 83 91 1373 3645
Fragments arranged by size:
3645 1373 91 83
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 594 689 928 946 1093 1392 1541 3017
Size: 356 95 239 18 147 299 149 1476
Cuts at: 3017 3449 4196 4464 238
Size: 432 747 268 966
Fragments arranged by size:
1476 966 747 432 356 299 268 239
149 147 95 18
EcoNI CCTnn'n_nnAGG
Cuts at: 754 958 1360 1897 2077 2143 754
Size: 204 402 537 180 66 3803
Fragments arranged by size:
3803 537 402 204 180 66
EcoO109I rG'GnC_Cy
Cuts at: 649 1792 2087 2088 2158 2331 649
Size: 1143 295 1 70 173 3510
Fragments arranged by size:
3510 1143 295 173 70 1
EcoRI G'AATT_C
Cuts at: 2196 2196
Size: 5192
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 695 966 3211 695
Size: 271 2245 2676
Fragments arranged by size:
2676 2245 271
FspI TGC'GCA
Cuts at: 2973 2973
Size: 5192
HaeII r_GCGC'y
Cuts at: 152 295 369 1750 2626 2874 3896 4875 152
Size: 143 74 1381 876 248 1022 979 469
Fragments arranged by size:
1381 1022 979 876 469 248 143 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 958 990 1121 1153 1467 1499 3034 3064 3066
Size: 32 131 32 314 32 1535 30 2
Cuts at: 3066 3096 958
Size: 30 3054
Fragments arranged by size:
3054 1535 314 131 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2231 2231
Size: 5192
KasI G'GCGC_C
Cuts at: 148 291 2622 2870 148
Size: 143 2331 248 2470
Fragments arranged by size:
2470 2331 248 143
KpnI G_GTAC'C
Cuts at: 2212 2212
Size: 5192
MluI A'CGCG_T
Cuts at: 4625 4625
Size: 5192
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1241 3863 4047 1241
Size: 2622 184 2386
Fragments arranged by size:
2622 2386 184
MscI TGG'CCA
Cuts at: 2953 2953
Size: 5192
MslI CAynn'nnrTG
Cuts at: 1594 1972 3181 3308 1594
Size: 378 1209 127 3478
Fragments arranged by size:
3478 1209 378 127
MspA1I CmG'CkG
Cuts at: 459 972 1626 1782 2106 2303 2617 2977 3990
Size: 513 654 156 324 197 314 360 1013
Cuts at: 3990 4235 459
Size: 245 1416
Fragments arranged by size:
1416 1013 654 513 360 324 314 245
197 156
NaeI GCC'GGC
Cuts at: 1905 3373 1905
Size: 1468 3724
NarI GG'CG_CC
Cuts at: 149 292 2623 2871 149
Size: 143 2331 248 2470
Fragments arranged by size:
2470 2331 248 143
NcoI C'CATG_G
Cuts at: 107 1197 1729 107
Size: 1090 532 3570
Fragments arranged by size:
3570 1090 532
NgoMIV G'CCGG_C
Cuts at: 1903 3371 1903
Size: 1468 3724
NspI r_CATG'y
Cuts at: 2245 2745 3652 2245
Size: 500 907 3785
Fragments arranged by size:
3785 907 500
PasI CC'CwG_GG
Cuts at: 179 179
Size: 5192
PciI A'CATG_T
Cuts at: 3648 3648
Size: 5192
PflMI CCAn_nnn'nTGG
Cuts at: 1806 2067 1806
Size: 261 4931
PmeI GTTT'AAAC
Cuts at: 2191 2191
Size: 5192
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4331 4363 4331
Size: 32 5160
PspOMI G'GGCC_C
Cuts at: 123 2087 2158 123
Size: 1964 71 3157
Fragments arranged by size:
3157 1964 71
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1018 1050 1018
Size: 32 5160
PstI C_TGCA'G
Cuts at: 458 758 939 1572 1787 2105 2239 2924 458
Size: 300 181 633 215 318 134 685 2726
Fragments arranged by size:
2726 685 633 318 300 215 181 134
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5192
PvuII CAG'CTG
Cuts at: 459 972 1626 1782 2106 2617 2977 459
Size: 513 654 156 324 511 360 2674
Fragments arranged by size:
2674 654 513 511 360 324 156
RsrII CG'GwC_CG
Cuts at: 3387 3387
Size: 5192
SacI G_AGCT'C
Cuts at: 659 742 833 2206 659
Size: 83 91 1373 3645
Fragments arranged by size:
3645 1373 91 83
SalI G'TCGA_C
Cuts at: 2229 2229
Size: 5192
SapI GCTCTTCn'nnn_
Cuts at: 3215 3425 3215
Size: 210 4982
SbfI CC_TGCA'GG
Cuts at: 758 2239 758
Size: 1481 3711
ScaI AGT'ACT
Cuts at: 4709 4709
Size: 5192
SexAI A'CCwGG_T
Cuts at: 1493 1493
Size: 5192
SfcI C'TryA_G
Cuts at: 33 454 754 935 1568 1783 2101 2235 2920
Size: 421 300 181 633 215 318 134 685
Cuts at: 2920 3913 4104 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 633 421 318 300 215
191 181 134
SfoI GGC'GCC
Cuts at: 150 293 2624 2872 150
Size: 143 2331 248 2470
Fragments arranged by size:
2470 2331 248 143
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5192
SmaI CCC'GGG
Cuts at: 128 805 1156 2214 128
Size: 677 351 1058 3106
Fragments arranged by size:
3106 1058 677 351
SmlI C'TyrA_G
Cuts at: 832 2347 3262 3754 4016 832
Size: 1515 915 492 262 2008
Fragments arranged by size:
2008 1515 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5192
SphI G_CATG'C
Cuts at: 2245 2745 2245
Size: 500 4692
SrfI GCCC'GGGC
Cuts at: 1156 1156
Size: 5192
StuI AGG'CCT
Cuts at: 571 1102 1665 571
Size: 531 563 4098
Fragments arranged by size:
4098 563 531
StyI C'CwwG_G
Cuts at: 107 1197 1436 1729 1824 1989 2326 2680 107
Size: 1090 239 293 95 165 337 354 2619
Fragments arranged by size:
2619 1090 354 337 293 239 165 95
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 236 361 826 845 973 1004 1260 1555 2775
Size: 125 465 19 128 31 256 295 1220
Cuts at: 2775 2908 3367 3576 236
Size: 133 459 209 1852
Fragments arranged by size:
1852 1220 465 459 295 256 209 133
128 125 31 19
TatI w'GTAC_w
Cuts at: 1367 4707 1367
Size: 3340 1852
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4709 4741 4709
Size: 32 5160
Tth111I GACn'n_nGTC
Cuts at: 2989 2989
Size: 5192
XbaI T'CTAG_A
Cuts at: 2223 2223
Size: 5192
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1068 1115 1224 1803 2687 1068
Size: 47 109 579 884 3573
Fragments arranged by size:
3573 884 579 109 47
XmaI C'CCGG_G
Cuts at: 126 803 1154 2212 126
Size: 677 351 1058 3106
Fragments arranged by size:
3106 1058 677 351
XmnI GAAnn'nnTTC
Cuts at: 1030 1030
Size: 5192
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PmeI PpiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI BaeI BfrBI
BmgBI BmtI BsiWI BsmI BsrGI BstEII BstZ17I ClaI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PfoI PmlI PpuMI
PshAI PsiI PspXI SacII SanDI SfiI SgrAI SnaBI
SspI SwaI XhoI ZraI