Restriction map for
pF1KB8347
(Circular) (Six-base) MAPSORT of: pF1KB8347.seq Check: 6200 from: 1 to: 4247
pF1KB8347 4247 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 720 720
Size: 4247
AccI GT'mk_AC
Cuts at: 998 1285 998
Size: 287 3960
Acc65I G'GTAC_C
Cuts at: 1263 1263
Size: 4247
AclI AA'CG_TT
Cuts at: 4087 4087
Size: 4247
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 462 2072 2504 3251 3519 331
Size: 131 1610 432 747 268 1059
Fragments arranged by size:
1610 1059 747 432 268 131
AfeI AGC'GCT
Cuts at: 1111 1111
Size: 4247
AflIII A'CryG_T
Cuts at: 1599 2703 3680 1599
Size: 1104 977 2166
Fragments arranged by size:
2166 1104 977
AgeI A'CCGG_T
Cuts at: 2635 2635
Size: 4247
AlwNI CAG_nnn'CTG
Cuts at: 266 378 411 1056 1183 1552 3119 266
Size: 112 33 645 127 369 1567 1394
Fragments arranged by size:
1567 1394 645 369 127 112 33
ApaLI G'TGCA_C
Cuts at: 716 3017 716
Size: 2301 1946
ApoI r'AATT_y
Cuts at: 1251 3600 3856 1251
Size: 2349 256 1642
Fragments arranged by size:
2349 1642 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4247
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4247
AvaI C'yCGr_G
Cuts at: 212 505 797 1267 212
Size: 293 292 470 3192
Fragments arranged by size:
3192 470 293 292
BamHI G'GATC_C
Cuts at: 1272 1272
Size: 4247
BanI G'GyrC_C
Cuts at: 1263 1677 1925 1960 1263
Size: 414 248 35 3550
Fragments arranged by size:
3550 414 248 35
BanII G_rGCy'C
Cuts at: 1261 2291 1261
Size: 1030 3217
BbeI G_GCGC'C
Cuts at: 1681 1929 1681
Size: 248 3999
BbsI GAAGACnn'nnnn_
Cuts at: 161 517 161
Size: 356 3891
BciVI GTATCCnnnnn_n'
Cuts at: 2145 2906 4247 2145
Size: 761 1341 2145
Fragments arranged by size:
2145 1341 761
BclI T'GATC_A
Cuts at: 1767 1767
Size: 4247
BglI GCCn_nnn'nGGC
Cuts at: 977 1156 977
Size: 179 4068
BglII A'GATC_T
Cuts at: 1762 3799 1762
Size: 2037 2210
BlpI GC'TnA_GC
Cuts at: 463 1359 463
Size: 896 3351
Bme1580I G_kGCm'C
Cuts at: 720 1872 1965 3021 720
Size: 1152 93 1056 1946
Fragments arranged by size:
1946 1152 1056 93
BmgBI CAC'GTC
Cuts at: 1047 1047
Size: 4247
BmrI ACTGGGnnnn_n'
Cuts at: 1459 1540 1875 4019 1459
Size: 81 335 2144 1687
Fragments arranged by size:
2144 1687 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 540 2614 540
Size: 2074 2173
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 370 433 787 1006 340
Size: 30 63 354 219 3581
Fragments arranged by size:
3581 354 219 63 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1423 2302 2794 3092 1423
Size: 879 492 298 2578
Fragments arranged by size:
2578 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3951 3951
Size: 4247
BsaAI yAC'GTr
Cuts at: 1600 1600
Size: 4247
BsaBI GATnn'nnATC
Cuts at: 6 1761 3798 6
Size: 1755 2037 455
Fragments arranged by size:
2037 1755 455
BsaHI Gr'CG_yC
Cuts at: 1064 1678 1926 2628 4124 1064
Size: 614 248 702 1496 1187
Fragments arranged by size:
1496 1187 702 614 248
BsaWI w'CCGG_w
Cuts at: 1436 1957 2635 2909 3056 3467 1436
Size: 521 678 274 147 411 2216
Fragments arranged by size:
2216 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 403 430 532 544 673 721 958 961 964
Size: 27 102 12 129 48 237 3 3
Cuts at: 964 1186 403
Size: 222 3464
Fragments arranged by size:
3464 237 222 129 102 48 27 12
3 3
BseYI C'CCAG_C
Cuts at: 398 608 1672 2430 3007 3503 398
Size: 210 1064 758 577 496 1142
Fragments arranged by size:
1142 1064 758 577 496 210
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1069 1069
Size: 4247
BsiEI CG_ry'CG
Cuts at: 104 843 1789 1835 3043 104
Size: 739 946 46 1208 1308
Fragments arranged by size:
1308 1208 946 739 46
BsiHKAI G_wGCw'C
Cuts at: 720 1261 2039 2229 3021 720
Size: 541 778 190 792 1946
Fragments arranged by size:
1946 792 778 541 190
BsmBI CGTCTCn'nnnn_
Cuts at: 154 154
Size: 4247
Bsp1286I G_dGCh'C
Cuts at: 720 1261 1872 1965 2039 2229 2291 3021 720
Size: 541 611 93 74 190 62 730 1946
Fragments arranged by size:
1946 730 611 541 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3467 3467
Size: 4247
BspHI T'CATG_A
Cuts at: 3423 3423
Size: 4247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 720 1168 1297 1511 1813 2194 720
Size: 448 129 214 302 381 2773
Fragments arranged by size:
2773 448 381 302 214 129
BsrBI CCG'CTC
Cuts at: 50 393 2539 2593 4077 4242 50
Size: 343 2146 54 1484 165 55
Fragments arranged by size:
2146 1484 343 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2159 2159
Size: 4247
BsrFI r'CCGG_y
Cuts at: 2245 2426 2635 2245
Size: 181 209 3857
Fragments arranged by size:
3857 209 181
BssSI C'ACGA_G
Cuts at: 2518 2876 2518
Size: 358 3889
BstAPI GCAn_nnn'nTGC
Cuts at: 1056 1624 1056
Size: 568 3679
BstBI TT'CG_AA
Cuts at: 2608 2608
Size: 4247
BstXI CCAn_nnnn'nTGG
Cuts at: 675 675
Size: 4247
BstYI r'GATC_y
Cuts at: 1 1272 1739 1762 2097 2343 3355 3441 3453
Size: 1271 467 23 335 246 1012 86 12
Cuts at: 3453 3799 1
Size: 346 449
Fragments arranged by size:
1271 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1013 1013
Size: 4247
BtgI C'CryG_G
Cuts at: 107 107
Size: 4247
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1235 2197 2378 2541 1235
Size: 962 181 163 2941
Fragments arranged by size:
2941 962 181 163
BtsI GCAGTG_nn'
Cuts at: 1490 1490
Size: 4247
DraI TTT'AAA
Cuts at: 1246 1246
Size: 4247
DraIII CAC_nnn'GTG
Cuts at: 651 651
Size: 4247
DrdI GACnn_nn'nnGTC
Cuts at: 1953 2811 1953
Size: 858 3389
EaeI y'GGCC_r
Cuts at: 182 290 326 773 1832 2006 2397 2424 3769
Size: 108 36 447 1059 174 391 27 1345
Cuts at: 3769 182
Size: 660
Fragments arranged by size:
1345 1059 660 447 391 174 108 36
27
EagI C'GGCC_G
Cuts at: 1832 1832
Size: 4247
EarI CTCTTCn'nnn_
Cuts at: 196 430 2270 2480 196
Size: 234 1840 210 1963
Fragments arranged by size:
1963 1840 234 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2455 2765 2911 4057 2455
Size: 310 146 1146 2645
Fragments arranged by size:
2645 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1259 1259
Size: 4247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 340 370 433 462 787 1006 2072 2504
Size: 9 30 63 29 325 219 1066 432
Cuts at: 2504 3251 3519 331
Size: 747 268 1059
Fragments arranged by size:
1066 1059 747 432 325 268 219 63
30 29 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1012 1012
Size: 4247
EcoO109I rG'GnC_Cy
Cuts at: 1134 1180 1188 1386 1134
Size: 46 8 198 3995
Fragments arranged by size:
3995 198 46 8
EcoRI G'AATT_C
Cuts at: 1251 1251
Size: 4247
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2266 2266
Size: 4247
FspI TGC'GCA
Cuts at: 382 424 916 2028 382
Size: 42 492 1112 2601
Fragments arranged by size:
2601 1112 492 42
HaeII r_GCGC'y
Cuts at: 357 1113 1681 1929 2951 3930 357
Size: 756 568 248 1022 979 674
Fragments arranged by size:
1022 979 756 674 568 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 825 857 2089 2119 2121 2151 825
Size: 32 1232 30 2 30 2921
Fragments arranged by size:
2921 1232 32 30 30 2
HincII GTy'rAC
Cuts at: 1286 1286
Size: 4247
KasI G'GCGC_C
Cuts at: 1677 1925 1677
Size: 248 3999
KpnI G_GTAC'C
Cuts at: 1267 1267
Size: 4247
MluI A'CGCG_T
Cuts at: 3680 3680
Size: 4247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2918 3102 2918
Size: 184 4063
MscI TGG'CCA
Cuts at: 775 2008 775
Size: 1233 3014
MslI CAynn'nnrTG
Cuts at: 139 2236 2363 139
Size: 2097 127 2023
Fragments arranged by size:
2097 2023 127
MspA1I CmG'CkG
Cuts at: 197 378 608 1358 1672 2032 3045 3290 197
Size: 181 230 750 314 360 1013 245 1154
Fragments arranged by size:
1154 1013 750 360 314 245 230 181
NaeI GCC'GGC
Cuts at: 2428 2428
Size: 4247
NarI GG'CG_CC
Cuts at: 1678 1926 1678
Size: 248 3999
NcoI C'CATG_G
Cuts at: 107 107
Size: 4247
NgoMIV G'CCGG_C
Cuts at: 2426 2426
Size: 4247
NspI r_CATG'y
Cuts at: 444 600 1107 1300 1800 2707 444
Size: 156 507 193 500 907 1984
Fragments arranged by size:
1984 907 507 500 193 156
PciI A'CATG_T
Cuts at: 2703 2703
Size: 4247
PflMI CCAn_nnn'nTGG
Cuts at: 852 852
Size: 4247
PfoI T'CCnGG_A
Cuts at: 764 764
Size: 4247
PmeI GTTT'AAAC
Cuts at: 1246 1246
Size: 4247
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3386 3418 3386
Size: 32 4215
PpuMI rG'GwC_Cy
Cuts at: 1134 1180 1188 1134
Size: 46 8 4193
Fragments arranged by size:
4193 46 8
PshAI GACnn'nnGTC
Cuts at: 748 748
Size: 4247
PstI C_TGCA'G
Cuts at: 1061 1294 1979 1061
Size: 233 685 3329
Fragments arranged by size:
3329 685 233
PvuI CG_AT'CG
Cuts at: 104 843 104
Size: 739 3508
PvuII CAG'CTG
Cuts at: 378 1672 2032 378
Size: 1294 360 2593
Fragments arranged by size:
2593 1294 360
RsrII CG'GwC_CG
Cuts at: 2442 2442
Size: 4247
SacI G_AGCT'C
Cuts at: 1261 1261
Size: 4247
SalI G'TCGA_C
Cuts at: 1284 1284
Size: 4247
SanDI GG'GwC_CC
Cuts at: 1134 1188 1134
Size: 54 4193
SapI GCTCTTCn'nnn_
Cuts at: 430 2270 2480 430
Size: 1840 210 2197
Fragments arranged by size:
2197 1840 210
SbfI CC_TGCA'GG
Cuts at: 1294 1294
Size: 4247
ScaI AGT'ACT
Cuts at: 3764 3764
Size: 4247
SexAI A'CCwGG_T
Cuts at: 647 674 647
Size: 27 4220
SfcI C'TryA_G
Cuts at: 33 117 1057 1290 1975 2968 3159 33
Size: 84 940 233 685 993 191 1121
Fragments arranged by size:
1121 993 940 685 233 191 84
SfiI GGCCn_nnn'nGGCC
Cuts at: 977 1156 977
Size: 179 4068
SfoI GGC'GCC
Cuts at: 1679 1927 1679
Size: 248 3999
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4247
SmaI CCC'GGG
Cuts at: 214 799 1269 214
Size: 585 470 3192
Fragments arranged by size:
3192 585 470
SmlI C'TyrA_G
Cuts at: 1402 2317 2809 3071 1402
Size: 915 492 262 2578
Fragments arranged by size:
2578 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4247
SphI G_CATG'C
Cuts at: 444 600 1300 1800 444
Size: 156 700 500 2891
Fragments arranged by size:
2891 700 500 156
StyI C'CwwG_G
Cuts at: 107 1160 1381 1735 107
Size: 1053 221 354 2619
Fragments arranged by size:
2619 1053 354 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1830 1963 2422 2631 1830
Size: 133 459 209 3446
Fragments arranged by size:
3446 459 209 133
TatI w'GTAC_w
Cuts at: 848 3762 848
Size: 2914 1333
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3764 3796 3764
Size: 32 4215
Tth111I GACn'n_nGTC
Cuts at: 640 988 2044 640
Size: 348 1056 2843
Fragments arranged by size:
2843 1056 348
XbaI T'CTAG_A
Cuts at: 1278 1278
Size: 4247
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1074 1742 1074
Size: 668 3579
XmaI C'CCGG_G
Cuts at: 212 797 1267 212
Size: 585 470 3192
Fragments arranged by size:
3192 585 470
XmnI GAAnn'nnTTC
Cuts at: 1030 1205 1030
Size: 175 4072
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI
BsiWI BsmI BsrGI BssHII BstEII BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PsiI PspOMI
PspXI PsrI SacII SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI